Article
Cell Biology
Renjie Liao, Manas Mondal, Christopher D. Nazaroff, Diego Mastroeni, Paul D. Coleman, Joshua Labaer, Jia Guo
Summary: The method presented achieves highly sensitive and multiplexed protein analysis using cleavable fluorescent tyramide and off-the-shelf antibodies, enhancing detection sensitivity and reducing imaging time. By studying genetically identical cell populations and profiling neurons in human brain tissue, it reveals protein expression heterogeneity and the presence of distinct neuron clusters within different sub-regions of the hippocampus.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemical Research Methods
Limin Jiang, Hui Yu, Jiawei Li, Jijun Tang, Yan Guo, Fei Guo
Summary: The major histocompatibility complex (MHC) is valuable in treating complex human diseases. A review of 27 MHC I binding prediction tools revealed a common limitation in dealing with fixed peptide sequence length. In response to this limitation, a new approach called BVLSTM-MHC was developed, showing superior performance and a corresponding web server was created.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Biochemical Research Methods
Max Hoek, Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: The study analyzed in depth the N-glycosylation features of HLA class I molecules, finding that HLA-A alpha-chains have distinct glycosylation patterns compared to HLA-B, HLA-C, and HLA-F. The cellular distribution of HLA molecules varies based on their glycosylation features, with HLA-B predominantly found at the plasma membrane, and HLA-C and HLA-F mainly located in the endoplasmic reticulum and Golgi membrane. The findings highlight the importance of considering allotype-specific cellular distribution when analyzing peptide antigen presentation in immunopeptidomics.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
S. Youk, M. T. Le, M. Kang, B. Ahn, M. Choi, K. Kim, T. H. Kim, J. H. Kim, C. S. Ho, C. Park
Summary: A high-resolution typing method was developed for swine leukocyte antigen 3 (SLA-3), identifying 21 alleles with four novel ones, although the allelic diversity of SLA-3 was lower compared to SLA-1 and -2. More SLA-3 alleles were observed in Landrace and Yorkshire breeds, with SLA-3*04:01 being the most widely distributed allele across all breeds. This method aids in analyzing major SLA genes from field samples, enhancing understanding of immune responses and genetic makeup.
Article
Biochemical Research Methods
Raphael Trevizani, Zhen Yan, Jason A. Greenbaum, Alessandro Sette, Morten Nielsen, Bjoern Peters
Summary: An approach to assess the reliability of different metrics for evaluating the performance of MHC class I binding predictors was developed. The study found that using percentile-ranked results improved the stability of the ranks and identified the top-performing tools in the benchmark.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Nina Geng, Tuo Hu, Chunbo He
Summary: Immune checkpoint blockade therapies show durable responses in a subset of colorectal cancer patients, but the majority do not respond. Our study suggests that DDX60 may regulate MHC-I expression in colorectal cancer, and targeting DDX60 could potentially improve the efficacy of immunotherapy.
