Article
Biochemical Research Methods
Jiekai Yin, Tianyu F. Qi, Yen-Yu Yang, Madeline Vera-Colon, Nicole I. zur Nieden, Yinsheng Wang
Summary: RNA modifications and their associated proteins play a regulatory role in osteogenesis, and this study provides comprehensive quantitative assessment of these proteins during osteogenic differentiation. The down-regulation of certain RNA modification proteins, mainly involved in methylation and pseudouridylation, was found. Protein-protein interaction analysis revealed their significant associations with osteogenesis-related proteins, and gene set enrichment analysis suggested a potential role of METTL1 in osteogenesis through the cytokine network.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Immunology
Chuanfeng Wu, Jialiu A. Liang, Jason M. Brenchley, Taehoon Shin, Xing Fan, Ryland D. Mortlock, Diana M. Abraham, David S. J. Allan, Marvin L. Thomas, So Gun Hong, Cynthia E. Dunbar
Summary: Tissue resident immune cells, including T cells, B cells, myeloid cells, and NK cells, have distinct clonal distribution patterns across different tissues. The clonal expansion of TR NK cells varies between individual animals, and specific TR NK clones are found in different tissues. These findings suggest the importance of studying NK cell tissue immunological memory for the development of NK based immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Ryland D. Mortlock, Chuanfeng Wu, E. Lake Potter, Diana M. Abraham, David S. J. Allan, So Gun Hong, Mario Roederer, Cynthia E. Dunbar
Summary: The distribution and trafficking patterns of natural killer (NK) cells in vivo remain insufficiently studied. This study utilized rhesus macaque (RM) primates as animal models to investigate the tissue distribution and localization patterns of three NK cell subsets. The study found that CD16+ NK cells were selectively retained in the vasculature at steady state, while CD56+ NK cells had a shorter residence time in peripheral blood. Different subsets of NK cells also showed distinct trafficking kinetics to and from lymph nodes and other tissues. Furthermore, following administration of CD16-depleting antibody, CD16+ NK cells and their precursors retained a high proportion of continuously circulating cells, suggesting regeneration of the CD16 NK compartment may occur in peripheral blood or the perivascular compartments of tissues.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Mohammad Zahidul Hasan, Charlotte Hoeltermann, Beatrix Petersen, Annette Schrod, Kerstin Maetz-Rensing, Artur Kaul, Gabriela Salinas, Ralf Dressel, Lutz Walter
Summary: This study used newly established monoclonal antibodies to detect the expression of NKG2C receptors on primary NK cells in rhesus macaques. The results showed that NKG2C receptor expression was higher in CMV-positive macaques. Furthermore, these NKG2C-positive NK cells had specific molecular features and exhibited high expression of IFNG, indicating their association with CMV.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Xin Li, Haiguo Jin, Yang Lv, Chen Liu, Xiaotong Luo, Jianqiang Liu, Qi Zhang, Yongsheng Yu, Zhongli Zhao
Summary: This study investigated the differentially expressed miRNAs during the differentiation of chicken embryonic stem cells into male germ cells. A total of 113 differentially expressed miRNAs were identified, and their target genes were enriched in various signaling pathways. The study also demonstrated the regulation between miR-383-5p and its target gene STRN3 and constructed corresponding gene reporter vectors.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jing Cao, Wenjuan Li, Jie Li, Md. Abdul Mazid, Chunyang Li, Yu Jiang, Wenqi Jia, Liang Wu, Zhaodi Liao, Shiyu Sun, Weixiang Song, Jiqiang Fu, Yan Wang, Yong Lu, Yuting Xu, Yanhong Nie, Xinyan Bian, Changshan Gao, Xiaotong Zhang, Liansheng Zhang, Shenshen Shang, Yunpan Li, Lixin Fu, Hao Liu, Junjian Lai, Yang Wang, Yue Yuan, Xin Jin, Yan Li, Chuanyu Liu, Yiwei Lai, Xuyang Shi, Patrick H. Maxwell, Xun Xu, Longqi Liu, Muming Poo, Xiaolong Wang, Qiang Sun, Miguel A. Esteban, Zhen Liu
Summary: By optimizing the culture conditions for monkey naive embryonic stem cells, researchers have successfully generated live chimeric monkeys with high donor cell contribution. This finding has significant implications for the study of primate naive pluripotency and genetic engineering of non-human primates.
