Article
Biology
Xin Wang, Anjun Jiao, Lina Sun, Wenhua Li, Biao Yang, Yanhong Su, Renyi Ding, Cangang Zhang, Haiyan Liu, Xiaofeng Yang, Chenming Sun, Baojun Zhang
Summary: This study identifies Zfp335 as a crucial transcription factor in the regulatory network controlling T-cell development in mice. It is involved in maintaining iTCR beta expression during the DN to DP transition and activating cell survival signaling, thereby regulating thymocyte development and survival.
Article
Immunology
Thomas Jouve, Johan Noble, Hamza Naciri-Bennani, Celine Dard, Dominique Masson, Gaelle Fiard, Paolo Malvezzi, Lionel Rostaing
Summary: This study investigated the link between early blood transfusion and de novo donor-specific antibodies (dnDSA) development in a cohort of mainly anti-thymocyte globulin (ATG)-induced kidney transplant patients. The results showed that early blood transfusion did not induce dnDSAs in this cohort, but low hemoglobin level was associated with dnDSAs graft survival. This suggests a protective effect of ATG induction therapy on preventing dnDSA development at an initial stage post-transplantation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Joseph O. Humtsoe, Hyun-Su Kim, Leilani Jones, James Cevallos, Philippe Boileau, Fengshen Kuo, Luc G. T. Morris, Patrick Ha
Summary: This study aims to establish an in vitro and in vivo model of a MYB-NFIB fusion protein expressing system in adenoid cystic carcinoma (ACC) cells. By analyzing RNA-seq data from tumors of MYB-NFIB translocation positive ACC patients and MYB-NFIB fusion transcript in ACC patient-derived xenografts (ACCX), the MYB breakpoints and their frequency of occurrence were identified. Based on the identified MYB breakpoints, variants of MYB-NFIB fusion expression system were developed in MYB-NFIB deficient ACC cell lines. The analysis confirmed the expression of MYB-NFIB fusion protein in ACC cells and ACCXs. Furthermore, the recombinant MYB-NFIB fusion protein showed sustained stability and impacted the transcriptional activities of interferon-associated genes compared to wild type MYB. In vivo tumor formation analysis indicated that MYB-NFIB fusion cells have the ability to grow as implanted tumors, although there were no fusion-mediated growth advantages. This expression system may not only be useful in exploring the functional aspects of MYB-NFIB fusion, but also in evaluating effective drug response in vitro and in vivo settings.
Article
Genetics & Heredity
Shanshan Wu, Jianrui Zhang, Yichun Guan, Bingnan Ren, Yuchao Zhang, Xinmi Liu, Kexin Wang, Mingmei Zhang, Zhen Li
Summary: In patients with chromosomal translocation, fertilization rate may be affected by carrier sex and translocation type, with chromosomes 1-5 translocation potentially adversely affecting available blastocyst formation rate. Break sites play no role in fertilization and blastocyst development.
BMC MEDICAL GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Archita Mishra, Ghee Chuan Lai, Leong Jing Yao, Thet Tun Aung, Noam Shental, Aviva Rotter-Maskowitz, Edwin Shepherdson, Gurmit Singh Naranjan Singh, Rhea Pai, Adhika Shanti, Regina Men Men Wong, Andrea Lee, Costerwell Khyriem, Charles Antoine Dutertre, Svetoslav Chakarov, K. G. Srinivasan, Nurhidaya Binte Shadan, Xiao-Meng Zhang, Shabnam Khalilnezhad, Fabien Cottier, Alrina Shin Min Tan, Gillian Low, Phyllis Chen, Yiping Fan, Pei Xiang Hor, Avery Khoo May Lee, Mahesh Choolani, David Vermijlen, Ankur Sharma, Garold Fuks, Ravid Straussman, Norman Pavelka, Benoit Malleret, Naomi McGovern, Salvatore Albani, Jerry Kok Yen Chan, Florent Ginhoux
Summary: During the 2nd trimester of gestation, microbial signals were detected in fetal gut, skin, placenta, and lungs, contributing to fetal immune-priming. Live bacterial strains, including Staphylococcus and Lactobacillus, were identified in fetal tissues, activating memory T cells in the fetal mesenteric lymph node.