Review
Biochemistry & Molecular Biology
Akhil Shukla, Maryse Cloutier, Madanraj Appiya Santharam, Sheela Ramanathan, Subburaj Ilangumaran
Summary: The immune system constantly monitors and eliminates cancerous cells, with CD8(+) cytotoxic T lymphocytes playing a key role in tumor cell killing. Cancer cells evade immune surveillance by downregulating MHC-I and key proteins of the antigen processing and presentation machinery. NLRC5 has been identified as the key transcriptional activator of MHC-I and APM genes, and genetic lesions and epigenetic modifications of NLRC5 are common causes of MHC-I defects in cancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jeff D. Colbert, Freidrich M. Cruz, Christina E. Baer, Kenneth L. Rock
Summary: This study reveals a molecular association between Tspan5 and MHC I molecules that is critical for the formation of MHC I nanoclusters and optimal T cell responses to antigens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Cindy Dirscherl, Sara Loechte, Zeynep Hein, Janine-Denise Kopicki, Antonia Regina Harders, Noemi Linden, Andreas Karner, Johannes Preiner, Julian Weghuber, Maria Garcia-Alai, Charlotte Uetrecht, Martin Zacharias, Jacob Piehler, Peter Lanzerstorfer, Sebastian Springer
Summary: MHC-I FHC dimers are the prevalent species at the plasma membrane, with a tendency to cluster into higher order oligomers. This clustering leads to a moderate decrease in diffusion coefficient.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ewa Maria Sroka, Mathilde Lavigne, Marika Pla, Chrysoula Daskalogianni, Maria Camila Tovar-Fernandez, Rodrigo Prado Martins, Benedicte Manoury, Guillaume Darrasse-Jeze, Megane Nascimento, Sebastien Apcher, Robin Fahraeus
Summary: This study found that introducing an MHC class I epitope sequence into the intron of the beta-globin gene in mice can generate immune tolerance. Translation initiation within the intron produces peptides for MHC class I immune tolerance, providing an explanation for the tolerance of alternative tissue-specific splicing by the immune system.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Elena Shklovskaya, Helen Rizos
Summary: It is well acknowledged that the immune system plays a role in controlling tumor growth, but tumors can escape immune surveillance through mechanisms like downregulation or loss of MHC-I molecules. This review examines the dysregulation of MHC-I expression in cancer, the nature of MHC-I-bound antigenic peptides, and discusses therapeutic strategies to address MHC-I deficiency in solid tumors with a focus on NK cells and CD4 T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Eli Olson, Theadora Ceccarelli, Malini Raghavan
Summary: The extreme polymorphisms of HLA class I proteins enable diverse peptide presentation, and the endo-lysosomes in moDCs play a role in the presentation of HLA class I molecules. HLA-B allotypes display varying responses to disruption of the endo-lysosomal pH, with HLA-B*08:01 being more resistant and HLA-B*35:01 being more sensitive. The degree of endo-lysosomal assembly dictates constitutive HLA class I expression and affects the efficiency of cross-presentation.
Letter
Oncology
Tamara Steinfass, Juliane Poelchen, Qian Sun, Giovanni Mastrogiulio, Daniel Novak, Marlene Vierthaler, Sandra Pardo, Aniello Federico, Laura Hueser, Thomas Hielscher, Rafael Carretero, Rienk Offringa, Peter Altevogt, Viktor Umansky, Jochen Utikal
Summary: Melanoma is a deadly form of skin cancer with increasing incidence. Understanding the underlying mechanisms of resistance to treatment can enhance therapy efficacy and success.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2023)
Article
Oncology
Lei Zhan, Junhui Zhang, Jing Zhang, Xiaojing Liu, Suding Zhu, Yuchuan Shi, Yu He, Wenyan Wang, Yijing Wei, Zhenhai Tang, Guo Chen, Bing Wei, Yunxia Cao
Summary: In this study, the researchers found that upregulated autophagy and decreased expression of MHC-I and NLRC5 were associated with endometrial cancer (EC). Inhibition of autophagy was found to suppress MHC-I gene expression. They also discovered that LC3 interacted with NLRC5 to inhibit the NLRC5-mediated MHC-I antigen presentation pathway. These findings suggest that inhibiting LC3 and promoting NLRC5 may be a promising immunotherapy strategy for EC management.
