Article
Microbiology
Antti J. Rissanen, Tom Jilbert, Asko Simojoki, Rahul Mangayil, Sanni L. Aalto, Ramita Khanongnuch, Sari Peura, Helena Jantti
Summary: Eutrophication increases methane emissions from lake sediments by affecting the structure of methane-producing and consuming microbial communities.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Antti J. Rissanen, Tom Jilbert, Asko Simojoki, Rahul Mangayil, Sanni L. Aalto, Ramita Khanongnuch, Sari Peura, Helena Jantti
Summary: Eutrophication increases the input of labile organic matter into lake sediments, which affects the microbial community structure involved in methane production and consumption.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agricultural Engineering
Jing Zhao, Yu Li, Zhenhua Zhang
Summary: This study investigates the impact of operational parameters (HRT and pressure) on the microbiome of high-pressure anaerobic digestion (HPAD). The results reveal that low HRT leads to volatile fatty acids accumulation and pressure significantly affects the bacterial community. Furthermore, in continuous HPAD operation, optimizing HRT is crucial for CH4 production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Jing Yu, Jie Gu, Xiaojuan Wang, Liusheng Lei, Honghong Guo, Zilin Song, Wei Sun
Summary: Inoculation with lignocellulose-degrading microorganisms (LDM) was found to accelerate the degradation of organic matter and increase methane emissions during composting. LDM also significantly increased the abundances of bacterial and methanogenic community during the later thermophilic period. The study suggests that organic matter and temperature are the main factors affecting the bacterial and methanogen community structures.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Engineering, Environmental
Haitao Wang, Gerald Jurasinski, Jana Taeumer, Andreas W. Kuss, Verena Gross, Daniel Koehn, Anke Guenther, Tim Urich
Summary: Using a quantitative metatranscriptomic approach, this study investigated the changes in the transcript abundances of methanogen and methanotroph rRNA, as well as mcrA and pmoA mRNA, during a summer drought in rewetted peatlands in northern Germany. The results showed that drought altered the community structure of methane-cycling microbiomes, decreased CH4 fluxes and transcript abundances of methanogens and methanotrophs, and there was no recovery or increase after the drought ended. Importantly, the study revealed that the relationship between methanogens and methanotrophs is the key determinant of CH4 turnover.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Microbiology
Qiuyun Jiang, Hongmei Jing, Hao Liu, Mengran Du
Summary: This study investigated microbial communities in cold seep and trough sediments in the South China Sea and observed differences in biogeography and gene abundance. The study found closely related genotypes in wetland and mangrove microbial communities and higher relative abundance of the NC10 group in the Xisha trough. The study highlights the importance of combining functional genes and 16S rRNA genes to understand population shifts in natural microbial communities among different oceanic regions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
R. G. Bulgarelli, M. F. A. Leite, M. De Hollander, P. Mazzafera, S. A. L. Andrade, E. E. Kuramae
Summary: The availability of soil phosphorus can affect plant growth, root-soil interactions, and rhizosphere microbial community composition. This study investigated the rhizosphere microbial communities of 24 species of eucalypts under low or sufficient soil phosphorus availability. It was found that eucalypt species identity was the main factor determining the composition of the rhizosphere microbial community, while the effect of phosphorus availability varied among species.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Ecology
Robert Starke, Victor Satler Pylro, Daniel Kumazawa Morais
Summary: Sequencing 16S rRNA gene amplicons is commonly used to study prokaryotic communities, but gene copy normalization (GCN) does not improve the accuracy of results. The study questions the use of GCN for metataxonomic surveys until a more comprehensive catalogue of copy numbers becomes available.
Article
Microbiology
Kayla M. Williamson, Brandie D. Wagner, Charles E. Robertson, Mark J. Stevens, Marci K. Sontag, Peter M. Mourani, J. Kirk Harris
Summary: The study aimed to increase the sensitivity of bacterial community analysis without impacting community composition or interfering with cluster formation during sequencing. The modified PCR protocol showed increased sensitivity in detecting bacterial communities in airway samples, crucial for studying the highly variable human airway microbiome.
