Article
Biochemistry & Molecular Biology
Jon Ashley, Anna-Lisa Schaap-Johansen, Mohsen Mohammadniaei, Maryam Naseri, Paolo Marcatili, Marta Prado, Yi Sun
Summary: The TdT enzyme plays a crucial role in V(D)J recombination in lymphocytes, allowing for diverse expression of immunoglobulins and T-cell receptors. A new screening method was demonstrated using TdT to form vsDNA libraries for binding to protein targets, offering the possibility of rational aptamer design and controlled reaction conditions for improved screening outcomes. This approach allows for screening based on both size and sequence in a single round, reducing PCR bias and resulting in high affinity and selective aptamers.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Spectroscopy
Zhimei Li, Ting Pi, Kefang Yang, Ziyi Xia, Yuchuan Feng, Xiangjuan Zheng, Ruihong Deng, Baozhu Chi
Summary: A label-free fluorescence probe based on poly-thymine copper nanoclusters engineered by terminal deoxynucleotidyl transferase is proposed for methyltransferase activity assay. This economical method demonstrates specificity and high efficiency for screening DNA methylation inhibitors, showing great potential for future drug development and clinical cancer diagnosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemical Research Methods
Yue Sun, Liu Zang, Jianzhong Lu
Summary: In this study, a sensitive method was developed to simultaneously detect multiple DNA glycosylases. The method is based on the target-initiated removal of damaged base and terminal deoxynucleotidyl transferase (TdT)-assisted labeling and signal amplification. The method has the advantages of high sensitivity, specificity, and multiplex and can be used for DNA glycosylase-related biomedical research.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Spectroscopy
Xueling Shang, Ying Yan, Jian Li, Xi Zhou, Xinying Xiang, Rong Huang, Xisheng Li, Changbei Ma, Xinmin Nie
Summary: As a representative biochemical indicator, alkaline phosphatase (ALP) plays a significant role in indicating and diagnosing clinical diseases. In this study, a signal-on fluorescence sensing method based on the enzyme-assisted target recycling (EATR) technique was developed for sensitive ALP activity detection. The method utilizes a two-step signal amplification process and demonstrates a sensitive limit of detection (LOD) and a linear range for quantifying ALP.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Pharmacology & Pharmacy
Weicong Ye, Longjie Li, Zishan Feng, Bocheng Tu, Zhe Hu, Xianjin Xiao, Tongbo Wu
Summary: A highly sensitive and selective method based on TdT and Endo IV was developed to quantify ALP activity. This method can detect the presence of ALP by detecting the elongation of a poly A tail, and has potential applications.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Electrochemistry
Qi Wang, Tingting Hao, Kaiyue Hu, Lingxia Qin, Xinxin Ren, Zhiyong Guo, Sui Wang, Yufang Hu
Summary: A novel circuit system with autonomous compensation solution ohmic drop is developed to improve the signal generation of traditional electrochemical biosensors. It is successfully applied for the analysis of terminal deoxynucleotide transferase (TdT) and its small molecule inhibitors, as well as mimicking TdT-related signal communication in various logic gates.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Multidisciplinary Sciences
Kristina Fredriksen, Stefanos Aivazidis, Karan Sharma, Kevin J. Burbidge, Caleb Pitcairn, Friederike Zunke, Eilrayna Gelyana, Joseph R. Mazzulli
Summary: GBA1 mutations that encode lysosomal beta-glucocerebrosidase (GCase) cause Gaucher disease and are risk factors for synucleinopathies. The study found that alpha-synuclein neuropathology induced by GCase depletion depends on neuronal maturity, alpha-synuclein physiology, and specific accumulation of long-chain glycosphingolipid substrates. Reduction of long-chain glycosphingolipids can ameliorate alpha-synuclein pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Physical
Xiaoyun Lu, Jinlong Li, Congyu Li, Qianqian Lou, Kai Peng, Bijun Cai, Ying Liu, Yonghong Yao, Lina Lu, Zhenyang Tian, Hongwu Ma, Wen Wang, Jian Cheng, Xiaoxian Guo, Huifeng Jiang, Yanhe Ma
Summary: By combining evolutionary analysis, mutagenesis scanning, and rational design, we have engineered ZaTdT, an enzyme that shows greatly improved catalytic activity for 3'-ONH2-modified nucleotides, enabling highly efficient enzymatic DNA synthesis.
