Article
Biology
Daria S. Morozova, Alexey A. Martyanov, Sergei Obydennyi, Julia-Jessica D. Korobkin, Alexey Sokolov, Ekaterina Shamova, Irina Gorudko, Anna L. Khoreva, Anna Shcherbina, Mikhail A. Panteleev, Anastasia N. Sveshnikova
Summary: In this study, an ex vivo experimental model for observation of granulocyte activity in thrombus formation was developed. Results showed that granulocytes from patients with WAS had reduced motility compared to healthy donors. The study suggests that WASP deficiency disrupts granulocyte behavior in thromboinflammatory conditions.
Article
Cell Biology
Madeleine Goeritzer, Stefanie Schlager, Katharina B. Kuentzel, Nemanja Vujic, Melanie Korbelius, Silvia Rainer, Dagmar Kolb, Marion Mussbacher, Manuel Salzmann, Waltraud C. Schrottmaier, Alice Assinger, Axel Schlagenhauf, Corina T. Madreiter-Sokolowski, Sandra Blass, Thomas O. Eichmann, Wolfgang F. Graier, Dagmar Kratky
Summary: Loss of ATGL in platelets is associated with an anti-thrombogenic phenotype without pathological bleeding and platelet activation.
Review
Biochemistry & Molecular Biology
Jinmi Zou, Frauke Swieringa, Bas de Laat, Philip G. de Groot, Mark Roest, Johan W. M. Heemskerk
Summary: This paper reviews the functional reversibility of integrin alpha IIb beta 3 activation and transient aggregation and discusses the signaling pathways involved. The study finds that ADP P2Y(1) and P2Y(12) receptor signaling via phosphoinositide 3-kinases and Akt are the principal pathways linked to reversible integrin activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jeremy A. Nestele, Anne-Katrin Rohlfing, Valerie Dicenta, Alexander Bild, Daniela Eissler, Frederic Emschermann, Marcel Kremser, Konstantin Krutzke, Tilman E. Schaeffer, Oliver Borst, Moran Levi, Netanel Korin, Meinrad Paul Gawaz
Summary: Nanoparticles provide a novel approach for targeted antithrombotic therapy, delivering drugs to specific sites with low side effects. In this study, nanoparticles combined with established proteins successfully inhibited platelet aggregation and thrombus formation, indicating a promising therapeutic strategy for antithrombotic treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Amro Elgheznawy, Patricia Oeftering, Maximilian Englert, Kristina Mott, Friederike Kaiser, Charly Kusch, Uwe Gbureck, Michael R. Boesl, Harald Schulze, Bernhard Nieswandt, Timo Voegtle, Heike M. Hermanns
Summary: Zinc (Zn2+) is an important mediator of immune cell function, thrombosis, and haemostasis. However, the mechanisms that regulate Zn2+ homeostasis in platelets are not well understood. Our study focused on the role of Zn2+ transporters, ZIP1 and ZIP3, in platelet function and Zn2+ homeostasis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dawid Polak, Marcin Talar, Nina Wolska, Dagmara W. Wojkowska, Kamil Karolczak, Karol Kramkowski, Tomasz A. Bonda, Cezary Watala, Tomasz Przygodzki
Summary: The A(2A) agonist HE-NECA in low doses in combination with inhibitors of P2Y(12) improved anti-thrombotic properties and was effective with chronic administration. Additionally, HE-NECA has hypotensive effects but does not significantly affect blood-brain barrier permeability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jingnan Huang, Natalie J. Jooss, Delia Fernandez, Albert Sickmann, Angel Garcia, Kanin Wichapong, Ingrid Dijkgraaf, Johan W. M. Heemskerk
Summary: This study reveals a shear-dependent signaling axis of PTK2, integrin alpha IIb beta 3, and CIB1 in collagen- and GPVI-dependent thrombus formation, which is modulated by GPR56.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Francesco Andreata, Marc Clement, Robert A. Benson, Juliette Hadchouel, Emanuele Procopio, Guillaume Even, Julie Vorbe, Samira Benadda, Veronique Ollivier, Benoit Ho-Tin-Noe, Marie Le Borgne, Pasquale Maffia, Antonino Nicoletti, Giuseppina Caligiuri
Summary: Effective neutrophil migration to sites of inflammation is crucial for host immunity. This study focuses on the role of platelet-endothelial cell adhesion molecule (Pecam1, also known as CD31) in neutrophil migration. The study found that CD31 is required for actin/integrin polarization in migrating neutrophils and acts as a molecular rheostat controlling integrin-mediated adhesion. CD31 co-signaling actively promotes uropod detachment and effective migration of neutrophils to sites of inflammation.
