Article
Biochemistry & Molecular Biology
Xiaohan Ye, Tianwen Zheng, Wenming Zhang, Weiliang Dong, Hao Wu, Jiangfeng Ma, Min Jiang
Summary: This study developed membrane engineering strategies to improve the transmembrane transport of electrons during cathodic electro-fermentation, leading to an increase in total electron quantity and succinate yield.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Rana Deeba, Sylvie Chardon-Noblat, Cyrille Costentin
Summary: The study investigates homogeneous electrochemical catalysis of N2O reduction to N-2 using various organic catalysts and rhenium and manganese bipyridyl carbonyl complexes. It reveals an activation-driving force correlation with the organic species characteristic of a redox catalysis involving an outer-sphere electron transfer. The analysis shows that the catalytic effect of the mediators is due to a smaller reorganization energy of the homogeneous electron transfer compared to the heterogeneous process.
Article
Environmental Sciences
Jingxuan Wu, Yunfei Li, Xuepeng Chen, Nan Li, Weihua He, Yujie Feng, Jia Liu
Summary: By adjusting membrane permeability with the use of cetyltrimethylammonium bromide (CTAB), microbial fuel cell (MFC) performance can be enhanced, promoting extracellular electron transfer (EET) and improving the efficiency of substrate transport.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Plant Sciences
Kentaro Usui, Haruki Yamamoto, Takao Oi, Mitsutaka Taniguchi, Hitoshi Mori, Yuichi Fujita
Summary: This study demonstrated that extracellular vesicles mediate the secretion of Pchlide in YFC2 cells grown in the dark. Pchlide fractions were isolated using sucrose density gradient centrifugation, and mass spectrometry analysis revealed the presence of porin isoforms, TolC, and FG-GAP repeat-containing protein in the fractions, which are localized in the outer membrane.
Article
Microbiology
Sota Ihara, Satoshi Wakai, Tomoko Maehara, Akihiro Okamoto
Summary: This study successfully cultured ultramicrobacteria that passed through a 0.22 μm filter from soil samples and isolated a novel species called Cellulomonas sp. strain NTE-D12. Electrochemical and genomic analyses showed that the strain NTE-D12 had a unique electron transfer system and generated the highest current density.
Review
Biotechnology & Applied Microbiology
Xia Wu, Jingyi Liu, Zhiqiang Liu, Guoli Gong, Jian Zha
Summary: This article summarizes the important role of microbial cell surface layers in microbial engineering, introduces the strategies and methods of metabolic engineering using microbial cell surface components, and discusses the existing problems and future directions.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biology
Daniel A. Phillips, Lori A. Zacharoff, Cheri M. Hampton, Grace W. Chong, Anthony P. Malanoski, Lauren Ann Metskas, Shuai Xu, Lina J. Bird, Brian J. Eddie, Aleksandr E. Miklos, Grant J. Jensen, Lawrence F. Drummy, Mohamed Y. El-Naggar, Sarah M. Glaven
Summary: BAR domain proteins are a superfamily of coiled-coil proteins that influence membrane curvature in eukaryotes, and a bacterial protein BdpA with BAR domain-like activity has been discovered to impact the formation of membrane vesicles and outer membrane extensions. BdpA is essential for the uniform size distribution of membrane vesicles and the scaffolding of consistent diameter and curvature in OMEs, influencing the morphology of these structures. Overexpression of BdpA can promote the formation of OMEs in bacterial species where it is not typically observed, suggesting a new class of bacterial BAR domain-like proteins.
Review
Biotechnology & Applied Microbiology
Baocai Zhang, Sicheng Shi, Rui Tang, Chunxiao Qiao, Meiyi Yang, Zixuan You, Shulin Shao, Deguang Wu, Huan Yu, Junqi Zhang, Yingxiu Cao, Feng Li, Hao Song
Summary: This review provides a systematic summary of screening technologies for exoelectrogenic microorganisms (EEMs), including enrichment, isolation, and bio-electrochemical activity evaluation. It first summarizes the distribution characteristics of known EEMs and then explains the EET mechanisms and principles underlying various technological approaches. Finally, it presents a future perspective on EEM screening and bio-electrochemical activity evaluation, focusing on developing new EET mechanisms for the next-generation EEM screening technologies and exploring nonculturable EEMs using meta-omics approaches and bioinformatics analyses.
BIOTECHNOLOGY ADVANCES
(2023)
Review
Physiology
Maria Carmela Di Rosa, Francesca Guarino, Stefano Conti Nibali, Andrea Magri, Vito De Pinto
Summary: Mitochondrial porins, also known as VDACs, are molecules forming pores in the outer mitochondrial membranes, regulating metabolic flux between cytosol and mitochondria. The specific functions of different isoforms, especially in yeast cells, are still not well understood, despite the evolutionary conservation of VDAC proteins across species.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Engineering, Environmental
Vaanie Godbole, Simranjeet Singh, Praveen C. Ramamurthy, Nadeem A. Khan, Manisha Bisht, Manoj Kumar Pal, Joginder Singh, Gaurav Kumar, Ali Esrafili, Mahmood Yousefi
Summary: The rising environmental issues have led to increased research in microbial fuel cells (MFCs) as an eco-friendly and sustainable energy source. The discovery of electroactive bacteria (EABs) and their role in MFCs has significantly improved energy generation by facilitating electron transfer to the anode. EABs can perform extracellular electron transfer (EET), where microbes utilize substrates for growth and transfer the generated electrons to the anode for energy production. EET occurs through direct pathways involving physical contact of bacteria with the anode using outer membrane cytochromes or conductive pili, and indirect pathways involving endogenous or exogenous mediators. Understanding these EET pathways is crucial for utilizing EABs and MFCs in practical applications.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Jian Wang, Chuang Hao, Lei Cao, Yitong Yao, Yidi Ding, Yong Yang, Xiao-Feng Tang, Bing Tang
Summary: Escherichia coli is commonly used for recombinant protein production, but its limited capacity for producing extracellular proteins is seen as a drawback. A study found that NJ7G_0991, an extracellular protein from the haloarchaeon Natrinema sp. J7-2, can be efficiently released into the culture supernatant of E. coli. By fusing a PelB signal peptide to the N-terminus of the LolA-like domain, the extracellular production level of recombinant proteins can be significantly enhanced.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Xian-Zhong Fu, Jie Wu, Shuo Cui, Xue-Meng Wang, Hou-Qi Liu, Ru-Li He, Cheng Yang, Xin Deng, Zhou-Liang Tan, Wen-Wei Li
Summary: A self-regenerable bio-hybrid composed of bacterial cells and bio-FeS was developed for efficient removal of chromium, maintaining high activity within consecutive treatment-regeneration cycles. This innovative approach offers a promising new avenue for sustainable treatment of heavy metal-containing wastewater.
