Article
Agriculture, Multidisciplinary
Paloma Delgado-Fernandez, Blanca de las Rivas, Rosario Munoz, Maria Luisa Jimeno, Elisa G. Doyaguez, Nieves Corzo, F. Javier Moreno
Summary: This study highlights the high efficiency of MelA alpha-galactosidase in transferring galactosyl residues from melibiose to produce novel galactose-containing hetero-oligosaccharides. The synthesized non-digestible alpha-galactosides have potential applications in selectively modulating gut microbiota composition.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Microbiology
Anh-Minh Tran, Kridsada Unban, Apinun Kanpiengjai, Chartchai Khanongnuch, Geir Mathiesen, Dietmar Haltrich, Thu-Ha Nguyen
Summary: Lactiplantibacillus plantarum was utilized to produce a food-grade lactobacillal alpha-amylase, with the best signal peptide identified as Lp_2145 resulting in a significant increase in mRNA levels and efficient extracellular enzymatic activities.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Senlin Li, Lina Guo, Xuemeng Si, Zhaolai Dai, Zhigang Zhou, Zhenlong Wu
Summary: This study found that Lactobacillus plantarum WCFS1 has a protective effect on zebrafish against Aeromonas hydrophila infection by reducing damage and inflammation. It regulates autophagy and hypoxia-inducible factors, as well as inhibits the expression of muscle atrophy-related genes, thereby exerting its antibacterial effects.
Article
Biochemistry & Molecular Biology
Alvaro Ferreira-Lazarte, Laura Plaza-Vinuesa, Blanca de las Rivas, Mar Villamiel, Rosario Munoz, F. Javier Moreno
Summary: This study investigated the amino acid sequence, structural analysis, biochemical properties, and glycosidase activity of two recombinant alpha-rhamnosidases, Ram1 and Ram2, from Lactobacillus plantarum WCFS1. Ram2 demonstrated a substrate specificity towards alpha-(1→6) glycosidic flavonoids and potential cost-effective production of isoquercetin. PPO was found to efficiently convert naringin and rutin into their corresponding aglycones, suggesting its potential use for improving the sensory properties of beverages.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Xiaoxu Luan, Meiqin Feng, Jian Sun
Summary: The study explored the in vitro antioxidant activity of Lactobacillus plantarum CD101 and its impact on antioxidant peptide formation in fermented sausage. L. plantarum CD101 mainly distributed antioxidant substances on the cell surface and extracellular secretions. The potential benefit of L. plantarum CD101 in inhibiting oxidative rancidity and promoting protein degradation was observed, with a weak effect on promoting antioxidant peptides.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
E. M. J. Vaessen, H. A. Kemme, R. A. H. Timmermans, M. A. I. Schutyser, H. M. W. den Besten
Summary: This study demonstrates that the suitable temperature and optimized medium are crucial for the survival and molecule uptake of microbial cells in pulsed electric field processing, which can enhance cell robustness and trehalose concentration.
BIOELECTROCHEMISTRY
(2021)
Article
Food Science & Technology
Li-Long Pan, Ming Zhang, Zhengying Li, Binbin Li, Xiaohua Pan, Xiaopei Chen, Bo Yang, Hao Zhang, Wei Chen, Le Zhang, Jia Sun
Summary: In this study, engineered probiotics encoding CRAMP were shown to increase colonic CRAMP production, reduce colon shortening and weight loss, and suppress inflammatory responses in experimental colitis. These findings suggest that CRAMP-encoding L. plantarum may have therapeutic potential for colitis through modulation of colonic CRAMP production.
Article
Engineering, Chemical
I Siemons, E. M. J. Vaessen, S. E. Oosterbaan van Peski, R. M. Boom, M. A. Schutyser
Summary: This study used single droplet drying to investigate the relationship between particle morphology and survival of Lactobacillus plantarum WCFS1. The research showed a clear correlation between particle morphologies and viabilities after drying, with dense and smooth particles exhibiting the highest survival rates. The identified correlation between morphology development and bacterial survival will be important in understanding the mechanisms of inactivation during drying.
JOURNAL OF FOOD ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Binbin Chen, Bryan Zong Lin Loo, Ying Ying Cheng, Peng Song, Huan Fan, Oleg Latypov, Sandra Kittelmann
Summary: Proteases play a crucial role in improving protein digestibility, nutrient availability, and flavor of fermented food and feed products. This study optimized protease expression and secretion in L. plantarum and P. acidilactici through genetic engineering. A versatile plasmid and a screening of signal peptides led to the identification of novel peptides that enhanced protease secretion.
