Article
Biochemical Research Methods
Sergio M. Vilas-Boas, Isabella W. Cordova, Kiki A. Kurnia, Heloisa H. S. Almeida, Priscilla S. Gaschi, Joao A. P. Coutinho, Simao P. Pinho, Olga Ferreira
Summary: Countercurrent and centrifugal partition chromatography are techniques for separating compounds from natural extracts, with the selection of the biphasic solvent system being a key design parameter. COSMO-RS and NRTL-SAC models were used to estimate partition coefficients of phenolic compounds in different solvent systems, with the NRTL-SAC model showing higher reliability.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Engineering, Chemical
Ariane A. Oshiro, Alexandre M. S. Jorge, Valeria C. Santos-Ebinuma, Jorge F. B. Pereira
Summary: This study proposes an aqueous two-phase system (ATPS) composed of ethanol and dipotassium hydrogen phosphate for Centrifugal Partition Chromatography (CPC). Compared to polymer-based ATPS, the Ethanol/K2HPO4 system overcomes hydrodynamic drawbacks and demonstrates advantages such as short settling time, high interfacial tension, significant density differences, and low viscosity. CPC experiments show that controlling the operating parameters can achieve stable conditions and successful separation of biomolecules using the ethanol/salt ATPS.
SEPARATION AND PURIFICATION TECHNOLOGY
(2024)
Article
Biochemical Research Methods
Tim Hammerschick, Walter Vetter
Summary: Centrifugal partition chromatography (CPC) and countercurrent chromatography (CCC) are two preparative techniques used for the isolation and purification of natural products. This study successfully combined the advantages of both techniques by directly linking CPC and CCC, and demonstrated the effectiveness of the hybrid system.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Alvaro Silva Lima, Bruno Sales de Oliveira, Selesa Vanessa Shabudin, Mafalda Almeida, Mara Guadalupe Freire, Katharina Bica
Summary: This work aimed to integrate the extraction and purification of anthocyanins from grape pomace using ATPS and scaled-up CPC, with protic ionic liquids as the common solvent. Different operational conditions led to varied purification factors, with ATPS and CPC achieving significant purification of anthocyanins. The choice of optimal operational conditions depends on the specific application of anthocyanins.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Virology
Kang Ning, Anuradha Roy, Fang Cheng, Peng Xu, Steve Kleiboeker, Carlos R. Escalante, Jingxin Wang, Jianming Qiu
Summary: This study developed a high-throughput screening assay for identifying antivirals against B19V replication and discovered a purine derivative that exhibited significant antiviral activity against B19V infection.
JOURNAL OF VIROLOGY
(2022)
Article
Engineering, Chemical
Mafalda R. Almeida, Filipe Ferreira, Pedro Domingues, Joao A. P. Coutinho, Mara G. Freire
Summary: This study successfully developed a method to purify IgY from egg yolk, combining water-soluble protein fraction and aqueous biphasic systems, followed by centrifugal partition chromatography for purification. The operating conditions of CPC were optimized to obtain IgY with high purity.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Anne Pajot, Sebastien Chollet, Elodie Nicolau, Luc Marchal
Summary: This study aims to optimize the recovery and purity of fucoxanthin from Tisochrysis lutea using the CPC technique. CPC provides fast and room temperature extraction without degradation. By optimizing the solvent composition and operating conditions, nine fractions with 100% purity and 92% recovery yield were obtained.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Pratik U. Joshi, Stephanie M. Kroger, Silviya P. Zustiak, Caryn L. Heldt
Summary: Aqueous two-phase systems (ATPS) are used in bioseparations and microencapsulation. Biomolecule behavior at the interface between the two phases is not well understood. The study focused on using tie-lines to analyze biomolecule partitioning behavior. The results showed that the recovery of porcine parvovirus (PPV) was higher when PEG was the bulk phase, and a peptide conjugate with WRW ligand enhanced the recovery in a lower tie-line system.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Engineering, Chemical
Bruna P. Soares, Joao H. P. M. Santos, Margarida Martins, Mafalda R. Almeida, Nathalia Santos, Mara G. Freire, Valeria C. Santos-Ebinuma, Joao A. P. Coutinho, Jorge F. B. Pereira, Sonia P. M. Ventura
Summary: This study investigated the continuous purification of EGFP using ABS, with an initial screening conducted by changing the electrolyte content in the ABS to find the optimal conditions. The results showed a high purification rate (89.93%) and recovery yield (82.3%), indicating the potential of FCPC for the continuous purification of EGFP.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Magdalena Maciejewska-Turska, Lukasz Pecio, Grazyna Zgorka
Summary: Pueraria lobata is a legume plant native to Southeast Asia, widely used in traditional medicine. This study developed an original preparative method to efficiently isolate hydrophilic daidzein C-glycosides, including mirificin, from kudzu roots. The isolated mirificin showed a purity of 63% and exhibited significant anti-biodegenerative effects.
