Article
Biochemical Research Methods
Lara Dresser, Patrick Hunter, Fatima Yendybayeva, Alex L. Hargreaves, Jamieson A. L. Howard, Gareth J. O. Evans, Mark C. Leake, Steven D. Quinn
Summary: Alzheimer's disease is characterized by misfolding and aggregation of Aβ, with small soluble Aβ oligomers now recognized as more toxic than larger fibrillar aggregates. Techniques to characterize oligomer stoichiometry and assembly are crucial for understanding neurodegeneration. By integrating biophysics techniques, new insights into oligomer formation at the single-molecule level can be gained, offering potential for investigating other protein aggregation systems.
Article
Multidisciplinary Sciences
Fanjie Meng, Janghyun Yoo, Hoi Sung Chung
Summary: In this study, a method combining single-molecule fluorescence lifetime imaging and deep learning was developed to monitor individual fibril formation in real time and analyze them in a high-throughput manner. This method allows for quantitative characterization of highly heterogeneous fibril formation and the discovery of a common nucleation mechanism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Antonio Garcia, Shannon J. Saluga, David J. Dibble, Pia A. Lopez, Nozomi Saito, Suzanne A. Blum
Summary: This study for the first time imaged the selectivity of individual molecular catalysts for two different reactions in real time at the single-catalyst level, revealing that catalyst selectivity may be unexpectedly spatially and time-variable in ruthenium-catalyzed polymerization.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Jie Yang, Sarah Perrett, Si Wu
Summary: The misfolding and aggregation of polypeptide chains into amyloid fibrils are associated with neurodegenerative diseases. The oligomeric intermediates in early amyloid formation, rather than mature fibrils, are cytotoxic and potentially therapeutic targets. Single molecule fluorescence techniques have been developed to investigate amyloid oligomers and aggregation mechanisms.
Article
Biochemistry & Molecular Biology
Jacob Fritzsch, Alexander Korn, Dayana Surendran, Martin Krueger, Holger A. Scheidt, Kaustubh R. Mote, Perunthiruthy K. Madhu, Sudipta Maiti, Daniel Huster
Summary: Amyloid beta (A beta) is a peptide known to form amyloid fibrils in the brain of Alzheimer's disease patients. Mutations at positions Phe(20) or Gly(33) can lead to alterations in the fibrillation kinetics of A beta, as well as a severe decrease in cell toxicity compared to wildtype A beta(40). The region around the hydrophobic contact between Phe(19) and Leu(34) provides a promising site for designing small molecules to interfere with the A beta fibrillation pathway.
Article
Chemistry, Multidisciplinary
Nathan Meyer, Nicolas Arroyo, Jean-Marc Janot, Mathilde Lepoitevin, Anna Stevenson, Imad Abrao Nemeir, Veronique Perrier, Daisy Bougard, Maxime Belondrade, Didier Cot, Jeremy Bentin, Fabien Picaud, Joan Torrent, Sebastien Balme
Summary: This study investigates the impact of nanoscale geometry and molecular crowding on Aβ fibril sensing using nanopore technology. It was found that a conical nanopore functionalized with PEG 5 kDa can discriminate fibril size, while a bullet-like-shaped nanopore enhances current amplitude and dwell time. However, a nanopore crowded with PEG 20 kDa increases relative current blockade without improving fibril discrimination compared to the bullet-shaped nanopore.
Article
Chemistry, Multidisciplinary
Ewelina Stefaniak, Elena Atrian-Blasco, Wojciech Goch, Laurent Sabater, Christelle Hureau, Wojciech Bal
Summary: This study investigated the effect of Cu-II on the aggregation of A beta(1-40) and A beta(4-40). It was found that substoichiometric Cu-II concentrations accelerated aggregation, while superstoichiometric Cu-II inhibited fibril formation. The interaction between Cu-II, A beta(4-40), and A beta(1-40) resulted in significant changes in the aggregation process.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Qiong-Qiong Yao, Jitao Wen, Sarah Perrett, Si Wu
Summary: This study reveals the mechanisms by which lipid membranes influence the amyloid formation of Tau protein. It shows that lipid membranes can induce conformational changes in Tau and either accelerate or inhibit its fibrillation process depending on the concentration of the lipid membrane.
