Article
Biochemistry & Molecular Biology
Alexandra M. Simond, Tung Bui, Dongmei Zuo, Virginie Sanguin-Gendreau, Trisha Rao, Wayne A. Phillips, Robert D. Cardiff, William J. Muller
Summary: This study found that the p110 alpha(HR) mutation attenuates metastatic behavior and is associated with the formation of ductal carcinoma in situ (DCIS) in ErbB2-driven breast cancer.
Article
Biochemistry & Molecular Biology
Chiara M. A. Cefalo, Teresa Mezza, Andrea Giaccari, Rohit N. Kulkarni
Summary: This study compared four methods for RNA extraction and found that the RNeasy MicroKit + Carrier (Qiagen) method provided the highest yield and purity.
Article
Pharmacology & Pharmacy
Yan Luo, Liwei Liu, Jihua Zhao, Yue Jiao, Meiyu Zhang, Guangli Xu, Yumao Jiang
Summary: In this study, it was found that inhibition of the PI3K/AKT1 signaling pathway by sinomenine (SIN) induces apoptosis in hepatocellular carcinoma (HCC) cells and inhibits cell proliferation. These findings provide a new direction for the treatment of HCC.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Cell Biology
Xiaodan Zhang, Chuansheng Hu, Chen Huang, Ying Wei, Xiaowei Li, Miaomiao Hu, Hua Li, Ji Wu, Daniel M. Czajkowsky, Yan Guo, Zhifeng Shao
Summary: This method presents a robust approach for acquiring finely resolved transcriptional programs from clinical tissues using immunofluorescence-guided laser capture microdissection. It allows analysis of transcriptional networks and signaling pathways of specific cell types within native tissues. The use of a small molecule inhibitor, RVC, protects the RNA from degradation during the procedure.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Xinyu Yang, Yinggai Song, TianYu Liang, Qiqi Wang, Ruoyu Li, Wei Liu
Summary: Coccidioidomycosis is increasing among Chinese patients, with challenges in diagnosis due to lack of specificity in clinical presentations and imaging findings. Diagnosis is primarily confirmed through histopathological examination, and the rarity of endemic mycoses along with limited availability of diagnostic tests poses challenges. The use of LCM and molecular techniques has shown improvement in species identification for timely diagnosis.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Oncology
Kristina Navrazhina, Sandra Garcet, Samuel C. Williams, Nicholas Gulati, Felix Kiecker, John W. Frew, Hiroshi Mitsui, James G. Krueger
Summary: The study demonstrates the importance of using laser capture microdissection (LCM) to accurately identify molecular changes in melanoma samples, without being affected by inflammatory infiltration. LCM analysis identified a greater number of differentially expressed genes, including melanocyte-specific genes, and revealed pathways related to cell proliferation.
PIGMENT CELL & MELANOMA RESEARCH
(2023)
Article
Plant Sciences
Wei Qin, Yongpeng Li, Bowen Peng, Hang Liu, Tiantian Chen, Xin Yan, Yaojie Zhang, Chen Wang, Xinghao Yao, Xueqing Fu, Ling Li, Kexuan Tang
Summary: In this study, a method using laser capture microdissection (LCM) was developed to isolate glandular secretory trichomes (GSTs) from leaf epidermis without fixation. The method efficiently captured GSTs and enriched for related metabolites. This approach enables the study of pure plant cell populations and has the potential to discover novel secondary metabolites.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Liqing Lu, Haijian Yang, Yanhui Xu, Li Zhang, Juxun Wu, Hualin Yi
Summary: This study used RNA-seq and Pacbio-seq technologies to analyze the transcriptome and plant hormone signals in citrus seed development, revealing the important roles of hormone signaling, cell division, and nutrient metabolism processes. Additionally, several genes, including CrWRKY74, were found to play critical roles in seed abortion.
Article
Pharmacology & Pharmacy
Shashank Pandey, Zdenek Tuma, Tereza Smrhova, Miroslava Cedikova, Tereza Macanova, Magdalena Chottova Dvorakova
Summary: A platform for precise proteomic profiling of targeted cell populations from heterogeneous tissue sections is developed using LCM and CAP-IA integration, showing effectiveness for handling very small tissue sections.
