Article
Biochemical Research Methods
Wei Wang, Sandra Thiemann, Qing Chen
Summary: Surface plasmon resonance (SPR) analysis is valuable for assessing binding characteristics of antibodies. This report presents an example of assay qualification for SPR analysis and recommends using K-D as the output for qualification.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Applied
Abhishek Mishra, Ekta Arjundas Kukreja, Ramesh Namdeo Pudake, Robin Kumar, Manoj Pratap Singh, Lalit Yadav, Ishan Pandey, Faheem A. Sheikh, Ravi Mani Tripathi
Summary: The overuse of antibiotics like ampicillin in developing and industrializing countries leads to the evolution of drug-resistant bacteria and adverse effects on health. To address this issue, a colorimetric assay using poly(vinyl alcohol)-capped silver nanoparticles was developed to detect ampicillin. The assay exhibited good selectivity and sensitivity, with a detection limit of 6.2 μM. This method provides a real-time and easy-to-use platform for detecting ampicillin in actual samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Hongtao Li, Yuting Zhang, Yan Huang, Dapeng Cao, Shitao Wang
Summary: A new facile turn-on fluorescence probe was developed for selectively detecting specific proteins, with controlled color change for protein detection. By utilizing dissolution-enhanced emission, aggregation-caused quenching was successfully suppressed, showing high potential applications in the field of protein detection.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Organic
Fengbo Liu, Shagor Chowdhury, Roselyne Rosas, Valerie Monnier, Laurence Charles, Hakim Karoui, Didier Gigmes, Olivier Ouari, Floris Chevallier, Christophe Bucher, Anthony Kermagoret, Simin Liu, David Bardelang
Summary: A viologen-phenylene-imidazole (VPI) conjugate can form antiparallel triple stacks in water with cucurbit[10]uril (CB[10]), pairwise complexing the guest trimer. The quinary host:guest 2:3 complex displays features assignable to charge-transfer interactions, and under reductive conditions, CB[10] can solubilize a VPI radical.
Article
Physics, Applied
Hao Xie, Minghuai Yu, Ruiqing Xing, Cheng Wang, Weixiang Ye
Summary: This study presents a versatile multiplex molecular affinity kinetics detection method based on single nanoparticle plasmon sensors using frequency-domain optical measurement. With the single-color imaging NanoSPR method, high-throughput biosensing potential is achieved, and results of equilibrium dissociation constants are in agreement with traditional techniques.
APPLIED PHYSICS LETTERS
(2022)
Article
Chemistry, Multidisciplinary
David Milicevic, Jan Hlavac
Summary: A new, robust and reliable method for screening three proteases in a single-enzyme mode has been developed using a multi-purpose peptide probe and multiple fluorophores. The method allows for the detection of trypsin, chymotrypsin and caspase-8 at very low concentrations. Additionally, the simultaneous screening of two proteases using the developed probe was found to be only partially feasible, mainly due to unselective chymotrypsin cleavage.
Article
Chemistry, Multidisciplinary
Tianqing Zhang, Bryan Wei
Summary: A rational design scheme for building synthetic nucleic acid allosteric nanodevices was presented, inspired by allosteric regulation of natural molecules. The scheme includes developing DNA switches with triplex-forming oligos as allosteric modulators, implementing designated allosteric transitions, and applying toehold-mediated strand displacement for synthetic nucleic acid computing.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Oncology
Xun-Xi Lu, Wen-Xiao Yang, Yu -Chen Pei, Hong Luo, Xiao-Guang Li, Yun-Jin Wang, Guo-Liang Zhang, Hong Ling, Zhi-Ming Shao, Xin Hu
Summary: Dysregulation of RNA-binding proteins (RBPs) is a characteristic of cancer. Investigating the functions and mechanisms of abnormal RBPs can help identify new cancer biomarkers and treatment strategies. In this study, SNRPC was identified as an oncogenic modulator in triple-negative breast cancer (TNBC). SNRPC upregulation was associated with poor prognosis, and its inhibition impaired TNBC cell proliferation, migration, and invasion. SNRPC also contributed to the stability of U1 snRNP and the transcriptional program controlled by RNA Pol II. Knockdown of SNRPC reduced the expression of oncogenes and inhibited TNBC progression via the TNFAIP2-Rac1-β-catenin signaling pathway.
Article
Pharmacology & Pharmacy
Anna Vlachodimou, Henk de Vries, Milena Pasoli, Miranda Goudswaard, Soon-Ai Kim, Yong-Chul Kim, Mirko Scortichini, Melissa Marshall, Joel Linden, Laura H. Heitman, Kenneth A. Jacobson, Adriaan P. IJzerman
Summary: A(2B) adenosine receptor (A(2B)AR) antagonists have therapeutic potential in inflammation-related diseases, but there is a need for novel antagonists. This study investigates the synthesis and biological evaluation of xanthine derivatives as A(2B)AR antagonists. The study finds a clear relationship between a ligand's A(2B)AR residence time and its functional effect, highlighting the importance of binding kinetics in early drug discovery.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Acoustics
Le Zhang, Lixian Liu, Xueshi Zhang, Xukun Yin, Huiting Huan, Huanyu Liu, Xiaoming Zhao, Yufei Ma, Xiaopeng Shao
Summary: Enhancing multi-gas detectability is crucial for dissolved gas sensing application, and this study presents a photoacoustic spectroscopy technique capable of simultaneous detection, high selectivity, and less cross-interference. A T-type photoacoustic cell is designed and verified to be an appropriate sensor, with resonance frequencies determined by absorption and resonant cylinders. Multi-gas detection capability is demonstrated by measuring CO, CH4, and C2H2 simultaneously using different excitation sources, and the influence of cross-sensitivity towards humidity is examined. The minimum detection limits for CO, CH4, and C2H2 are experimentally determined, showing the effectiveness of the technique.
