Article
Biotechnology & Applied Microbiology
Heung-Soon Park, Ji-Hee Park, Hye-Jin Kim, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
Summary: This study isolated a Streptomyces strain with strong antifungal activity and identified a large biosynthetic gene cluster (BGC) for a polyene compound. Overexpression of the BGC in the strain and heterologous expression in another strain led to increased production of the compound. The researchers also created strains lacking the sugar moiety of the compound and found that the sugar played a critical role in its biological activity. BAC cloning of large natural product BGCs in Streptomyces is a valuable approach for improving production and screening for biological activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sofia Melnyk, Anastasia Stepanyshyn, Oleksandr Yushchuk, Michael Mandler, Iryna Ostash, Oksana Koshla, Victor Fedorenko, Daniel Kahne, Bohdan Ostash
Summary: This article presents the expansion of the genetic toolkit for Streptomyces roseochromogenes NRRL 3504 by transferring integrative and replicative vectors, and increasing clorobiocin production using a cumate-inducible expression system.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Soonkyu Hwang, Yongjae Lee, Ji Hun Kim, Gahyeon Kim, Hyeseong Kim, Woori Kim, Suhyung Cho, Bernhard O. Palsson, Byung-Kwan Cho
Summary: The production of recombinant proteins from different organisms is becoming more popular in biotechnology due to its advantages in productivity, scalability, and versatility. Streptomyces species are suggested as excellent hosts for this purpose, but their use is limited by low yield, genetic manipulation challenges, and cellular complexity. This review focuses on rational engineering approaches to optimize heterologous production in Streptomyces, including genetic tool development and chassis construction, with the aim of increasing availability of secondary metabolites and recombinant proteins.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Organic
Mingming Yu, Jianying Luo, Dan Luo, Qiang He, Yijun Yan, Xu Ji, Sheng-Xiong Huang
Summary: Genome mining led to the isolation of sarubicins A and B with rare structures, along with three new C-glycosylated quinazolinones. These compounds showed potent protective effects on neonatal rat cardiomyocytes.
ORGANIC CHEMISTRY FRONTIERS
(2021)
Review
Chemistry, Multidisciplinary
Van Thuy Thi Pham, Chung Thanh Nguyen, Dipesh Dhakal, Hue Thi Nguyen, Tae-Su Kim, Jae Kyung Sohng
Summary: Streptomyces is an important source of natural products used in therapeutic antibiotics, anticancer agents, pesticides, and dyes. Advances in metabolite analysis have led to the characterization of many new secondary metabolites. Techniques like OSMAC, overexpression/deletion of regulatory genes, ribosome engineering, and promoter replacement have been utilized to enhance the production titer of target compounds. Heterologous expression of BGCs has successfully been employed for the detection, characterization, and production of many secondary metabolites.
APPLIED SCIENCES-BASEL
(2021)
Article
Biology
Xuejin Zhao, Yeqing Zong, Weijia Wei, Chunbo Lou
Summary: This study presents a method for recombining the thaxtomin A gene cluster and improving its yield by obtaining high-yielding Streptomyces hosts. Additionally, an efficient gene editing method based on CRISPR/Cas9 digestion and yeast homologous recombination was developed. Results showed that by replacing promoters and optimizing gene cluster structure, the yield of thaxtomin A was significantly improved.
