Article
Multidisciplinary Sciences
Jing Tong, Manman Hu, Beibei Han, Yanhai Ji, Baoju Wang, Hao Liang, Mingchi Liu, Zhanhui Wu, Ning Liu
Summary: Chinese chive has unique flavor, nutrient, and medicinal values, and investigating its molecular mechanism on flavor formation and metabolic pathways is crucial. This study identified GLY1 and SKP1 as the most stable reference genes for normalization, highlighting the importance of validating reference gene stability before use. Using at least two reference genes for normalization can provide more accurate results, with the combination of GLY1 and SKP1 showing reliable normalization results in qRT-PCR experiments.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Pramoda Kumar Sahoo, Sonali Parida, Subhadarshini Parida, Priyashree Parida, Anirban Paul
Summary: Argulosis is a economically significant freshwater fish ectoparasitic disease. Proper selection or normalization of reference genes is crucial for the accuracy of gene expression studies using real-time PCR. This study evaluated seven candidate reference genes and conducted comprehensive gene expression analysis in rohu carp infected with Argulus siamensis. The results identified stable and unstable reference genes and showed the role of immune modulation process in governing infection.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Yongshun Zhou, Fanze Meng, Kai Han, Kaiyue Zhang, Jianfeng Gao, Fulong Chen
Summary: This study evaluated the expression stability of 9 endogenous reference genes of Chlorella sp. TLD 6B, a microalgae growing in the Taklamakan Desert, Xinjiang of China, under four abiotic stresses (drought, salt, cold and heat). The results showed that 18S, GTP, IDH and CYP were the most stable endogenous reference genes under different stress conditions, and UBC and 18S were the most suitable endogenous reference gene combinations for all samples. Using these reliable endogenous reference genes ensures more accurate RT-qPCR quantification, facilitating functional genomics studies of desert Chlorella and the mining of resistance genes.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Xiaoxing Yang, Guangxiang Tong, Le Dong, Ting Yan, Huan Xu, Guopan Tang, Yongquan Zhang, Kai Ma, Jiasheng Yin, Youyi Kuang
Summary: This study evaluated the stability of 10 commonly used candidate reference genes in taimen and identified RPS29 and RPL19 as the optimal reference genes for qRT-PCR analysis of gene expression in taimen.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Long Ma, Ting Jiang, Xiangya Liu, Haijun Xiao, Yingchuan Peng, Wanna Zhang
Summary: This study evaluated the expression stabilities of eight candidate reference genes under different conditions and recommended the optimal suites of reference genes for tissue expression analysis, developmental stages, thermal stress, and pesticide exposure. The findings of this work would facilitate molecular studies of the brassica leaf beetle Phaedon brassicae.
Article
Multidisciplinary Sciences
Meng Wang, Tingting Ren, Prince Marowa, Haina Du, Zongchang Xu
Summary: This study identified stable reference genes PP2A and TUA5 in S. glauca under different salt concentrations and tissues, providing a reliable method for normalizing gene expression. These reference genes were validated in germinating seeds of S. glauca, highlighting their utility in accurate gene expression profiling.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Michihito Deguchi, Shobha Potlakayala, Zachary Spuhler, Hannah George, Vijay Sheri, Ruba Agili, Aayushi Patel, Sairam Rudrabhatla
Summary: Research interest in exploring the pharmaceutical applications of industrial hemp has been significant since its legalization three years ago. This plant, which is mostly dioecious, is known for producing phytocannabinoids, flavonoids, and terpenes. Despite many scientific reports on gene expression analysis of hemp using OMICs approaches, the lack of reliable reference genes for normalizing qRT-PCR data has made it challenging to validate the OMICs data.
Article
Multidisciplinary Sciences
Katherine A. Davies, Stephen R. Welch, Teresa E. Sorvillo, JoAnn D. Coleman-McCray, Maria Laura Martin, Julia M. Brignone, Joel M. Montgomery, Christina F. Spiropoulou, Jessica R. Spengler
Summary: This study investigated the suitability of potential reference genes in laboratory animals commonly used for studying viral hemorrhagic fever infection. The most stable reference gene pairs were identified for each rodent species, and a pan-rodent Ppia assay capable of detecting Ppia across multiple rodent species was developed.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Federica Salatiello, Marco Gerdol, Alberto Pallavicini, Annamaria Locascio, Maria Sirakov
Summary: This study investigated the transcriptome of the bivalve mollusk Mytilus galloprovincialis to identify suitable reference genes for gene expression analyses. Through genome-wide bioinformatics analysis, the Act/Cyp-A gene pair was identified as the most appropriate for gene expression analysis across different tissues.