Article
Environmental Sciences
Swati Verma, Ki-Hyun Kim
Summary: Ground water contamination by radioactive elements is a critical issue with significant threats to human health. Adsorption, especially using graphene oxide and its derivatives, is recognized as a promising method for removal due to their unique 2D structure, high surface area, and intercalated functional groups. This article provides insights into the practical applicability, preparation, and functionalization of graphene-based adsorbents for uranium removal, with guidance for future research directions in this field.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Ana N. Nunes, Viktoriya Ivasiv, Luis F. Gouveia, Naiara Fernandez, Joana Oliveira, Maria Rosario Bronze, Ana A. Matias
Summary: Replacing synthetic colorants with natural ones is a popular marketing trend, but naturally occurring blue color is scarce. This study developed a more efficient and sustainable alternative method, centrifugal partition chromatography (CPC), for purifying portisins. The CPC process showed a higher efficiency and a lower environmental risk factor compared to reversed phase column chromatography (RP-CC). This research suggests that CPC is an effective and sustainable method for isolating portisins and utilizing blueberry surplus as a natural blue colorant.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Ji Hoon Kim, Je-Seung Jeon, Jung Hoon Kim, Eun Ju Jung, Yun Jung Lee, En Mei Gao, Ahmed Shah Syed, Rak Ho Son, Chul Young Kim
Summary: This study used CPC and HPLC techniques to separate and identify two eudesmane type sesquiterpenes with ARE-inducing activity from the crude extract of Lindera strychnifolia roots.
Article
Chemistry, Analytical
Fabian Hammerle, Michael Zwerger, Anja Hoeck, Markus Ganzera, Ursula Peintner, Bianka Siewert
Summary: Recently, it has been discovered that certain fungal pigments have significant photoactivity, making them promising candidates for treating microbial infections or malignant diseases. In this study, a separation method for fungal anthraquinones was developed using centrifugal partition chromatography. The method was successfully applied to purify multiple pigments from fungal extracts.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Jean-Baptiste Chagnoleau, Ines L. D. Ryan, Ryan Khedher, Joao AP Coutinho, Thomas Michel, Xavier Fernandez, Nicolas Papaiconomou
Summary: A study was conducted on ternary biphasic systems composed of heptane, 1-butanol or ethyl acetate, and type III or type V deep eutectic solvents based on levulinic acid and choline chloride or thymol. The binodal curves, densities, and phase compositions of the equilibrium phases were determined for seven systems. The partition coefficients of six natural compounds were measured in these systems. Based on the results, a system composed of heptane, 1-butanol, and the deep eutectic solvent choline chloride:levulinic acid at a molar ratio of 1:3 was selected for centrifugal partition chromatography and showed good separation of apigenin, coumarin, beta-ionone, and alpha-tocopherol.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biotechnology & Applied Microbiology
David Saleh, Gang Wang, Benedict Mueller, Federico Rischawy, Simon Kluters, Joey Studts, Juergen Hubbuch
Summary: The study successfully scaled up the anion exchange chromatography step using a multicomponent steric mass-action model; By comparing experimental results and modeling data, differences between scales can be identified early; A multi-stage model qualification approach was established to assess model quality and understand limitations across scales.
BIOTECHNOLOGY PROGRESS
(2021)
Article
Biotechnology & Applied Microbiology
Marieke E. Klijn, Jurgen Hubbuch
Summary: This study evaluated the impact of image analysis strategies and experimental parameters on T-Cloud,T-app detection, showing that total number of white pixels can detect T-Cloud,T-app and extract ice nucleation temperature. Variations in experimental parameters led to changes in T-Cloud,T-app values, but did not affect comparability between samples.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Dominik Stein, Volkmar Thom, Juergen Hubbuch
Summary: This paper presents a mechanistic model case study for the separation of two different two-component solutions, confirming prior evidence. A novel HTS screening procedure was developed, incorporating competitive adsorption-based displacement effects and key parameter identification. The screening procedure, using an overload bind and elute mode, was applied to a case study dealing with IgG aggregate removal, resulting in significant improvements in binding capacities.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Christina H. Wegner, Ines Zimmermann, Juergen Hubbuch
Summary: This study aimed to develop a low-volume, quantitative analytical tool based on UV-visible spectroscopy and partial least squares modeling for faster development of crystallization processes. The developed tool successfully quantified different species in the supernatant with high precision, achieving a sensitivity of 96.23% and a short analysis time of 3 minutes per sample.
