Article
Spectroscopy
Elisabeth Siebert, Andrea Schmidt, Stefan Frielingsdorf, Jacqueline Kalms, Uwe Kuhlmann, Oliver Lenz, Patrick Scheerer, Ingo Zebger, Peter Hildebrandt
Summary: This study combined resonance Raman spectroscopy, protein engineering, and X-ray crystallography to investigate the structural properties of iron-sulfur centers and address challenges in spectral analysis of metalloproteins. By using techniques such as protein engineering and selective reduction, the researchers successfully transformed the proximal and medial clusters of the protein variant into standard cubane-type [4Fe-4S] clusters.
JOURNAL OF RAMAN SPECTROSCOPY
(2021)
Article
Multidisciplinary Sciences
Christian Arlt, Kerstin Nutschan, Alexander Haase, Christian Ihling, Dirk Taenzler, Andrea Sinz, R. Gary Sawers
Summary: The research shows that HybG plays a crucial role in the biosynthesis and delivery process of the hydrogenase catalytic subunit, suggesting its shuttle between the assembly complex and the apo-catalytic subunit. These findings provide new insights into how organometallic cofactors are assembled and transferred to their client proteins.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Giorgio Caserta, Sven Hartmann, Casey Van Stappen, Chara Karafoulidi-Retsou, Christian Lorent, Stefan Yelin, Matthias Keck, Janna Schoknecht, Ilya Sergueev, Yoshitaka Yoda, Peter Hildebrandt, Christian Limberg, Serena DeBeer, Ingo Zebger, Stefan Frielingsdorf, Oliver Lenz
Summary: In this study, the maturation process of [NiFe]-hydrogenase was investigated using biochemical analysis and various spectroscopic techniques, providing detailed insights into its complex maturation mechanism.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Patricia Concordio-Reis, Sonia S. Ferreira, Vitor D. Alves, Xavier Moppert, Jean Guezennec, Manuel A. Coimbra, Maria A. M. Reis, Filomena Freitas
Summary: This study investigated the chemical composition and rheological properties of a novel exopolysaccharide (EPS), which showed that it had the ability to increase viscosity with concentration, exhibited non-Newtonian shear thinning behavior at concentrations above 0.1 wt%, and demonstrated weak gel-like structure and thermal stability. It also showed good recoverability and self-healing properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Plant Sciences
Bing Yang, Chenyun Xu, Yuting Cheng, Ting Jia, Xueyun Hu
Summary: Iron-sulfur (Fe-S) clusters are ancient protein cofactors that exist ubiquitously in organisms and play important roles in various life processes. Plastids, semi-autonomous organelles believed to originate from cyanobacterial endosymbionts, have a Fe-S cluster biosynthesis and delivery pathway similar to cyanobacteria. Fe-S clusters are crucial for the normal functioning of downstream Fe-S proteins in plastids. Recent research has made significant progress in understanding this pathway and this review summarizes these findings as well as the remaining scientific challenges.
PLANT CELL REPORTS
(2023)
Article
Microbiology
Gerrit J. Schut, Dominik K. Haja, Xiang Feng, Farris L. Poole, Huilin Li, Michael W. W. Adams
Summary: Microorganisms use electron bifurcating enzymes to carry out unfavorable reactions. The HydABC enzyme is found to be common in the microbial world and has various functionalities. A new class of electron bifurcating enzyme, the Bfu family, is proposed to be associated with specific microbial phyla.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jia-Yi Cao, Ying-Ying Wang, Min-Nan Wu, Zhou-Yan Kong, Jing-Hao Lin, Ting Ling, Si-Min Xu, Shuo-Nan Ma, Lin Zhang, Cheng-Xu Zhou, Xiao-Jun Yan, Ji-Lin Xu
Summary: The research shows that the marine heterotrophic bacterium Alteromonas macleodii can enhance the growth of Isochrysis galbana, inhibit stress responses, enhance photosynthesis and biosynthesis, and protect the algae from oxidative stress. There may be an exchange of fixed organic carbon and other biomolecules between the autotroph and heterotroph in the co-culture of I. galbana and A. macleodii.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Patricia Concordio-Reis, Bruno Serafim, Joao R. Pereira, Xavier Moppert, Jean Guezennec, Maria A. M. Reis, Filomena Freitas
Summary: In this study, a marine bacterium named Alteromonas macleodii Mo169 was cultivated using sugar-rich apple pulp waste as the sole carbon source. The strain efficiently utilized the glucose and fructose in the waste to produce biomass and EPS. The compositional analysis of the EPS revealed the presence of various sugar monomers, possibly derived from a high molecular weight polysaccharide in the apple pulp waste. The EPS also exhibited good thickening capacity, making it suitable for various applications.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Multidisciplinary Sciences
Xiang Feng, Gerrit J. Schut, Dominik K. Haja, Michael W. W. Adams, Huilin Li
Summary: Electron bifurcation allows energetically unfavorable biochemical reactions to occur. This study reports a new bifurcating flavoenzyme structure that couples hydrogen oxidation with NAD reduction through electron transfer. By elucidating its structure and function, we gain a deeper understanding of the electron transfer pathways in hydrogenases.