Review
Biochemistry & Molecular Biology
Jon Ashley, Indiia G. Potts, Femi J. Olorunniji
Summary: The use of polymerase enzymes in biotechnology has provided unprecedented control over DNA manipulation, with terminal deoxynucleotidyl transferase (TdT) being one of the most intriguing enzymes. TdT is capable of template independent stepwise addition of nucleotides onto an oligonucleotide chain. This review comprehensively discusses the unique properties and applications of TdT as a biotechnology tool, and explores its increasing role in various fields.
Article
Chemistry, Analytical
Fangfang Wang, Yi Zhang, Desheng Chen, Zhuoqi Zhang, Zhengping Li
Summary: A sensitive Single Microbead (MB)-based Aptasensor coupled with Terminal deoxynucleotidyl transferase (TdT) triggered signal amplification (SMAT) was developed for detecting tumor-specific exosomal proteins. This method successfully detected exosomal EpCAM protein (Exo-EpCAM) from samples containing as low as 55 particles/µL tumor-derived exosomes, showing great potential for future clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Weicong Ye, Zhen Zhang, Caie Wang, Zishan Feng, Zhe Hu, Qingxu Liu, Tongbo Wu
Summary: Since the discovery of Terminal Protection of Small Molecule-Linked DNA (TPSMLD), TPSMLD-based assays have become widely used in studying small molecule-protein interactions and sensing. This study presents a simple, sensitive, and selective strategy for small molecule measurement using biotin as a model. By extending the terminal protection mechanism to the polymerase and incorporating a competition assay, a convenient turn-on and exponential signal amplification were achieved. This strategy demonstrated high selectivity and the ability to detect biotin with a low detection limit of 0.21 nM.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Xiaolong Zhang, Cheng Zheng, Lei Ding, Yanni Wu, Haipo Xu, Yupeng Sun, Yongyi Zeng, Xiaolong Liu, Jingfeng Liu
Summary: The study developed a dual amplified sensing strategy based on TdT and CRISPR-Cas12a for high selective and sensitive detection of PNK activity and inhibitor screening, enabling detection with linear range from 0 to 1 U/mL and a direct detection limit of 5 x 10(-4) U/mL.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Yudi Zheng, Dandan Hu, Di Wu, Kaiyue Hu, Xinxin Ren, Lingxia Qin, Zhiyong Guo, Sui Wang, Yufang Hu, Shaohua Ma
Summary: In this study, a novel and reversible electrochemical impedance strategy was developed for pH and TdT analysis. The strategy involves the generation of C-rich DNA by TdT, and monitoring the configuration change for pH analysis and TdT activity detection. This method shows promise for in situ monitoring of pH and TdT in complex clinical diagnosis.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Analytical
Qiong Liu, Kaiyue Hu, Yuqi She, Yufang Hu
Summary: A new electrochemical biosensor was designed to monitor and detect the release of H2O2 by rat astroglioma cells. The sensor exhibited excellent feasibility for physiological and pathological studies, and could also probe TdT activity and its inhibitors.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ying Yan, Han Zhao, Yukang Fang, Changbei Ma, Junxiang Chen
Summary: MiRNAs are important biomarkers in the biomedical industry, and detecting miRNA-21 levels accurately and early is a significant challenge. In this study, a dual amplification method based on strand displacement amplification and terminal deoxynucleotidyl transferase-assisted amplification was developed to detect miRNA-21. The method showed high sensitivity and specificity, and has great potential for clinical diagnosis.
Article
Pharmacology & Pharmacy
Tahoora Ghasemzadeh, Maliheh Hasannia, Khalil Abnous, Seyed Mohammad Taghdisi, Sirous Nekooei, Negar Nekooei, Mohammad Ramezani, Mona Alibolandi
Summary: The study designed and fabricated targeted nanobubbles with high encapsulation efficiency and sustained drug release. The decorated nanobubbles showed enhanced cellular uptake and cytotoxicity in MUC1-overexpressing cells. In vivo experiments demonstrated superior therapeutic efficacy and survival rate of the modified nanobubbles, and the echogenic capability was demonstrated through ultrasonic imaging.
