Article
Materials Science, Paper & Wood
Marlon Gualberto Ganter de Moura, Thiago Alessandre da Silva, Arion Zandona Filho, Marcos Lucio Corazza, Luiz Pereira Ramos
Summary: In this study, statistical design and mathematical modeling were used to investigate the fed-batch enzymatic hydrolysis process of steam-exploded sugarcane bagasse. A Box-Behnken experimental design was used to evaluate the effects of enzyme loading, stirring speed, and substrate total solids on glucose release after 48 hours of hydrolysis. A simplified kinetic model was used to fit the experimental data and predict the effects of substrate and enzyme intermittent feedings in fed-batch hydrolysis experiments. The proposed fed-batch procedure was found to significantly increase GlcEq productivity compared to batch experiments at 20 wt%.
Article
Green & Sustainable Science & Technology
Wen Wang, Mengxuan Zhang, Shijun Liu, Qiong Wang, Yunzi Hu, Cuiyi Liang, YunYun Liu, Hui Liu, Wei Qi
Summary: High energy consumption is a major obstacle for the conversion of lignocellulose into biofuels and biochemicals. In this study, a self-designed pilot-scale device with recycling of black liquor and waste washing water was used for low-temperature pretreatment and high-solid enzymatic hydrolysis of sugarcane bagasse. The results showed that the enzymatic hydrolysis of the treated sugarcane bagasse achieved a high glucose concentration and glucan conversion. A low-energy strategy for cellulosic ethanol production with low emission was proposed.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agricultural Engineering
Chao Xu, Yaru Xiong, Jun Zhang, Kuntai Li, Saiyi Zhong, Shushi Huang, Chunliang Xie, Wenbing Gong, Zuohua Zhu, Yingjun Zhou, Yuande Peng
Summary: This study applied response surface methodology to optimize the liquid hot water pretreatment of sugarcane bagasse, and achieved the highest sugar yield under the pretreatment conditions of 186°C, 25 min, and solid-to-liquid ratio of 0.08. By enzymatic hydrolysis of the pretreated residuals at 20% (w/v) solid content, the obtained sugars reached 121 g/L. Through controlling the fermentation process, most of the C5 and C6 sugars were converted into succinic acid with a high conversion rate of 280 mg/g SCB. This study provides a new clue for clean and efficient biorefining of chemicals.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Qingfeng Wang, Xuesong Tan, Wen Wang, Changlin Miao, Yongming Sun, Zhenhong Yuan, Xinshu Zhuang
Summary: In this study, a cellulose ethanol production method was developed by alkaline pretreatment and enzymatic hydrolysis, which allows direct conversion of treated bagasse into ethanol. The method enables zero discharge of black liquor and wastewater, making it a potential candidate for industrial applications.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Luis Carlos Carvalho, Ana L. S. Oliveira, Erdem Carsanba, Manuela Pintado, Carla Oliveira
Summary: This study investigated the transfer of phenolic compounds from sugarcane syrup to the broth during beta-farnesene fermentation. The concentrations of most phenolic compounds increased until day 3 of fermentation and then remained constant. The Weibull model showed a better fit to the data.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Hemansi, Jitendra Kumar Saini
Summary: This study investigates the high gravity fed-batch simultaneous saccharification and fermentation (FB-SSF) of sequentially pretreated sugarcane bagasse (SCB) for enhanced bioethanol production, using the inhibitor tolerant Kluyveromyces marxianus JKH5 C60. The optimized lab-scale FB-SSF, with intermittent feeding of SCB and enzyme, resulted in maximum ethanol titer, efficiency, and productivities of 73.4 g/L, 78%, and 3.0 g/L/h, respectively, after 72 h in the presence of inhibitors. Additionally, fermentation of pentose rich dilute acid hydrolysate of SCB by Pichia stipitis NCIM 3499 yielded an ethanol titer of 6.8 g/L. The overall ethanol yield during the developed process was 260.1 g/kg native SCB, indicating the industrial potential of this bioethanol conversion process.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Juliana P. Sandri, Julen Ordenana, Thais S. Milessi, Teresa C. Zangirolami, Solange I. Mussatto