Article
Zoology
Qiang Ma, Wenji Ma, Tian-Zhang Song, Zhaobo Wu, Zeyuan Liu, Zhenxiang Hu, Jian-Bao Han, Ling Xu, Bo Zeng, Bosong Wang, Yinuo Sun, Dan-Dan Yu, Qian Wu, Yong-Gang Yao, Yong-Tang Zheng, Xiaoqun Wang
Summary: This study analyzed single-nucleus transcriptomes from multiple organs of SARS-CoV-2-infected rhesus macaques, revealing that the liver had the most significant global transcriptional alterations. Lung epithelial cells, especially AT2 and ciliated cells, showed significant impairment, while fibroblasts exhibited signs of fibrosis. The kidney dataset indicated a tropism of tubule cells to SARS-CoV-2 infection, leading to membranous nephropathy. Pathological changes were also observed in astrocytes and oligodendrocytes in the cerebral cortex. Overall, this study broadened our understanding of disease features and immune defects caused by SARS-CoV-2 infection.
ZOOLOGICAL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Ye Zhang, Yidi Ma, Juan Chen, Min Wang, Yuan Cao, Lei Li, Hua Yang, Xudong Liu, Yaqian Li, Lan Zhu
Summary: The study evaluated the potential of mesenchymal stem cell (MSC) therapy in repairing weak vaginal tissue in an ovariectomized rhesus macaque model. Results showed that MSC transplantation could promote extracellular matrix ingrowth, neovascularization, and smooth muscle formation, ultimately improving the biomechanical properties of the vagina. This highlights a new prospective treatment for pelvic organ prolapse (POP).
STEM CELL RESEARCH & THERAPY
(2021)
Article
Environmental Sciences
Jianming Wang, Tiantian Liu, Jin Wang, Tao Chen, Yan Jiang
Summary: This study observed the effects of PM2.5 on human embryonic stem cells and found that it interferes with DNA methylation and mRNA expression, leading to aberrant cell proliferation. Additionally, PM2.5 significantly influenced the transcriptome patterns and DNA methylome of the cells. These findings provide new insights into the molecular mechanisms underlying the developmental toxicity of PM2.5.
Article
Biochemistry & Molecular Biology
Ambra Costa, Davide Ceresa, Antonella De Palma, Rossana Rossi, Sara Turturo, Sara Santamaria, Carolina Balbi, Federico Villa, Daniele Reverberi, Katia Cortese, Pierangela De Biasio, Dario Paladini, Domenico Coviello, Silvia Ravera, Paolo Malatesta, Pierluigi Mauri, Rodolfo Quarto, Sveva Bollini
Summary: This study provides a detailed characterization of the total secretome and extracellular vesicles of human amniotic-fluid derived stem cells (hAFS), comparing fetal and perinatal cells. Both sources of cells offer formulations enriched with factors promoting regeneration, with fetal hAFS showing a more pronounced pro-vasculogenic and anti-aging profile. The study confirms that hAFS represent a promising source of regenerative paracrine factors for potential therapy consideration based on specific clinical scenarios.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Jessica T. Jacobs, Rafael S. Maior, Hannah F. Waguespack, Carolina Campos-Rodriguez, Patrick A. Forcelli, Ludise Malkova
Summary: The bed nucleus of the stria terminalis (BNST) is involved in various social behaviors, including aggression, maternal care, mating behavior, and social interaction. Activation of the BNST has been shown to reduce social interaction between unfamiliar animals in rodent studies. However, the role of the BNST in social interaction in primates has not been investigated. By temporarily inactivating the BNST in male macaque monkeys, we found that BNST inactivation led to a significant increase in social interaction, accompanied by an increase in passive contact and a significant decrease in locomotion.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Immunology
Brianna C. Davey, Mary S. Pampusch, Emily K. Cartwright, Hadia M. Abdelaal, Eva G. Rakasz, Aaron Rendahl, Edward A. Berger, Pamela J. Skinner
Summary: T cells expressing a SIV-specific chimeric antigen receptor (CAR) and CXCR5 were infused into SIV-infected rhesus macaques on antiretroviral therapy (ART) to assess their ability to control the virus. These cells showed evidence of functionality but failed to persist in the animals beyond 28 days, possibly due to the development of anti-CAR antibodies. The study suggests that the CD4 and CD28 domains of the CAR are immunogenic and could impact the long-term persistence of CAR immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hong Gao, Tie Zhang, Meng-Meng Lun, Jun-Yi Li, Hai-Feng Lu, Da-Wei Fu, Yi Zhang
Summary: This study presents a halogen regulation design of organic molecular strategy to enhance the multifunctional properties of organic-inorganic hybrid phase transition materials. The results show improved SHG response and dielectric phase transition temperature, as well as efficient green light emission under UV irradiation. This work is significant for exploring multifunctional molecular switch materials through halogen modification strategies.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Ying-Ying Gong, Tie Zhang, Jie Li, Da-Wei Fu, Yi Zhang, Hai-Feng Lu
Summary: Low-dimensional hybrid materials show greater structural flexibility and adjustability compared to high-dimensional materials. The effect of halogen substitution on low-dimensional materials was investigated, leading to changes in photoelectric properties. Therefore, this strategy allows for the achievement of multiple properties in materials.