EXPERT OPINION ON DRUG DELIVERY
(2023)
Article
Chemistry, Applied
Zahra Khoshbin, Marzieh Moeenfard, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: A simple aptasensor embedded in a micropipette tip is developed for label-free monitoring of acrylamide. The aptasensor utilizes the formation of a triple-helix molecular switch structure to induce the aggregation of gold nanoparticles. A smartphone imaging readout strategy enables the quantification of acrylamide based on the color change of the solution inside the micropipette tip. This aptasensor shows good selectivity and can successfully quantify acrylamide in various food samples.
Article
Nanoscience & Nanotechnology
Maliheh Hasannia, Kamran Lamei, Khalil Abnous, Seyed Mohammad Taghdisi, Sirous Nekooei, Negar Nekooei, Mohammad Ramezani, Mona Alibolandi
Summary: In this study, a novel PGA-PLA diblock copolymer was synthesized and used for the encapsulation of DOX and USPIONs to form Pep@USPIONs-DOX via a double emulsion method. AS1411 DNA aptamer was further decorated on the surface of PGA-PLA vesicles to form Apt-Pep@USPIONs-DOX. Targeted vesicles showed enhanced cellular uptake and cytotoxicity in 4T1 cell line compared to non-targeted vesicles.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Analytical
Zahra Khoshbin, Asma Verdian, Seyed Mohammad Taghdisi, Noor Mohammd Danesh, Khalil Abnous
Summary: In order to detect Pb2+ ions with high sensitivity and specificity, a simple optical biosensing array based on the cleavage activity of aptazyme strands and the regularity of liquid crystal molecules was introduced, which can detect ultra-low concentrations of Pb2+.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Sahraneshin Samani Somayeh, Seyed Mohammad Taghdisi, Sayed Ali Mortazavi, Tabatabaei Yazdi Farideh, Khalil Abnous
Summary: In this study, a novel U-shaped electrochemical biosensor was designed for the detection of Micrococcal nuclease (MNase). The biosensor utilized a gold electrode, thiol-modified oligonucleotide strands and their complementary strand (CS). The presence of MNase resulted in the digestion of the oligonucleotide sequences, allowing the redox agent to access the electrode surface and generate a current peak. The biosensor showed satisfactory results for MNase recognition in spiked milk samples.
Article
Chemistry, Medicinal
Elham Moradi, TaranehSadat Zavvar, Mona Alibolandi, Mohammad Ramezani, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: We evaluated DNA nanocarriers composed of multiple repeats of AS1411 and FOXM1 aptamers for targeted epirubicin delivery to breast cancer cells. The nanocarriers showed characteristics of being safe, stable, efficient epirubicin loading, pH-dependent drug release, and tumor-targeting ability in vitro and in vivo. The complex of the nanocarrier carrying epirubicin exhibited higher entry and cytotoxicity in target cells, higher therapeutic effect, and tumor drug accumulation compared to epirubicin alone.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2023)
Article
Biochemical Research Methods
Ghasem Anbiaee, Zahra Khoshbin, Javad Zamanian, Ali Samie, Mohammad Ramezani, Mona Alibolandi, Noor Mohammd Danesh, Seyed Mohammad Taghdisi, Khalil Abnous
Summary: To rapidly and easily determine the detrimental effects of drugs such as cocaine on the human body, the development of sensing methods is highly necessary. We designed a cost-effective fluorescent aptasensor for cocaine detection using the signal enhancement capability of the UiO-66/AuNPs nanocomposite. The aptasensor achieved a linear response range of 0.5 & mu;M-20 & mu;M with a low detection limit of 0.178 & mu;M. This method has substantial benefits for the development of a low-cost and facile tool in medicine and forensic science.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biochemical Research Methods
Zahra Khoshbin, Noor Mohammad Danesh, Morteza Alinezhad Nameghi, Mohammad Ramezani, Mona Alibolandi, Mersedeh Shayan, Ali Samie, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: In this study, a label-free fluorescent aptasensor was developed for monitoring trace levels of tobramycin. The aptasensor achieved a low detection limit of 150 pM and exhibited good selectivity towards tobramycin. It is a promising tool for clinical applications and food safety control.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biochemical Research Methods