Article
Agriculture, Dairy & Animal Science
G. Niero, G. Meoni, L. Tenori, C. Luchinat, G. Visentin, S. Callegaro, E. Visentin, M. Cassandro, M. De Marchi, M. Penasa
Summary: This study investigated the use of proton nuclear magnetic resonance spectroscopy to discriminate between milk produced before and after the grazing season, and to assess the effects of grazing on milk metabolites. The results showed that the H-1 NMR spectra of milk were promising for identifying milk produced under different conditions. Additionally, grazing was found to significantly impact the metabolic profile of milk, suggesting the potential use of several metabolites as indicators of farm management.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Spectroscopy
Xiao Cheng, Chen Chen Wang, Anran Liu, Yu Liu, Jingjiao Dong, Zhi Wang, Wei Wei, Songqin Liu
Summary: A fluorescence immunoassay for the simultaneous detection of three Gram-positive foodborne pathogenic bacteria was proposed. The method used a polycarbonate chip immobilized with Van-BSA to capture the target bacteria and CdSe/ZnS quantum dot modified antibodies for detection. The assay showed good performance with low detection limits and accuracy.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Carson C. Cole, Le Tracy Yu, Mikita Misiura, Joseph Williams III, Thi H. Bui, Jeffrey D. Hartgerink
Summary: Collagen mimetic peptides are made up of triple helices, and their design and synthesis are challenging. In this study, researchers compared the effects of different charge pairs on the triple helical structure and applied their findings to the design and synthesis of collagen.
Article
Chemistry, Analytical
Chunyan Wang, Cuiyan Han, Xiaoxue Du, Weiwei Guo
Summary: A versatile CRISPR-Cas12a-based biosensing platform was developed in this study by introducing an entropy-driven dynamic DNA network, allowing detection of various types of targets with low detection limits for nucleic acid and protein analytes. The platform also demonstrated good applicability in human serum samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Instruments & Instrumentation
Karishma Khatoon, Asgar Ali, Fahan J. Ahmad, Zubair Hafeez, M. Moshahid A. Rizvi, Sohail Akhter, Sarwar Beg
Summary: The research focused on designing and optimizing a nanoemulsion gel formulation for concurrent delivery of curcumin, resveratrol, and thymoquinone, showing improved anti-psoriatic activity with good texture profile and anti-angiogenic properties.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Article
Chemistry, Analytical
Kishan Rijal, Raj Mutharasan
Article
Chemistry, Analytical
Blake N. Johnson, Raj Mutharasan
ANALYTICAL CHEMISTRY
(2012)
Article
Chemistry, Analytical
Harsh Sharma, Raj Mutharasan
ANALYTICAL CHEMISTRY
(2013)
Article
Chemistry, Analytical
Blake N. Johnson, Harsh Sharma, Raj Mutharasan
ANALYTICAL CHEMISTRY
(2013)
Article
Chemistry, Analytical
Harsh Sharma, Raj Mutharasan
ANALYTICAL CHEMISTRY
(2013)
Review
Biophysics
Blake N. Johnson, Raj Mutharasan
BIOSENSORS & BIOELECTRONICS
(2012)
Article
Physics, Applied
Blake N. Johnson, Raj Mutharasan
JOURNAL OF APPLIED PHYSICS
(2011)
Article
Engineering, Electrical & Electronic
Blake N. Johnson, Raj Mutharasan
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2011)
Article
Chemistry, Physical
Blake N. Johnson, Raj Mutharasan
JOURNAL OF PHYSICAL CHEMISTRY C
(2013)
Article
Chemistry, Multidisciplinary
Blake N. Johnson, Raj Mutharasan
Article
Chemistry, Multidisciplinary
Kaylin M. McMahon, R. Kannan Mutharasan, Sushant Tripathy, Dorina Veliceasa, Mariana Bobeica, Dale K. Shumaker, Andrea J. Luthi, Brian T. Helfand, Hossein Ardehali, Chad A. Mirkin, Olga Volpert, C. Shad Thaxton
Article
Multidisciplinary Sciences
Yoshihiko Ichikawa, Marina Bayeva, Mohsen Ghanefar, Vishnu Potini, Lin Sun, R. Kannan Mutharasan, Rongxue Wu, Arineh Khechaduri, Tejaswitha Jairaj Naik, Hossein Ardehali
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Chemistry, Analytical
Harsh Sharma, Raj Mutharasan
SENSORS AND ACTUATORS B-CHEMICAL
(2012)
Meeting Abstract
Cardiac & Cardiovascular Systems
Yoshihiko Ichikawa, Marina Bayeva, Mohsen Ghanefar, R. Kannan Mutharasan, Rongxue Wu, Hossein Ardehali
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)