Article
Cell Biology
Douglas M. Anderson, Kelly M. Anderson, Benjamin R. Nelson, John R. McAnally, Svetlana Bezprozvannaya, John M. Shelton, Rhonda Bassel-Duby, Eric N. Olson
Summary: CARDINAL, a cardiac lncRNA transcribed adjacent to myocardin, forms a nuclear complex with SRF and inhibits TCF-mediated transcription of the promitogenic gene c-fos, serving as an RNA cofactor for SRF in the heart. Deletion of CARDINAL results in ectopic TCF/SRF-dependent mitogenic gene expression and reduced cardiac contractility in response to age and ischemic stress in mice.
GENES & DEVELOPMENT
(2021)
Article
Biology
Colin J. Dinsmore, Philippe Soriano
Summary: Serum response factor (SRF) is a crucial transcription factor that regulates various cellular processes. It controls the expression of immediate early genes and actin cytoskeleton-related genes, which are involved in cell proliferation, migration, and differentiation. SRF coordinates these processes through the interaction with its cofactors, ternary complex factors (TCFs) and myocardin-related transcription factors (MRTFs). This study demonstrates the important role of MRTFs in mediating SRF function during cardiac neural crest development, providing new insights into the transcriptional regulation mechanism of SRF during development.
Review
Biochemistry & Molecular Biology
Mengyuan Wang, Qian Li, Lingbo Liu
Summary: Gene expression regulation involves multiple processes and various regulatory factors. This review provides an overview of key factors involved in gene expression regulation, such as transcription factors, chromatin accessibility, histone modifications, DNA methylation, and RNA modifications. Additionally, methods for detecting these regulatory factors are also described.
Article
Biochemistry & Molecular Biology
Lorela Ciraku, Zachary A. Bacigalupa, Jing Ju, Rebecca A. Moeller, Giang Le Minh, Rusia H. Lee, Michael D. Smith, Christina M. Ferrer, Sophie Trefely, Luke T. Izzo, Mary T. Doan, Wiktoria A. Gocal, Luca D'Agostino, Wenyin Shi, Joshua G. Jackson, Christos D. Katsetos, Kathryn E. Wellen, Nathaniel W. Snyder, Mauricio J. Reginato
Summary: This study identifies a novel mechanism in which OGT regulates acetate-dependent acetyl-CoA and lipid production by regulating phosphorylation of ACSS2 by CDK5. The OGT/CDK5/ACSS2 pathway may be a potential target for treating altered metabolic dependencies in brain tumors.
Article
Plant Sciences
Yuxin Shen, Zhengrui Qin, Gaojie Ren, Pingchuan Deng, Wanquan Ji, Chen Jiao, Liang Wu
Summary: Alternative splicing mediated by SCL33, SCL33L, and miR156 is involved in phase transitions in Brachypodium, regulating intron assembly and miR156 targets in growth transitions.
Article
Biochemistry & Molecular Biology
Reyna Sara Quintero-Barceinas, Franziska Gehringer, Charles Ducker, Janice Saxton, Peter E. Shaw
Summary: ELK-1 is a mitogen-responsive transcription factor that stimulates immediate early gene expression at the onset of the cell cycle and is subject to multiple levels of posttranslational control. Recent studies show that USP17 can enhance ELK-1 transcriptional activity, but contrary to FBXO25, it does not promote ELK-1 ubiquitination. This suggests the existence of another E3 ubiquitin ligase regulating ELK-1 ubiquitination and function.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Ulrik Kraemer Sundekilde, Caroline Maag Kristensen, Mette Algot Olsen, Henriette Pilegaard, Martin Kroyer Rasmussen
Summary: The circadian rhythm has a significant impact on various physiological processes, including sleep-wake patterns, eating behavior, and hepatic detoxification. This study investigated the role of hepatic peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1 alpha) in the circadian regulation of cytochrome P450 enzymes (Cyps) in the liver. The results showed that the expression of circadian regulator genes Bmal1 and Clock was influenced by PGC-1 alpha, and the mRNA content of certain Cyps exhibited rhythmic expression. However, no significant circadian regulation was observed at the protein level. Principal component analysis further demonstrated the distinct differences between the control and PGC-1a LKO mice. These findings provide valuable insights into the molecular mechanisms underlying circadian regulation of Cyps and hepatic detoxification.
