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Multidisciplinary Sciences
Jingyang Guan, Guan Wang, Jinlin Wang, Zhengyuan Zhang, Yao Fu, Lin Cheng, Gaofan Meng, Yulin Lyu, Jialiang Zhu, Yanqin Li, Yanglu Wang, Shijia Liuyang, Bei Liu, Zirun Yang, Huanjing He, Xinxing Zhong, Qijing Chen, Xu Zhang, Shicheng Sun, Weifeng Lai, Yan Shi, Lulu Liu, Lipeng Wang, Cheng Li, Shichun Lu, Hongkui Deng
Summary: This study demonstrates the chemical reprogramming of human somatic cells to human chemically induced pluripotent stem cells, which exhibit key features of embryonic stem cells. The induction of an intermediate plastic state and inhibition of the JNK pathway are crucial for successful chemical reprogramming.
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Gastroenterology & Hepatology
Yunfei Bai, Zhenghao Yang, Xiaochan Xu, Wanqiu Ding, Juntian Qi, Feng Liu, Xiaoxiao Wang, Bin Zhou, Wenpeng Zhang, Xiaomei Zhuang, Guanglu Li, Yang Zhao
Summary: Cell fate can be directly reprogrammed into functional cell types using small molecule stimuli. This study demonstrates the successful direct chemical reprogramming of mouse fibroblasts into functional hepatocyte-like cells. The chemically induced hepatocytes exhibit comparable activity and function to primary hepatocytes and have high-quality physiological properties. The findings suggest the potential of chemical reprogramming in organ/tissue repair and regeneration therapies.
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Cell Biology
Longfei Sun, Dandan Zhang, Liangshan Qin, Quanhui Liu, Guodong Wang, Deshun Shi, Ben Huang
Summary: In this study, bovine ear fibroblasts were chemically induced to differentiate into adipocyte-like cells using a small-molecule cocktail called RVB. These induced cells (CiADCs) exhibited similar morphology and gene expression patterns to natural adipocytes. Furthermore, RVB increased the expression of PPAR gamma and C/EBP alpha proteins. Signaling pathways including PPAR signaling pathway, PI3K-Akt signaling pathway, p53 signaling pathway, MAPK signaling pathway, and ECM-receptor interaction played a vital role in adipogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Cristiana Mollinari, Daniela Merlo
Summary: Translating successful preclinical research in neurodegenerative diseases into clinical practice has been challenging. Human induced pluripotent stem cells are a powerful tool for studying the molecular mechanisms of disease onset and pathogenesis, but require time to differentiate and may lose age-related information.
NEUROCHEMICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Mougina K. Eltahir, Masayuki Nakamori, Satoshi Hattori, Takashi Kimura, Hideki Mochizuki, Seiichi Nagano
Summary: This study successfully established a neuronal model for DM1 and found faster degeneration, aberrant splicing, and nuclear RNA foci in DM1 hiNeurons. They also tested the effectiveness of two drugs and found that they reduced the number of RNA foci and corrected splicing abnormalities. This research provides a valuable tool and direction for further understanding and treating DM1.
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Multidisciplinary Sciences
Adriaan Merlevede, Emilie M. Legault, Viktor Drugge, Roger A. Barker, Janelle Drouin-Ouellet, Victor Olariu
Summary: Direct reprogramming of adult skin fibroblasts into neurons is controlled by interacting gene regulators, and the dynamics of these interactions are crucial for cellular decision making. A quantitative model of the governing gene regulatory system, supported by mRNA expression measurements, was proposed to capture the accurate gene interaction dynamics and predict the outcome of various experiments. Experimental validation demonstrated the importance of nPTB feedback for successful neural conversion, and a novel analytical technique was introduced to dissect system behavior and reveal the influence of individual factors on gene expression. Overall, computational analysis proves to be a powerful tool for understanding the mechanisms of direct (neuronal) reprogramming and improving cell conversion strategies in the future.
SCIENTIFIC REPORTS
(2021)
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Cell & Tissue Engineering
Jinhua Qin, Jian Zhang, Jianan Jiang, Bowen Zhang, Jisheng Li, Xiaosong Lin, Sihan Wang, Meiqi Zhu, Zeng Fan, Yang Lv, Lijuan He, Lin Chen, Wen Yue, Yanhua Li, Xuetao Pei
Summary: A combination of four small molecules has been found to successfully convert human cord blood erythroblasts into induced megakaryocytes (iMKs) that can produce functional platelets, providing a simple and efficient approach for clinical applications.
