Article
Biology
Anna E. Mammel, Heather Z. Huang, Amanda L. Gunn, Emma Choo, Emily M. Hatch
Summary: The length and gene density of the trapped chromosome are key factors determining the membrane stability and timing of rupture of micronuclei. Nuclear envelope composition defects only partly explain the membrane stability in micronuclei.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biochemistry & Molecular Biology
Kara J. Turner, Eleanor M. Watson, Benjamin M. Skinner, Darren K. Griffin
Summary: Infertility is a growing issue for many couples globally. Current marker tests for male factor infertility are complex and subjective. Research into better diagnostic methods is needed, with potential in morphology and telomere distribution as investigative tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
A. K. Balaji, Santam Saha, Shruti Deshpande, Darshini Poola, Kundan Sengupta
Summary: Dysregulation of chromatin remodeling and nuclear envelope proteins plays a significant role in cancer development. Recent advances in single-cell sequencing, imaging technologies, and data mining approaches enable the design of small molecules to selectively inhibit cancer cell growth and proliferation in a genome- and epigenome-specific manner.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Physics, Multidisciplinary
Kuang Liu, Alison E. Patteson, Edward J. Banigan, J. M. Schwarz
Summary: This study shows that the dynamics of chromosomes and nuclear shape in the cell nucleus come from motor activity, with a model of an active, cross-linked Rouse chain identifying the physical mechanisms. System-sized motions require both motor activity and cross-links, with contractile motors enhancing chromosome dynamics. Nuclear shape fluctuations are dependent on factors such as motor strength, cross-linking, and chromosome-lamina binding.
PHYSICAL REVIEW LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Sarah Mermet, Maxime Voisin, Joris Mordier, Tristan Dubos, Sylvie Tutois, Pierre Tuffery, Celia Baroux, Kentaro Tamura, Aline Probst, Emmanuel Vanrobays, Christophe Tatout
Summary: This study reveals the interaction mechanism between the Arabidopsis nucleoskeleton protein KAKU4 and the nuclear periphery, and identifies conserved peptide motifs that play a role in nuclear shape and gene expression. Additionally, the study finds these motifs in nuclear pore basket proteins, suggesting a physical continuum between the nuclear pore and the nucleoskeleton in plants.
Article
Cell Biology
Norma E. Padilla-Mejia, Ludek Koreny, Jennifer Holden, Marie Vancova, Julius Lukes, Martin Zoltner, Mark C. Field
Summary: The nuclear lamina plays crucial roles in maintaining nuclear structure and controlling gene expression. In trypanosomes, the N- and C-termini of NUP-1 act as interaction hubs and impact other components of the lamina and nuclear envelope.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Isabelle Loiodice, Mickael Garnier, Ivaylo Nikolov, Angela Taddei
Summary: Silent chromatin in eukaryotic cells is mainly located at the nuclear periphery, and the establishment of silencing is a stepwise process occurring over several cell cycles. Studying a budding yeast model, researchers have identified mechanisms and protein recruitment relationships during the process of establishing silencing at an ectopic locus.
Article
Biochemical Research Methods
Sarah M. Mangiameli, Haiqi Chen, Andrew S. Earl, Julie A. Dobkin, Daniel Lesman, Jason D. Buenrostro, Fei Chen
Summary: Photoselective sequencing is a new method for genomic and epigenomic profiling in morphologically distinct regions. It uses targeted illumination to selectively unblock a photocaged fragment library, enabling sequencing-based readout in microscopically identified spatial regions. The method was validated by analyzing chromatin accessibility profiles of fluorescently-labeled cell types in the mouse brain and comparing with published data. Photoselective sequencing is a flexible and generalizable platform for studying the interplay of spatial structures with genomic and epigenomic properties.
Article
Multidisciplinary Sciences
Cheryl L. Smith, Yemin Lan, Rajan Jain, Jonathan A. Epstein, Andrey Poleshko
Summary: The nuclear architecture of rod photoreceptor cells in nocturnal mammals differs from that of other animal cells, with euchromatin and heterochromatin occupying opposite positions. This unique structure is achieved through global relabeling of the rod cell epigenome, involving histone modifications such as H3K9 methylation. This study highlights the significance of epigenetic changes in shaping nuclear architecture in postmitotic cells.
Article
Multidisciplinary Sciences
Clara Lopes Novo, Emily Wong, Colin Hockings, Chetan Poudel, Eleanor Sheekey, Meike Wiese, Hanneke Okkenhaug, Simon J. Boulton, Srinjan Basu, Simon Walker, Gabriele S. Kaminski Schierle, Geeta J. Narlikar, Peter J. Rugg-Gunn
Summary: The authors demonstrate that satellite transcripts in mouse embryonic stem cells play a role in driving the formation of HP1α droplets and modulating the organization of heterochromatin. Depleting these transcripts leads to a less dynamic and more static state of heterochromatin and results in chromosome instability. This study highlights the importance of satellite transcripts in maintaining genome stability.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Koh Nakayama, Sigal Shachar, Elizabeth H. Finn, Hiroyuki Sato, Akihiro Hirakawa, Tom Misteli
Summary: Chromosome structure and nuclear organization play important roles in gene expression regulation. The relationship between the 3D position of a gene in the nucleus and its activity is still not clear. A study on nearly 100 hypoxia-responsive genes found that most of them are located in the intermediate region of the nucleus, and some of them change their position under hypoxic conditions. However, gene position changes are not directly related to gene activity.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Multidisciplinary Sciences
Toby Buttress, Shengbo He, Liang Wang, Shaoli Zhou, Gerhard Saalbach, Martin Vickers, Guohong Li, Pilong Li, Xiaoqi Feng
Summary: Based on the study, a histone variant H2B.8 in Arabidopsis thaliana is found to mediate sperm chromatin condensation. H2B.8 aggregates transcriptionally inactive AT-rich chromatin into condensates, facilitating nuclear compaction and maintaining active transcription, which is crucial for male transmission and fertility in flowering plants.
