Article
Clinical Neurology
Daniela Dumitriu LaGrange, Vincent Braunersreuther, Isabel Wanke, Jatta Berberat, Siri Luthman, Sean Fitzgerald, Karen M. Doyle, Olivier Brina, Philippe Reymond, Alexandra Platon, Michel Muster, Paolo Machi, Pierre-Alexandre Poletti, Maria Isabel Vargas, Karl-Olof Loevblad
Summary: BackgroundCharacterization of the clot occluding the arteries in acute ischemic stroke has received considerable attention, with efforts to elucidate the relationship between clot composition, etiology, and treatment approaches. Traditional 2D histopathology or electron microscopy techniques only sample small parts of the clot, highlighting the need for 3D visualization and analysis. A pilot study using microCT to analyze clots from 14 stroke patients found a heterogeneous distribution of intra-clot density, with a shift towards negative values after formalin fixation. Volumetric and density analysis offers new perspectives on clot characterization and understanding thrombus structure and composition.
FRONTIERS IN NEUROLOGY
(2022)
Article
Cell Biology
Masako Fujioka-Kobayashi, Michihide Kono, Hiroki Katagiri, Benoit Schaller, Yufeng Zhang, Anton Sculean, Richard J. Miron
Summary: Platelet-rich fibrin (PRF) was prepared from whole blood using two different centrifugation systems, fixed-angle and horizontal centrifugation. Results showed that the PRF clots produced by horizontal centrifugation had a more uniform blood cell separation compared to those produced by fixed-angle centrifugation. Future research is needed to evaluate the advantage of horizontal centrifugation in clinical practice.
Article
Biochemistry & Molecular Biology
Zahra Kargarpour, Layla Panahipour, Richard J. Miron, Reinhard Gruber
Summary: The aim of this study was to compare lysates prepared from platelet-rich fibrin (PRF) and unfractionated blood clot (UBC) based on bioassays and degradation of the respective membranes. The results showed that both PRF and UBC lysates had TGF-beta signaling activation and anti-inflammatory activity, but UBC membranes lacked structural integrity. Therefore, centrifugation separation is necessary to prepare durable and clinically applicable blood-derived membranes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Francisco Hernandez-Fernandez, Maria E. Ramos-Araque, Rosa Barbella-Aponte, Juan David Molina-Nuevo, Jorge Garcia-Garcia, Oscar Ayo-Martin, Maria Jose Pedrosa-Jimenez, Lorena Lopez-Martinez, Gemma Serrano-Heras, Enrique Julia-Molla, Tomas Segura
Summary: The histological composition of the clot can affect the efficacy of endovascular treatment for acute ischemic stroke (AIS). Fibrin-predominant clot patients were more likely to achieve complete recanalization when stent retrievers were used, but also had a higher mortality rate. CRP levels and the presence of HMCA were closely associated with clot composition.
FRONTIERS IN NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rustem Litvinov, Alina D. Peshkova, Giang Le Minh, Nail N. Khaertdinov, Natalia G. Evtugina, Guzel F. Sitdikova, John W. Weisel
Summary: Hyperhomocysteinemia (HHcy) is associated with enhanced blood clot contraction due to direct and indirect platelet activation. Higher levels of homocysteine can lead to impaired platelet function and larger, more obstructive clots.
Article
Engineering, Biomedical
Valerie Tutwiler, Farkhad Maksudov, Rustem Litvinov, John W. Weisel, Valeri Barsegov
Summary: Fibrin is crucial for the mechanical stability of blood clots and thrombi. This study investigates the rupture of blood clots by stretching fibrin gels with cracks and develops a Fluctuating Spring model to interpret stress-strain profiles. Cracks make network rupture more spontaneous, while coupled fibers enhance the stability of ruptured networks.
ACTA BIOMATERIALIA
(2021)
Article
Multidisciplinary Sciences
Yueyi Sun, Oluwamayokun Oshinowo, David R. Myers, Wilbur A. Lam, Alexander Alexeev
Summary: Blood clot contraction plays a crucial role in wound healing and hemostasis. This study investigates the relationship between single platelet forces and macroscopic clot forces, providing insights into blood disorders associated with bleeding and thrombosis. The findings contribute to the development of platelet-based biomaterials and facilitate the understanding of clotting mechanisms.