Article
Immunology
Richel J. C. Bilderbeek, Maksim V. Baranov, Geert van den Bogaart, Frans Bianchi
Summary: This study reveals the over-presentation of membrane protein fragments in cytolytic and helper T cell responses. It also demonstrates the evolutionary conservation and lower frequency of mutations in transmembrane helices, suggesting that T cells are more tuned to respond to membrane proteins to avoid evasion by pathogens.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Bo Fu, Wei Long, Ying Zhang, Aifeng Zhang, Fengqin Miao, Yuqing Shen, Ning Pan, Guangming Gan, Fang Nie, Youji He, Jianqiong Zhang, Gaojun Teng
SCIENTIFIC REPORTS
(2015)
Article
Cell Biology
N. Pan, S. Lu, W. Wang, F. Miao, H. Sun, S. Wu, D. Nan, J. Qiu, J. Xu, J. Zhang
Article
Immunology
Fengqin Miao, Hang Sun, Ning Pan, Jinhuan Xu, Jie Qiu, Yuqing Shen, Wei Xie, Jianqiong Zhang
CLINICAL & DEVELOPMENTAL IMMUNOLOGY
(2013)
Article
Immunology
Ning Pan, Keping Chen, Jie Qiu, Hang Sun, Jinhuan Xu, Fengqin Miao, Qian Shi, Wei Jiang, Hui Jin, Youji He, Wei Xie, Jianqiong Zhang
Article
Multidisciplinary Sciences
Ning Pan, Wei Jiang, Hang Sun, Fengqin Miao, Jie Qiu, Hui Jin, Jinhuan Xu, Qian Shi, Wei Xie, Jianqiong Zhang
Article
Nanoscience & Nanotechnology
Fang Huang, Jinjin Zhao, Yiting Wei, Zhifa Wen, Yue Zhang, Xuru Wang, Yanfei Shen, Li-xin Wang, Ning Pan
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2020)
Article
Neurosciences
Xiaomei Lin, Tianyuyi Feng, Erheng Cui, Yunfei Li, Zhang Qin, Xiaohu Zhao
Summary: This study successfully established a rat model based on the genetic-environmental interaction, which exhibited phenotype characteristics similar to human AD in terms of cognitive function, brain microstructure, and immunohistochemistry. The genetic factor (APP mutation) and the environmental factor (acrolein exposure) accounted for 39.74% and 33.3% of the AD-like phenotypes in the model, respectively.
Article
Neurosciences
Gustavo Guimara Guerrero, Giovanna Bignoto Minhoto, Camilla dos Santos Tiburcio-Machado, Itza Amarisis Ribeiro Pinto, Claudio Antonio Federico, Marcia Carneiro Valera
Summary: The present study evaluated the influence of head and neck radiotherapy on the behavior and body weight gain in Wistar rats. The results demonstrated that different doses of radiation induced depressive behavior in the animals, and that the weight gain tended to be lower in the irradiated groups.
Article
Neurosciences
Ziwei Gao, Chao Lu, Yaping Zhu, Yuxin Liu, Yuesong Lin, Wenming Gao, Liyuan Tian, Lei Wu
Summary: This study reveals the underlying mechanisms of the rapid antidepressant effects of merazin hydrate (MH), which activates CaMKII to promote neuronal activities and proliferation in the hippocampus.
Article
Neurosciences
Kathleen E. Murray, Whitney A. Ratliff, Vedad Delic, Bruce A. Citron
Summary: Gulf War Illness (GWI) is a chronic disorder that affects approximately 30% of Veterans deployed to the Persian Gulf. This study found that exposure to toxicants during the Gulf War resulted in long-term changes in the morphology of dentate granule cells and that treatment with Nrf2 activator could improve neuronal health in the hippocampus.
Article
Neurosciences
Jing Li, Yan Zou, Xiangchuang Kong, Yangming Leng, Fan Yang, Guofeng Zhou, Bo Liu, Wenliang Fan
Summary: This study examines the functional connectivity changes in individuals with sudden sensorineural hearing loss (SSNHL) at the integrity, network, and edge levels. The findings reveal reduced intranetwork connectivity strength and increased internetwork connectivity in SSNHL patients. These alterations are associated with the duration of SSNHL and Tinnitus Handicap Inventory scores. The study provides crucial insights into the neural mechanisms of SSNHL and the brain's network-level responses to sensory loss.
Review
Neurosciences
Didier Majou, Anne-Lise Dermenghem
Summary: In the early stages of SAD, memory impairment is strongly correlated with cortical levels of soluble amyloid-beta peptide oligomers. A beta disrupts glutamatergic synaptic function and leads to cognitive deficits. This article describes the pathogenic mechanisms underlying cerebral amyloidosis, involving amyloid precursor protein synthesis, A beta residue clearance processes, and the role of specific molecules.
Article
Neurosciences
Jing Li, Yi Shan, Xiaojing Zhao, Guixiang Shan, Peng-Hu Wei, Lin Liu, Changming Wang, Hang Wu, Weiqun Song, Yi Tang, Guo-Guang Zhao, Jie Lu
Summary: This study investigates changes in brain anatomical structures and functional network connectivity after chronic complete thoracic spinal cord injury (cctSCI) and their impact on clinical outcomes. The findings reveal alterations in gray matter volume and functional connectivity in specific brain regions, indicating potential therapeutic targets and methods for tracking treatment outcomes.