Article
Biochemical Research Methods
Jongmin Woo, Madeline Schoenfeld, Xinguo Sun, Thierry Iraguha, Zhanxiang Zhou, Qibin Zhang
Summary: Using a simplified method, we conducted a proteomics study of intestinal tissue enriched with Paneth cells and identified several proteins associated with Paneth cell characteristics.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Oncology
Lou Romanens, Prasad Chaskar, Rachel Marcone, Stephan Ryser, Jean-Christophe Tille, Raphael Genolet, Ketty Heimgartner-Hu, Killian Heimgartner, Jonathan S. Moore, Nicolas Liaudet, Guerkan Kaya, Mikael J. Pittet, Pierre-Yves Dietrich, Mauro Delorenzi, Daniel E. Speiser, Alexandre Harari, Petros Tsantoulis, Sana Intidhar Labidi-Galy
Summary: The spatial distribution of tumor-infiltrating lymphocytes (TIL) can predict breast cancer outcome and response to treatment. A new method called ST-FFPE has been developed to analyze formalin-fixed paraffin-embedded samples, allowing for the study of archival tissue. By examining different tumor compartments, this method can characterize the cellular composition of the tumor microenvironment.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Oncology
Yao Wang, Faqing Liang, Yuting Zhou, Juanjuan Qiu, Qing Lv, Zhenggui Du
Summary: This study investigated the pathogenesis of breast cancer by analyzing the dynamic expression changes of hub genes from normal mammary epithelium to different stages of breast disease. The analysis revealed that hub genes associated with breast cancer were gradually upregulated from NME to DCIS and then downregulated in IDC. Failure to downregulate these hub genes from DCIS to IDC was associated with poor patient survival. Further mechanistic studies are needed to explore these findings.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, General & Internal
Anca Marcu, Diana Nitusca, Adrian Vaduva, Flavia Baderca, Natalia Cireap, Dorina Coricovac, Cristina Adriana Dehelean, Edward Seclaman, Razvan Ilina, Catalin Marian
Summary: In this study, lncRNA profiling was conducted on TNBC and luminal A tissue samples from 18 BC patients. Although two lncRNAs showed differential expression in the profiling analysis, they did not reach statistical significance in individual validation. GAS5 lncRNA was identified as having differential expression in TNBC tissues and cells compared to luminal A, suggesting possible implications in the molecular mechanisms of the TNBC subtype. Further studies with larger cohort sizes are needed to assess the biomarker potential of GAS5 lncRNA in BC.
MEDICINA-LITHUANIA
(2021)
Article
Multidisciplinary Sciences
Xinjia Cai, Heyu Zhang, Jianyun Zhang, Tiejun Li
Summary: Oral submucous fibrosis (OSF) is a common potentially malignant disorder in the oral cavity, and researchers have proposed a method using laser capture microdissection to obtain epithelial and mesenchymal cells for studying the molecular mechanisms of this disease.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemistry & Molecular Biology
Ju Zhou, Saber Imani, Marzieh Dehghan Shasaltaneh, Shuguang Liu, Tao Lu, Junjiang Fu
Summary: This study evaluated the antitumor effect of thymoquinone, an active component in Nigella sativa, on two PIK3CA hotspot mutations in breast cancer cells and investigated the mechanistic effects of these mutations on the PI3K/Akt1 pathway using molecular dynamics. The findings demonstrated that the mutations reduced the inhibitory effect of Delta Np63 alpha on PIK3CA, leading to enhanced activity of the PI3K downstream signals.
