Article
Multidisciplinary Sciences
Rhiannon Morris, Yaoyuan Zhang, Julia Ellyard, Carola G. Vinuesa, James M. Murphy, Artem Laktyushin, Nadia J. Kershaw, Jeffrey J. Babon
Summary: The researchers presented structures of the substrate recognition (SH2) domain of LNK in complex with phosphorylated motifs from JAK2 and EPOR, providing insight into its binding specificity and mode of action.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Sophia Koutsogiannaki, Weiming Bu, Wiriya Maisat, Mariel Manzor, Zhikuan Zhang, Umeharu Ohto, Roderic G. Eckenhoff, Koichi Yuki
Summary: Sedatives/anesthetics can modulate immune functions, but their effect on TLR7 function is not well understood. This study found that propofol attenuates TLR7 activation and reduces immune response triggered by TLR7 agonist.
Article
Biochemistry & Molecular Biology
Maddalena Di Sanzo, Flora Cozzolino, Anna Martina Battaglia, Ilenia Aversa, Vittoria Monaco, Alessandro Sacco, Flavia Biamonte, Camillo Palmieri, Francesca Procopio, Gianluca Santamaria, Francesco Ortuso, Piero Pucci, Maria Monti, Maria Concetta Faniello
Summary: The study found that the interaction between FTH1 and PRDX6 in cancer cells can affect cell proliferation and migration, leading to a less invasive phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Raquel Arroyo, Shawn N. Grant, Miriam Colombo, Lucia Salvioni, Fabio Corsi, Marta Truffi, Davide Ottolina, Brett Hurst, Marc Salzberg, Davide Prosperi, Paul S. Kingma
Summary: SARS-CoV-2 infection of host cells is driven by the binding of the virus spike protein to lung cells, and the study aims to measure pulmonary SP-D levels in COVID-19 patients and demonstrate its activity against the virus. Results show that SP-D can bind to the SARS-CoV-2 spike protein, initiate aggregation, and inhibit viral replication in cells.
Article
Biochemistry & Molecular Biology
Anna-Sophia Lieselott Beyer, Daniel Kaemmerer, Joerg Saenger, Katja Evert, Amelie Lupp
Summary: FAM159B, an adaptor protein, plays crucial roles in various cell signalling pathways. Experimental validation showed its expression in neuronal and neuroendocrine tissues and high expression levels in different types of tumors, making the antibody HPA011778 a useful tool for researching and detecting FAM159B expression in tissue samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Anudishi Tyagi, Appalaraju Jaggupilli, Stanley Ly, Bin Yuan, Fouad El-Dana, Venkatesh L. Hegde, Vivek Anand, Bijender Kumar, Mamta Puppala, Zheng Yin, Stephen T. C. Wong, Alexis Mollard, Hariprasad Vankayalapati, Jason M. Foulks, Steven L. Warner, Naval Daver, Gautam Borthakur, V. Lokesh Battula
Summary: The study identified ACVR1 as a factor favoring AML growth and a potential therapeutic target. ACVR1 was found to be overexpressed in FLT3-mutated AML and inhibition of ACVR1 expression increased the sensitivity of AML cells to FLT3 inhibitors. A novel ACVR1 inhibitor, TP-0184, was developed and shown to selectively arrest the growth of FLT3-mutated AML cells.
Article
Multidisciplinary Sciences
Wen-Hung Tang, Chiu-Feng Wang, You-Di Liao
Summary: Some AMPs and drugs are bound and inhibited by serum proteins in complex with alpha 1-antitrypsin in bovine serum only at fetal stage, with TP4's hydrophobic residues responsible for its binding to the complex. This active albumin/alpha 1-antitrypsin complex's existence and depletion are important for biomolecule assay and production due to bovine serum's prevalence in cell culture media.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Muhammad Ali, Eirini Giannakopoulou, Yingqian Li, Madeleine Lehander, Stina Virding Culleton, Weiwen Yang, Cathrine Knetter, Mete Can Odabasi, Ravi Chand Bollineni, Xinbo Yang, Zsofia Foldvari, Maxi-Lu Boschen, Eli Taraldsrud, Erlend Stronen, Mireille Toebes, Amy Hillen, Stefania Mazzi, Arnoud H. de Ru, George M. C. Janssen, Arne Kolstad, Geir Erland Tjonnfjord, Benedicte A. Lie, Marieke Griffioen, Soren Lehmann, Liv Toril Osnes, Jochen Buechner, K. Christopher Garcia, Ton N. Schumacher, Peter A. van Veelen, Matthias Leisegang, Sten Eirik W. Jacobsen, Petter Woll, Johanna Olweus
Summary: T cells modified with TCRs targeting TdT can specifically eliminate acute lymphoblastic leukemia cells while sparing normal lymphocytes. TdT is highly expressed in cancer cells but transiently expressed in normal cells, thus limiting the toxicity of T cell targeting TdT.