Review
Allergy
Erin C. Davis, Vanessa P. Castagna, David A. Sela, Margaret A. Hillard, Samantha Lindberg, Nicholas J. Mantis, Antti E. Seppo, Kirsi M. Jarvinen
Summary: Establishing the gut microbiome in early life is important for long-term health, and breast-feeding plays a significant role in shaping the composition and function of the microbiome. This review aims to summarize the evidence on the effects of breast-feeding on the gut microbiome and discuss the mechanisms behind these effects.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Oncology
Sonja J. Gill, Paul W. G. Wijnhoven, Jacqueline H. L. Fok, Rebecca L. Lloyd, Jonathan Cairns, Joshua Armenia, Jenni Nikkila, Alan Lau, Christopher J. Bakkenist, Susan M. Galbraith, Conchita Vens, Mark J. O'Connor
Summary: Radiopotentiation profiling of DNA damage response inhibitors (DDRi) revealed lower effective concentrations in radiotherapy combinations, with preferential sensitization of p53-deficient cells. Olaparib, ceralasertib, and adavosertib showed moderate increases in radiotherapy dose enhancement with increasing inhibitor concentration, while small increases in AZD0156 and KU-60648 resulted in steep increases in dose enhancement. Multiple concentration testing demonstrated a relationship between drug concentration and radiotherapy effect, providing valuable insights for dose-escalation strategies in clinical trials.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Fisheries
Pacharaporn Angthong, Sage Chaiyapechara, Wanilada Rungrassamee
Summary: The gut microbiome plays a crucial role in the nutrition, development, and disease resistance of animals. The interactions between the microbiome and the immune system are complex and dynamic, with each influencing the other's composition and activity. In shrimp and other aquatic animals, these interactions occur during the early developmental stages and significantly impact the immune responses and overall health of the shrimp. Understanding this interaction has the potential to improve shrimp survival and enhance the microbiome through feed supplements or other strategies.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Erica T. Grant, Marie Boudaud, Arnaud Mueller, Andrew J. Macpherson, Mahesh S. Desai
Summary: During early life, the development of the intestinal mucosa and immune system plays a crucial role in managing the gut microbiome and promoting tolerance towards commensal bacteria. The impact of maternal diet and microbial composition on offspring immune maturation remains poorly understood.
EMBO MOLECULAR MEDICINE
(2023)
Article
Cell Biology
M. Fernanda Palominos, Kathleen E. Whitlock
Summary: The study using zebrafish as a model system revealed that neutrophils and macrophages appear coincident with the generation of primitive immune cells in the early development of the olfactory organ. Damage to the olfactory sensory neurons triggers a rapid immune response by local and non-local neutrophils in larval zebrafish, while macrophages mount a slower response. This research may lead to new avenues of investigation into the role of olfactory-immune response in neurogenesis and regeneration, contributing to our understanding of potential host defense immune niches in the peripheral nervous system.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agricultural Engineering
Nevien Elhawat, Jozsef Csajbok, Szilvia Kovacs, Szilvia Veres, Miklos G. Fari, Eva Domokos-Szabolcsy, Tarek Alshaal
Summary: This study aimed to determine the appropriate phosphorus (P) fertilization management in the early development stage of giant reed (Arundo donax L.). Results showed that the concentration of applied P significantly affected P uptake, transportation, and use efficiency in giant reed. Different eco-types of giant reed exhibited variations in P accumulation and use efficiency, with the BL and STM eco-types performing the best.
BIOMASS & BIOENERGY
(2023)
Article
Fisheries
Xiaoting Gong, Min Li, Lili Zhang, Shiyu Huang, Guodong Wang
Summary: In this study, the MyD88 gene from Haliotis diversicolor was identified and its expression patterns during early embryonic development were analyzed. The results showed that MyD88 exhibited constitutive expression throughout all developmental stages, with the highest expression level at the 8 cell stage. Furthermore, knockdown of MyD88 resulted in a significant decrease in its expression, indicating its importance in the early innate immunity of abalone larvae.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Neurosciences
Tomoko Tanaka, Shinobu Hirai, Masato Hosokawa, Takashi Saito, Hiroshi Sakuma, Takaomi Saido, Masato Hasegawa, Haruo Okado
Summary: The study found that maternal separation can induce early AD-related pathology by affecting microglial activation and the vascular system.