Article
Environmental Sciences
Yo-Jin Shiau, Yun-An Chen, Chong-Ren You, Yi-Chieh Lai, Mengshan Lee
Summary: Constructed wetlands in tropical regions show higher soil organic carbon content in older wetlands, while recalcitrant carbon content remains stable. This suggests that bioavailable carbon sources for microbial growth increase with wetland age.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Kyohei Kuroda, Futaba Shinshima, Shoichi Tokunaga, Taro Q. P. Noguchi, Masahito Yamauchi, Masaru K. Nobu, Takashi Narihiro, Masayoshi Yamada
Summary: The use of green tuff (GT) has been shown to enhance methane-producing activity (MPA) and improve the microbial community composition, thus promoting methane production. Community profiling analysis revealed higher populations of syntrophic bacteria and hydrogenotrophic methanogens in cultures with GT, indicating its potential for activating syntrophic partnerships and enhancing reactor performance.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Energy & Fuels
Saowaluck Haosagul, Sukunya Oaew, Peerada Prommeenate, Vanatpornratt Sawasdee, Nipon Pisutpaisal
Summary: This study designed a microarray based on the entire genome and functional genes for monitoring the sulfur oxidizing bacteria in the starch industry, demonstrating high-quality DNA concentration and yield. The expression level of recirculating sludge increased compared to starting sludge, with some bacteria gaining higher gene expression levels related to sulfide oxidation.
Article
Agricultural Engineering
Parisa Ghofrani-Isfahani, Panagiotis Tsapekos, Maria Peprah, Panagiotis Kougias, Xinyu Zhu, Adam Kovalovszki, Athanasios Zervas, Xiao Zha, Carsten S. Jacobsen, Irini Angelidaki
Summary: The study evaluated four different types of ceramic gas distributors to increase biomethane formation during ex-situ biogas upgrading process. SiC gas distributors effectively improved biomethane content fulfilling natural gas standards, with microbial analysis showing the importance of H-2 in enhancing hydrogenotrophic methanogenesis. Adjusting the anaerobic bioconversion model was effective for simulating the operation of up-flow reactors in biogas upgrading process.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
Konstantina Tsigkou, Antonia Terpou, Laura Treu, Panagiotis G. Kougias, Michael Kornaros
Summary: The study focused on utilizing olive mill wastewater for efficient biogas production under thermophilic conditions, testing its performance and analyzing the microbial community. Results showed potential for further exploitation of olive mill wastewater for biogas production.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Parasitology
Sandor Hornok, Andrei D. Mihalca, Jeno Kontschan, Nora Takacs, Denis Fedorov, Olivier Plantard, Attila D. Sandor
Summary: This study provides high-resolution digital pictures and molecular data of male and female I. rugicollis for the first time, and morphological comparison with I. cornutus could not resolve uncertainties in the latter species' validity. Further molecular comparisons with new specimens of I. cornutus are needed to confirm its status. This study represents the first comprehensive molecular phylogenetic analysis of western Palearctic Pholeoixodes species, including I. rugicollis, and confirms the phylogenetic position of the subgenus Eschatocephalus within Pholeoixodes.
PARASITES & VECTORS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Roland Wirth, Gergely Maroti, Lidia Liptak, Monika Mester, Alaa Al Ayoubi, Bernadett Pap, Melinda Madlena, Janos Minarovits, Kornel L. Kovacs
Summary: Important alterations were found in the microbiomes of supragingival biofilm and saliva samples from adolescent patients developing induced or spontaneous gingivitis compared to healthy controls. Certain taxa showed characteristic features of potential diagnostic value, highlighting the need for rigorous bioinformatics analysis to avoid misinterpretations.