Article
Hematology
Xiang Gui, Xiang Chu, Yuwei Du, Yuhan Wang, Sixuan Zhang, Yangyang Ding, Huan Tong, Mengdi Xu, Yue Li, Wen Ju, Zengtian Sun, Zhenyu Li, Lingyu Zeng, Kailin Xu, Jianlin Qiao
Summary: PDE5A deficiency prolongs bleeding time and delays arterial and venous thrombus formation. It inhibits platelet aggregation and activation, reduces phosphatidylserine exposure, calcium mobilization, and reactive oxygen species production, increases intracellular cGMP level, and modulates platelet function and thrombus formation.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Kristin Jahn, Stefan Handtke, Raghavendra Palankar, Thomas P. Kohler, Jan Wesche, Martina Wolff, Janina Bayer, Christiane Wolz, Andreas Greinacher, Sven Hammerschmidt
Summary: This study investigated the effects of Staphylococcus aureus and Streptococcus pneumoniae toxins on human platelets. The results showed that alpha-hemolysin of S. aureus had a significant impact on platelet activation and thrombus formation, while other toxins had little effect. These findings may have clinical relevance for the treatment of S. aureus-induced endocarditis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biochemistry & Molecular Biology
Dan Shu, Ying Zhu, Meng Lu, Ao-Di He, Jiang-Bin Chen, Ding-Song Ye, Yue Liu, Xiang-Bin Zeng, Rong Ma, Zhang-Yin Ming
Summary: Sanguinarine demonstrates antiplatelet activity by inhibiting thrombus formation and platelet activation through multiple molecular mechanisms. This indicates its potential clinical value in treating cardiovascular diseases involving excessive platelet activation.
Article
Pharmacology & Pharmacy
Jianrong Yang, Jingtao Zhong, Mi Zhou, Yinghong Zhou, Peng Xiu, Feng Liu, Fuhai Wang, Zelun Li, Yuntian Tang, Yuanyuan Chen, Siyang Yao, Tao Huang, Tianqi Liu, Xiaofeng Dong
Summary: T7 peptide inhibits endothelial cell proliferation, migration, and tube formation, and promotes apoptosis under different oxygen conditions via integrin α3β1 and αvβ3 pathways. The combination of T7 peptide and meloxicam enhances the anti-tumor effect against HCC tumors.
Article
Oncology
Zheming Liu, Jing Wang, Fuben Liao, Qibin Song, Yi Yao
Summary: Platelets are small anucleate cells that circulate in the blood and form thrombi. Tumor-educated platelets derived from cancer patients play a role in cancer-associated thrombosis. Studies show that tumor-educated platelets from melanoma-burdened mouse models demonstrate enhanced migration and clot formation, but are inhibited by plasma from tumor mice. RNA sequence profile of tumor-educated platelets reveals upregulation of genes associated with cell migration and cytoskeleton, shedding light on the process of thrombus formation.
FRONTIERS IN ONCOLOGY
(2022)
Article
Hematology
Jan Zlamal, Karina Althaus, Hisham Jaffal, Helene Haberle, Lisann Pelzl, Anurag Singh, Andreas Witzemann, Karoline Weich, Michael Bitzer, Nisar Malek, Siri Gopel, Hans Bosmuller, Meinrad Gawaz, Valbona Mirakaj, Peter Rosenberger, Tamam Bakchoul
Summary: Thromboembolic events are frequently seen in COVID-19 patients, but the mechanisms behind COVID-19-associated hypercoagulopathy are not fully understood. This study reveals that COVID-19 patients with severe infection have platelets expressing high levels of procoagulant markers, which are associated with increased thrombosis risk. The study also identifies platelet-reactive IgG antibodies as the cause of procoagulant platelet formation, and suggests that upregulation of cyclic-adenosine-monophosphate (cAMP) could be a potential therapeutic target for preventing antibody-mediated coagulopathy in COVID-19.