Article
Agriculture, Multidisciplinary
Yu Li, Zifei Xu, Ping Chen, Chen Zuo, Liyifan Chen, Wei Yan, Ruihua Jiao, Yonghao Ye
Summary: A type II polyketide synthase biosynthetic gene cluster (nap) was found in Streptomyces eurocidicus CGMCC 4.1086 through genome mining. Heterologous expression of the nap gene cluster in Streptomyces albus J1074 produced dimerized aromatic polyketides naphthocyclinones (1-3), which were structurally characterized by extensive analysis. The synthesis pathway of naphthocyclinones was revealed through genetic manipulation, biochemical reactions, and comparative genomics. Remarkably, compound 3 exhibited strong inhibitory effects against Phytophthora capsici and Phytophthora sojae, showing promising potential for plant disease control.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Shumao Chai, Mingming Cao, Zhiyang Feng
Summary: Nitrogen heterocycle small molecules exhibit various pharmaceutically important bioactivities and have great potential in drug development and application. Microbes serve as an important source for discovering nitrogen heterocycle natural products, and this study uncovered a new biosynthetic machinery in Streptomyces for producing nitrogen heterocycle natural products.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Plant Sciences
Han Wang, Huan Qi, Shao-Yong Zhang, Wen-Shuai Song, Li-Qin Zhang, Wen-Sheng Xiang, Ji-Dong Wang
Summary: A chemical investigation of Streptomyces sp. Hu186 led to the discovery of known quinone antibiotics and new metabolites with unique structures, which showed moderate cytotoxic activity against tumor cell lines.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Microbiology
Kohta Unno, Shinya Kodani
Summary: Recently, a new biosynthetic gene cluster of co-ester-containing peptides (OEPs) was discovered in the genome of actinobacterium Streptomyces prunicolor. A new OEP named prunipeptin was successfully produced through heterologous co-expression of precursor peptide and ATP-grasp ligase genes. Further mutagenesis experiment resulted in a variant peptide prunipeptin 15HW, and the bridging pattern of this peptide was determined through chemical cleavage and MS experiments, indicating the catalytic function of ATP-grasp ligase in forming intramolecular bonds.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Integrative & Complementary Medicine
Qiushuang Xu, Haochen Zou, Chen Pan, Haoxin Wang, Yuemao Shen, Yaoyao Li
Summary: In this study, a cryptic polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) gene cluster in Lysobacter sp. DSM 3655 was activated through promoter engineering and heterologous expression in Streptomyces sp. S001. Two novel linear lipopeptides, lysohexaenetides A and B, were successfully isolated. The biosynthetic pathway for lysohexaenetides was proposed, and LshA was identified as another example of entirely iterative bacterial PKSs. This study highlights the potential of heterologous expression systems in uncovering cryptic biosynthetic pathways in Lysobacter genomes, particularly in the absence of genetic manipulation tools.
CHINESE JOURNAL OF NATURAL MEDICINES
(2023)
Article
Integrative & Complementary Medicine
Wei Weijia, Wang Wenzhao, Li Chao, Tang Yue, Guo Zhengyan, Chen Yihua
Summary: In this study, a biosynthetic gene cluster for di-AFN A(1) was constructed and its production was increased through heterologous expression. The strategy of multiplying and splitting the di-AFN A(1) biosynthetic gene cluster resulted in a significant increase in di-AFN A(1) production.
CHINESE JOURNAL OF NATURAL MEDICINES
(2022)
Article
Plant Sciences
Shun Saito, Kayo Funayama, Wataru Kato, Mayu Okuda, Meiko Kawamoto, Teruhiko Matsubara, Toshinori Sato, Akihiko Sato, Satoko Otsuguro, Michihito Sasaki, Yasuko Orba, Hirofumi Sawa, Katsumi Maenaka, Kazutoshi Shindo, Masaya Imoto, Midori A. Arai
Summary: A new maniwamycin derivative, produced under high temperature conditions, demonstrates antiviral activity against both influenza and the SARS-CoV-2 virus. The structures of these compounds were elucidated through NMR and MS spectroscopic analyses.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Multidisciplinary Sciences
Evan M. F. Shepherdson, Marie A. Elliot