Review
Cell Biology
Lakshmy Vasan, Eunjee Park, Luke Ajay David, Taylor Fleming, Carol Schuurmans
Summary: Direct neuronal reprogramming is an innovative technology that converts somatic cells into induced neurons without passing through a pluripotent state. The capacity to create new neurons in the brain has opened up possibilities for treating incurable neurodegenerative diseases and brain injuries. By identifying intrinsic and extrinsic cues, developmental biologists have paved the way for this field, leading to the development of new methodologies for neuronal reprogramming.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Bob A. Hersbach, Tatiana Simon, Giacomo Masserdotti
Summary: Direct neuronal reprogramming is a powerful technique that can generate functional neurons from different starter cell populations without the need for multipotent intermediates. This has great potential in disease modeling and as an alternative for cell-based replacement therapies. The conversion of differentiated non-neural cells, such as astrocytes, into functional neurons in vitro has provided important insights into the molecular mechanisms and challenges of efficient reprogramming. However, comparing results from different labs is difficult due to variations in isolation, culture, and reprogramming methods. This article provides a detailed protocol for reliably isolating and culturing astrocytes with high purity, as well as protocols for reprogramming them into neurons.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Developmental Biology
Koji Tanabe, Hiroko Nobuta, Nan Yang, Cheen Euong Ang, Philip Huie Jr, Sacha Jordan, Michael C. Oldham, David H. Rowitch, Marius Wernig
Summary: Human oligodendrocytes have been successfully generated by directly converting human dermal fibroblasts into induced oligodendrocyte precursor cells (iOPCs). This method is rapid, efficient, and holds great potential for disease modeling and cell transplantation-based therapies.
Article
Cell & Tissue Engineering
Shijia Liuyang, Guan Wang, Yanglu Wang, Huanjing He, Yulin Lyu, Lin Cheng, Zhihan Yang, Jingyang Guan, Yao Fu, Jialiang Zhu, Xinxing Zhong, Shicheng Sun, Cheng Li, Jinlin Wang, Hongkui Deng
Summary: We have developed a robust and chemically defined reprogramming protocol that significantly shortens the induction time for generating human pluripotent stem cells from somatic cells. This protocol promotes cell proliferation and metabolic activities, leading to a more direct reprogramming process.
Review
Neurosciences
Riccardo Bocchi, Giacomo Masserdotti, Magdalena Goetz
Summary: A successful experiment conducted 20 years ago opened up the field of neuronal reprogramming, which has since made significant progress. These studies are not only important for clinical applications, but also provide fundamental insights into the mechanisms of cell conversion into neurons and the maintenance of neuronal identity.
Article
Cell Biology
Katie Schaukowitch, Justyna A. Janas, Marius Wernig
Summary: Direct neuronal reprogramming is a method to convert somatic cells into neuronal cells, which can be used for modeling neuronal diseases and cell replacement without transplantation. Current research focuses on understanding the mechanisms that protect cell identities and overcoming barriers to fate changes. Additionally, advancements have been made in generating specific types of neuronal cells and applying direct neuronal reprogramming to age-related disorders and regenerative medicine.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2023)
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Biochemistry & Molecular Biology
Toshihiro Kurahashi, Chiyoko Nishime, Eiko Nishinaka, Yuji Komaki, Fumiko Seki, Koji Urano, Yoshinori Harada, Toshikazu Yoshikawa, Ping Dai
Summary: Regenerative medicine using cell therapy has the potential to revolutionize the treatment of diseases like spinal cord injury (SCI) that currently have no radical cure. In this study, immature chemical compound-induced neuronal-like (CiN) cells were transplanted into a rat model of SCI, resulting in partial improvement in locomotor recovery and nerve recovery or preservation. Transcriptome analysis revealed that these immature CiN cells highly express hepatocyte growth factor (HGF), which has shown promise as a therapeutic agent against SCI. These findings suggest that immature CiN cells may offer an alternative strategy for regenerative therapy of SCI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Ryohei Eguchi, Momoko Hamano, Michio Iwata, Toru Nakamura, Shinya Oki, Yoshihiro Yamanishi
Summary: In this study, a novel computational method, TRANSDIRE, was developed to predict the transcription factors (TFs) that induce direct reprogramming in various human cell types using multiple omics data. The method successfully predicted TFs for most cell conversions with high accuracy, suggesting its potential practical applications in regenerative medicine.
