Review
Biotechnology & Applied Microbiology
Peter Setlow, Graham Christie
Summary: Spores can spread food spoilage, diseases, and intoxications, and methods for spore killing include heat, radiation, high pressure, and chemicals. Factors to consider in choosing methods for spore killing include cost, efficacy, operation scope, and compatibility.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Microbiology
Anella Saggese, Giovanni Di Gregorio Barletta, Maria Vittoria, Giuliana Donadio, Rachele Isticato, Loredana Baccigalupi, Ezio Ricca
Summary: The CotG protein in the Bacillus subtilis spore coat controls spore permeability through its central region of positively charged tandem repeats. The presence of central repeats is a common feature in CotG-like proteins in most Bacillus species. This study contributes to the understanding of the structure and function of bacterial spores.
Article
Biochemistry & Molecular Biology
Giannina Espina, Paulina Caceres-Moreno, Guillermo Mejias-Navarrete, Minghua Ji, Junsong Sun, Jenny M. Blamey
Summary: Laccases are important enzymes with wide substrate specificity and biotechnological potential. This research presents a novel spore-coat laccase with high specific activity, broad temperature range, and the ability to rapidly decolorize various synthetic dyes at 40 degrees Celsius.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Noemie Verguet, Lou Mondange, Flora Nolent, Anne Depeille, Annabelle Garnier, Fabienne Neulat-Ripoll, Olivier Gorge, Jean -Nicolas Tournier
Summary: Contamination with microorganisms is a concern in laboratories and bioterrorism. A calcium hypochlorite-based decontaminant was found to be effective in killing Bacillus anthracis spores and complied with laboratory standards. It achieved complete disinfection within 1 minute for spillage and 2 minutes for routine use.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Biophysics
Michal Bodik, Daniela Krajcikova, Jakub Hagara, Eva Majkova, Imrich Barak, Peter Siffalovic
Summary: The Bacillus subtilis spore coat is a bacterial proteinaceous structure with unique characteristics such as self-organization, toughness, and flexibility. It is composed of over 80 coat proteins, some of which form two-dimensional crystal structures. By applying Grazing-Incidence Wide-Angle X-ray Scattering (GIWAXS), researchers were able to investigate the diffraction pattern of CotY 2D crystals formed on Langmuir monolayer films. The diffractions obtained pave the way for determining the 3D structure of coat proteins using strong X-ray sources.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Microbiology
Megan H. Touchette, Hector Benito de la Puebla, Carolina Alves Feliciano, Benjamin Tanenbaum, Monica Schenone, Steven A. Carr, Aimee Shen
Summary: In this study, a new spore morphogenetic factor specific to Clostridioides difficile, SpoVQ, was identified. SpoVQ acts in conjunction with known spore coat morphogenetic factors, SpoIVA and SipL, to regulate the formation of the protective spore cortex layer in C. difficile. This discovery provides a novel pathway that could potentially be targeted to prevent the formation of infectious C. difficile spores.
Article
Chemistry, Analytical
Xin Shu, Qing-Qing Liao, Shang-Tong Li, Lu Liu, Xiajun Zhang, Lianqi Zhou, Long Zhang, Irene Coin, Lei Wang, Haifan Wu, Bing Yang
Summary: Post-translational modification of proteins by Ubiquitin (Ub) and Ubiquitin-like proteins (Ubls) can be reversed by deconjugating enzymes. Developing cell-permeable Ub/Ubl probes for live cell applications is challenging. Here, genetically encoded cross-linkable Ub/Ubl probes were developed to investigate deconjugating enzyme activity in physiological environments.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Daniela Krajcikova, Veronika Bugarova, Imrich Barak
Summary: This study investigates the interactions of key proteins involved in Bacillus subtilis endospore coat and cortex formation, revealing a complex network of interactions and the crucial role of protein cooperation in spore resistance and germination.
Article
Multidisciplinary Sciences
Patrick A. Sandoz, Kyra Kuhnigk, Edina K. Szabo, Sarah Thunberg, Elina Erikson, Niklas Sandstroem, Quentin Verron, Andreas Brech, Carsten Watzl, Arnika K. Wagner, Evren Alici, Karl-Johan Malmberg, Michael Uhlin, Bjoern Oenfelt
Summary: Gamma delta T cells play a vital role in protecting against infections and tumors throughout life. Their cytotoxic functions are regulated by the availability of lytic molecules granzyme B and perforin. Live single-cell imaging provides new insights into the cytotoxic arsenal and functions of gamma delta T cells, which may aid in the development of more effective immunotherapies.
NATURE COMMUNICATIONS
(2023)
Review
Food Science & Technology
Alessia I. Delbruck, Yifan Zhang, Rosa Heydenreich, Alexander Mathys
Summary: Spore-forming bacteria are resistant to stress conditions due to their ability to form highly resistant dormant spores, which can survive adverse environmental conditions and decontamination processes. Pressure-mediated spore germination is heterogeneous, with some spores germinating rapidly while others exhibit slow or no germination. Understanding the mechanisms of HP-mediated germination is crucial for the successful implementation of germination-inactivation strategies in food industry applications.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2021)
Article
Environmental Sciences
Joseph Wood, Abderrahmane Touati, Ahmed Abdel-Hady, Denise Aslett, Francis Delafield, Worth Calfee, Erin Silvestri, Shannon Serre, Leroy Mickelsen, Christine Tomlinson, Anne Mikelonis
Summary: The study demonstrates the feasibility of using dry thermal techniques to decontaminate soils contaminated with Bacillus anthracis spores. Results showed that increasing temperature and relative humidity reduced the time required to achieve soil samples with no spores detected. The thermal inactivation of spores varied depending on the temperature and humidity conditions, with different depth and soil types impacting the effectiveness of the treatment.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Virology
Matthew Ykema, Kai Ye, Meng Xun, Justin Harper, Miguel A. A. Betancourt-Solis, Carlos F. F. Arias, James A. A. McNew, Yizhi Jane Tao
Summary: The human astrovirus (HAstV) is a common cause of gastroenteritis, and its cell entry mechanism and membrane penetration activity have been investigated in this study. It was found that the capsid protein of HAstV has a trypsin-dependent lipid disruption activity, essential for virus infectivity. This discovery provides valuable insights into HAstV pathogenesis and offers a potential target for therapeutics.