Article
Biochemical Research Methods
Hui Hu, Qiong Zhang, Fei-Fei Hu, Chun-Jie Liu, An-Yuan Guo
Summary: Transcription factors (TFs) play crucial roles in biological processes and their expression patterns and regulatory mechanisms show differences in normal tissues and cancers, providing potential applications. Some TFs may serve as marker genes in specific tissues or cancers, while TFs from small families have central roles in transcriptional regulation.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Oliver Kuchler, Jule Gerlach, Thomas Vomhof, Johannes Hettich, Julia Steinmetz, J. Christof M. Gebhardt, Jens Michaelis, Bernd Knoell
Summary: In cells, proteins encoded by the same gene can exhibit spatial and temporal diversity due to functional subpopulations. Single-molecule tracking microscopy allows for high-resolution imaging and quantification of dynamic properties, providing insight into the behavior of transcription factors during cell stimulation and differentiation. This study focused on the serum response factor (SRF) in neuronal differentiation and revealed dynamic changes in DNA residence time, DNA-bound fraction, and nuclear positions of SRF clusters throughout the process.
Article
Multidisciplinary Sciences
Xiaoding Xu, Chuwei Yu, Hongxiu He, Xiangyu Pan, Aijun Hou, Jianxun Feng, Rongrong Tan, Likun Gong, Jing Chen, Jin Ren
Summary: Epigenetic regulations, particularly miR-337-3p, play a crucial role in the pathogenesis of metabolic-associated fatty liver disease (MAFLD). Overexpression of miR-337-3p can ameliorate hepatic lipid accumulation, reduce fasting blood glucose, and improve insulin resistance. Additionally, miR-337-3p may influence multiple genes involved in glycolipid metabolism, providing a potential therapeutic target for MAFLD.
Review
Endocrinology & Metabolism
Karine de Mattos, Robert S. Viger, Jacques J. Tremblay
Summary: Cell differentiation and acquisition of specialized functions are critical for tissue development and function. Transcription factors play a key role in regulating gene transcription in specific cell types. Several important transcription factors have been identified in Leydig cells, shedding light on their differentiation and function.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Guo-dong Huang, Ping Cui, Guo-Xu Ma, Fan-fan Chen, Ze-bin Chen, Xue-juan Li, Zi-jun Liao, Wei-ping Li, Zong-yang Li, Lei Chen
Summary: The new compound PGA shows cytotoxic effects on recurrent GBM cells and TMZ-resistant cell lines by inhibiting ELK1-SRF complex-dependent transcription, which leads to the suppression of downstream genes EGR1-BMI1 and the regulation of MCL1 and FBXW7. PGA could potentially be a therapeutic drug candidate for targeting the ELK1-SRF complex in the treatment of recurrent GBM.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Essam Darwish, Ritesh Ghosh, Abraham Ontiveros-Cisneros, Huy Cuong Tran, Marcus Petersson, Liesbeth De Milde, Martyna Broda, Alain Goossens, Alex Van Moerkercke, Kasim Khan, Olivier Van Aken
Summary: Plants respond to mechanical stimuli by regulating their growth and defense mechanisms. This study identifies the molecular mechanisms of touch signaling and reveals that Piezo ion channels do not play a major role in touch-induced gene expression and thigmomorphogenesis. Instead, the receptor-like kinase Feronia acts as a strong negative regulator of the touch signaling pathway dependent on jasmonic acid. Furthermore, the calmodulin-binding transcriptional activators CAMTA1/2/3 are key regulators of the touch signaling pathway independent of jasmonic acid.
Review
Neurosciences
Akiko Tabuchi, Daisuke Ihara
Summary: Accumulating evidence suggests that MKL/MRTF plays critical roles in various physiological and pathological processes in different cell types. These molecules translocate from the cytoplasm to the nucleus in association with actin rearrangement, regulating gene expression and cell motility. In the nervous system, MKL/MRTF is highly expressed in the brain, especially at synapses, influencing dendritic complexity and spine maturation.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Kevin G. Mark, Michael Rape
Summary: Research has shown that ubiquitylation plays a crucial role in transcription, ensuring the correct expression of genes during development. Disruption of these regulatory circuits can lead to a wide array of human diseases.
