Article
Agricultural Engineering
Pseudomonas Asiatica, Trinh Thi Nguyen, Suman Lama, Satish Kumar Ainala, Mugesh Sankaranarayanan, Ashish Singh Chauhan, Jung Rae Kim, Sunghoon Park
Summary: The study demonstrates that Pseudomonas asiatica C1 can be a promising host for the production of 3-HP from glycerol by genetic editing and optimization, achieving high production efficiency and molar yield on glucose.
BIORESOURCE TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Xiao-Ran Jiang, Xu Yan, Lin-Ping Yu, Xin-Yi Liu, Guo-Qiang Chen
Summary: The study describes the engineering of Halomonas bluephagenesis to achieve high-level production of 3-hydroxypropionic acid (3HP) and its copolymer under open and unsterile conditions by deleting a newly identified degradation pathway and balancing redox state.
NATURE COMMUNICATIONS
(2021)
Article
Polymer Science
Sai Nandhini Ravi, Mugesh Sankaranarayanan
Summary: This study aims to improve the co-production of propanediol (PDO) and 3-hydroxypropionic acid (3-HP) by isolating a new strain of K. quasipneumoniae MSN12 (KpMSN12) and overexpressing the aldH and dhaB genes. The results showed that the combined overexpression of aldH and dhaB in KpMSN12 led to higher 3-HP and PDO production, as well as improved sedimentation characteristics and increased susceptibility to antibiotics.
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
(2023)
Article
Agriculture, Multidisciplinary
Ya'nan Shi, Rongshan Li, Jie Zheng, Yubin Xue, Yong Tao, Bo Yu
Summary: This study explores a new route for propionate production using the robust oxidization capacity of Pseudomonas putida KT2440 strain. By optimizing gene expression and eliminating intermediate products, high-purity propionate is produced from 1,2-propanediol with high efficiency.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ayaat M. Mahmoud, Cameron J. Nowell, Orlagh Feeney, Leonie van 't Hag, Thomas P. Davis, Kristian Kempe
Summary: An in-depth understanding of the physicochemical properties of nanocarriers is crucial for the development of novel delivery systems. In this study, well-defined hydrophobic carboxylated poly(3-hydroxypropionate)-based comb polymers were synthesized and characterized. The study demonstrated the importance of hydrophobicity in tuning the cellular interactions of carboxylated polymers for future drug carrier design.
Article
Microbiology
Yidong Liu, Wenxuan Sun, Liting Ma, Rong Xu, Chunyu Yang, Ping Xu, Cuiqing Ma, Chao Gao
Summary: Given the intrinsic and potential resistance of Pseudomonas aeruginosa to antibiotics, it is crucial to develop new therapeutic options for infections caused by this bacterium. Bacterial metabolic pathways are being explored as potential targets for new antibiotics. This study investigates the metabolic mechanisms of glycerol and its intermediate glycerol 3-phosphate (G3P) in P. aeruginosa PAO1. The results reveal the importance of G3P homeostasis for the growth and virulence factor production of P. aeruginosa PAO1. The enzyme GlpD, involved in G3P catabolism, is identified as a potential therapeutic target for the prevention and treatment of P. aeruginosa infections.