NATURE BIOTECHNOLOGY
(2022)
Article
Biology
Paul S. S. Kwon, Shirley Xu, Hanseul Oh, Seok-Joon Kwon, Andre L. Rodrigues, Maisha Feroz, Keith Fraser, Peng He, Fuming Zhang, Jung Joo Hong, Robert J. J. Linhardt, Jonathan S. S. Dordick
Summary: A polysulfated synthetic drug called suramin binds to the ACE2 receptor and heparan sulfate binding sites on SARS-CoV-2 receptor binding domains (RBDs), with preferential binding for the Omicron RBD and inhibition of infection by the Omicron variant in vitro. These findings suggest that suramin and other polysulfated molecules could be potential therapeutic and prophylactic options against COVID-19.
COMMUNICATIONS BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Wenjuan Liu, Zhenqiang Li, Simeng Chu, Xiaoyao Ma, Xiaoying Wang, Min Jiang, Gang Bai
Summary: This study found that atractylenolide-I (AT-I) can selectively inhibit cytochrome P450 11B2 (CYP11B2), thereby reducing the production of aldosterone and showing significant therapeutic effects for treating hyperaldosteronism.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Biochemistry & Molecular Biology
Elizabeth J. Osterlund, Nehad Hirmiz, Dang Nguyen, James M. Pemberton, Qiyin Fang, David W. Andrews
Summary: The ER-resident protein BIK interacts directly with antiapoptotic proteins localized to mitochondria, promoting mitochondrial outer membrane permeabilization and initiating apoptosis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Cell & Tissue Engineering
Anna Bigas, Luis Galan Palma, Gayathri M. Kartha, Alessandra Giorgetti
Summary: Researchers have been studying embryonic stem cells for several decades, aiming to use them in regenerative medicine. The hematopoietic field has been a pioneer in utilizing stem cells for blood cell development and clinical applications. However, generating authentic hematopoietic stem cells has proven to be challenging. While progress has been made in understanding the origin of these cells during embryonic development, reproducing this process in the lab is still not possible. Nevertheless, knowledge gained from studying embryonic development is being applied to pluripotent stem cells derived from mice and humans. Additionally, studies have shown that hematopoietic cells derived from embryonic stem cells can recapitulate some leukemic transformation processes, making them valuable for modeling prenatal leukemia development and other leukemia-predisposing syndromes.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Shijie Huang, Arpan Bhattacharya, Mikel D. Ghelfi, Hong Li, Clark Fritsch, David M. Chenoweth, Yale E. Goldman, Barry S. Cooperman
Summary: This study identifies the binding sites of the translation readthrough inducing drug (TRID) ataluren on the ribosome, and reveals how it inhibits termination at nonsense codons.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Youngchang Kim, Jacek Wower, Natalia Maltseva, Changsoo Chang, Robert Jedrzejczak, Mateusz Wilamowski, Soowon Kang, Vlad Nicolaescu, Glenn Randall, Karolina Michalska, Andrzej Joachimiak
Summary: SARS-CoV-2 Nsp15 is a uridine-specific endoribonuclease that interferes with the innate immune response and is a potential target for therapeutic intervention. Studies have shown that Tipiracil inhibits the activity of Nsp15 by interacting with the uridine binding pocket. This discovery provides new insights for the development of drugs based on uracil scaffold.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Oleg M. Panasenko, Viktor A. Ivanov, Elena V. Mikhalchik, Irina V. Gorudko, Daria V. Grigorieva, Liliya Yu. Basyreva, Ekaterina V. Shmeleva, Sergey A. Gusev, Valeria A. Kostevich, Nikolay P. Gorbunov, Alexey V. Sokolov
Summary: Hyperglycemia in diabetes mellitus alters the functionality of neutrophils, reducing their bactericidal activity mediated by MPO, which is affected by the modification of proteins by glucose and its derivative methylglyoxal. This phenomenon may contribute to impaired wound healing and increased susceptibility to infection in patients with hyperglycemia.
Article
Cell Biology
Kristina B. Emdal, Nicolas Palacio-Escat, Caroline Wigerup, Akihiro Eguchi, Helen Nilsson, Dorte B. Bekker-Jensen, Lars Ronnstrand, Julhash U. Kazi, Alexandre Puissant, Raphael Itzykson, Julio Saez-Rodriguez, Kristina Masson, Peter Blume-Jensen, Jesper V. Olsen
Summary: In this study, the signaling responses of Selinexor in AML patients and cell lines were investigated using phosphoproteomics. The study identified key targetable signaling hubs for rational drug combinations by scoring the phosphorylation sites of P53. Furthermore, combining Selinexor with AKT inhibitor MK-2206 overcame dysregulated AKT-FOXO3 signaling in resistant cells, resulting in synergistic anti-proliferative effects.