Article
Biochemistry & Molecular Biology
Jing Du, Zhaoyang Huang, Yanchun Li, Xueying Ren, Chaoting Zhou, Ruolan Liu, Ping Zhang, Guojie Lei, Jianxin Lyu, Jianghui Li, Guoqiang Tan
Summary: Copper is a necessary mineral nutrient but excess copper is cytotoxic. Wilson's disease is an autosomal recessive hereditary disease characterized by pathological copper accumulation in organs. The molecular mechanism of Wilson's disease is not fully understood and further research is needed to develop therapeutic strategies.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Batuhan Balci, Roark D. O'Neill, Eric M. Shepard, Adrien Pagnier, Alexander Marlott, Michael T. Mock, William E. Broderick, Joan B. Broderick
Summary: We investigated the maturation of [FeFe]-hydrogenase by using a synthetic complex [Fe-2(& mu;-SH)(2)(CN)(2)(CO)(4)](2-) and the components of glycine cleavage system together with HydF, without the presence of the maturases HydE and HydG. This semisynthetic and fully-defined maturation process offers new insights into the biosynthesis of H-cluster.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Carolyn E. Lubner, Jacob H. Artz, David W. Mulder, Aisha Oza, Rachel J. Ward, S. Garrett Williams, Anne K. Jones, John W. Peters, Ivan I. Smalyukh, Vivek S. Bharadwaj, Paul W. King
Summary: Redox cofactors play a crucial role in mediating electron transfer in biological enzymes. One example is the [FeFe]-hydrogenase I from Clostridium acetobutylicum, which utilizes a terminal, non-canonical, His-coordinated, [4Fe-4S] cluster to mediate interfacial electron transfer. Substituting His for Cys in this enzyme results in changes in electron transfer properties and reactivity, demonstrating the importance of His coordination in controlling electron exchange.
Article
Chemistry, Inorganic & Nuclear
Debashis Basu, Danielle L. Gray, Toby J. Woods, Thomas B. Rauchfuss, Federica Arrigoni, Giuseppe Zampella
Summary: In the field of bioorganometallic chemistry, progress has been made in studying mimics of the active sites of [NiFe]-hydrogenases, with challenges arising in the conversion and misassignment when using Ni(N2S2) derivatives.
Article
Chemistry, Multidisciplinary
Takeshi Hiromoto, Koji Nishikawa, Seiya Inoue, Hideaki Ogata, Yuta Hori, Katsuhiro Kusaka, Yu Hirano, Kazuo Kurihara, Yasuteru Shigeta, Taro Tamada, Yoshiki Higuchi
Summary: This study reports that [NiFe]-hydrogenase from Desulfovibrio vulgaris Miyazaki F can recover its catalytic activity by reacting with H2 under anaerobic conditions after being inactivated in the presence of O2. Neutron structure analysis revealed that a part of the Ni ion dissociates from the active site Ni-Fe complex and forms a new square-planar Ni complex, accompanied by rearrangement of the coordinated thiolate ligands. The analysis also found that the Cys17(S) thiolate forms an unusual hydrogen bond with the main-chain amide N atom of Gly19(S), providing insights into the redox reaction of the Fe-S cluster.