Article
Biochemistry & Molecular Biology
Mohammad Mojarrad, Takahisa Tajima, Akiko Hida, Junichi Kato
Summary: This study evaluated the simultaneous biosynthesis of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) using a new psychrophile-based simple biocatalyst (PSCat) reaction system. The maximum production of 3-HP was 62.0 mm in solo production and 25.0 mm for 1,3-PDO. Through the PSCat approach, productivity was boosted with a 13.5 mm increase in 3-HP and 4.9 mm increase in 1,3-PDO in the simultaneous production process.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Li Liu, Daniel M. Schubert, Martin Konneke, Ivan A. Berg
Summary: Ammonia-oxidizing archaea of the phylum Thaumarchaeota play a crucial role in oceanic and soil nitrification, as well as dark ocean primary production. They utilize an energetically efficient version of the 3-hydroxypropionate/4-hydroxybutyrate cycle to assimilate inorganic carbon. The protein Nmar_1028 identified in this study is a key enzyme in this cycle and essential for the functioning of the important marine archaeon Nitrosopumilus maritimus.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Albert Gyapong Aduhene, Hongliang Cui, Hongyi Yang, Chengwei Liu, Guangchao Sui, Changli Liu
Summary: Poly(3HP) is a novel biomaterial with biocompatibility, biodegradability, and high yield synthesis, which has been widely used in the bioplastic industry. The development of bio-polymer-based functional composites helps increase the value of raw microbial-biopolymers obtained from cheap sources.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Junhua Yun, Hossain M. Zabed, Yufei Zhang, Amreesh Parvez, Guoyan Zhang, Xianghui Qi
Summary: The study successfully developed a co-culture method for producing 1,3-propanediol without the need for vitamin B-12, resulting in a higher yield of 1,3-PD. The co-fermentation in a 10 L bioreactor achieved a high productivity of 41.65 g/L 1,3-PD, showcasing the potential for cost-effective and efficient bioproduction.
BIORESOURCE TECHNOLOGY
(2021)
Article
Materials Science, Characterization & Testing
Mayte M. Quispe, Olivia Lopez, Dhahabia Abdallah Boina, Jean-Francois Stumbe, Marcelo A. Villar
Summary: Different additives studied had significant effects on the microstructure and properties of PHB films, with GTA and GTB showing little impact with the best mechanical performance, while G and PGs decreased the mechanical properties of the films.
Article
Biotechnology & Applied Microbiology
Ye Zhang, Qing Sun, Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: This study developed an antibiotic-free plasmid stabilization system for V. natriegens, enabling the engineered strain to stably maintain a large expression plasmid and increase the productivity of 1,3-propanediol and 3-hydroxypropionate.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Biotechnology & Applied Microbiology
Lin Shu, Qinghui Wang, Weiyan Jiang, Marina Tisma, Beakrock Oh, Jiping Shi, Gary J. Lye, Frank Baganz, Dong Wei, Jian Hao
Summary: This study revealed that glycerol catabolism and 1,2-propanediol catabolism in K. pneumoniae are undertaken by glycerol dehydratase and diol dehydratase, respectively. The Pdu microcompartment reduces the accumulation of 3-hydroxypropionaldehyde in the fermentation broth. PduG can cross reactivate glycerol dehydratase, while DhaF is not involved in the reactivation of diol dehydratase.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Agricultural Engineering
Doan-Thanh Ta, Chung-Jen Chiang, Zhu-Xuan Huang, Nguyen-Luan Luu, Yun-Peng Chao
Summary: By introducing the synthesis pathway of poly(3-hydroxybutyrate) (PHB) into a strain of Escherichia coli capable of efficiently utilizing glycerol, and reprogramming the central metabolism, the production of PHB was significantly improved. Through fed-batch fermentation, the PHB titer, content, and productivity reached 36.3±3.0 g/L, 66.5±2.8%, and 1.2±0.1 g/L/h, respectively. The technology platform developed shows great promise for the production of bio-plastics.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kevin Wang, Jonathan Hirschenson, Amanda Moore, Ryan J. Mailloux
Summary: This study found that the glutathionylation of mitochondrial complex I can lead to increased production of ROS during reverse electron transfer from the ubiquinone pool. This has implications for understanding the relationship between mitochondrial glutathionylation reactions and oxidative stress induction in various pathologies.