Article
Biochemistry & Molecular Biology
Xueqin Gao, Haizi Cheng, Xuying Sun, Aiping Lu, Joseph J. Ruzbarsky, Bing Wang, Johnny Huard
Summary: The study compared autologous blood clots and fibrin sealant as scaffolds for delivering lenti-BMP2/GFP-transduced hMDSCs for bone regeneration. It was found that autologous blood clot scaffold was less effective than fibrin sealant in promoting bone regeneration.
Review
Hematology
James I. Hearn, Elizabeth E. Gardiner
Summary: This article discusses the role of platelets in the formation of a hemostatic response and vascular injury repair, as well as their function in flowing blood. The article highlights the importance of platelet adhesion and activation in forming and strengthening adhesive ties, and discusses the challenges faced in research.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Hematology
Natalia G. Evtugina, Alina D. Peshkova, Alina I. Khabirova, Izabella A. Andrianova, Shahnoza Abdullayeva, Francis Ayombil, Taisia Shepeliuk, Ekaterina L. Grishchuk, Fazoil I. Ataullakhanov, Rustem I. Litvinov, John W. Weisel
Summary: The activation of Piezo1 in compressed RBCs during blood clot contraction leads to an increase in intracellular calcium concentration and affects platelet contractility. Adding the Piezo1 agonist Yoda1 to whole blood increases the extent of clot contraction, and this is achieved through enhanced generation of endogenous thrombin on activated RBCs.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biology
Christian Michael, Francesco Pancaldi, Samuel Britton, Oleg V. Kim, Alina D. Peshkova, Khoi Vo, Zhiliang Xu, Rustem I. Litvinov, John W. Weisel, Mark Alber
Summary: Little is known about the mechanisms underlying blood clot contraction, which plays a critical role in clot remodeling. Impairment of this process can lead to life-threatening bleeding and thrombotic conditions. A study on COVID-19 patients revealed hindered blood clot contraction, and a computational model was developed to understand the biomechanical mechanisms involved.
COMMUNICATIONS BIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Efrat Gerstman, Adi Hendler-Neumark, Verena Wulf, Gili Bisker
Summary: Blood coagulation is a crucial defense mechanism involving various clotting factors. This study demonstrates the use of near-infrared fluorescent single-walled carbon nanotubes to visualize and monitor fibrin clotting in real time. The platform offers improved signal-to-noise ratio and allows for the monitoring of coagulation cascade dynamics. It has potential applications in the diagnostics of coagulation disorders and real-time monitoring of the coagulation process.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Hematology
Gina Perrella, Jingnan Huang, Isabella Provenzale, Frauke Swieringa, Floor C. J. Heubel-Moenen, Richard W. Farndale, Mark Roest, Paola E. J. van der Meijden, Mark Thomas, Robert A. S. Ariens, Martine Jandrot-Perrus, Steve P. Watson, Johan W. M. Heemskerk
Summary: The study revealed that fibrin fibers play a consolidating role in microthrombus formation through nonredundant signaling of platelet glycoprotein VI and integrin alpha IIb beta 3 via Syk and low-level Ca2+ rises, even in the absence of thrombin, leading to minimal thrombin generation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Biochemical Research Methods
Laura Wolff-Trombini, Adrien Ceripa, Julien Moreau, Hubert Galinat, Chloe James, Nathalie Westbrook, Jean-Marc Allain
Summary: Hypercoagulability, a difficult-to-explain pathology, is likely caused by changes in fibrin polymerization conditions. Using passive microrheology, mechanical properties of clots were measured and correlated with structural information obtained from confocal microscopy. Known alterations, such as excess fibrinogen and coagulation Factor VIII, resulted in rigidification and densification of the fibrin network. Microrheology shows potential for hypercoagulability diagnosis.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Engineering, Mechanical
Dongjing He, Dongjune A. Kim, David N. Ku, Yuhang Hu
Summary: This study quantitatively characterizes the mechanical properties of blood clots using rheology and indentation measurements. The research finds that blood clots exhibit both viscoelastic and poroelastic behavior. The viscoelasticity is determined through shear rheology measurements, while the poroelastic properties are obtained through indentation tests. The study also discusses the contribution of solvent migration and viscoelastic effects to the time-dependent responses of blood clots.