Article
Cell Biology
Jialin Zhou, Martin Jung, Kai S. Dimmer, Doron Rapaport
Summary: In this study, we investigated the biogenesis of multi-span proteins in the mitochondrial outer membrane. Our findings suggest that the targeting specificity and efficiency of these proteins are influenced by their length, as well as the behavior of the membrane. Additionally, cytosolic proteins do not significantly contribute to the biogenesis process.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Lin Mei, Zhenlong Xu, Zhiqiang Miao, Mengyao Yun, Yidan Luan, Dehong Yang, Luxi Xia
Summary: This study proposed a new photosensitive antibacterial agent Pc-PB, which combines the photodynamic antibacterial activity of phthalocyanine with the antibacterial ability of polymyxin B, to accelerate the healing of bacteria-infected wounds. The results showed that Pc-PB has bactericidal effects on both Gram-positive and Gram-negative bacteria, and demonstrates good biocompatibility.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Environmental Sciences
Yifei Wang, Yaohuan Gao, Abid Hussain, Hyung-Sool Lee
Summary: This study aimed to develop a standardized and consistent method for measuring biofilm conductance to enhance the understanding of extracellular electron transfer. The results showed no significant difference in biofilm conductance with and without a fixed anode potential. The conductance exhibited a sigmoidal relationship with anode potential and deviated from Ohm's law at applied voltages larger than 100 mV. Increasing measurement time led to a decrease in biofilm conductance and further deviation from Ohm's law. By optimizing operation settings, such as voltage range, step, measurement time, and anode control mode, more accurate and consistent biofilm conductance measurement was achieved in the 2-Au MEC system. This study provided a comprehensive evaluation of environmental and instrumental parameters for biofilm conductance measurement.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Yidan Hu, Yun Yang, Evgeny Katz, Hao Song
CHEMICAL COMMUNICATIONS
(2015)
Article
Chemistry, Physical
Yun Yang, Yichao Wu, Yidan Hu, Yingxiu Cao, Chueh Loo Poh, Bin Cao, Hao Song
Article
Biochemical Research Methods
Yun Yang, Yuanzhao Ding, Yidan Hu, Bin Cao, Scott A. Rice, Staffan Kjelleberg, Hao Song
ACS SYNTHETIC BIOLOGY
(2015)
Article
Chemistry, Multidisciplinary
Hua Wang, Fengshi Li, Bowen Zhu, Lin Guo, Yun Yang, Rui Hao, Hong Wang, Yaqing Liu, Wei Wang, Xintong Guo, Xiaodong Chen
ADVANCED FUNCTIONAL MATERIALS
(2016)
Article
Chemistry, Multidisciplinary
Pengfei Hu, Hua Wang, Yun Yang, Jie Yang, Jie Lin, Lin Guo
ADVANCED MATERIALS
(2016)
Article
Chemistry, Multidisciplinary
Yun Yang, Hua Wang, Rui Hao, Lin Guo
Review
Chemistry, Physical
Hua Wang, Yun Yang, Lin Guo
ADVANCED ENERGY MATERIALS
(2017)
Article
Chemistry, Multidisciplinary
Jing Zhou, Caihong Zheng, Yun Yang, Lin Guo
Article
Chemistry, Multidisciplinary
Pengfei Hu, Tinghan Chen, Yun Yang, Hua Wang, Zihao Luo, Jie Yang, Haoran Fu, Lin Guo
Article
Nanoscience & Nanotechnology
Juanjuan Qi, Ke Chen, Shuhao Zhang, Yun Yang, Lin Guo, Shihe Yang
Article
Chemistry, Multidisciplinary
Hua Wang, Bowen Zhu, Wencao Jiang, Yun Yang, Wan Ru Leow, Hong Wang, Xiaodong Chen
ADVANCED MATERIALS
(2014)
Article
Chemistry, Multidisciplinary
Yang-Chun Yong, Yang-Yang Yu, Xinhai Zhang, Hao Song
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2014)
Review
Chemistry, Multidisciplinary
Hao Song, Ming-Zhu Ding, Xiao-Qiang Jia, Qian Ma, Ying-Jin Yuan
CHEMICAL SOCIETY REVIEWS
(2014)
Article
Biotechnology & Applied Microbiology
Jian Zha, Minghua Shen, Menglong Hu, Hao Song, Yingjin Yuan
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2014)
Article
Biophysics
Gaolong Zhu, Yun Yang, Juan Liu, Feng Liu, Anhuai Lu, Weidong He
BIOSENSORS & BIOELECTRONICS
(2017)