Article
Biochemical Research Methods
Nirmal Kumar Sampathkumar, Venkat Krishnan SundaramID, Prakroothi S. Danthi, Rasha Barakat, Shiden Solomon, Mrityunjoy Mondal, Ivo Carre, Tatiana El Jalkh, Aida Padilla-Ferrer, Julien Grenier, Charbel Massaad, Jacqueline C. Mitchell
Summary: The choice of reference genes heavily influences the assessment of differential gene expression by qPCR. Recent studies suggest using RNA-Seq to identify stable genes and statistical approaches to determine the best reference genes. However, this study demonstrates that the statistical approach to determine the best reference genes from commonly used candidates is more important than preselecting stable genes from RNA-Seq data.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Physiology
Jinrong Tang, Gemei Liang, Shaoqi Dong, Shuang Shan, Man Zhao, Xianru Guo
Summary: By evaluating the stability of reference genes in Athetis dissimilis under different conditions, suitable reference genes for different experimental conditions can be determined. Different reference genes are needed under different experimental conditions, providing a reliable basis for studying gene expression in A. dissimilis.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Hao Zhan, Hanzhang Liu, Tianchong Wang, Lin Liu, Wanfeng Ai, Xiujun Lu
Summary: This study selected 11 candidate reference genes and evaluated their expression stability in different tissues and under various stress conditions in Quercus mongolica. The results showed that the most stable genes varied in different experimental environments. Moreover, the study demonstrated the reliability of the selected reference genes in different tissues and under different stress conditions.
Article
Food Science & Technology
Nadzirah Amiruddin, Pek-Lan Chan, Kuang-Lim Chan, Pei-Wen Ong, Priscilla Elizabeth Morris, Meilina Ong-Abdullaw, Subhi Siti Masura, Eng-Ti Leslie Low
Summary: A set of reliable reference genes has been identified for accurate measurement and interpretation of gene expression data in oil palm tissues.
JOURNAL OF OIL PALM RESEARCH
(2022)
Article
Multidisciplinary Sciences
Pauline Chatelain, Cecile Blanchard, Jeremy Astier, Agnes Klinguer, David Wendehenne, Sylvain Jeandroz, Claire Rosnoblet
Summary: Microalgae, especially the green alga Klebsormidium nitens, have recently gained attention as important biological models, particularly for studying the transition stages leading to terrestrial life colonization. This study identified optimal reference gene pairs for normalizing RT-qPCR data in K. nitens in response to abiotic stresses. The identified genes also served as marker genes in response to the three tested stresses.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Gothandapani Sellamuthu, Jan Bily, Mallikarjuna Reddy Joga, Jiri Synek, Amit Roy
Summary: This study evaluated the stability of reference genes in Ips typographus under different experimental conditions and identified ribosomal protein 3a (RPS3-a) as the best reference gene. The findings contribute to future genomic and functional genomic research.
SCIENTIFIC REPORTS
(2022)
Article
Cardiac & Cardiovascular Systems
Kei Sato, Nicole White, Jonathon P. Fanning, Nchafatso Obonyo, Michael H. Yamashita, Vinesh Appadurai, Anna Ciullo, Meryta May, Elliott T. Worku, Leticia Helms, Shinichiro Ohshimo, Dafsah A. Juzar, Jacky Y. Suen, Gianluigi Li Bassi, John F. Fraser, Rakesh C. Arora
Summary: This study found that previous use of ACEi/ARBs was associated with a reduced risk of in-hospital mortality in critically ill COVID-19 patients. However, patients in the ACEi/ARB group had longer hospital stay.
BMC CARDIOVASCULAR DISORDERS
(2022)
Article
Critical Care Medicine
Kiran Shekel, Mohd H. Abdul-Aziz, Vesa Cheng, Fay Burrows, Hergen Buscher, Young-Jae Cho, Amanda Corley, Arne Diehl, Eileen Gilder, Stephan M. Jakob, Hyung-Sook Kim, Bianca J. Levkovich, Sung Yoon Lim, Shay McGuinness, Rachael Parke, Vincent Pellegrino, Yok-Ai Que, Claire Reynolds, Sam Rudham, Steven C. Wallis, Susan A. Welch, David Zacharias, John F. Fraser, Jason A. Roberts
Summary: This study aimed to describe the pharmacokinetics of antimicrobials in critically ill patients receiving ECMO and evaluate the effectiveness and safety of current dosing regimens. The study found that there were large variations in antimicrobial concentrations in ECMO patients, leading to poor target attainment rates. Understanding these issues is crucial for the development of appropriate dosing regimens.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Immunology
Arutha Kulasinghe, Ning Liu, Chin Wee Tan, James Monkman, Jane E. Sinclair, Dharmesh D. Bhuva, David Godbolt, Liuliu Pan, Andy Nam, Habib Sadeghirad, Kei Sato, Gianluigi Li Bassi, Ken O'Byrne, Camila Hartmann, Anna Flavia Ribeiro Dos Santos Miggiolaro, Gustavo Lenci Marques, Lidia Zytynski Moura, Derek Richard, Mark Adams, Lucia de Noronha, Cristina Pellegrino Baena, Jacky Y. Suen, Rakesh Arora, Gabrielle T. Belz, Kirsty R. Short, Melissa J. Davis, Fernando Souza-Fonseca Guimaraes, John F. Fraser
Summary: The study reveals distinct transcriptomic profiles in cardiac tissues of SARS-CoV-2 and pH1N1 influenza infection, with upregulation of genes associated with DNA damage and repair, heat shock, and macrophage infiltration in COVID-19 patients' cardiac tissues. In comparison, pH1N1 infection showed upregulation of interferon and complement pathways. This highlights the need for further understanding of the effects on extra-pulmonary organs, including the cardiovascular system, in COVID-19 patients.