CRYSTAL GROWTH & DESIGN
(2022)
Article
Electrochemistry
Fuchun Si, Yuzhi Sun, Yanyan Ba, Liang Guo, Yanju Liu, Jinming Kong
Summary: A convenient electrochemiluminescence (ECL) biosensor was developed using a metal-free photoinduced atom transfer radical polymerization (photo-ATRP) strategy for sensitive detection of CYFRA 21-1. The biosensor demonstrated high selectivity, low detection limit, specific selectivity, and excellent stability and reproducibility.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Environmental Sciences
Huabin Wang, Yi Wen, Yu Ding, Zhiqiang Yue, Dan Xu, Ying Liu, Yong Zhang, Rui Xu, Weiqing Zeng
Summary: Cow manure derived biochar (CMBC) is a promising functional material that has potential applications as an ecofriendly approach. This study revealed the significant role of released phosphorus from CMBC in Pb(II) removal and its ability to immobilize Pb(II) in soil and promote plant growth.
Article
Environmental Sciences
Jiwei Xu, Lumeng Li, Huabin Wang, Zhanyuan Gao, Chuanshu Wang, Rong Sun, Yong Zhang, Wumei Xu, Xiying Hou, Rui Xu
Summary: The indigenous chromium-resistant Aspergillus niger strain (A1) was successfully isolated and screened from heavily chromium-contaminated red soil in Yunnan Province, China. The strain exhibited strong tolerance to toxic chromium and showed efficient adsorption of chromium through intracellular metabolism, surface complexations with EPS, and chemical reduction. Inoculation of A1 into chromium-contaminated red soil resulted in a significant reduction in Cr6+ content and a shift from high-toxicity chromium state to low-toxicity chromium state.
Article
Biophysics
Bing Li, Yunxiao Li, Chenchen Li, Jinghui Yang, Deling Liu, Huabin Wang, Rui Xu, Yong Zhang, Qin Wei
Summary: In this study, a novel split-type electrochemical immunosensor based on controlled release strategy was proposed for sensitive analysis and detection of tumor marker carbohydrate antigen 199 (CA19-9). The immunosensor showed wide linear concentration range and highly sensitive detection of CA19-9. It provides an innovative approach for trace detection of other biomarkers.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Genetics & Heredity
Jiang Liu, Yan-Feng Zhang, Zhu-Mei Ren
Summary: The complete mitogenome of the Rhus gall aphid Schlechtendalia peitan in China was obtained using the shotgun genome-skimming method on an Illumina platform. It has a length of 15,494 bp and a high A + T content of 84.1%. The mitogenome consists of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region.
CYTOLOGY AND GENETICS
(2023)
Article
Chemistry, Analytical
Zhengfang Qu, Yunxiao Li, Rui Xu, Chenchen Li, Huan Wang, Huabin Wang, Yong Zhang, Qin Wei
Summary: A candy-like WO3/Fe2O3 nanocomposite with excellent performance was prepared by modifying the content of Fe element in the n-n heterojunction WO3/Fe2O3 nanocomposites. The candy-like nanocomposite exhibited better selectivity and sensitivity for sensing triethylamine gases at a lower operating temperature (260 °C) compared with the gas sensor prepared with WO3 alone. The feasibility was verified through investigation of the sensing mechanism and real sample tests. A TEA gas sensor with low limit of detection, short response/recovery time (15/162 s), and high sensitivity was successfully developed, demonstrating good stability, selectivity, and practical application value.