EXPERIMENTAL NEUROLOGY
(2021)
Review
Immunology
Cristina Kalbermatter, Nerea Fernandez Trigo, Sandro Christensen, Stephanie C. Ganal-Vonarburg
Summary: The innate immune system is the oldest protection strategy conserved across all organisms, providing a fast defense mechanism against pathogens. The interaction between maternal immune cells, antibodies, dietary antigens, and microbial metabolites is crucial for preparing the newborn's immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Applied
Ekaterina Y. Melikhova, Nolwenn Derrien, Nadia Fleary-Roberts, Sian M. Forsyth, Sofia Papadouli, Antonio M. Ruda, Vargini G. Thangavadivale, Simon N. G. Tyler, Jonathan D. Moseley
Summary: The manufacturing route for a novel Parkinson's drug candidate (MCL-509) has been successfully scaled up from a small laboratory scale to a larger 20-50 L scale. The synthetic sequence remained unchanged, but significant improvements were made to all six reaction steps to accommodate the larger scale. Hazardous and toxic reagents were removed, concentrations and purifications were simplified, and cycle times were shortened. Furthermore, steps were combined and redeveloped with alternative reagents for better yield and purity. The manufacturing campaign performed as expected, delivering a 10-fold increase in material yield and improved quality.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Yoshihisa Kashima, Naoto Nishii, Hidetake Tachinami, Emi Furusawa, Shigenori Nagai, Hiroyuki Harada, Miyuki Azuma
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Toshihiro Yonekawa, Hidetoshi Watanabe, Norimitsu Hosaka, Shohei Semba, Atsushi Shoji, Masaki Sato, Masato Hamasaki, Shota Yuki, Shiori Sano, Yuji Segawa, Tsugunori Notomi
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Takeshi Azuma, Yujiro Sato, Tatsukuni Ohno, Miyuki Azuma, Haruki Kume
Article
Immunology
Xiang Ao, Yue Yang, Takashi Okiji, Miyuki Azuma, Shigenori Nagai
Summary: The study found that MDCs induced during sepsis contribute to immune paralysis, and the PD-1/PD-L1-mediated generation of Gr-1(hi) cells in the early phase of sepsis is crucial for the development of immune paralysis in the later stage.
INFECTION AND IMMUNITY
(2021)
Article
Hematology
Tomohiro Morio, Kenji Gotoh, Tomoyuki Imagawa, Kimio Morita, Hidenori Ohnishi, Kozo Yasui, Jutta Hofmann, John Philip Lawo, Amgad Shebl, Mikhail A. Rojavin
Summary: This study evaluated the safety and tolerability of IgPro10 in Japanese patients with PID and found that it was well tolerated, with consistent safety findings compared to studies in non-Japanese patients with PID.
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2021)
Article
Biology
Tomohiro Takehara, Ei Wakamatsu, Hiroaki Machiyama, Wataru Nishi, Katsura Emoto, Miyuki Azuma, Kenzo Soejima, Koichi Fukunaga, Tadashi Yokosuka
Summary: PD-1 forms microclusters with PD-L1/2 to inhibit T cell activation, with PD-L2 competing with PD-L1 for binding to PD-1. The cluster formation affects T cell signaling and serves as a visual index for the effectiveness of PD-1/2 inhibitory therapy.
COMMUNICATIONS BIOLOGY
(2021)
Editorial Material
Immunology
Motoi Yamashita, Tomohiro Morio
JOURNAL OF CLINICAL IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Teppei Ohkawa, Akira Nishimura, Kenjiro Kosaki, Yuki Aoki-Nogami, Daisuke Tomizawa, Kenichi Kashimada, Tomohiro Morio, Motohiro Kato, Shuki Mizutani, Masatoshi Takagi
Summary: A patient with intellectual disability and dysmorphic facial features developed PAX3/7-FOXO1 fusion-negative alveolar rhabdomyosarcoma, while also being diagnosed with Schuurs-Hoeijmakers syndrome.