Article
Engineering, Biomedical
Peipei Jing, Meili Zhang, Chris H. H. Chan, Fengjuan Jing, Jo P. Pauls, Matthew S. Dargusch, John F. Fraser, Yongxiang Leng
Summary: In order to improve the poor tribological properties of titanium and its alloy, diamond-like carbon (DLC) films are pursued to enhance the wear resistance. DLC films are preferred to be prepared at low temperature considering the effect of temperature on magnets and workpiece deformation. This study prepared DLC films on Ti6Al4V alloys using periodic and continuous processes, and compared their properties. Both DLC films showed reduced friction coefficient and wear rate, and the periodic DLC films exhibited superior anti-wear properties in fetal bovine serum. The study demonstrates the potential of low-temperature DLC films as wear-resistant surface modification for ventricular assist devices (VADs).
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Immunology
Maryam Shojaei, Amir Shamshirian, James Monkman, Laura Grice, Minh Tran, Chin Wee Tan, Siok Min Teo, Gustavo Rodrigues Rossi, Timothy R. McCulloch, Marek Nalos, Maedeh Raei, Alireza Razavi, Roya Ghasemian, Mobina Gheibi, Fatemeh Roozbeh, Peter D. Sly, Kirsten M. Spann, Keng Yih Chew, Yanshan Zhu, Yao Xia, Timothy J. Wells, Alexandra Cristina Senegaglia, Carmen Lucia Kuniyoshi, Claudio Luciano Franck, Anna Flavia Ribeiro dos Santos, Lucia de Noronha, Sepideh Motamen, Reza Valadan, Omolbanin Amjadi, Rajan Gogna, Esha Madan, Reza Alizadeh-Navaei, Liliana Lamperti, Felipe Zuniga, Estefania Nova-Lamperti, Gonzalo Labarca, Ben Knippenberg, Velma Herwanto, Ya Wang, Amy Phu, Tracy Chew, Timothy Kwan, Karan Kim, Sally Teoh, Tiana M. Pelaia, Win Sen Kuan, Yvette Jee, Jon Iredell, Ken O'Byrne, John F. Fraser, Melissa J. Davis, Gabrielle T. Belz, Majid E. Warkiani, Carlos Salomon Gallo, Fernando Souza-Fonseca-Guimaraes, Quan Nguyen, Anthony Mclean, Arutha Kulasinghe, Kirsty R. Short, Benjamin Tang
Summary: This study found that the IFI27 gene is associated with COVID-19 infection and that its expression levels in the respiratory tract and blood are correlated with viral load and infection severity. IFI27 gene family biomarkers may supplement conventional diagnostic tools in future virus pandemics.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Biomedical
Silver Heinsar, Nicole Bartnikowski, Gunter Hartel, Samia M. Farah, E-Peng Seah, Eric Wu, Sebastiano Maria Colombo, Clayton Semenzin, Andrew Haymet, Indrek Ratsep, Jo Pauls, John F. Fraser, Jacky Y. Suen
Summary: This study comprehensively assessed different parameters of pulsatile-flow veno-arterial extracorporeal membrane oxygenation (V-A ECMO) and their contribution to hemodynamic energy production and transfer. The results showed that increasing flow rates and pulsatile amplitudes were associated with higher hemodynamic energy production, while adjusting systole-to-diastole ratio or pulsing frequency had no significant effect. Arterial cannula was found to be the main source of resistance to hemodynamic energy transfer. Increased flow rate and amplitude were the key factors for hemodynamic energy production, while other factors were relevant when combined.
Article
Engineering, Biomedical
Fung Ming Lau, Wai Kit Chan, Yuen Tin Mok, Peter Chi Keung Lai, Sin Kwan Tammy Ma, Chun Wai Ngai, Wai Ching Sin, Wai Ling Phyllis Kwok, Kin Yip Yu, Wai Ming Chan, John F. Fraser, Pauline Yeung Ng
Summary: PCRTO is a weaning technique that involves decremental pump revolutions to achieve retrograde flow, and its application in adults has not been widely reported.