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Oncology
Hisataka Matsuo, Yoshinori Harada, Takeo Minamikawa, Yoshiyuki Kato, Yasutoshi Murayama, Eigo Otsuji, Tetsuro Takamatsu, Hideo Tanaka
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2017)
Review
Cell Biology
Yasuaki Kumamoto, Yoshinori Harada, Tetsuro Takamatsu, Hideo Tanaka
ACTA HISTOCHEMICA ET CYTOCHEMICA
(2018)
Review
Biochemistry & Molecular Biology
Yukimasa Takeda, Yoshinori Harada, Toshikazu Yoshikawa, Ping Dai
BIOSCIENCE REPORTS
(2018)
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Multidisciplinary Sciences
Suguru Ohira, Hideo Tanaka, Yoshinori Harada, Takeo Minamikawa, Yasuaki Kumamoto, Satoaki Matoba, Hitoshi Yaku, Tetsuro Takamatsu
SCIENTIFIC REPORTS
(2017)
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Multidisciplinary Sciences
Yukimasa Takeda, Yoshinori Harada, Toshikazu Yoshikawa, Ping Dai
SCIENTIFIC REPORTS
(2017)
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Multidisciplinary Sciences
Tsunehisa Yamamoto, Takeo Minamikawa, Yoshinori Harada, Yoshihisa Yamaoka, Hideo Tanaka, Hitoshi Yaku, Tetsuro Takamatsu
SCIENTIFIC REPORTS
(2018)
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Chemistry, Physical
Yasuaki Kumamoto, Kentaro Mochizuki, Kosuke Hashimoto, Yoshinori Harada, Hideo Tanaka, Katsumasa Fujita
JOURNAL OF PHYSICAL CHEMISTRY B
(2019)
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Computer Science, Information Systems
Go Itami, Osamu Sakai, Yoshinori Harada
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Chemistry, Physical
J. Nicholas Taylor, Kentaro Mochizuki, Kosuke Hashimoto, Yasuaki Kumamoto, Yoshinori Harada, Katsumasa Fujita, Tamiki Komatsuzaki
JOURNAL OF PHYSICAL CHEMISTRY B
(2019)
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Biochemistry & Molecular Biology
Khalifa Mohammad Helal, James Nicholas Taylor, Harsono Cahyadi, Akira Okajima, Koji Tabata, Yoshito Itoh, Hideo Tanaka, Katsumasa Fujita, Yoshinori Harada, Tamiki Komatsuzaki
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Oncology
Midori Morita, Hideo Tanaka, Yasuaki Kumamoto, Akihiro Nakamura, Yoshinori Harada, Takehiro Ogata, Koichi Sakaguchi, Tetsuya Taguchi, Tetsuro Takamatsu
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Multidisciplinary Sciences
Tatsuya Matsumoto, Hirohiko Niioka, Yasuaki Kumamoto, Junya Sato, Osamu Inamori, Ryuta Nakao, Yoshinori Harada, Eiichi Konishi, Eigo Otsuji, Hideo Tanaka, Jun Miyake, Tetsuro Takamatsu
SCIENTIFIC REPORTS
(2019)
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Oncology
Tatsuya Matsumoto, Yasutoshi Murayama, Hisataka Matsuo, Kengo Okochi, Naotaka Koshiishi, Yoshinori Harada, Hideo Tanaka, Tetsuro Takamatsu, Eigo Otsuji
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Engineering, Biomedical
Yasuaki Kumamoto, Kentaro Mochizuki, Kosuke Hashimoto, Yoshinori Harada, Hideo Tanaka, Katsumasa Fujita
BIOMEDICAL IMAGING AND SENSING CONFERENCE 2020
(2020)
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Microscopy
Yoshihisa Yamaoka, Yuka Kimura, Yoshinori Harada, Tetsuro Takamatsu, Eiji Takahashi
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2018
(2018)