MOLECULAR BIOLOGY REPORTS
(2022)
Letter
Oncology
Jonathan Cohen, Alison Palumbo, Jason Wing, Michael C. Heinrich
LEUKEMIA & LYMPHOMA
(2020)
Article
Oncology
Jean-Yves Blay, Cesar Serrano, Michael C. Heinrich, John Zalcberg, Sebastian Bauer, Hans Gelderblom, Patrick Schoffski, Robin L. Jones, Steven Attia, Gina D'Amato, Ping Chi, Peter Reichardt, Julie Meade, Kelvin Shi, Rodrigo Ruiz-Soto, Suzanne George, Margaret von Mehren
Article
Oncology
Jumpei Kondo, Won Jae Huh, Jeffrey L. Franklin, Michael C. Heinrich, Brian P. Rubin, Robert J. Coffey
JOURNAL OF PATHOLOGY
(2020)
Article
Oncology
Susanne Grunewald, Lillian R. Klug, Thomas Muhlenberg, Jonas Lategahn, Johanna Falkenhorst, Ajia Town, Christiane Ehrt, Eva Wardelmann, Wolfgang Hartmann, Hans-Ulrich Schildhaus, Juergen Treckmann, Jonathan A. Fletcher, Sascha Jung, Paul Czodrowski, Stephen Miller, Oleg Schmidt-Kittler, Daniel Rauh, Michael C. Heinrich, Sebastian Bauer
Summary: Avapritinib shows substantial clinical activity in patients with PDGFRA D842V genotype, but secondary resistance has been observed. Some PDGFRA mutant patients develop secondary mutations that interfere with avapritinib binding, leading to resistance.
Article
Oncology
Suzanne George, Robin L. Jones, Sebastian Bauer, Yoon-Koo Kang, Patrick Schoffski, Ferry Eskens, Olivier Mir, Phillipe A. Cassier, Cesar Serrano, William D. Tap, Jonathan Trent, Piotr Rutkowski, Shreyaskumar Patel, Sant P. Chawla, Eval Meiri, Michael Gordon, Teresa Zhou, Maria Roche, Micahel C. Heinrich, Margaret von Mehren
Summary: The study showed that avapritinib, as a fourth-line or later treatment in patients with GIST harboring KIT or PDGFRA mutations, has manageable toxicity with meaningful clinical activity in some patients.
Article
Medicine, Research & Experimental
Shuai Ye, Dinara Sharipova, Marya Kozinova, Lilli Klug, Jimson D'Souza, Martin G. Belinsky, Katherine J. Johnson, Margret B. Einarson, Karthik Devarajan, Yan Zhou, Samuel Litwin, Michael C. Heinrich, Ronald DeMatteo, Margaret von Mehren, James S. Duncan, Lori Rink
Summary: The management of Gastrointestinal Stromal Tumors (GISTs) has been greatly influenced by the identification of activating mutations in KIT and PDGFRA and the clinical application of RTK inhibitors. Despite the effectiveness of these inhibitors in most cases, resistance remains a clinical challenge, necessitating the identification of novel targets for refractory GISTs. Global kinome profiling shows promise in uncovering critical signaling networks and essential protein kinases in GISTs.
Article
Oncology
Robin L. Jones, Cesar Serrano, Margaret von Mehren, Suzanne George, Michael C. Heinrich, Yoon-Koo Kang, Patrick Schoffski, Philippe A. Cassier, Olivier Mir, Sant P. Chawla, Ferry A. L. M. Eskens, Piotr Rutkowski, William D. Tap, Teresa Zhou, Maria Roche, Sebastian Bauer
Summary: The study showed that targeting PDGFRA D842V-mutant GIST with avapritinib resulted in unprecedented, durable clinical benefit and manageable safety profile, suggesting avapritinib may be considered as first-line therapy for these patients.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Biochemistry & Molecular Biology
Hyunho Yoon, Chih-Min Tang, Sudeep Banerjee, Mayra Yebra, Sangkyu Noh, Adam M. Burgoyne, Jorge De la Torre, Martina De Siena, Mengyuan Liu, Lillian R. Klug, Yoon Young Choi, Mojgan Hosseini, Antonio L. Delgado, Zhiyong Wang, Randall P. French, Andrew Lowy, Ronald P. DeMatteo, Michael C. Heinrich, Alfredo A. Molinolo, J. Silvio Gutkind, Olivier Harismendy, Jason K. Sicklick
Summary: This study identified a paracrine signaling network in the tumor microenvironment where cancer-associated fibroblasts drive GIST growth and metastasis through the production of PDGFC. Targeting CAFs with a dual PI3K/mTOR inhibitor synergized with imatinib to increase tumor cell killing. This new cellular target for GIST therapy may improve disease control and cure rates.