JOURNAL OF VIROLOGY
(2023)
Article
Agronomy
Xuelian Li, Yanli Zhang, Yunda Zhan, Hongwei Tian, Bing Yan, Jun Cai
Summary: A new promoter P-rsi from Bacillus thuringiensis was discovered to allow abundant expression of vip3Aa, and by knocking out protease genes, the degradation of vip3Aa was greatly inhibited and its yield was significantly improved. These findings have reference value for the research and development of novel bioinsecticides.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xiaowei Gao, Bhagyashree N. Swarge, Henk L. Dekker, Winfried Roseboom, Stanley Brul, Gertjan Kramer
Summary: The study utilized a membrane enrichment method to analyze the membrane proteome of Bacillus cereus spores and cells, identifying specific protein compositions in the spore inner membrane and cellular membrane. Results showed that the spore inner membrane expressed germinant receptors and spore-specific proteins, while the cellular membrane had a higher presence of transporters, receptors, and proteins related to cell division and motility. These findings demonstrate the differences in membrane proteome composition between spores and cells of the toxigenic spore-forming bacterium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Michael Y. Galperin, Natalya Yutin, Yuri Wolf, Roberto Vera Alvarez, Eugene Koonin
Summary: Many members of the phylum Firmicutes (Bacillota) are capable of producing endospores, which enhance the survival of important Gram-positive pathogens. The core set of sporulation genes is conserved in a wide variety of sporeformers from several distinct lineages of Firmicutes. Widespread loss of sporulation genes was detected in many organisms, particularly within the families Planococcaceae and Erysipelotrichaceae. These findings provide insights into the study of sporulation mechanisms.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xia Wu, Navdeep Grover, Elena E. Paskaleva, Ruchir V. Mundra, Martin A. Page, Ravi S. Kane, Jonathan S. Dordick
BIOTECHNOLOGY AND BIOENGINEERING
(2015)
Article
Biotechnology & Applied Microbiology
Elena E. Paskaleva, Ruchir V. Mundra, Krunal K. Mehta, Ravindra C. Pangule, Xia Wu, Willing S. Glatfelter, Zijing Chen, Jonathan S. Dordick, Ravi S. Kane
BIOTECHNOLOGY PROGRESS
(2015)
Article
Biochemistry & Molecular Biology
Jicong Cao, Manish Arha, Chaitanya Sudrik, Abhirup Mukherjee, Xia Wu, Ravi S. Kane
NUCLEIC ACIDS RESEARCH
(2015)
Article
Biotechnology & Applied Microbiology
Krunal K. Mehta, Elena E. Paskaleva, Xia Wu, Navdeep Grover, Ruchir V. Mundra, Kevin Chen, Yongrong Zhang, Zhiyong Yang, Hanping Feng, Jonathan S. Dordick, Ravi S. Kane
BIOTECHNOLOGY AND BIOENGINEERING
(2016)
Review
Chemistry, Applied
Xia Wu, Seok-Joon Kwon, Jungbae Kim, Ravi S. Kane, Jonathan S. Dordick
ANNUAL REVIEW OF CHEMICAL AND BIOMOLECULAR ENGINEERING, VOL 8
(2017)
Article
Nanoscience & Nanotechnology
Domyoung Kim, Seok-Joon Kwon, Xia Wu, Jessica Sauve, Inseon Lee, Jahyun Nam, Jungbae Kim, Jonathan S. Dordick
ACS APPLIED MATERIALS & INTERFACES
(2018)
Article
Biotechnology & Applied Microbiology
Xia Wu, Seok Joon Kwon, Domyoung Kim, Jian Zha, Mauricio Mora-Pale, Jonathan S. Dordick
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Jian Zha, Ying Zang, Matthew Mattozzi, Jens Plassmeier, Mamta Gupta, Xia Wu, Sonya Clarkson, Mattheos A. G. Koffas
MICROBIAL CELL FACTORIES
(2018)
Article
Multidisciplinary Sciences
Xia Wu, Elena E. Paskaleva, Krunal K. Mehta, Jonathan S. Dordick, Ravi S. Kane
SCIENTIFIC REPORTS
(2016)
Article
Nanoscience & Nanotechnology
Xia Wu, Keith Fraser, Jian Zha, Jonathan S. Dordick
ACS APPLIED MATERIALS & INTERFACES
(2018)
Article
Agriculture, Multidisciplinary
Ying Zang, Jian Zha, Xia Wu, Zhaojuan Zheng, Jia Ouyang, Mattheos A. G. Koffas
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Xia Wu, Jian Zha, Mattheos A. G. Koffas, Jonathan S. Dordick
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Review
Biochemical Research Methods
Jian Zha, Xia Wu, Mattheos A. G. Koffas
CURRENT OPINION IN BIOTECHNOLOGY
(2020)