Article
Biochemistry & Molecular Biology
Eduardo Rosado, Paloma Delgado-Fernandez, Blanca de las Rivas, Rosario Munoz, Francisco Javier Moreno, Nieves Corzo, Cesar Mateo
Summary: This study presents the synthesis of beta-galactosyl xylitol derivatives using immobilized LacA beta-galactosidase from Lactobacillus plantarum WCFS1. The immobilized enzyme exhibited high activity and stability, allowing for the synthesis of a large amount of beta-galactosyl xylitol derivatives with improved efficiency. It also showed lower in vitro intestinal digestibility compared to lactose.
Article
Immunology
Qingxue Chen, Song Wang, Jiayao Guo, Qinggang Xie, Smith Etareri Evivie, Yue Song, Bailiang Li, Guicheng Huo
Summary: The study demonstrated the alleviative effects of L. plantarum KLDS 1.0344 in treating mastitis. In vitro experiments showed its antibacterial properties against E. coli, while in vivo studies indicated a reduction in inflammatory factors expression and prevention of inflammation through inhibiting the NF-κB signaling pathway activation. This strain shows potential as an alternative to antibiotics for preventing mastitis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Lina Zhao, Qinggang Xie, Fengyi Shi, Shengnan Liang, Qingxue Chen, Smith Etareri Evivie, Ji Qiu, Bailiang Li, Guicheng Huo
Summary: This study aimed to reduce cow milk protein allergenicity by fermenting skim milk protein with Lactobacillus helveticus and Lactobacillus plantarum strains. The fermentation process inhibited antigenicity of specific milk proteins and cleaved major allergic epitopes, demonstrating a potential method for reducing milk sensitization.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Li Chen, Yixin Hui, Tingting Gao, Guowei Shu, He Chen
Summary: This study isolated a novel wild Lactobacillus plantarum 60 with strong antioxidant activity, obtained three novel antioxidative peptides from goat milk fermented with L60, and showed significantly increased antioxidant capacities in purified peptides applied in goat milk powder.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Yan-yan Huang, Dong-mei Liu, Xiang-ze Jia, Ming-hua Liang, Yongzhi Lu, Jinsong Liu
Summary: The study found that LP9010 has a certain tolerance to nitrite, but high concentrations of nitrite inhibit its growth and acid production. Nitrite not only affects the morphology and surface characteristics of LP9010 cells, but also influences genes related to stress proteins and cell wall synthesis in the genome. This research provides theoretical reference for reducing nitrite in food products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jinkui Sun, Cai Zhao, Xilei Pu, Tian Li, Xuewei Shi, Bin Wang, Weidong Cheng
Summary: This study demonstrates that fermentation with Lactobacillus plantarum LP56 can enhance the nutritional value and aroma characteristics of apricot juice. Fermented apricot juice contains more probiotics and lactic acid, with increased antioxidant capacity and decreased levels of organic acids and soluble solids.
FERMENTATION-BASEL
(2022)
Article
Oncology
Ina A. Eilertsen, Anita Sveen, Jonas M. Stromme, Rolf I. Skotheim, Arild Nesbakken, Ragnhild A. Lothe
INTERNATIONAL JOURNAL OF CANCER
(2019)
Review
Oncology
Anita Sveen, Scott Kopetz, Ragnhild A. Lothe
NATURE REVIEWS CLINICAL ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Kaja C. G. Berg, Anita Sveen, Maren Holand, Sharmini Alagaratnam, Marianne Berg, Stine A. Danielsen, Arild Nesbakken, Kjetil Soreide, Ragnhild A. Lothel
Article
Oncology
R. Dienstmann, G. Villacampa, A. Sveen, M. J. Mason, D. Niedzwiecki, A. Nesbakken, V. Moreno, R. S. Warren, R. A. Lothe, J. Guinney
ANNALS OF ONCOLOGY
(2019)
Editorial Material
Oncology
A. Sveen, C. Cremolini, R. Dienstmann
ANNALS OF ONCOLOGY
(2019)
Article
Oncology
Ina A. Eilertsen, Seyed H. Moosavi, Jonas M. Stromme, Arild Nesbakken, Bjarne Johannessen, Ragnhild A. Lothe, Anita Sveen
Article
Oncology