Summary: Streptomyces bacteria have complex life cycles and remarkable metabolic capabilities. Their recently discovered exploration ability allows them to successfully compete with other microbes. Nutrient availability affects exploration rates and metabolic output, with glycerol dramatically increasing exploration rates. Different siderophores play different roles in exploration, and the alternative siderophore foroxymithine is more important than desferrioxamine during coculture.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Anton P. Tyurin, Vera A. Alferova, Alexander S. Paramonov, Maxim V. Shuvalov, Gulnara K. Kudryakova, Eugene A. Rogozhin, Alexander Y. Zherebker, Vladimir A. Brylev, Alexey A. Chistov, Anna A. Baranova, Mikhail V. Biryukov, Igor A. Ivanov, Igor A. Prokhorenko, Natalia E. Grammatikova, Tatyana V. Kravchenko, Elena B. Isakova, Elena P. Mirchink, Elena G. Gladkikh, Elena V. Svirshchevskaya, Andrey V. Mardanov, Aleksey V. Beletsky, Milita V. Kocharovskaya, Valeriya V. Kulyaeva, Alexander S. Shashkov, Dmitry E. Tsvetkov, Nikolay E. Nifantiev, Alexander S. Apt, Konstantin B. Majorov, Svetlana S. Efimova, Nikolai V. Ravin, Evgeny N. Nikolaev, Olga S. Ostroumova, Genrikh S. Katrukha, Olda A. Lapchinskaya, Olga A. Dontsova, Stanislav S. Terekhov, Ilya A. Osterman, Zakhar O. Shenkarev, Vladimir A. Korshun
Summary: A novel family of natural lipoglycopeptides produced by Streptomyces sp. INA-Ac-5812, including gausemycins A and B, have been reported. These compounds exhibit pronounced activity against Gram-positive bacteria and show significant differences in mechanism compared to known glyco- and lipopeptides.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Franziska Wiker, Martin Konnerth, Irina Helmle, Andreas Kulik, Leonard Kaysser, Harald Gross, Bertolt Gust
ACS CHEMICAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Simon Schaefermann, Richard Neci, Edward Ngah Ndze, Fidelis Nyaah, Valentin Basolanduma Pondo, Lutz Heide
Article
Public, Environmental & Occupational Health
Simon Schaefermann, Cathrin Hauk, Emmanuel Wemakor, Richard Neci, Georges Mutombo, Edward Ngah Ndze, Tambo Cletus, Fidelis Nyaah, Manyi Pattinora, Dorothee Wistuba, Irina Helmle, Christine Haefele-Abah, Harald Gross, Lutz Heide
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2020)
Article
Public, Environmental & Occupational Health
Gesa Gnegel, Cathrin Hauk, Richard Neci, Georges Mutombo, Fidelis Nyaah, Dorothee Wistuba, Christine Haefele-Abah, Lutz Heide
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2020)
Article
Biochemistry & Molecular Biology
Julia Chen, Andri Frediansyah, Daniel Maennle, Jan Straetener, Heike Broetz-Oesterhelt, Nadine Ziemert, Leonard Kaysser, Harald Gross
Article
Obstetrics & Gynecology
Nhomsai Hagen, Felix Khuluza, Lutz Heide
BMC PREGNANCY AND CHILDBIRTH
(2020)
Article
Microbiology
Daniel Maennle, Shaun M. K. McKinnie, Shrikant S. Mantri, Katharina Steinke, Zeyin Lu, Bradley S. Moore, Nadine Ziemert, Leonard Kaysser
Article
Public, Environmental & Occupational Health
Nhomsai Hagen, Thomas Bizimana, Pierre Claver Kayumba, Felix Khuluza, Lutz Heide
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2020)
Article
Multidisciplinary Sciences
Thomas Bizimana, Pierre Claver Kayumba, Lutz Heide
Editorial Material
Public, Environmental & Occupational Health
Paul N. Newton, Katherine C. Bond
LANCET GLOBAL HEALTH
(2020)
Article
Multidisciplinary Sciences
Nhomsai Hagen, Thomas Bizimana, P. Claver Kayumba, Felix Khuluza, Lutz Heide
Article
Biotechnology & Applied Microbiology
Juan Pablo Gomez-Escribano, Neil A. Holmes, Susan Schlimpert, Maureen J. Bibb, Govind Chandra, Barrie Wilkinson, Mark J. Buttner, Mervyn J. Bibb
Summary: Streptomyces venezuelae has been widely used as a model strain for studying molecular mechanisms controlling morphological development in streptomycetes. Its rapid growth and ability to sporulate in a near-synchronized manner in liquid culture make it suitable for modern molecular techniques. A high-quality genome sequence of the strain was described, revealing differences in spore pigmentation and the successful use of CRISPR-Cas9 to cure the plasmid without noticeable phenotypic consequences.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Juan Pablo Gomez-Escribano, Lis Algora Gallardo, Kenan A. J. Bozhuyuk, Steven G. Kendrew, Benjamin D. Huckle, Nicola A. Crowhurst, Mervyn J. Bibb, Andrew J. Collis, Jason Micklefield, Paul R. Herron, Barrie Wilkinson
Summary: Streptomyces clavuligerus is an industrially important actinomycete with genetic manipulation limitations. Through Cas9-mediated genome editing, successful curing of pSCL4 revealed its crucial role in the structural stability of the linear chromosome.
MICROBIAL GENOMICS
(2021)
Review
Biochemistry & Molecular Biology
Leonard Kaysser
NATURAL PRODUCT REPORTS
(2019)
Article
Chemistry, Multidisciplinary
Alexandra Baulig, Irina Helmle, Marius Bader, Felix Wolf, Andreas Kulik, Arwa Al-Dilaimi, Daniel Wibberg, Joern Kalinowski, Harald Gross, Leonard Kaysser