Article
Microbiology
Xia Li, Kai Wang, Gerun Wang, Binbin Cui, Shihao Song, Xiuyun Sun, Yinyue Deng
Summary: This study identified oridonin as a compound capable of interfering with QS signaling and physiology in B. cenocepacia. Oridonin inhibited the production of BDSF and AHL signals and attenuated virulence in B. cenocepacia. It also impaired QS-regulated phenotypes in various Burkholderia species. These findings suggest that oridonin could potentially be developed as a new antibacterial agent.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Lujun Yin, Yingjie Wang, Shiliang Xiang, Kaizhong Xu, Bo Wang, Ai-Qun Jia
Summary: In this study, it was found that tyramine could inhibit the production of virulence factors in Burkholderia cenocepacia by blocking the quorum sensing systems. It was also shown to have low toxicity and potential as a combination therapy with traditional antibiotics.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Mingfang Wang, Xia Li, Shihao Song, Chaoyu Cui, Lian-Hui Zhang, Yinyue Deng
Summary: This review summarizes the history and current progress of research on diffusible signal factor (DSF)-type signals in bacterial quorum sensing (QS). Burkhoideria diffusible signal factor (BDSF), a specific DSF-type signal, has been identified in addition to the originally discovered DSF signal in Xanthomonas campestris pv. campestris. The potential of targeting this QS system as a new therapeutic strategy to control bacterial infections and diseases is also discussed.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xin-Fu Yan, Chunxi Yang, Mingfang Wang, Yonlada Yong, Yinyue Deng, Yong-Gui Gao
Summary: GtrR is a global transcriptional regulator downstream RpfR, recognizing GTP and possessing moderate GTPase activity. Specific residues like K170, D236, R311, and R357 are crucial for its biological function, mutation of any leading to complete abolishment of GtrR activity.
Article
Biotechnology & Applied Microbiology
Kai Wang, Xia Li, Chunxi Yang, Shihao Song, Chaoyu Cui, Xiaofan Zhou, Yinyue Deng
Summary: The study demonstrated a new downstream regulator, Bcal3178, which controls biofilm formation and protease production in Burkholderia cenocepacia through modulation by AHL and BDSF QS systems. Bcal3178 regulates target gene expression by directly binding to promoter DNA regions, and it is directly controlled by the AHL system regulator CepR. These results unveil a novel molecular mechanism by which QS systems regulate target gene expression and biological functions in B. cenocepacia.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Immunology
Melanie Gonzales, Laure Plener, Jean Armengaud, Nicholas Armstrong, Eric Chabriere, David Daude
Summary: This study demonstrates the importance of Quorum sensing in Burkholderia thailandensis using an enzyme-based quorum quenching strategy. The disruption of quorum sensing greatly affects bacterial behavior, including motility, proteolytic activity, and antimicrobial molecule production. Furthermore, the study shows that quorum sensing disruption significantly enhances the bactericidal activity against fungi and yeast. These findings are of prime interest for understanding the virulence of Burkholderia species and developing alternative treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Yamin Shen, Shuji Gao, Qingying Fan, Jing Zuo, Yuxin Wang, Li Yi, Yang Wang
Summary: Quorum sensing (QS) is a communication mechanism that coordinates bacterial pathogens' population response by controlling bacterial communication and influencing the transcriptional expression of multiple genes through signaling molecules. Secretion systems (SS) play a crucial role in bacterial information exchange and virulence, involving the secretion or transfer of virulence factors. QS has been found to directly or indirectly regulate many bacterial SS, impacting bacterial virulence and antibiotic resistance. Understanding the relationship between QS and SS provides a theoretical basis for studying bacterial pathogenicity and developing novel antibacterial drugs.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Olakunle A. Jaiyesimi, Andrew C. McAvoy, David N. Fogg, Neha Garg
Summary: This study compared the metabolomes of three clinical B. cenocepacia strains in synthetic CF sputum medium and a routine laboratory medium, revealing differential production of compound classes and specific metabolites in the presence of the antibiotic trimethoprim. The findings provide insight into environment-dependent production of secondary metabolites by B. cenocepacia strains and suggest future research directions to identify personalized strain-specific regulatory mechanisms. Investigations into whether antibiotics with different mechanisms of action induce similar metabolic alterations could inform development of combination treatments for effective clearance of Burkholderia spp. pathogens.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Multidisciplinary