Tuva Host Brunsell, Anita Sveen, Bjorn Atle Bjornbeth, Bard Rosok, Stine Aske Danielsen, Kristoffer Watten Brudvik, Kaja C. G. Berg, Bjarne Johannessen, Vanja Cengija, Andreas Abildgaard, Marianne Gronlie Guren, Arild Nesbakken, Ragnhild A. Lothe
CLINICAL COLORECTAL CANCER
(2020)
Article
Oncology
Jarle Bruun, Kushtrim Kryeziu, Peter W. Eide, Seyed H. Moosavi, Ina A. Eilertsen, Jonas Langerud, Bard Rosok, Max Z. Totland, Tuva H. Brunsell, Teijo Pellinen, Jani Saarela, Christian H. Bergsland, Hector G. Palmer, Kristoffer W. Brudvik, Tormod Guren, Rodrigo Dienstmann, Marianne G. Guren, Arild Nesbakken, Bjorn Atle Bjornbeth, Anita Sveen, Ragnhild A. Lothe
CLINICAL CANCER RESEARCH
(2020)
Article
Oncology
Marine Jeanmougin, Annette B. Havik, Lina Cekaite, Petter Brandal, Anita Sveen, Torstein R. Meling, Trude H. Agesen, David Scheie, Sverre Heim, Ragnhild A. Lothe, Guro E. Lind
MOLECULAR ONCOLOGY
(2020)
Article
Hematology
Jillian F. Wise, Sigve Nakken, Chloe B. Steen, Daniel Vodak, Gunhild Troen, Bjarne Johannessen, Ole Christian Lingjxrde, Vera Hilden, Yngvild Nuvin Blaker, Baoyan Bai, Lars Birger Aasheim, Annika Pasanen, Susanne Lorenz, Anita Sveen, Ragnhild A. Lothe, Ola Myklebost, Sirpa Leppa, Leonardo A. Meza-Zepeda, Klaus Beiske, Michael S. Lawrence, Eivind Hovig, June Helen Myklebust, Erlend B. Smeland, Harald Holte
Article
Medicine, General & Internal
Jorgen Smeby, Kushtrim Kryeziu, Kaja C. G. Berg, Ina A. Eilertsen, Peter W. Eide, Bjarne Johannessen, Marianne G. Guren, Arild Nesbakken, Jarle Bruun, Ragnhild A. Lothe, Anita Sveen
Article
Oncology
Bjornar T. B. Flatin, Hege Marie Vedeld, Rita Pinto, Jonas Langerud, Guro E. Lind, Ragnhild A. Lothe, Anita Sveen, Marine Jeanmougin
Summary: Intratumor heterogeneity of colorectal cancers (CRCs) is mainly reflected in the genomic and epigenomic levels. Our study revealed that CIMP status in primary CRCs is consistent, although spatial discrepancies in individual genes indicate the potential of large-scale analysis of multiregional samples to identify robust CIMP markers for intratumor heterogeneity.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Biotechnology & Applied Microbiology
Jonas M. Stromme, Bjarne Johannessen, Susanne G. Kidd, Mari Bogaard, Kristina T. Carm, Xiaokang Zhang, Anita Sveen, Anthony Mathelier, Ragnhild A. Lothe, Ulrika Axcrona, Karol Axcrona, Rolf I. Skotheim
Summary: The study revealed extensive heterogeneity at the gene expression level in multifocal prostate cancer. Different malignant foci within the same patient showed similar levels of heterogeneity as tumors from different patients. Expression patterns linked to ETS status and extraprostatic extension were identified, and a set of 62 genes with low intrapatient heterogeneity and high interpatient heterogeneity were found, which could serve as prognostic biomarkers regardless of the sampled malignant focus.
CANCER GENE THERAPY
(2022)
Article
Pathology
Christian H. Bergsland, Marine Jeanmougin, Seyed H. Moosavi, Aud Svindland, Jarle Bruun, Arild Nesbakken, Anita Sveen, Ragnhild A. Lothe
Summary: This study investigated the prognostic heterogeneity of different regulatory T cell (Treg) populations in colorectal cancer (CRC) using multiplex fluorescence immunohistochemistry and digital image analysis. The results showed that Treg infiltration in tumors was strongly correlated with the overall amount of T cells and was associated with a favorable prognosis among CRC patients who underwent complete tumor resection. However, high expression of the activation marker CD25 in Tregs was independently associated with an adverse outcome. Moreover, spatial analysis revealed a significant proximity between Tregs and CD8+ cells, which was associated with a poor survival rate. These findings highlight the importance of multi-marker and spatial analysis in accurately predicting patient outcomes in CRC.
Article
Oncology
Jorgen Smeby, Anita Sveen, Christian H. Bergsland, Ina A. Eilertsen, Stine A. Danielsen, Peter W. Eide, Merete Hektoen, Marianne G. Guren, Arild Nesbakken, Jarle Bruun, Ragnhild A. Lothe