Article
Biochemistry & Molecular Biology
Jae Min Shin, Moon Soo Han, Jae Hyung Park, Seung Hyeok Lee, Tae Hoon Kim, Sang Hag Lee
Summary: This study aimed to investigate the role of ephrinA1/ephA2 signaling in epithelial barrier integrity and rhinovirus-induced epithelial permeability in chronic rhinosinusitis (CRS). The results showed that ephrinA1 treatment increased epithelial permeability and decreased the expression of ZO-1, ZO-2, and occludin. Blocking ephA2 attenuated these effects. Rhinovirus infection upregulated ephrinA1 and ephA2 expression, leading to increased epithelial permeability, which was suppressed in ephA2-deficient cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Albert Ptashnik, Nicole LaMassa, Aliya Mambetalieva, Emily Schnall, Mike Bucaro, Greg R. Phillips
Summary: The family of around 60 clustered protocadherins (Pcdhs) is a group of cell adhesion molecules encoded by a specific gene locus. These molecules regulate the expression of different isoforms in individual neurons, resulting in a neural surface barcode that mediates interactions between cells. Same-cell interactions mediated by Pcdhs lead to avoidance, while interactions between different cells through Pcdhs are more stable. The cellular mechanism behind Pcdh-based adhesion is not well understood, but recent studies have uncovered various signaling pathways. The role of an unidentified cytoplasmic regulatory mechanism in the switch between avoidance and adhesion is proposed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Daniel Dar, Nina Dar, Long Cai, Dianne K. Newman
Summary: The par-seqFISH method allows for recording gene expression and spatial context of microbial populations, revealing diverse physiological states coexisting within the same biofilm with high spatial resolution when studying Pseudomonas aeruginosa.
Article
Biochemical Research Methods
Dylan M. Cable, Evan Murray, Vignesh Shanmugam, Simon Zhang, Luli S. Zou, Michael Diao, Haiqi Chen, Evan Z. Macosko, Rafael A. Irizarry, Fei Chen
Summary: A central problem in spatial transcriptomics is detecting differentially expressed genes within cell types across tissue context. This study introduces a statistical method called cell type-specific inference of differential expression (C-SIDE), which identifies cell type-specific DE and provides valid uncertainty quantification.
Article
Cell Biology
Laura Hakanpaa, Amr Abouelezz, An-Sofie Lenaerts, Seyda Culfa, Michael Algie, Jenny Barlund, Pekka Katajisto, Harvey McMahon, Leonardo Almeida-Souza
Summary: Hakanpaa et al. discovered that reticular adhesions are formed at flat clathrin lattices, a process inhibited by fibronectin and its receptor integrin alpha 5 beta 1. This novel adhesion assembly mechanism is involved in cell migration and reveals a unique crosstalk between cell-matrix adhesions.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yoko Ito, Nicolas Esnay, Matthieu Pierre Platre, Valerie Wattelet-Boyer, Lise C. Noack, Louise Fougere, Wilhelm Menzel, Stephane Claverol, Laetitia Fouillen, Patrick Moreau, Yvon Jaillais, Yohann Boutte
Summary: This study uncovers a mechanism in plant cells where sphingolipids mediate phosphoinositide homeostasis at the TGN, shedding light on the role of lipids in protein sorting.
NATURE COMMUNICATIONS
(2021)
Article
Materials Science, Multidisciplinary
Wenguang Yang, Honghui Chu, Shuxiang Cai, Wenfeng Liang, Haibo Yu, Yuechao Wang, Lianqing Liu
Summary: A flexible and practical method is proposed to construct a cell-repellent interface and engineer controlled cell organization. Using femtosecond-laser direct writing to pattern surfaces with microcraters, micropatterned surfaces with super-hydrophobicity characteristics are achieved, inhibiting cell adhesion and enabling arbitrary cell patterns through selective cell adhesion induced by the distribution of the microcrater structures.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Multidisciplinary Sciences
Audun Kvalvaag, Salvatore Valvo, Pablo F. Cespedes, David G. Saliba, Elke Kurz, Kseniya Korobchevskaya, Michael L. Dustin
Summary: Ligation of TCR to pMHC induces TCR ubiquitination and either internalization by T cell or release in extracellular vesicles. Clathrin-mediated ectocytosis facilitates the release of TCR, while clathrin-mediated trans-endocytosis enables the uptake of pMHC-TCR conjugates from the APC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Anish R. Roy, Wei Zhang, Zeinab Jahed, Ching-Ting Tsai, Bianxiao Cui, W. E. Moerner
Summary: The topography of the plasma membrane has a strong influence on cellular processes. Recent studies have used 3D nanostructures to study the effects of membrane curvature on protein distribution. In this study, a method combining silicone-oil immersion objective and 3D double-helix point spread function microscopy is introduced to investigate the nanoscale distribution of proteins at the nano-bio interface.