Summary: This study focuses on a feeding strategy to enhance high solid load enzymatic hydrolysis of sugarcane bagasse and sugar co-fermentation using a non-recombinant yeast co-culture. The findings provide significant contribution to improving the feasibility of second-generation biomass energy production.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Biochemistry & Molecular Biology
Eva Balaguer Moya, Berta Syhler, Julen Ordenana Manso, Giuliano Dragone, Solange I. Mussatto
Summary: This study evaluated the optimization of enzymatic hydrolysis cocktail to maximize sugar extraction from pretreated sugarcane bagasse. It was found that adding hydrogen peroxide at the beginning of hydrolysis increased glucose and xylose concentrations, while the addition of hemicellulase increased glucose and xylose production. These findings suggest that using an appropriate enzymatic cocktail supplemented with additives can enhance sugar extraction from lignocellulosic biomass and contribute to the development of a more sustainable and competitive process.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agricultural Engineering
Jiajie Zhang, Jun Xie, Hongdan Zhang
Summary: The NaOH catalytic ethanol pretreatment of sugarcane bagasse improved glucose yield by assisting delignification and preserving cellulose and hemicellulose. Higher temperature and longer time resulted in higher glucose yield, while the addition of Tween 80 significantly reduced enzyme usage and enzymolysis time while maintaining comparable glucose yield. This study provided an economically feasible method for generating glucose followed by fermentation and conversion to platform chemicals.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Rongxin Ling, Weiqi Wei, Yongcan Jin
Summary: This study explored the effect of HCl/EG-H2O pretreatment on the enzymatic conversion of cellulose in sugarcane bagasse. The results showed that the selective extraction of xylan and lignin can be achieved while maintaining high cellulose retention. The addition of enzymatic additives improved hydrolysis efficiency and reduced enzyme dosage. Furthermore, a linear relationship between substrate characteristic parameters and cellulose conversion was observed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Fernando Roberto Paz-Cedeno, Lucas Ragnini Henares, Eddyn Gabriel Solorzano-Chavez, Mateus Scontri, Flavio Pereira Picheli, Ismael Ulises Miranda Roldan, Rubens Monti, Samuel Conceicao de Oliveira, Fernando Masarin
Summary: The study showed that pretreatment with sulfite-NaOH was the most efficient in removing lignin while keeping cellulose intact, and had the best response to enzymatic hydrolysis with high conversion rates.
Article
Agricultural Engineering
Si Lu, Qiong Wang, Zheng Liang, Wen Wang, Cuiyi Liang, Zhongming Wang, Zhenhong Yuan, Ping Lan, Wei Qi
Summary: A sustainable hydrolysis method combined with catalytic pretreatment and enzymatic hydrolysis was applied to enhance reducing sugar production from sugarcane bagasse. The optimized conditions resulted in high xylose and glucose yields, as well as significantly improved enzymatic digestibility compared to natural sugarcane bagasse.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Jantima Arnthong, Piyada Bussadee, Apisan Phienluphon, Pacharawan Deenarn, Kan Tulsook, Sa-ngapong Plupjeen, Chatuphon Siamphan, Chakrit Tachaapaikoon, Verawat Champreda, Surisa Suwannarangsee
Summary: This study investigates the improvement of cellulase activity and ethanol production by testing different genes involved in glycosylphosphatidylinositol (GPI) biosynthesis and remodeling. The overexpression of LAS21 gene is found to significantly improve beta-glucosidase activity. The engineered strain shows enhanced ethanol yield through further optimization of the bioprocess parameters. This study provides a promising approach to improve the economic viability of second-generation ethanol production.