Masoomeh Esmaelpourfarkhani, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: In this study, a label-free aptasensor utilizing colorimetric properties was developed for the detection of Pb2+ with high sensitivity. The approach involved the use of modified aptamer with enhanced oxidase-mimicking activity of MnO2 nanoflowers. This innovative method provides a simple and cost-effective means for monitoring Pb2+ ions without the need for labeling techniques.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biochemical Research Methods
Ghasem Anbiaee, Rozita Feizpour, Zahra Khoshbin, Mohammad Ramezani, Mona Alibolandi, Seyed Mohammad Taghdisi, Khalil Abnous
Summary: A novel label-free and enzyme-free fluorescence aptasensing assay using Sybr Green I (SGI) as the signal indicator was developed for the determination of kanamycin. The assay showed a considerable fluorescent signal due to the intercalation of SGI at the aptamer-complementary strand (Apt/CP) conjugate. The introduction of the target caused the separation of Apt from CP, leading to a decrease in the fluorescent signal, making it a sensitive and specific method for kanamycin detection.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Zahra Khoshbin, Seyed Mohammad Taghdisi, Noor Mohammad Danesh, Parirokh Lavaee, Ali Khakshour Abdolabadi, Khalil Abnous
Summary: A simple colorimetric strategy was developed for the monitoring of lead ion (Pb2+) using gold nanoparticles (AuNPs) regulated by aptamer strands. The aptamers masked the surface of AuNPs, preventing their peroxidase-like activity and the oxidation of TMB. The aptamer-Pb2+ complex, formed by adding Pb2+, released the aptamer from the AuNPs surface, enabling the oxidation of TMB and resulting in a color change from colorless to blue. The aptasensor showed a linear concentration range of 0.2 to 200 nM and a LOD of 0.056 nM for Pb2+ detection, successfully monitoring Pb2+ in drinking water, milk, and human serum, making it suitable for various applications.
ANALYTICAL LETTERS
(2023)
Article
Chemistry, Medicinal
Salimeh Mirzaei, Zahra Khademi, Reza Zolfaghari, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: In this study, a dual-targeting delivery system using mesoporous silica nanoparticles with hollow structures (HMSNs) was developed for specific delivery of epirubicin (EPI) to cancer cells and introducing a H+-triggered bubble generating nanosystem (BGNS). The results demonstrated that this system showed promising anti-cancer effects and reduced off-target effects.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2023)
Article
Pharmacology & Pharmacy
Fatemeh Alizadeh, Elnaz Yaghoobi, Maryam Imanimoghadam, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Using nanocarriers for targeted drug delivery can reduce side effects and improve therapeutic efficacy. This study developed nanoparticles that target mucin-1 and successfully demonstrated their effectiveness in targeting cancer cells.
JOURNAL OF DRUG TARGETING
(2023)
Article
Chemistry, Analytical
Masoomeh Esmaelpourfarkhani, Noor Mohammad Danesh, Mohammad Ramezani, Mona Alibolandi, Ali Khakshour Abdolabadi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: To combat drug trafficking and abuse, it is necessary to develop a sensing method for cocaine detection. This study proposes a label-free and simple split aptamer-based fluorescent aptasensor, which utilizes the N-methyl mesoporphyrin IX (NMM)/G-quadruplex system as a fluorescent indicator for cocaine detection. The working principle of the aptasensor is based on the formation of a split G-quadruplex structure in the presence of cocaine, leading to an increase in the emission intensity of fluorescence. Under optimized conditions, the designed fluorescent aptasensor exhibits a useful linear concentration range (50 pM to 25 nM) and a limit of detection (16 pM). The potential application of the designed aptasensor is demonstrated by successfully detecting cocaine in spiked human serum samples, with satisfying recoveries.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Monireh Bolourinezhad, Majid Rezayi, Zahra Meshkat, Saman Soleimanpour, Majid Mojarrad, Farkhonde Zibadi, Seyed Hamid Aghaee-Bakhtiari, Seyed Mohammad Taghdisi
Summary: This study developed a rapid and accurate biosensor for detecting SARS-CoV-2. It utilized MXene nanosheets and carbon platinum to amplify the signal upon hybridization reaction. The biosensor showed a limit of detection of 0.4 aM and demonstrated high accuracy and sensitivity in clinical samples.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