Article
Chemistry, Multidisciplinary
Pei Huang, Lingsheng Jiang, Hui Pan, Lingwen Ding, Bo Zhou, Mengyao Zhao, Jianhua Zou, Benhao Li, Meiwei Qi, Hongzhang Deng, Yongfeng Zhou, Xiaoyuan Chen
Summary: Researchers synthesized a new polymer for mRNA delivery, which showed no inflammatory side effects in vivo and successfully delivered mRNA cancer vaccines, resulting in robust anti-tumor cellular immune response.
ADVANCED MATERIALS
(2023)
Article
Oncology
Xue-Bin Ran, Ling-Wen Ding, Qiao-Yang Sun, Henry Yang, Jonathan W. Said, Lao Zhentang, Vikas Madan, Pushkar Dakle, Jin-Fen Xiao, Xinyi Loh, Ying Li, Liang Xu, Xiao-Qiang Xiang, Ling-Zhi Wang, Boon Cher Goh, De-Chen Lin, Wee Joo Chng, Soo-Yong Tan, Sudhakar Jha, H. Phillip Koeffler
Summary: Despite the limited response rate of immune checkpoint blockade therapy, targeting RNA decay machinery by silencing XRN1 can sensitize tumor cells to immunotherapy by activating IFN signaling and the viral defense pathway. XRN1 depletion triggers aberrant RNA-mediated IFN signaling and has the potential to be effective in multiple types of cancers. These findings provide a molecular rationale for developing XRN1 inhibitors and combining them with cancer immunotherapy.
Article
Medicine, Research & Experimental
Win Lwin Thuya, Li Ren Kong, Nicholas L. Syn, Ling -Wen Ding, Esther Sok Hwee Cheow, Regina Tong Xin Wong, Tingting Wang, Robby Miguel Wen-Jing Goh, Hongyan Song, Migara K. Jayasinghe, Minh T. N. Le, Jian Cheng Hu, Wei-Peng Yong, Soo-Chin Lee, Andrea Li-Ann Wong, Gautam Sethi, Huynh The Hung, Paul Chi-Lui Ho, Jean-Paul Thiery, Siu Kwan Sze, Tiannan Guo, Ross A. Soo, Henry Yang, Yaw Chyn Lim, Lingzhi Wang, Boon-Cher Goh
Summary: This study reveals that the cargo protein FAM3C carried by tumor-released extracellular vesicles (EVs) can enhance the growth and metastatic potential of lung cancer cells. FAM3C interacts with RalA protein to activate the Src/Stat3 signaling pathway, promoting oncogenicity. This finding provides a new target for lung cancer therapy.
Article
Biochemistry & Molecular Biology
Ahmad Nasimian, Mehreen Ahmed, Ingrid Hedenfalk, Julhash U. Kazi
Summary: In this study, a gene signature was defined through the analysis of cisplatin-perturbed gene expression and pathway enrichment, and a cisplatin sensitivity prediction model was developed using the TabNet algorithm. The TabNet model outperformed other machine learning models with an accuracy of over 80%. Furthermore, BCL2L1 was identified as an important gene contributing to cisplatin resistance, and its pharmacological inhibition was found to enhance cisplatin efficacy. This study developed a tool to predict cisplatin sensitivity and identified BCL2L1 as a significant gene in this context.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Ahmad Nasimian, Lina Al Ashiri, Mehreen Ahmed, Hongzhi Duan, Xiaoyue Zhang, Lars Roennstrand, Julhash U. Kazi
Summary: Despite progress in cancer treatment, therapy resistance remains a major obstacle for long-term survival. By using highly variable genes and pharmacogenomic data, a drug sensitivity prediction model for the tyrosine kinase inhibitor sorafenib was developed with over 80% accuracy. AXL was identified as an important feature for drug resistance and PKC signaling was found to be enriched in drug-resistant patient samples. Inhibition of tyrosine kinase activity enhanced AXL expression and phosphorylation of the PKC-substrate CREB protein, showing synergy with AXL and PKC inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kinjal Shah, Lina Al Ashiri, Ahmad Nasimian, Mehreen Ahmed, Julhash U. Kazi
Summary: Therapy resistance in cancer treatment, specifically T-cell acute lymphoblastic leukemia (T-ALL), is linked to the deregulation of the anti-apoptotic protein BCL2 and is mediated through distinct gene signatures and cytokine signaling pathways. Different T-ALL cell lines display varying response to BCL2 inhibition, and prolonged exposure to venetoclax leads to the development of resistance. The expression of BCL2 family genes and global gene expression profiles, including cancer stem cell-related genes, differ between resistant cells and sensitive cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Dorit Blickstein, Marina Izak, Talia Filipovich-Rimon, Osnat Garach-Jehoshua, Naomi Rahimi-Levene, Eilat Shinar, Ramzia Abu Hamad, Adina Bar-Chaim, Maya Koren-Michowitz
Summary: This study aimed to evaluate the prevalence of antiphospholipid antibodies (APLA) in convalescent plasma (CP) obtained from COVID-19 patients and assess the potential risk of thrombosis in transfused patients. The results showed that out of 122 CP samples, 7 samples (6%) tested positive for APLA. The low prevalence of APLA in CP donors reassured the safety of CP administration to patients with severe COVID-19.