FERMENTATION-BASEL
(2022)
Article
Agricultural Engineering
Nicolas Ramirez, Claudia Ubilla, Javiera Campos, Francisca Valencia, Carla Aburto, Carlos Vera, Andres Illanes, Cecilia Guerrero
Summary: The study evaluated the effects of operational variables on lactulose production by enzymatic transgalactosylation in fed-batch and repeated fed-batch. Feed flowrate and fructose to lactose molar ratio were the most significant variables affecting lactulose yield, productivity, and selectivity. The repeated fed-batch operation increased efficiency of biocatalyst use and accumulated productivity significantly compared to batch and fed-batch operations with the same biocatalyst.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Leila Khaleghipour, Javier A. Linares-Pasten, Hamid Rashedi, Seyed Omid Ranaei Siadat, Andrius Jasilionis, Said Al-Hamimi, Roya R. R. Sardari, Eva Nordberg Karlsson
Summary: An integrated scheme was developed for successful separation of sugarcane bagasse xylan, lignin, and cellulose. Higher concentration of alkali resulted in xylan with lower degree of substitution, while high pressure conditions released more lignin than xylan. Enzymatic hydrolysis was more efficient using xylan extracted at lower alkaline strength.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
G. J. M. Rocha, L. P. Andrade, C. Martin, G. T. Araujo, V. E. Mouchrek Filho, A. A. S. Curvelo
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Article
Biochemistry & Molecular Biology
Alfredo Oliva-Taravilla, Cristhian Carrasco, Leif J. Jonsson, Carlos Martin
Article
Biochemical Research Methods
Anderson Jose Paulo, Maria Carolina de Albuquerque Wanderley, Rafael Jose Vilela de Oliveira, Willie Anderson dos Santos Vieira, Luiz Carlos Alves, Daniela de Araujo Viana Marques, Attilio Converti, Ana Lucia Figueiredo Porto
Summary: The production, partial purification, and characterization of beta-Galactosidase by a new fungal isolate were investigated. Partial purification was achieved through an aqueous two-phase system (ATPS), resulting in high yield and purification factor. The enzyme showed optimal activity under specific temperature and pH conditions, making it suitable for prebiotics production.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2021)
Article
Agronomy
Dimitrios Ilanidis, Stefan Stagge, Leif J. Jonsson, Carlos Martin
Summary: Biochemical conversion of wheat straw was studied using hydrothermal pretreatment, enzymatic saccharification, and microbial fermentation. Different pretreatment conditions had significant effects on chemical composition, enzymatic digestibility, carbohydrate composition, inhibitory byproducts, furfural, and fermentability. Xylose concentration in pretreatment liquids increased with temperature up to 190 degrees C and then decreased.
Editorial Material
Agronomy
Carlos Martin
Review
Biotechnology & Applied Microbiology
Helena Chacon-Navarrete, Carlos Martin, Jaime Moreno-Garcia
Summary: Immobilizing yeast with low-cost carrier materials can optimize the fermentation of lignocellulosic hydrolysates for second-generation ethanol production. Yeast immobilization enhances fermentation performance, resistance to inhibitors, co-utilization of sugars, separation operations, and yeast reuse. Alternative carrier materials such as biochar and multispecies biofilm membranes show promise as well.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2021)
Article
Energy & Fuels
Cristhian Carrasco, Leif J. Jonsson, Carlos Martin
Summary: Extraction treatment can reduce the recalcitrance of quinoa stalks towards enzymatic saccharification, leading to higher glucan recovery and lower hemicellulose hydrolysis during pretreatment, as well as reduced by-product formation. Pre-extraction enhances glucan susceptibility towards enzymatic hydrolysis, resulting in higher overall conversion values.