Article
Biotechnology & Applied Microbiology
Bo Liu, Shuman Xiang, Guang Zhao, Bojun Wang, Yanhe Ma, Weifeng Liu, Yong Tao
METABOLIC ENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Xinjun Feng, Wenjie Gao, Yifei Zhou, Zhiqiang Zhao, Xiutao Liu, Xiaojuan Han, Mo Xian, Guang Zhao
ENGINEERING IN LIFE SCIENCES
(2019)
Article
Biotechnology & Applied Microbiology
Huanhuan Liu, Jing Zhang, Jian Yuan, Xiaolong Jiang, Lingyan Jiang, Guang Zhao, Di Huang, Bin Liu
BIOTECHNOLOGY FOR BIOFUELS
(2019)
Review
Biotechnology & Applied Microbiology
Zhe Zhao, Mo Xian, Min Liu, Guang Zhao
BIOTECHNOLOGY FOR BIOFUELS
(2020)
Article
Biochemical Research Methods
Min Liu, Yingxin Fu, Wenjie Gao, Mo Xian, Guang Zhao
ACS SYNTHETIC BIOLOGY
(2020)
Article
Biochemical Research Methods
Jia Zhang, Yefei Wang, Baihui Chai, Jichao Wang, Lulu Li, Min Liu, Guang Zhao, Lishan Yao, Xiaolian Gao, Yifeng Yin, Jian Xu
ACS SYNTHETIC BIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Yifei Zhou, Yamei Ding, Wenjie Gao, Jichao Wang, Xiutao Liu, Mo Xian, Xinjun Feng, Guang Zhao
BIOTECHNOLOGY FOR BIOFUELS
(2020)
Article
Multidisciplinary Sciences
Ying Xu, Zhe Zhao, Wenhua Tong, Yamei Ding, Bin Liu, Yixin Shi, Jichao Wang, Shenmei Sun, Min Liu, Yuhui Wang, Qingsheng Qi, Mo Xian, Guang Zhao
NATURE COMMUNICATIONS
(2020)
Article
Biotechnology & Applied Microbiology
Shenmei Sun, Yamei Ding, Min Liu, Mo Xian, Guang Zhao
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jichao Wang, Xinyue Sui, Yamei Ding, Yingxin Fu, Xinjun Feng, Min Liu, Youming Zhang, Mo Xian, Guang Zhao
Summary: The study presents a genome-editing method based on a Rock-Paper-Scissors strategy, allowing for efficient genetic modifications with reduced cultivation times. The method has been successfully applied in Escherichia coli and Klebsiella pneumoniae, demonstrating its ability to prevent spontaneous genome mutations.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Min Liu, Likun Guo, Yingxin Fu, Meitong Huo, Qingsheng Qi, Guang Zhao
Summary: Protein acetylation has potential in metabolic regulation, but the regulatory mechanisms are not fully understood.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biotechnology & Applied Microbiology
Min Liu, Meitong Huo, Changshui Liu, Likun Guo, Yamei Ding, Qingjun Ma, Qingsheng Qi, Mo Xian, Guang Zhao
Summary: Protein lysine acetylation plays an important role in metabolic regulation, and can be used to control lactate synthesis, improve production and conversion efficiency in microbial synthesis, and provide insights into the treatment of lactate-related diseases.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Zhe Zhao, Ying Xu, Bo Jiang, Qingsheng Qi, Ya-Jie Tang, Mo Xian, Jichao Wang, Guang Zhao
Summary: The CpxRA system senses environmental stresses and regulates gene transcription for adaptation. This study identified over 10,000 putative CpxR binding sites and confirmed experimentally that 73 candidate genes are regulated by CpxR. These findings greatly expand our understanding of the CpxRA-dependent stress response network in Escherichia coli.
Article
Multidisciplinary Sciences
Likun Guo, Min Liu, Yujia Bi, Qingsheng Qi, Mo Xian, Guang Zhao
Summary: In this study, a synthetic carbon conserving pathway named SCTPK was constructed and optimized to improve the carbon atom economy in microbial cell factories. The pathway utilizes a generalist phosphoketolase Xfspk to convert glucose into stoichiometric amounts of acetylphosphate (AcP), and genetic circuits responding to AcP were created. Together with SCTPK, they form a gene-metabolic oscillator that autonomously regulates the production of valuable chemicals in response to intracellular AcP level.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Xiutao Liu, Xinjun Feng, Yamei Ding, Wenjie Gao, Mo Xian, Jichao Wang, Guang Zhao
METABOLIC ENGINEERING
(2020)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