Article
Entomology
Liliana Toth, Peter Poor, Attila ordog, Gyorgyi Varadi, Attila Farkas, Csaba Papp, Gabor Bende, Gabor K. Toth, Gabor Rakhely, Florentine Marx, Laszlo Galgoczy
Summary: Plant pathogenic fungi cause significant losses in crop production worldwide. Developing new strategies for plant and crop protection is crucial due to the resistance of these fungi to approved chemical fungicides. A study investigated the potential of using extracellular antifungal proteins (AFPs) and their peptide derivatives (PDs) as combinatorial biofungicides for plant and crop protection. The study found that the combination of AFPs and PDs can effectively inhibit the growth of various fungi and mitigate the symptoms of fungal infection on tomato plants.
Article
Pharmacology & Pharmacy
Sandor Vaczi, Lilla Barna, Krisztian Laczi, Ferenc Tomosi, Gabor Rakhely, Botond Penke, Livia Fulop, Ferenc Bogar, Tamas Janaky, Maria A. Deli, Zsofia Mezei
Summary: This study examined the effects of sigma-1 receptor ligands on platelet and vascular eicosanoid synthesis. The results showed that these ligands can modify the synthesis of eicosanoids in platelets and vessels and play a role in multiple steps of arachidonic acid metabolism.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nikolett Miklovics, Agnes Duzs, Fanni Balogh, Gabor Paragi, Gabor Rakhely, Andras Toth
Summary: This study reports the characteristics of the quinone binding site (QBS) of a type VI sulfide:quinone oxidoreductases (SQR) from the photosynthetic purple sulfur bacterium Thiocapsa roseopersicina. The study identifies the amino acids near the enzyme-bound quinone ring and their interaction with the quinone head group. Mutant experiments further confirm the importance of these interactions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Etelka Kovacs, Csilla Szucs, Attila Farkas, Mark Szuhaj, Gergely Maroti, Zoltan Bagi, Gabor Rakhely, Kornel L. Kovacs
Summary: This study investigated the decomposition of lignocellulosic plant biomass by four filamentous fungi. It was found that the Penicillium aurantiogriseum strain exhibited excellent hydrolytic enzyme production and generated the highest biogas yield from partially degraded substrates. Corn stover was the most suitable substrate for biomass decomposition and biogas production.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Virology
Norbert Acs, Ross Holohan, Laura J. J. Dunne, Adrian R. R. Fernandes, Adam G. G. Clooney, Lorraine A. A. Draper, R. Paul Ross, Colin Hill
Summary: The human microbiome plays a critical role in health and disease. Disruptions in the gut microbiome composition, including bacteria and bacteriophages, are associated with intestinal diseases. In vitro fermentation systems can provide insights into the short-term impact of bacteriophage transplants on the bacterial and viral composition in human recipients.
Article
Biotechnology & Applied Microbiology
Gergely Erno Lakatos, Karolina Ranglova, Daniela Barcenas-Perez, Tomas Grivalsky, Joao Camara Manoel, Mykola Mylenko, Jose Cheel, Jozsef Nyari, Roland Wirth, Kornel L. Kovacs, Jiri Kopecky, Linda Nedbalova, Jiri Masojidek
Summary: Three cultivation regimes were tested for cold-adapted cultures of Monoraphidium in an outdoor pond: sunlight cultivation, combination of sunlight and supplementary illumination, and nitrogen depletion using both light sources. The highest productivity was achieved when sunlight was combined with supplementary illumination, with a specific growth rate of 0.191 d-1. Nitrogen depletion stress resulted in the highest total fatty acid content of 20.29% DW, with oleic acid accounting for 54% of the total fatty acid content. The cultivation under sunlight in the first trial yielded a significant amount of lutein, 26.39 mg lutein g-1 DW. In the second trial, 236 mLN g-1oTS of methane was generated from the harvested and fermented biomass.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Ecology
Roland Wirth, Zoltan Bagi, Prateek Shetty, Mark Szuhaj, Teur Teur Sally Cheung, Kornel L. Kovacs, Gergely Maroti
Summary: Multi-omics analysis was used to study inter-kingdom interactions in biogas-producing microbial communities. The microbiomes of three biogas digesters were analysed using a metagenomics framework, revealing the relationship between methanogenic communities and their bacterial partners. The study identified high-quality metagenome-assembled genomes and found that the abundance of microorganisms was independent of their activity. The most active metabolic pathway was found to be methanogenesis.