Article
Biochemical Research Methods
Jose Manoel Wanderley Duarte Neto, Jonatas de Carvalho Silva, Flavia Sousa, Odete Sofia Lopes Goncalves, Maria Carolina de Albuquerque Wanderley, Bruno Sarmento, Carolina de Albuquerque Lima, Maria Teresa Neves-Petersen, Ana Lucia Figueiredo Porto
Summary: This study characterized a stable extracellular alkaline peptidase produced by Penicillium aurantiogriseum through various methods, revealing its structural and stability characteristics at different pH values. The findings suggest that the protease exhibits higher thermal stability and alpha-helix content at pH 9.0, indicating its potential applicability in practical production.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Editorial Material
Energy & Fuels
Cigdem Yangin-Gomec, Ilona Sarvari Horvath, Carlos Martin
Editorial Material
Biochemistry & Molecular Biology
Alejandro Rodriguez, Eduardo Espinosa, Carlos Martin
Article
Biochemistry & Molecular Biology
Sarah J. Klausen, Anne Bergljot Falck-Ytter, Knut Olav Straetkvern, Carlos Martin
Summary: The extraction of bioactive compounds and cellulose saccharification from spent mushroom substrate (SMS) is a potential way for resource utilization. This study investigated the suitability of different extraction methods for recovering bioactive compounds from SMS and their impact on enzymatic saccharification. Results showed that a 40:60 ethanol/water mixture in Soxhlet extraction had the highest extraction efficiency (EE) (69.9-71.1%). Reflux extraction with 40:60 ethanol/water increased the extraction yield and EE compared to Soxhlet extraction. Ultrasound-assisted extraction at 65 degrees C for 60 min allowed for a higher EE (around 82%) than reflux extraction for up to 150 min or Soxhlet extraction.
Review
Biotechnology & Applied Microbiology
Carlos Martin, Georgios I. Zervakis, Shaojun Xiong, Georgios Koutrotsios, Knut Olav Straetkvern
Summary: Spent mushroom substrate (SMS), the residual biomass after harvesting edible/medicinal fungi, poses significant environmental and financial challenges. Recycling and valorization of SMS are crucial for the sustainable development of the mushroom industry within the circular economy principles. This review critically discusses the potential applications of SMS in plant and mushroom cultivation, animal husbandry, and recovery of enzymes and bioactive compounds, based on its physical properties and chemical composition.
Review
Agronomy
Eulogio Castro, Knut Olav Straetkvern, Juan Miguel Romero-Garcia, Carlos Martin
Summary: The traditional biodiesel production model using edible oils as raw materials is unsustainable due to high costs, competition with the food sector, and environmental threats. Shifting to non-edible oils as raw materials for biodiesel production offers the opportunity for increased sustainability and avoiding conflicts with food production. Processing non-edible oilseeds generates large amounts of residues, which can be upgraded to advanced biofuels and high-value products through bioconversion. However, pretreatment is required to enhance the susceptibility of these residues to enzymes and microorganisms.
Article
Biotechnology & Applied Microbiology
Vidal Flores-Copa, Luis Romero-Soto, Danitza Romero-Calle, Maria Teresa Alvarez-Aliaga, Felipe Orozco-Gutierrez, Jose Vega-Baudrit, Carlos Martin, Cristhian Carrasco
Summary: The study showed that aeration is crucial for the production of biomass and biofilm, while agitation only affects biofilm production. The optimal biofilm production was achieved at an agitation speed of 200 rpm and an aeration of 1 L min(-1).
FERMENTATION-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Diego Chambi, Luis Romero-Soto, Roxana Villca, Felipe Orozco-Gutierrez, Jose Vega-Baudrit, Jorge Quillaguaman, Rajni Hatti-Kaul, Carlos Martin, Cristhian Carrasco
Summary: The halotolerant bacterial strain BU-4 was identified as an exopolysaccharide (EPS) producer isolated from a hypersaline environment. This strain was able to produce EPS from pretreatment liquids and enzymatic hydrolysates, with high thermal stability, water retention capacity, and gel-forming ability, showcasing its broad application potential.
FERMENTATION-BASEL
(2021)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