Article
Cell Biology
Raakhee Shankar, Molly M. Lettman, William Whisler, Elisa B. Frankel, Anjon Audhya
Summary: This study reveals the critical role of the ESCRT machinery in maintaining the structure of the inner nuclear membrane. Impaired ESCRT function results in a defect in pruning inner nuclear membrane invaginations, leading to perturbed nuclear envelope architecture and increased chromosome segregation defects, ultimately causing embryonic lethality.
Article
Cell Biology
Stephen P. Methot, Jan Padeken, Giovanna Brancati, Peter Zeller, Colin E. Delaney, Dimos Gaidatzis, Hubertus Kohler, Alexander van Oudenaarden, Helge Grosshans, Susan M. Gasser
Summary: The deposition of H3K9me2 and H3K9me3 can repress lineage-specific and germline genes in terminally differentiated Caenorhabditis elegans tissues by restricting the activity of specific transcription factors. Changes in H3K9me during development affect gene regulation and the maintenance of cellular functions.
NATURE CELL BIOLOGY
(2021)
Article
Biochemical Research Methods
Tristan Dubos, Axel Poulet, Geoffrey Thomson, Emilie Pery, Frederic Chausse, Christophe Tatout, Sophie Desset, Josien C. van Wolfswinkel, Yannick Jacob
Summary: In this study, an automated method called NODeJ is proposed for quantitative analysis of three-dimensional chromatin organization. NODeJ accurately identifies chromatin domains within nuclei and is applicable to different staining methods. It also allows for the analysis of specific targets, improving the efficiency of subnuclear structure analysis.
BMC BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Inma Gonzalez, Roberto Munita, Eneritz Agirre, Travis A. Dittmer, Katia Gysling, Tom Misteli, Reini F. Luco
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Inma Gonzalez, Roberto Munita, Eneritz Agirre, Travis A. Dittmer, Katia Gysling, Tom Misteli, Reini F. Luco
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2015)
Review
Critical Care Medicine
Matt Aldag, Regina C. Armstrong, Faris Bandak, Patrick S. F. Bellgowan, Timothy Bentley, Sean Biggerstaff, Katrina Caravelli, Joan Cmarik, Alicia Crowder, Thomas J. DeGraba, Travis A. Dittmer, Richard G. Ellenbogen, Colin Greene, Raj K. Gupta, Ramona Hicks, Stuart Hoffman, Robert C. Latta, Michael J. Leggieri, Donald Marion, Robert Mazzoli, Michael McCrea, John O'Donnell, Mark Packer, James B. Petro, Todd E. Rasmussen, Wendy Sammons-Jackson, Richard Shoge, Victoria Tepe, Ladd A. Tremaine, James Zheng
JOURNAL OF NEUROTRAUMA
(2017)
Article
Biochemistry & Molecular Biology
Molly S. Shook, Eric J. Richards
Article
Cell Biology
Travis A. Dittmer, Nidhi Sahni, Nard Kubben, David E. Hill, Marc Vidal, Rebecca C. Burgess, Vassilis Roukos, Tom Misteli
MOLECULAR BIOLOGY OF THE CELL
(2014)
Article
Biochemistry & Molecular Biology
Jeongsik Kim, Jin Hee Kim, Eric J. Richards, Kyung Min Chung, Hye Ryun Woo
Article
Plant Sciences
Junsik Choi, Susan R. Strickler, Eric J. Richards
Article
Cell Biology
Endia L. Blunt, Jason A. Shandler, Erika J. Hughes, Hayley Sussman, Rachel C. Christopherson, Eric J. Richards
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Clinical Neurology
Thomas J. DeGraba, Kathy Williams, Robert Koffman, Jennifer L. Bell, Wendy Pettit, James P. Kelly, Travis A. Dittmer, George Nussbaum, Geoffrey Grammer, Joseph Bleiberg, Louis M. French, Treven C. Pickett
Summary: This study presents the outcomes of an interdisciplinary intensive outpatient program (IOP) for military service members with combat-related mTBI and comorbid PH conditions. The IOP achieved significant and sustainable symptom recovery in patients, with improvements seen across various outcome measures at discharge and follow-up visits. The findings support further research and consideration of this care model for complex medical conditions.
FRONTIERS IN NEUROLOGY
(2021)
Editorial Material
Cell Biology
Junsik Choi, Eric J. Richards
Summary: The study reveals that the plant nuclear membrane protein PNET2 can bind histones and is involved in chromatin interactions at the nuclear edge.
DEVELOPMENTAL CELL
(2022)
Review
Clinical Neurology
Helen Phipps, Stefania Mondello, Arlington Wilson, Travis Dittmer, Natalie N. Rohde, Paul J. Schroeder, Jaime Nichols, Camille McGirt, Justin Hoffman, Kaila Tanksley, Mariam Chohan, Amanda Heiderman, Hussein Abou Abbass, Firas Kobeissy, Sidney Hinds
FRONTIERS IN NEUROLOGY
(2020)
Article
Biochemistry & Molecular Biology
Junsik Choi, Eric J. Richards
PLANT SIGNALING & BEHAVIOR
(2020)
Meeting Abstract
Zoology
Eric J. Richards, Natalic Henkhaus, Ila S. Anand
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2013)