Review
Engineering, Biomedical
Dhayananth Kanagarajan, Silver Heinsar, Lucia Gandini, Jacky Y. Suen, Van Thanh Dau, Jo Pauls, John F. Fraser
Summary: Refractory cardiogenic shock is currently treated with veno-arterial extracorporeal membrane oxygenation (V-A ECMO) without conclusive evidence of improved clinical outcomes. Pulsatile V-A ECMO has been developed as a potential solution to the limitations of continuous-flow devices. A systematic review of preclinical studies on pulsatile V-A ECMO revealed that most studies focused on hemodynamic energy production, while the clinical effects such as heart and brain function, end-organ microcirculation, and inflammation reduction are still inconclusive.
Article
Critical Care Medicine
Jonathon Fanning, Natasha B. Weaver, Robert J. Fanning, Matthew Griffee, Sung-Min Cho, Mauro G. Panigada, Nchafatso M. Obonyo, Akram Zaaqoq, Hannah Rando, Yew Woon Chia, Bingwen Eugene Fan, Declan Sela, Davide Chiumello, Silvia Coppola, Ahmed Labib, Glenn J. R. C. Whitman, Rakesh S. Arora, Bo Kim, Anna Motos, Antoni Torres, Ferran Barbe, Giacomo Grasselli, Alberto Zanella, Eric Etchill, Asad Ali Usman, Maximilian M. Feth, Nicole Y. White, Jacky Suen, Gianluigi J. Li Bassi, Giles F. Peek, John Fraser, Heidi Dalton
Summary: A high prevalence of HECTOR complications (hemorrhage, disseminated intravascular coagulopathy, and thrombosis) was observed in ICU patients with severe COVID-19. Hemorrhagic complications were associated with increased ICU mortality, while thrombosis complications were associated with reduced mortality.
CRITICAL CARE MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Hayato Taniguchi, Indrek Ratsep, Silver Heinsar, Keibun Liu, Marcela Cespedes, Jacky Y. Suen, Gianluigi Li Bassi, John F. Fraser, Jeffrey P. Jacobs, Giles J. Peek
Summary: Iliopsoas haematoma (IPH) is a rare bleeding complication during extracorporeal membrane oxygenation (ECMO) with unclear incidence and risk factors. This study used an international multi-centre cohort registry and found an overall incidence rate of 0.64% for IPH in COVID-19 ECMO patients, with comparable rates between countries. The IPH group had a higher mortality rate (71.4%) compared to the non-IPH group. Increased awareness about IPH occurrence is important due to its high fatality rate.
Review
Cardiac & Cardiovascular Systems
Jeffrey Javidfar, Akram M. Zaaqoq, Ahmed Labib, Adrian G. Barnett, J. W. Awori Hayanga, Greg Eschun, Michael H. Yamashita, Jeffrey P. Jacobs, Silver Heinsar, Jacky Y. Suen, John F. Fraser, Gianluigi Li Bassi, Rakesh C. Arora, Giles J. Peek
Summary: Obesity is linked to a worse prognosis in COVID-19 patients with ARDS. It remains unclear how morbid obesity directly affects patients treated with V-V ECMO.
Meeting Abstract
Hematology
Tara Quinton, John Fraser, Karen M. Black, Ali Sadeghi-Khomami
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Meeting Abstract
Critical Care Medicine
Chengda Zhang, Bishoy Zakhary, Nicole White, Silver Heinsar, Aidan Burrell, Akram Zaaqoq, Jeffrey Jacobs, Heidi Dalton, Ahmed Labib, Jacky Suen, Silvia Mariani, Gianluigi Li Bassi, John Fraser, Giles Peek
CRITICAL CARE MEDICINE
(2023)
Article
Critical Care Medicine
Bairbre A. A. McNicholas, Emanuele Rezoagli, Andrew J. J. Simpkin, Sankalp Khanna, Jacky Y. Y. Suen, Pauline Yeung, Daniel Brodie, Gianluigi Li Bassi, Tai Pham, Giacomo Bellani, John F. F. Fraser, John Laffey
Summary: AKI is a common and serious complication in COVID-19 patients, with different characteristics and outcomes compared to non-COVID-19 ARDS patients.
Article
Critical Care Medicine
John P. R. Moore, Yahya Shehabi, Michael C. Reade, Michael Bailey, John F. Fraser, Lauren Murray, Christopher Anstey, Mervyn Singer
Summary: This study aimed to compare the impact of early sedation with dexmedetomidine and usual sedatives on the stress response in critically ill patients on mechanical ventilation. The results showed that early sedation with dexmedetomidine had comparable effects on physiological and blood-borne parameters associated with the stress response compared to usual-care sedation.