Jingjie Zhou, Guojun Wu, Jie Zheng, Dyaaaldin Abdalmegeed, Mengxi Wang, Shengwei Sun, Rita-Cindy Aye-Ayire Sedjoah, Yuting Shao, Sen Sun, Zhihong Xin
Summary: In this study, a QS circuit, ComQXPA-P-srfA, was constructed to dynamically regulate the production of plipastatin. By mutating the original promoter P-srfA, two QS promoters MuP(srfA) and MtP(srfA) were obtained, leading to a 3.5-fold increase in plipastatin yield. Integration of ComQXPA into plipastatin mono-producing M-24 resulted in the highest reported yield of 3850 mg/L. Furthermore, four new plipastatins, including a new type with double bonds in the fatty acid side chain, were identified through analysis of fermentation products.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jovana Bakovic, Bess Yi Kun Yu, Daniel Silva, Maria Baczynska, Sew Yeu Peak-Chew, Amy Switzer, Lynn Burchell, Sivaramesh Wigneshweraraj, Muralidharan Vandanashree, Balasubramanian Gopal, Valeriy Filonenko, Mark Skehel, Ivan Gout
Summary: S. aureus AgrA is modified by covalent binding of CoA in response to oxidative or metabolic stress, leading to decreased DNA-binding activity.
Article
Microbiology
Holly Shropshire, Rebekah A. Jones, Maria M. Aguilo-Ferretjans, David J. Scanlan, Yin Chen
Summary: The Burkholderia cepacia complex consists of opportunistic pathogens that cause high mortality rates in cystic fibrosis patients. In response to phosphate limitation, a crucial environmental stress, differentially regulated proteins were identified in the cellular and extracellular proteome of B. cenocepacia. This study provides insight into the metabolic shifts in Burkholderia under phosphorus stress, with implications for further research in understanding the biology of these bacteria.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Bao-Anh Thi Nguyen, Ju-Liang Hsieh, Shou-Chen Lo, Sui-Yuan Wang, Chun-Hsiung Hung, Eugene Huang, Shih-Hsun Hung, Wei-Chih Chin, Chieh-Chen Huang
Summary: In this study, the endophytic bacterium Burkholderia cenocapacia 869T2 was found to degrade the most toxic dioxin congener TCDD by nearly 95% after one-week of an aerobic incubation, with a potential general dioxin degradation pathway proposed. The investigation also revealed the crucial role of a catabolic gene, L-2-haloacid dehalogenase (2-HAD), in dioxin dehalogenation in aerobic conditions. Transcriptional analysis indicated high gene expressions of catabolic genes involved in dioxin metabolism in the presence of TCDD.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Engineering, Environmental
Zhuqiu Sun, Jinying Xi, Chunping Yang, Wenjie Cong
Summary: Quorum sensing (QS) plays a crucial role in controlling microbial aggregation, and the optimization of biological waste treatment systems by QS regulation has gained significant attention. This review summarizes the methods of enhancing and inhibiting QS in biological waste treatment systems and concludes the specific improvements achieved in different treatment systems. Moreover, it reviews the effects of QS regulation methods on biofilm and analyzes the knowledge gaps in current research, providing suggestions for future studies.
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Elisabeth Steiner, Rebecca E. Shilling, Anja M. Richter, Nadine Schmid, Mustafa Fazli, Volkhard Kaever, Urs Jenal, Tim Tolker-Nielsen, Leo Eberl
Summary: The BDSF quorum sensing receptor RpfR is a negative regulator of the bep gene cluster in B. cenocepacia. There is a correlation between intracellular c-di-GMP levels and bep expression, except when the intracellular c-di-GMP level is changed by site directed mutagenesis of the EAL or GGDEF domain of RpfR. RpfR and BerB physically interact and give specificity to the regulation of the bep genes.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Review
Biotechnology & Applied Microbiology
Yuxin Wang, Jianjun Dai, Xuhang Wang, Yang Wang, Fang Tang
Summary: This review paper summarizes the function of quorum sensing systems in the interaction between bacteriophage and their host bacteria, and its potential application in alternative control strategies for antibiotic-resistant and biofilm-forming pathogens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)