EXTREME MECHANICS LETTERS
(2022)
Article
Hematology
M. Lehmann, K. Ashworth, M. Manco-Johnson, J. Di Paola, K. B. Neeves, C. J. Ng
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2018)
Article
Hematology
Marcus Lehmann, Rogier M. Schoeman, Patrick J. Krohl, Alison M. Wallbank, Joseph R. Samaniuk, Martine Jandrot-Perrus, Keith B. Neeves
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2018)
Article
Environmental Sciences
Kenton Rod, Wooyong Um, Jaehun Chun, Ning Wu, Xialong Yin, Guohui Wang, Keith Neeves
JOURNAL OF CONTAMINANT HYDROLOGY
(2018)
Article
Multidisciplinary Sciences
Kathryn G. Link, Michael T. Stobb, Jorge Di Paola, Keith B. Neeves, Aaron L. Fogelson, Suzanne S. Sindi, Karin Leiderman
Article
Robotics
Tao Yang, Andrew Tomaka, Tonguc O. Tasci, Keith B. Neeves, Ning Wu, David W. M. Marr
Editorial Material
Hematology
Hunter B. Moore, Keith B. Neeves
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2019)
Article
Chemistry, Analytical
Matthew G. Sorrells, Keith B. Neeves
Article
Instruments & Instrumentation
E. J. Roth, C. J. Zimmermann, D. Disharoon, T. O. Tasci, D. W. M. Marr, K. B. Neeves
REVIEW OF SCIENTIFIC INSTRUMENTS
(2020)
Article
Hematology
Robert C. Gosselin, Annette Bowyer, Emmanuel J. Favaloro, Jill M. Johnsen, Giuseppe Lippi, Richard A. Marlar, Keith Neeves, Marian A. Rollins-Raval
Summary: Recent shortage of 3.2% sodium citrate blood collection tubes has led stakeholders to seek suitable alternatives, with laboratories particularly concerned about variability between manufacturers and the challenges associated with transitioning to new blood collection methods. This provisional guidance document provides recommendations for validating alternative sources of sodium citrate blood collection tubes before clinical implementation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Dante Disharoon, Brian G. Trewyn, Paco S. Herson, David W. M. Marr, Keith B. Neeves
Summary: This study developed a platform for co-delivering tPA and plasminogen using microwheels to accelerate clot lysis, surpassing the biochemical speed limit through corkscrew motion. The results demonstrated significant efficacy in lysing plasma clots with this approach.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biotechnology & Applied Microbiology
Cara L. Sake, Alexander J. Metcalf, Michelle Meagher, Jorge Di Paola, Keith B. Neeves, Nanette R. Boyle
Summary: This study used isotopically nonstationary C-13 metabolic flux analysis to measure carbon fluxes in resting and activated platelets. The results showed significant differences in metabolic fluxes between activated and resting platelets, providing important insights into the mechanisms of platelet activation.
METABOLIC ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Matthew J. Osmond, Elizabeth Korthals, Coy J. Zimmermann, Eric J. Roth, David W. M. Marr, Keith B. Neeves
Summary: Inflammatory bowel disease (IBD) is caused by the over-expression of tumor necrosis factor-alpha (TNF) in the intestinal mucosa. Oral delivery of anti-TNF is hindered by degradation and poor bioavailability. To overcome these issues, researchers have developed magnetically powered hydrogel particles that can roll along mucosal surfaces, release anti-TNF, and repair damaged cell junctions. This method has the potential to be used for the treatment of IBD.
Article
Chemistry, Multidisciplinary
Aaron K. Ishiki, Keith B. Neeves, David W. M. Marr
Summary: High-molecular-weight polymers that approach the size scale of the gap between the microbots and surfaces can be excluded, impacting microbot transport. This exclusion leads to a reversal in the microbot translation direction.
Article
Biochemical Research Methods
Jess Berry, Francois J. Peaudecerf, Nicole A. Masters, Keith B. Neeves, Raymond E. Goldstein, Matthew T. Harper
Summary: Cardiovascular disease is a leading cause of death worldwide, with myocardial infarction being triggered by blood clots. Developing new anti-platelet drugs is an active area of research, with in vitro models using human blood showing potential in improving relevance. The study developed a microfluidic device for generating occlusive thrombi and demonstrated its ability to monitor the effect of anti-thrombotic drugs in an unbiased and objective manner.
Review
Hematology
Jessica Lin, Matthew G. Sorrells, Wilbur A. Lam, Keith B. Neeves
Summary: This illustrated review delves into the physical forces regulating hemostasis and thrombosis, spanning from vessel to cellular to molecular scales. Blood flow exerts forces on vessel walls and blood cells, impacting the kinetics, structure, and stability of thrombi. External forces and environmental stiffness play crucial roles in platelet function and clot formation.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2021)