Article
Chemistry, Physical
Hayri E. Balcioglu, Rolf Harkes, Erik H. J. Danen, Thomas Schmidt
Summary: This study used quantitative super-resolution microscopy to investigate cell-matrix adhesions and found that there is a quantitative relationship between cellular traction force and the number of cell-matrix adhesion proteins. The stoichiometry of these proteins changes with different substrate stiffness, indicating a substrate-stiffness-dependent modulation of cell-matrix adhesion.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Multidisciplinary Sciences
Fabian R. Villagomez, Juan D. Diaz-Valencia, Erasmo Ovalle-Garcia, Armando Antillon, Ivan Ortega-Blake, Hector Romero-Ramirez, Jorge F. Cerna-Cortes, Roberto Rosales-Reyes, Leopoldo Santos-Argumedo, Genaro Patino-Lopez
Summary: Cell spreading and phagocytosis are regulated by small GTPases and GAP proteins, with TBC1D10C identified as a dual inhibitory protein with GAP activity. TBC1D10C modulates lymphocyte activation in immune cells, and our study reveals its involvement in reorganizing the cytoskeleton and plasma membrane of macrophages, impacting cell spreading and phagocytic activity.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Houbing Zou, Hu Shi, Shijiao Hao, Yajuan Hao, Jie Yang, Xinxin Tian, Hengquan Yang
Summary: In this study, the oil-water interfaces of Pickering emulsions were utilized to regulate catalytic selectivity of hydrogenation reactions by controlling the spatial distribution of metal nanoparticles at the droplet interfaces. The remarkable interfacial microregion-dependent catalytic selectivity was found to be attributed to the microenvironments of the coexistence of water and organic solvent at the droplet interfaces, altering the adsorption patterns of the reactants on the metal nanoparticles. This strategy provides new insights into liquid-liquid interfacial reactions and offers a way to enhance catalytic selectivity.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Microbiology
Aaron S. Dhanda, A. Wayne Vogl, Fern Ness, Metello Innocenti, Julian A. Guttman
Summary: Direct cell-to-cell spreading of Listeria monocytogenes requires the induction of actin-based membrane protrusions in donor cells, which are endocytosed by adjacent receiving cells. Formin mDia1 plays a vital role in the assembly of the F-actin shell during this process, leading to efficient cell-to-cell transfer of the bacteria.
Article
Plant Sciences
Joel Oliver, Mingzhu Fan, Brian McKinley, Starla Zemelis-Durfee, Federica Brandizzi, Curtis Wilkerson, John E. Mullet
Summary: The mutation of Dw2 kinase in bioenergy sorghum affects cell proliferation in internode intercalary meristems, endocytosis, and the distribution of key components in cell walls. Additionally, Dw2 signaling influences the phosphorylation of proteins related to lipid signaling, hormones, and photosynthesis.