JOURNAL OF HUMAN GENETICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Keeratika Wongtim, Eri Ikeda, Tatsukuni Ohno, Shigenori Nagai, Shigeru Okuhara, Keitetsu Kure, Miyuki Azuma
Summary: The study found that overexpression of PD-L1 in gingival basal keratinocytes in mice reduced periodontal inflammation and alveolar bone resorption in a ligature-induced periodontitis model.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Masato Hamasaki, Norimitsu Hosaka, Lita A. Freeman, Masaki Sato, Kazuo Hara, Alan T. Remaley, Kazuhiko Kotani
Summary: A novel LAMP-based genotyping method was developed and successfully applied to detect PCSK9 variants in FH, demonstrating relative convenience and efficiency compared to conventional sequencing methodologies. Future research on various genes is warranted for further validation and application in clinical settings.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Biochemical Research Methods
Yuka Nagatake, Masaki Sato, Yuta Mouri, Norihiro Tomita
Summary: In this study, a fully automated miRNA quantification method based on BLEIA was developed, which showed a wide linear range, high sensitivity, high specificity, and good reproducibility. It also demonstrated a significant correlation with quantitative reverse transcription-polymerase chain reaction.
ANALYTICAL BIOCHEMISTRY
(2022)
Review
Immunology
Kunihiko Moriya, Kanako Mitsui-Sekinaka, Yujin Sekinaka, Akifumi Endo, Hirokazu Kanegane, Tomohiro Morio, Kohsuke Imai, Shigeaki Nonoyama
Summary: Activated phosphatidyl inositol 3-kinase-delta syndrome (APDS) is caused by gain-of-function variant in the class IA PI3K catalytic subunit p110 delta. The disease is characterized by recurrent airway infections and bronchiectasis. It is associated with hyper-IgM syndrome, immune dysregulations, and T-cell dysfunction. Different causative genes lead to different types of APDS with varying severity levels. Proper treatment and management are crucial for patients with APDS.
IMMUNOLOGICAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Masaki Sato, Edward B. Neufeld, Martin P. Playford, Yu Lei, Alexander V. Sorokin, Angel M. Aponte, Lita A. Freeman, Scott M. Gordon, Amit K. Dey, Kianoush Jeiran, Masato Hamasaki, Maureen L. Sampson, Robert D. Shamburek, Jingrong Tang, Marcus Y. Chen, Kazuhiko Kotani, Josephine L. C. Anderson, Robin P. F. Dullaart, Nehal N. Mehta, Uwe J. F. Tietge, Alan T. Remaley
Summary: This study developed an HDL-specific phospholipid efflux (HDL-SPE) assay and found a strong association between HDL-SPE and coronary artery disease (CAD) as well as cardiovascular disease (CVD) events. Additionally, HDL-SPE was able to independently predict incident CVD events, regardless of traditional risk factors. Therefore, HDL-SPE could potentially serve as a routine clinical assay for improving CVD risk assessment and drug discovery.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Review
Immunology
Motoi Yamashita, Kento Inoue, Tsubasa Okano, Tomohiro Morio
Summary: Primary Immunodeficiency (PID) is a genetic disorder affecting the immune system, while Inborn Errors of Immunity (IEI) encompasses a broader range of immune dysfunction disorders. Advances in genetic analysis have aided in identifying disease-causing gene mutations, with treatments for IEI including hematopoietic cell transplantation and gene therapy. Further research on IEI is expected to provide insights into other immune-related disorders.
INFLAMMATION AND REGENERATION
(2021)
Article
Immunology
Yue Yang, Shigenori Nagai, Siwen Kang, Yulong Xia, Yohei Kawano, Kensuke Miyake, Hajime Karasuyama, Miyuki Azuma
INTERNATIONAL IMMUNOLOGY
(2020)