Article
Neurosciences
Anllely Fernandez, Katherine Corvalan, Octavia Santis, Maxs Mendez-Ruette, Ariel Caviedes, Matias Pizarro, Maria -Teresa Gomez, Luis Federico Batiz, Peter Landgraf, Thilo Kahne, Alejandro Rojas-Fernandez, Ursula Wyneken
Summary: This study reveals the importance of SUMOylation in modulating the protein cargo of astrocyte-derived small extracellular vesicles (sEVs) and its potential impact on neurons.
Article
Neurosciences
Anika Luettig, Stefanie Perl, Maria Zetsche, Franziska Richter, Denise Franz, Marco Heerdegen, Ruediger Koehling, Angelika Richter
Summary: This study found that changes in c-Fos activity during short-term stimulation of the entopeduncular nucleus (EPN) are associated with improvement in dystonia, and also discovered that the cerebellum may be involved in the antidystonic effects.
Article
Neurosciences
Yanlin Tao, Wei Shen, Houyuan Zhou, Zikang Li, Ting Pi, Hui Wu, Hailian Shi, Fei Huang, Xiaojun Wu
Summary: Depression has a higher incidence in women compared to men, and this study investigated the impact of sex on depressive behaviors and underlying mechanisms using a corticosterone-induced depression model in mice. The results showed sex-specific anxiety and depression behaviors in the model group, as well as differences in protein expression and neurotransmitter levels between male and female mice. These findings enhance our understanding of sex-specific differences in depression and support tailored interventions.
Review
Neurosciences
Dnyandev G. Gadhave, Vrashabh V. Sugandhi, Chandrakant R. Kokare
Summary: This article discusses the characteristics and importance of the tight junctions of endothelial cells in the CNS, which act as a biological barrier known as the blood-brain barrier (BBB). It focuses on overcoming the challenges of delivering therapeutic agents to the brain in neurodegenerative disorders, particularly multiple sclerosis, through the use of biomaterials. The article also highlights the current limitations of animal models for studying multiple sclerosis and suggests a potential future research direction.
Article
Neurosciences
Li-Min Mao, Khyathi Thallapureddy, John Q. Wang
Summary: Propofol can enhance synapsin phosphorylation and modulate synaptic transmission in the mouse brain. The study reveals the potential role of synapsin as a substrate of propofol and its effects on neurotransmitter release machinery.
Article
Neurosciences
Syed Maaz Ahmed Rizvi, Abdul Baseer Buriro, Irfan Ahmed, Abdul Aziz Memon
Summary: This study explores the effects of prolonged mask usage on the human brain by analyzing EEG and physiological parameters. The results show that the mean EEG spectral power in alpha, beta, and gamma sub-bands of individuals wearing masks is smaller than those without masks. The performances on cognitive tasks and oxygen saturation level differ between the two groups, while blood pressure, body temperature, and heart rate are similar. The analysis also reveals that the occipital and frontal lobes exhibit the greatest variability in channel measurements.
Article
Neurosciences
Rui-Fang Ma, Lu-Lu Xue, Jin-Xiang Liu, Li Chen, Liu-Lin Xiong, Ting-Hua Wang, Fei Liu
Summary: This study observed changes in brain infarction and blood vessels in rats during neonatal hypoxic ischemic encephalopathy (NHIE) modeling using Transcranial Doppler Ultrasonography (TCD). Longer duration of hypoxia was associated with more severe nerve damage. TCD can dynamically monitor cerebral infarction after NHIE modeling, which may serve as a useful auxiliary method for evaluating animal experimental models.
Article
Neurosciences
Yuxiang Dai, Chen Yu, Lu Zhou, Longyang Cheng, Hongbin Ni, Weibang Liang
Summary: Overexpression of CXCR4 in glioma is correlated with patient survival, and its inhibition can reduce invasion and migration of glioma cells. Inhibiting Nur77 also decreases cancer progression associated with CXCR4.