Article
Biochemistry & Molecular Biology
Zhiwei Fan, Yangyang Luo, Huifen Lu, Tiangang Wang, YuZhou Feng, Weiling Zhao, Pora Kim, Xiaobo Zhou
Summary: Spatial transcriptomics is a promising technology that provides information about spatial heterogeneity in tissue architecture. However, existing spatial transcriptomics databases have limited analytical information. In this study, a new database called SPASCER was constructed to help researchers understand tissue heterogeneity and intercellular interactions. The integration of scRNA-seq data and analysis of cell-cell interactions and gene regulation networks in spatial transcriptomics were performed. The application of SPASCER will provide insights into tissue architecture and contribute to the mechanistic understanding of biological processes in healthy and diseased tissues.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Lillian K. Fritz-Laylin, Samuel J. Lord, R. Dyche Mullins
JOURNAL OF CELL BIOLOGY
(2017)
Article
Biology
Lillian K. Fritz-Laylin, Megan Riel-Mehan, Bi-Chang Chen, Samuel J. Lord, Thomas D. Goddard, Thomas E. Ferrin, Susan M. Nicholson-Dykstra, Henry Higgs, Graham T. Johnson, Eric Betzig, R. Dyche Mullins
Editorial Material
Biochemistry & Molecular Biology
Lillian K. Fritz-Laylin, Samuel J. Lord, Mallory Kakley, R. Dyche Mullins
Article
Biophysics
Hiroyuki Kai, Wataru Suda, Shotaro Yoshida, Matsuhiko Nishizawa
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Engineering, Electrical & Electronic
Kiichi Niitsu, Atsuki Kobayashi, Yuya Nishio, Kenya Hayashi, Kei Ikeda, Takashi Ando, Yudai Ogawa, Hiroyuki Kai, Matsuhiko Nishizawa, Kazuo Nakazato
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS I-REGULAR PAPERS
(2018)
Article
Chemistry, Multidisciplinary
Hiroyuki Kai, Ryoma Toyosato, Matsuhiko Nishizawa
Article
Multidisciplinary Sciences
Kuniaki Nagamine, Hirotaka Sato, Hiroyuki Kai, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa
SCIENTIFIC REPORTS
(2018)
Article
Biochemical Research Methods
Bin Yang, Xingye Chen, Yina Wang, Siyu Feng, Veronica Pessino, Nico Stuurman, Nathan H. Cho, Karen W. Cheng, Samuel J. Lord, Linfeng Xu, Dan Xie, R. Dyche Mullins, Manuel D. Leonetti, Bo Huang
Editorial Material
Cell Biology
Samuel J. Lord, Katrina B. Velle, R. Dyche Mullins, Lillian K. Fritz-Laylin
JOURNAL OF CELL BIOLOGY
(2020)
Article
Microbiology
Arthur Charles-Orszag, Samuel J. Lord, R. Dyche Mullins
Summary: The study overcame significant technical challenges in extremophile cell biology by using a newly developed imaging system that allowed observation and quantification of cell division dynamics in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius with unprecedented resolution. This system also revealed previously undescribed dynamic cell shape changes, cell motility, and cell-cell interactions, shedding new light on the high-temperature lifestyle of this organism.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Shunsuke Yamamoto, Hiroyuki Kai
Summary: Organic-inorganic hybrid films based on TiO2 and a fluorinated polymer were fabricated and their properties were investigated. The preparation process and UV irradiation were found to have significant effects on the surface properties. These hybrid films have been used as substrates in biosensing applications, allowing the collection and analysis of body fluids on a single platform.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Yusho Segawa, Kentaro Kinoshita, Hiroyuki Kai
Summary: This study develops open microfluidic channels with hyperbranched, space-filling structures, which enable the manipulation of liquid on the open surface. These channels efficiently capture, transport, and collect microdroplets of low surface tension liquid, expanding the possibilities for advancements in the field of open microfluidics.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Energy & Fuels
Hiroyuki Kai, Akichika Kumatani
Summary: Porous microneedle electrodes with pores of approximately 1 μm diameter were fabricated and exhibited a specific surface area of 2.559 m(2) g(-1), higher than that of the non-porous control. Experimental results showed that porous structures in electrodes have advantages in electrochemical sensing with increased sensitivity.
JOURNAL OF PHYSICS-ENERGY
(2021)
Article
Chemistry, Analytical
Hiroyuki Kai, Yuto Kato, Ryoma Toyosato, Matsuhiko Nishizawa
Article
Engineering, Biomedical
Kuniaki Nagamine, Jun Kubota, Hiroyuki Kai, Yoshinobu Ono, Matsuhiko Nishizawa
BIOMEDICAL MICRODEVICES
(2017)