Article
Biochemistry & Molecular Biology
Sigal Avraham, Leonie Schuetz, Larissa Kaever, Andreas Dankers, Sapir Margalit, Yael Michaeli, Shahar Zirkin, Dmitry Torchinsky, Noa Gilat, Omer Bahr, Gil Nifker, Maya Koren-Michowitz, Elmar Weinhold, Yuval Ebenstein
Summary: The article presents a new high-throughput platform for multi-color epigenetic analysis, which allows simultaneous detection of methylation and demethylation signals. By utilizing an engineered methyltransferase enzyme and enzymatic glycosylation, the study achieves simultaneous measurement of 5-hydroxymethylcytosine and 5-methylcytosine. The findings demonstrate the potential of using a simple blood test for epigenetic evaluation in clinical samples, benefiting research and patient management.
Article
Biochemistry & Molecular Biology
Anbu Liu, Shaoting Zhang, Ming Wang, Liangying Zhang, Shidong Xu, Ahmad Nasimian, Shujing Li, Sien Zhao, Xu Cao, Jinhai Tian, Yuanyuan Yu, Zhaoyang Fan, Kun Xiao, Hui Zhao, Julhash U. Kazi, Lijun Ma, Jianmin Sun
Summary: This study demonstrates that DDR1/2 contribute to KIT activation and enhance resistance to imatinib in GISTs, providing a rationale for further exploration of DDR1/2 targeting in GIST treatment.
MOLECULAR CARCINOGENESIS
(2023)
Article
Oncology
Gideon Ze Lin Tan, Sai Mun Leong, Yu Jin, Chik Hong Kuick, Jeremy Joon Keat Chee, San Zeng Low, Ling-Wen Ding, He Cheng, Diana Lim, Susan Swee-Shan Hue
Summary: In this study, we analyzed the expression of microRNAs in endometrial carcinomas and found differential expression profiles among different subtypes and molecular subtypes. These findings may have implications for the development of diagnostic and prognostic tools for endometrial carcinoma.
Article
Medicine, General & Internal
Srdan Verstovsek, Aaron T. Gerds, Alessandro M. Vannucchi, Haifa Kathrin Al-Ali, David Lavie, Andrew T. Kuykendall, Sebastian Grosicki, Alessandra Iurlo, Yeow Tee Goh, Mihaela C. Lazaroiu, Miklos Egyed, Maria Laura Fox, Donal McLornan, Andrew Perkins, Sung -Soo Yoon, Vikas Gupta, Jean -Jacques Kiladjian, Nikki Granacher, Sung-Eun Lee, Luminita Ocroteala, Francesco Passamonti, Claire N. Harrison, Barbara J. Klencke, Sunhee Ro, Rafe Donahue, Jun Kawashima, Ruben Mesa
Summary: This study aimed to compare the clinical effects of momelotinib and danazol in patients with intermediate or high-risk myelofibrosis. The results showed that momelotinib can significantly improve myelofibrosis-associated symptoms, anemia measures, and spleen response compared to danazol, with favorable safety.
Article
Oncology
Julhash U. Kazi, Lina Al Ashiri, Rituraj Purohit, Lars Ronnstrand
Summary: This study investigated the impact of FLT3 mutations on its function and interaction with therapeutic drugs in acute myeloid leukemia (AML). The findings provide insights for tailored treatments and highlight the significance of customized medical approaches in AML therapy.
Review
Rheumatology
Ofer Levy, Arie Apel, Hossam Alhdor, Avraham Mizrachi, Nancy Agmon-Levin, Maya Koren-Michowitz, Mirit Amit-Vazina
Summary: The translation mentions a case of a patient with adult-onset Still's disease who did not respond to conventional medications but miraculously responded to salvage therapy with ruxolitinib. The article reviews recent data related to the therapeutic value of ruxolitinib for macrophage activation syndrome triggered by adult-onset Still's disease.
EUROPEAN JOURNAL OF RHEUMATOLOGY
(2022)