Review
Pharmacology & Pharmacy
Katarzyna Trzos, Natalia Pydyn, Jolanta Jura, Jerzy Kotlinowski
Summary: This review discusses the etiology, risk factors, and murine models of autoimmune liver diseases, providing important references for studying their development mechanisms and testing new treatment strategies.
PHARMACOLOGICAL REPORTS
(2022)
Article
Health Care Sciences & Services
Verena Rass, Bogdan-Andrei Ianosi, Laura Zamarian, Ronny Beer, Sabina Sahanic, Anna Lindner, Mario Kofler, Alois Josef Schiefecker, Philipp Mahlknecht, Beatrice Heim, Victoria Limmert, Thomas Sonnweber, Alex Pizzini, Piotr Tymoszuk, Christoph Scherfler, Atbin Djamshidian, Stefan Kiechl, Ivan Tancevski, Klaus Seppi, Bettina Pfausler, Judith Loeffler-Ragg, Raimund Helbok
Summary: The study found that about one-third of patients experienced impaired HR-QoL 3 months after COVID-19 diagnosis. Factors associated with impaired HR-QoL included younger age, longer hospitalization, impaired sleep, and anxiety symptoms.
QUALITY OF LIFE RESEARCH
(2022)
Article
Biology
Thomas Sonnweber, Piotr Tymoszuk, Sabina Sahanic, Anna Boehm, Alex Pizzini, Anna Luger, Christoph Schwabl, Manfred Nairz, Philipp Grubwieser, Katharina Kurz, Sabine Koppelstaetter, Magdalena Aichner, Bernhard Puchner, Alexander Egger, Gregor Hoermann, Ewald Woell, Guenter Weiss, Gerlig Widmann, Ivan Tancevski, Judith Loeffler-Ragg
Summary: This study observed the respiratory and symptom recovery in COVID-19 patients during the follow-up period. The results showed that 49% of patients reported persistent symptoms at the 6-month follow-up. Structural lung abnormalities and impaired lung function were more common in severe cases. The study also found that early clinical indicators could predict the risk of incomplete pulmonary recovery.
Article
Immunology
David Haschka, Verena Petzer, Francesco Robert Burkert, Gernot Fritsche, Sophie Wildner, Rosa Bellmann-Weiler, Piotr Tymoszuk, Guenter Weiss
Summary: This study characterized the features of neutrophils and monocyte subsets in COVID-19 patients within 72 hours of hospital admission, and found that inflammation-driven neutrophil expansion, depletion of CD16(+) nonclassical monocytes, and changes in surface marker expression were associated with the severity of the infection.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Judyta Gorka, Paulina Marona, Oliwia Kwapisz, Janusz Rys, Jolanta Jura, Katarzyna Miekus
Summary: This study investigated the role of MCPIP1 in ccRCC cell migration and found that MCPIP1 inhibits ccRCC cell migration by reducing the levels of Rho GTPases and the phosphorylation of FAK and Src. The loss of MCPIP1 RNase activity leads to increased expression of IL-1 beta and changes in the expression of genes correlated with migration as tumor progression occurs in ccRCC patients.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Katharina Huefner, Piotr Tymoszuk, Dietmar Ausserhofer, Sabina Sahanic, Alex Pizzini, Verena Rass, Matyas Galffy, Anna Boehm, Katharina Kurz, Thomas Sonnweber, Ivan Tancevski, Stefan Kiechl, Andreas Huber, Barbara Plagg, Christian J. Wiedermann, Rosa Bellmann-Weiler, Herbert Bachler, Gunter Weiss, Giuliano Piccoliori, Raimund Helbok, Judith Loeffler-Ragg, Barbara Sperner-Unterweger
Summary: COVID-19 outpatients are at higher risk of mental health issues following recovery, and specific acute symptoms may lead to deterioration of mental health. Early psychological and psychiatric interventions may be beneficial for these patients.