Article
Biochemistry & Molecular Biology
Alajos Berczi, Zsuzsanna Marton, Krisztina Laskay, Andras Toth, Gabor Rakhely, Agnes Duzs, Krisztina Sebok-Nagy, Tibor Pali, Laszlo Zimanyi
Summary: Cytochrome b561 proteins are integral membrane proteins that have ascorbate reducibility and trans-membrane electron transferring capability. They have been found in various animal and plant phyla and are localized in different membranes. Two homologous proteins in humans and rodents are thought to be involved in cancer pathology. This paper presents the physical-chemical properties of the homologues in mice and humans, providing insights into the CYB561 protein family.
Article
Biochemistry & Molecular Biology
Gyorgyi Varadi, Zoltan Kele, Andras Czajlik, Attila Borics, Gabor Bende, Csaba Papp, Gabor Rakhely, Gabor K. Toth, Gyula Batta, Laszlo Galgoczy
Summary: Due to the fast spread of drug resistance, only a limited number of drugs can be used to treat fungal infections. Therefore, there is an urgent need to develop new antifungal treatment strategies. The NFAP2 protein secreted by the mold Neosartorya shows promise as a template for future antifungal drug design, but it is difficult to determine its disulfide linkage pattern using traditional mass spectrometry-based methods.
Article
Pharmacology & Pharmacy
Balint Racz, Annamaria Kincses, Krisztian Laczi, Gabor Rakhely, Enrique Dominguez-alvarez, Gabriella Spengler
Summary: Recently, the anticancer activity of symmetrical selenoesters was investigated in sensitive and resistant human colon adenocarcinoma cells. The compounds showed synergistic interaction with doxorubicin and exhibited ABCB1 inhibitory and apoptosis-inducing effects. Transcriptome analysis revealed that symmetrical selenoesters may influence the redox state of cells and interfere with metastasis formation.
Article
Biotechnology & Applied Microbiology
Mark Szuhaj, Balazs Kakuk, Roland Wirth, Gabor Rakhely, Kornel Lajos Kovacs, Zoltan Bagi
Summary: This study investigated the biomethane formation from 4 H-2 + CO2 by two methanogens, Methanocaldococcus fervens and Methanobacterium thermophilum. The research aimed to understand the regulation of biomethane biosynthesis by H-2. It was found that the autotrophic methanogen M. fervens could be easily controlled by H-2/CO2, while the heterotrophic methanogen M. thermophilum was less sensitive to H-2/CO2, suggesting the importance of the biomethane-generating methanogenic community in renewable excess electricity conversion and storage systems.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Noemi N. Horvath-Gonczi, Zoltan Bagi, Mark Szuhaj, Gabor Rakhely, Kornel L. Kovacs
Summary: Bioelectrochemical systems (BESs) have great potential in renewable energy production technologies, with the ability to generate electricity via Microbial Fuel Cell (MFC) or synthesize valuable commodities through Microbial Electrolysis Cells (MECs). Although industrial-scale operation still faces difficulties, various reactor configurations and operational protocols are rapidly increasing. This article reviews recent BES literature, focusing on electrosynthesis and promising reactor configurations, while attempting to clarify the numerous definitions proposed for BESs. The main components of BESs are highlighted, and useful and generally applicable operational parameters are collected for comparative studies.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Buta Singh, Kornel L. Kovacs, Zoltan Bagi, Mate Petrik, Gabor L. Szepesi, Zoltan Simenfalvi, Zoltan Szamosi
Summary: This study investigated the optimum mixing regime in a lab-scale digester by continuously monitoring the physicochemical properties of the digestate. The results showed a reduction in biogas yield as the time between mixing operations increased. The highest biogas yield was observed at a mixing frequency of 5 min/h.
FERMENTATION-BASEL
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)