CRYSTAL GROWTH & DESIGN
(2022)
Article
Biotechnology & Applied Microbiology
Narmin Suvarli, Lukas Wenger, Christophe Serra, Iris Perner-Nochta, Juergen Hubbuch, Michael Woerner
Summary: Increasing the shelf life and reusability of enzymes is a crucial topic in biotechnology. This study presents a method to immobilize beta-galactosidase on polymer nanoparticles and then encapsulate them inside hydrogel microparticles using a microfluidic device and UV-LEDs. The encapsulation in acrylamide microparticles caused a loss of enzymatic activity, while encapsulation in PEG-diacrylate microparticles preserved some activity. Storage at -26 degrees C resulted in a complete loss of enzymatic activity. The presence of a photoinitiator exposed to UV-irradiation also influenced the enzyme activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Chemical
Till Briskot, Nils Hillebrandt, Simon Kluters, Gang Wang, Joey Studts, Tobias Hahn, Thiemo Huuk, Juergen Hubbuch
Summary: The Gibbs-Donnan effect causes uneven distribution of ions across semipermeable membranes, leading to discrepancies in pH and excipient concentrations in protein ultrafiltration and diafiltration processes. This study introduces a model that describes the composition of retentate and permeate in combined UF/DF processes, taking into account volume exclusion effects and electrostatic interactions between ions and proteins.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Lukas Wenger, Juergen Hubbuch
Summary: Hydrogels, polymer-based materials with a high water content, are widely used in biotechnological applications due to their biocompatibility and cell-friendly nature. This study presents an approach to estimate diffusion coefficients in hydrogels using absorbance measurements of a UV area imaging system.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Svenja Strauss, Bianca Schroth, Juergen Hubbuch
Summary: Bioprinting is considered a suitable additive manufacturing method in biopharmaceutical process development. To achieve industrial application, higher levels of reproducibility and a standardized bioprinting process are necessary. This study investigates the effect of different processing modes (set pressure vs. set flow rate) on printing reproducibility. The results show that a set flow rate controlled printing process improves accuracy and the filling level also affects the accuracy of printing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Federico Rischawy, Till Briskot, Adrian Schimek, Gang Wang, David Saleh, Simon Kluters, Joey Studts, Juergen Hubbuch
Summary: A fundamental process understanding of an entire downstream process is essential for achieving and maintaining high-quality standards demanded for biopharmaceutical drugs. A holistic process model based on mechanistic insights could support process development by identifying dependencies between process parameters and critical quality attributes across unit operations to design a holistic control strategy.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biotechnology & Applied Microbiology
David Saleh, Rudger Hess, Michelle Ahlers-Hesse, Federico Rischawy, Gang Wang, Jan-Hendrik Grosch, Thomas Schwab, Simon Kluters, Joey Studts, Juergen Hubbuch
Summary: The development of downstream processes in biopharmaceuticals relies on experimental studies, but the relationship between the protein structure of mAbs and their macroscopic process behavior is poorly understood. This study introduces a multiscale in silico model that allows the development of chromatography processes before protein material is available for experimental studies. The model leverages the mAbs' structural characteristics and experimental data to predict elution profiles and improve the development of chromatography processes for novel antibodies.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Sandra Haas, Saskia Koerner, Laura Zintel, Juergen Hubbuch
Summary: Hydrogels based on renewable resources are promising materials for pharmaceuticals, drug delivery, and personalized medicine. The mechanical properties of these hydrogels, such as storage modulus and elasticity, can be adjusted by changing the accessibility of tyrosine residues and the composition of buffer systems and protein concentration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Laura Rolinger, Juergen Hubbuch, Matthias Ruedt
Summary: Monitoring the protein concentration and buffer composition during UF/DF step is crucial for biopharmaceutical production automation and real-time release testing. This study demonstrates the advantages of combining Raman spectroscopy with EKF for monitoring the UF/DF step, showing improved sensitivity and faster measurement speed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Robin Schiemer, Jan Tobias Weggen, Katrin Marianne Schmitt, Juergen Hubbuch
Summary: In the production of antibody-drug conjugates (ADCs), real-time monitoring of the conjugation reaction is crucial for ensuring product safety and efficacy. This study successfully combined a sensor model and a kinetic reaction model using an extended Kalman filter (EKF), which improved the accuracy of predictions and enhanced robustness against experimental noise or model errors. The developed framework provides a reliable method for monitoring ADC conjugation reactions and has the potential to be implemented in process control and applied to other ADC conjugation reaction types.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemical Research Methods
Rudger Hess, Jan Faessler, Doil Yun, David Saleh, Jan-Hendrik Grosch, Thomas Schwab, Juergen Hubbuch
Summary: This study introduces a method for predicting the elution behavior of antibodies in multimodal chromatography based on their amino acid sequences. By analyzing the data of 64 full-length antibodies, the study identifies key structural features and establishes a predictive model for pH gradient elution. The developed model can inform process development and enable in silico manufacturability assessment for target antibodies adhering to standardized purification conditions.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)