Article
Oncology
Margaret von Mehren, Michael C. Heinrich, Hongliang Shi, Sergio Iannazzo, Raymond Mankoski, Sasa Dimitrijevic, Gerard Hoehn, Silvia Chiroli, Suzanne George
Summary: Avapritinib demonstrated more durable survival outcomes compared with other TKIs in patients with unresectable/metastatic PDGFRA D842V-mutant GIST, showing significant clinical activity and a favorable overall survival rate.
Article
Oncology
Lillian R. Klug, Christopher L. Corless, Michael C. Heinrich
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Oncology
Allen Li, Jamie M. Keck, Swapnil Parmar, Janice Patterson, Marilyne Labrie, Allison L. Creason, Brett E. Johnson, Molly Downey, George Thomas, Carol Beadling, Laura M. Heiser, Annette Kolodzie, Alexander R. Guimaraes, Christopher L. Corless, Joe W. Gray, Gordon B. Mills, Raymond C. Bergan, Zahi I. Mitri
Summary: The study describes an integrative approach to understanding the clinical significance of heterogeneity in breast cancer between metastatic lesions using multi-omic analyses of serial biopsies, aiming to improve cancer management. The researchers found remarkable heterogeneity among metastatic lesions in patients with metastatic breast cancer, resulting in discordance in receptor status and genomic alterations and mixed treatment responses.
NPJ PRECISION ONCOLOGY
(2021)
Article
Oncology
Sebastian Bauer, Michael C. Heinrich, Suzanne George, John R. Zalcberg, Cesar Serrano, Hans Gelderblom, Robin L. Jones, Steven Attia, Gina D'Amato, Ping Chi, Peter Reichardt, Julie Meade, Ying Su, Rodrigo Ruiz-Soto, Jean-Yves Blay, Margaret von Mehren, Patrick Schoffski
Summary: This study reports the genomic heterogeneity of tumors from patients with advanced GIST and evaluates the efficacy of ripretinib across different KIT/PDGFRA mutation subgroups. Ripretinib demonstrated progression-free survival benefit in all assessed subgroups, including those with wild-type KIT/PDGFRA by tumor tissue.
CLINICAL CANCER RESEARCH
(2021)
Review
Oncology
Lillian R. Klug, Homma M. Khosroyani, Jason D. Kent, Michael C. Heinrich
Summary: When Gastrointestinal Stromal Tumour (GIST) was identified as a distinct pathological entity, effective medical therapies were lacking, leading to poor prognosis. However, the discovery of KIT mutations and the development of TKIs revolutionized GIST treatment. Although there are approved drugs for treating advanced-stage GIST, challenges still remain and new therapeutic approaches are needed.
NATURE REVIEWS CLINICAL ONCOLOGY
(2022)
Review
Pharmacology & Pharmacy
Homma M. Khosroyani, Lillian R. Klug, Michael C. Heinrich
Summary: Prior to the early 2000s, patients with advanced GIST had poor prognoses due to lack of effective therapies. Tyrosine kinase inhibitors significantly improved the overall survival for these patients. This review discusses the studies that led to the approval of specific targeted therapies for different molecular subtypes of GIST, as well as their impact on future therapeutic approaches.
Article
Oncology
Suzanne George, Michael C. Heinrich, Neeta Somaiah, Peter Oppelt, Robert McLeod, Satoshi Nishioka, Madan G. Kundu, Xiaozhong Qian, Prasanna Kumar, Abderrahmane Laadem, Yvonne Lau, Brittany P. Tran, Maura Fallon, Ololade Dosunmu, Julia Shi, Yoichi Naito
Summary: This study evaluated the safety and tolerability of DS-6157a in treating GIST. The results showed that DS-6157a was well-tolerated, but had lower efficacy outcomes than anticipated.
CLINICAL CANCER RESEARCH
(2023)