Article
Biochemistry & Molecular Biology
Jing Zhang, Zechen Bai, Min Ouyang, Xiumei Xu, Haibo Xiong, Qiang Wang, Bernhard Grimm, Jean-David Rochaix, Lixin Zhang
Summary: Fe-S clusters are ancient and important for life processes. The biogenesis of Fe-S clusters involves iron acquisition, sulfur mobilization, and cluster formation. Two Arabidopsis DnaJ proteins, DJA6 and DJA5, play a crucial role in facilitating iron incorporation into Fe-S clusters during chloroplast Fe-S cluster biogenesis. Loss of these proteins leads to defects in chloroplast Fe-S protein accumulation and photosynthesis dysfunction. Evolutionary analysis shows that DJA6 and DJA5 are highly conserved in photosynthetic organisms and have a strong evolutionary relationship with SUFE1, SUFC, and SUFD.
Article
Biochemistry & Molecular Biology
Yao Xu, Philip D. Weyman, Miki Umetani, Jing Xiong, Ximing Qin, Qing Xu, Hideo Iwasaki, Carl Hirschie Johnson
Article
Microbiology
Philip D. Weyman, Brenda Pratte, Teresa Thiel
FEMS MICROBIOLOGY LETTERS
(2010)
Article
Microbiology
Philip D. Weyman, Hamilton O. Smith, Qing Xu
FEMS MICROBIOLOGY LETTERS
(2011)
Article
Biochemical Research Methods
Bogumil J. Karas, Jelena Jablanovic, Lijie Sun, Li Ma, Gregory M. Goldgof, Jason Stam, Adi Ramon, Micah J. Manary, Elizabeth A. Winzeler, J. Craig Venter, Philip D. Weyman, Daniel G. Gibson, John I. Glass, Clyde A. Hutchison, Hamilton O. Smith, Yo Suzuki
Article
Biochemical Research Methods
Bogumil J. Karas, Jelena Jablanovic, Edward Irvine, Lijie Sun, Li Ma, Philip D. Weyman, Daniel G. Gibson, John I. Glass, J. Craig Venter, Clyde A. Hutchison, Hamilton O. Smith, Yo Suzuki
Article
Multidisciplinary Sciences
Philip D. Weyman, Walter A. Vargas, Yingkai Tong, Jianping Yu, Pin-Ching Maness, Hamilton O. Smith, Qing Xu
Article
Biochemical Research Methods
Vladimir N. Noskov, Bogumil J. Karas, Lei Young, Ray-Yuan Chuang, Daniel G. Gibson, Ying-Chi Lin, Jason Stam, Isaac T. Yonemoto, Yo Suzuki, Cynthia Andrews-Pfannkoch, John I. Glass, Hamilton O. Smith, Clyde A. Hutchison, J. Craig Venter, Philip D. Weyman
ACS SYNTHETIC BIOLOGY
(2012)
Article
Biochemical Research Methods
Isaac T. Yonemoto, Christopher W. Matteri, Thao Amy Nguyen, Hamilton O. Smith, Philip D. Weyman
JOURNAL OF BIOLOGICAL ENGINEERING
(2013)
Article
Biochemical Research Methods
Bogumil J. Karas, Bhuvan Molparia, Jelena Jablanovic, Wolfgang J. Hermann, Ying-Chi Lin, Christopher L. Dupont, Christian Tagwerker, Isaac T. Yonemoto, Vladimir N. Noskov, Ray-Yuan Chuang, Andrew E. Allen, John I. Glass, Clyde A. Hutchison, Hamilton O. Smith, J. Craig Venter, Philip D. Weyman
JOURNAL OF BIOLOGICAL ENGINEERING
(2013)
Article
Biochemistry & Molecular Biology
Philip D. Weyman, Zhiqiang Pan, Qin Feng, David G. Gilchrist, Richard M. Bostock
PLANT MOLECULAR BIOLOGY
(2006)