Article
Chemistry, Analytical
Yingying Wang, Huixin Wang, Yaliang Bai, Guanhui Zhao, Nuo Zhang, Yong Zhang, Yaoguang Wang, Hong Chi
Summary: In this study, an electrochemical immunoassay method based on molybdenum disulfide (MoS2) was proposed to detect neuron-specific enolase (NSE) sensitively. The MoS2@Au nanozyme, formed by composite MoS2 and Au nanoclusters (Au NCs), was used to catalyze the decomposition of H2O2, which amplified the amperometric response and improved sensitivity. The detection range of this strategy was 0.1 pg·mL(-1)-10 ng·mL(-1) with a limit of detection of 0.05 pg·mL(-1). This sensing strategy showed potential for sensitive detection of NSE and can provide a theoretical basis and technical support for detecting other disease markers.
Article
Agricultural Engineering
Huabin Wang, Dingxiang Chen, Ting Cui, Ran Duan, Xianghong Yan, Yong Zhang, Rui Xu
Summary: In this study, a bifunctional zero-valent iron-modified hydrochar derived from walnut peel (MWPHC) was successfully synthesized and applied for the simultaneous removal of tetracycline and copper from wastewater. Fe0 species were distributed on the surface of the walnut peel substrates. MWPHC exhibited excellent performance in the simultaneous removal of tetracycline and copper, and bridging effects were observed between them. The feasibility of MWPHC was evaluated through column and regeneration experiments, and it showed great potential as an environmental functional material for pollution control of co-existing antibiotic and heavy metal contaminants in agro-industrial wastewater.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Chemical
Yi Wen, Dingxiang Chen, Yong Zhang, Huabin Wang, Rui Xu
Summary: Designing an efficient and recyclable adsorbent for cadmium pollution control is an urgent necessity. In this study, magnetic cow manure biochar was synthesized using cow manure as the raw material. The optimal preparation conditions and reaction conditions were determined through a response surface methodology model. The adsorption mechanism involved ion exchange, electrostatic attraction, pore-filling, co-precipitation, and the formation of complexations.
Meeting Abstract
Oncology
Chang-Ching Lin, Tsung-Cheng Chang, Yunguan Wang, Yanfeng Zhang, Andrew Lemoff, Yisheng V. Fang, He Zhang, Dan Ye, Isabel Soria-Bretones, Alberto Servetto, Kyung-Min Lee, Xuemei Luo, Joseph J. Otto, Hiroaki Akamatsu, David W. Cescon, Lin Xu, Yang Xie, Joshua T. Mendell, Ariella B. Hanker, Carlos L. Arteaga
Article
Chemistry, Multidisciplinary
Zhao-Bo Hu, Long-He Li, Yibo Han, Jinlei Zhang, Jianrong Li, Zilu Chen, Shuyi Wu, Yi Zhang, Heng-Yun Ye, You Song
Summary: Magnet-optical materials have potential applications in microelectronics and optoelectronic devices. However, most research focuses on inorganic materials, and there have been few reports on molecule materials. This study synthesized an organic-inorganic hybrid complex with perovskite structure, which showed unique magneto-optical coupling and optical hysteresis effects.
Article
Chemistry, Analytical
Yunxiao Li, Jiwei Xu, Rongqi Cheng, Jinghui Yang, ChenChen Li, Yingchun Liu, Rui Xu, Qin Wei, Yong Zhang
Summary: A molecularly imprinted electrochemiluminescence sensor was developed for the selective and sensitive detection of spiramycin. The sensor showed good selectivity and sensitivity by combining molecularly imprinted polymer and electrochemiluminescence techniques, and enhancing the electrochemiluminescence signal through modification of nanoarrays.
Article
Chemistry, Inorganic & Nuclear
Zhi-Cheng Zhang, Tie Zhang, Pei-Zhi Huang, Ting Shao, Da-Wei Fu, Yi Zhang
Summary: Organic-inorganic hybrid materials have been extensively studied for their superior properties and potential applications in various fields. We have developed two compounds with transformable double channels of nonlinear optical and dielectric properties induced by structural phase transitions, which can be switched ON and OFF by temperature regulation. This work provides inspiration and options for the exploration of opto-electrical dual channel components.
DALTON TRANSACTIONS
(2022)