Article
Multidisciplinary Sciences
Estelle Carminita, Lydie Crescence, Nicolas Brouilly, Alexandre Altie, Laurence Panicot-Dubois, Christophe Dubois
Summary: The research indicates that neutrophils rather than NETs are involved in thrombus formation after laser-induced injury. Treatment with DNase-I leads to hydrolysis of ATP and ADP, generating adenosine and inhibiting thrombus formation in vivo.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Hematology
B. Boylan, C. H. Miller
Letter
Hematology
C. H. Miller, A. B. Payne, J. Driggers, D. Ellingsen, B. Boylan, C. J. Bean
Article
Multidisciplinary Sciences
Nathalie Cloutier, Isabelle Allaeys, Genevieve Marcoux, Kellie R. Machlus, Benoit Mailhot, Anne Zufferey, Tania Levesque, Yann Becker, Nicolas Tessandier, Imene Melki, Huiying Zhi, Guy Poirier, Matthew T. Rondina, Joseph E. Italiano, Louis Flamand, Steven E. McKenzie, Francine Cote, Bernhard Nieswandt, Waliul I. Khan, Matthew J. Flick, Peter J. Newman, Steve Lacroix, Paul R. Fortin, Eric Boilard
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Hematology
Claire Kizlik-Masson, Quentin Deveuve, Yuhang Zhou, Caroline Vayne, Gilles Thibault, Steven E. McKenzie, Claire Pouplard, Stephane Loyau, Yves Gruel, Jerome Rollin
Letter
Hematology
Amanda B. Payne, Connie H. Miller, Dorothy Ellingsen, Jennifer Driggers, Brian Boylan, Christopher J. Bean
Article
Health Care Sciences & Services
Sanaa Rizk, David Axelrod, Gaye Riddick-Burden, Elisabeth Congdon-Martin, Steven McKenzie, Carol Haines, Lawrence Ward, John McAna, Albert G. Crawford
AMERICAN JOURNAL OF MEDICAL QUALITY
(2020)
Article
Hematology
Brian Boylan, Glenn P. Niemeyer, Bonnie Werner, Connie H. Miller
Summary: This study aimed to evaluate the anti-FVIII antibody response in patients receiving ITI or BPA, showing a higher frequency of anti-FVIII IgG(4) positivity. Analysis of anti-FVIII antibody levels in patients undergoing ITI revealed that antibodies can persist even after the patient's NBA result falls into the negative range.
Letter
Hematology
Connie H. Miller, Brian Boylan
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Editorial Material
Hematology
Steven E. McKenzie
Summary: The study found that COVID-19 infection leads to aberrant glycosylation of anti-spike IgG, resulting in increased prothrombotic platelet activation.
Article
Hematology
Stephanie A. Renna, James Michael, Xianguo Kong, Lin Ma, Peisong Ma, Marvin T. Nieman, Leonard C. Edelstein, Steven E. McKenzie
Summary: The study found functional differences between human and mouse PAR4, including responsiveness and signaling pathways, highlighting the importance of considering these differences when interpreting mouse PAR4 studies.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Rupsa C. Boelig, Tara J. Cahanap, Lin Ma, Tingting Zhan, Vincenzo Berghella, Joanna S. Y. Chan, Walter K. Kraft, Steven E. Mckenzie
Summary: The PAR4 Thr120 variant of the platelet receptor is associated with an increased risk of placental vascular pathology and preterm birth in homozygous individuals.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Editorial Material
Hematology
Steven E. McKenzie
Article
Hematology
Stephanie A. Renna, Xuefei Zhao, Satya P. Kunapuli, Peisong Ma, Michael Holinstat, Matthew B. Boxer, David J. Maloney, James V. Michael, Steven E. Mckenzie
Summary: This study investigated the cooperativity of PARs (protease-activated receptors) and Fc & gamma;RIIA in HIT using human and mouse model systems. It was found that pharmacological inhibition of 12-LOX can decrease platelet procoagulant phenotype and reduce thrombocytopenia and thrombosis in a mouse model of HIT.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Letter
Hematology
Connie H. Miller, Brian Boylan, Amanda B. Payne, Jennifer Driggers, Christopher J. Bean
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2021)
Article
Hematology
Xi Chen, Shuchi Gupta, Matthew Cooper, Daniel DeHelian, Xuefei Zhao, Meghna U. Naik, Jeremy G. T. Wurtzel, Timothy J. Stalker, Lawrence E. Goldfinger, Jeffrey Benovic, Lawrence F. Brass, Steven E. McKenzie, Ulhas P. Naik, Peisong Ma