FRONTIERS IN MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Lukasz Kaniuk, Krzysztof Berniak, Agata Lichawska-Cies, Jolanta Jura, Joanna E. Karbowniczek, Urszula Stachewicz
Summary: The enormous potential of electrospun polymer fibers in the field of biomaterials for tissue engineering and wound healing has been explored in this study. Fibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffolds were created to support the anchoring and proliferation of keratinocytes. Hyaluronic acid (HA) was used as a coating on PHBV fibers to improve wound closure processes.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Cell Biology
Paulina Marona, Judyta Gorka, Oliwia Kwapisz, Jolanta Jura, Janusz Rys, Robert M. Hoffman, Katarzyna Miekus
Summary: The study reveals that resistance to sunitinib and sorafenib in renal cell carcinoma involves different mechanisms, resulting in increased malignancy. Sunitinib resistance enhances tumor angiogenesis, while sorafenib resistance disrupts endothelial cell integrity. Both resistance mechanisms involve MCPIP1 inhibition and ccRCC progression.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Weronika Sowinska, Mateusz Wawro, Debolina D. Biswas, Jakub Kochan, Katarzyna Pustelny, Aleksandra Solecka, Angela S. Gupta, Karli Mockenhaupt, Jaroslaw Polak, Borys Kwinta, Tomasz Kordula, Aneta Kasza
Summary: The precise functions of Regnase-2 (Reg-2/ZC3H12B/MCPIP2) and its regulatory mechanisms remain unclear. This study shows that Reg-2 actively controls neuroinflammation in nontransformed cells, particularly primary astrocytes. It downregulates the mRNA levels of proinflammatory cytokines IL-1 beta and IL-6, as well as regulates the expression of Regnase-1 (Reg-1/ZC3H12A/MCPIP1). The expression levels of Reg-2 and Reg-1 are inversely related and their imbalance is associated with glioblastoma progression and patient prognosis.
Article
Gastroenterology & Hepatology
Natalia Pydyn, Justyna Kadluczka, Piotr Major, Tomasz Hutsch, Kinga Belamri, Piotr Malczak, Dorota Radkowiak, Andrzej Budzynski, Katarzyna Miekus, Jolanta Jura, Jerzy Kotlinowski
Summary: This study found that the protein levels of MCPIP1 are reduced in NAFLD patients.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Olga Mucha, Bozena Skupien-Rabian, Alicja Slota, Katarzyna Trzos, Natalia Pydyn, Bartosz Podlewski, Jolanta Jura, Jerzy Kotlinowski
Summary: MCPIP1 plays a regulatory role in adipogenesis and lipid metabolism, as well as in hepatocyte metabolism. Our study reveals that Mcpip1 deficiency leads to increased blood glucose levels during fasting and alters the expression of proteins involved in cellular adhesion and metabolic processes.
ACTA BIOCHIMICA POLONICA
(2023)
Article
Multidisciplinary Sciences
Agata Lichawska-Cieslar, Weronika Szukala, Tomasz K. Prajsnar, Niedharsan Pooranachandran, Maria Kulecka, Michalina Dabrowska, Michal Mikula, Krzysztof Rakus, Magdalena Chadzinska, Jolanta Jura
Summary: This study investigated the role of Mcpip1 in embryonic development using a zebrafish model. The expression of the zc3h12a gene encoding Mcpip1 was found to undergo dynamic changes during embryogenesis. Overexpression of wild-type Mcpip1 resulted in embryonic lethality and extensive changes in gene expression related to development.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Natascha Brigo, Christa Pfeifhofer-Obermair, Egon Demetz, Piotr Tymoszuk, Guenter Weiss
Summary: Macrophages play a crucial role in host defense against intracellular pathogens such as Salmonella. Through phagocytosis, macrophages can eliminate bacteria. Infection with Salmonella can cause gastrointestinal disorders and systemic infection.
Article
Biology
Christa Pfeifhofer-Obermair, Natascha Brigo, Piotr Tymoszuk, Guenter Weiss
Summary: Salmonella enterica serovar Typhimurium is a bacterium that causes gastrointestinal disorders and typhoid fever-like infections. We established an experimental model to study the role of different cell types in defending against bacterial infections.
Letter
Hematology
Verena Petzer, Normann Steiner, Olga Angelova-Unterberger, Gabriele Hetzenauer, Kathrin Philipp-Abbrederis, Ella Willenbacher, Clemens Feistritzer, Wolfgang Willenbacher, Jakob Rudzki, Reinhard Stauder, Florian Kocher, Andreas Seeber, Andreas Pircher, Piotr Tymoszuk, Christian Isara, Alexander Egger, Vilmos Fux, Markus Anliker, Eberhard Gunsilius, David Nachbaur, Stefan Schmidt, Dominik Wolf