Article
Biochemistry & Molecular Biology
Martina Banchelli, Roberta Cascella, Cristiano D'Andrea, Giovanni La Penna, Mai Suan Li, Fabrizio Machetti, Paolo Matteini, Silvia Pizzanelli
Summary: Understanding the structural models of toxic species in Alzheimer's disease development is crucial for identifying early biomarkers and molecular mechanisms. Soluble oligomers of amyloid-beta peptides, particularly Aβ(1-42), are responsible for rapid impairment of brain functions by spreading cell damages. The study combining electron spin resonance spectroscopy and computational models on Aβ(1-42) samples provides structural constraints and suggests the Aβ(1-42) dimer as the building block of soluble oligomers, impacting the kinetic model of Aβ(1-42) aggregation.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Aleksandr Barulin, Prithu Roy, Jean-Benoit Claude, Jerome Wenger
Summary: The authors introduce optical horn antennas, a nanophotonic platform, for the label-free detection of UV autofluorescence from single proteins. This technique allows for real-time monitoring of protein unfolding and dissociation.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Vsevolod Borovkov
Summary: A novel experimental approach is proposed to study spin-correlated polaron pairs on conjugated polymer molecules, with the migration, recombination, and evolution of the spin state of the pairs monitored at nanosecond resolution by observing recombination fluorescence under different magnetic fields.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Review
Medicine, Research & Experimental
Hao Yu, Jie Wu
Summary: Amyloid-beta accumulation is a key feature of Alzheimer's disease, with both neurotoxic and potentially protective effects. Understanding the role of Aβ in depth will provide a broader perspective on Alzheimer's disease treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Chemistry, Multidisciplinary
Kai-Li Xin, Zheng-Li Hu, Shao-Chuang Liu, Xin-Yi Li, Jun-Ge Li, Hongyan Niu, Yi-Lun Ying, Yi-Tao Long
Summary: This study uses nanopore technology to simultaneously identify mutant and wild-type Aβ peptides through a multi-dimensional blockage mapping strategy. The standard deviation of current blockade fluctuations is proposed as a new supplement to accurately classify different blockage events. This work provides a new approach for the analysis of amyloid-beta peptide mutants.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Nir Salinas, Einav Tayeb-Fligelman, Massimo D. Sammito, Daniel Bloch, Raz Jelinek, Dror Noy, Isabel Uson, Meytal Landau
Summary: The study found that two subtypes of antimicrobial peptide uperin 3.5 can form functional amyloid fibrils, which has important physiological implications. Uperin 3.5 mainly forms cross-beta fibrils in the absence of lipids, but helical fibril formation is induced by bacterial cells or membrane mimetics, leading to membrane damage and cell death.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Alessandra Monti, Chiara Bruckmann, Francesco Blasi, Menotti Ruvo, Luigi Vitagliano, Nunzianna Doti
Summary: Peptides based on PREP1 can form amyloid-like aggregates with unique spectral properties under physiological conditions, exhibiting pH-dependent and reversible fluorescence during the structural transition from beta-sheet rich aggregates to alpha-helix structures. These findings demonstrate that the non-canonical fluorescence exhibited by amyloids is a complex phenomenon.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Dominik Goebel, Sandra Miguez-Lago, Maria Jose Ruedas-Rama, Angel Orte, Araceli G. Campana, Michal Juricek
Summary: By synthesizing a [6]helicene with ESIPT structural units, we have successfully combined CPL and ESIPT features into a single molecule. Our study shows that this compound exhibits strong CPL response and significant red-shifted emission, making it a promising candidate for applications in optoelectronics.
HELVETICA CHIMICA ACTA
(2022)
Meeting Abstract
Dermatology
Joseph F. Merola, Albert S. Chiou, Emmanuel During, Antonio Costanzo, Peter Foley, Marius Ardeleanu, Jiangming Wu, Zafer E. Ozturk
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Meeting Abstract
Dermatology
Joseph F. Merola, Albert S. Chiou, Emmanuel During, Antonio Costanzo, Peter Foley, Marius Ardeleanu, Jiangming Wu, Zafer E. Ozturk
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Letter
Dermatology
Michael L. Chen, Veronica Rotemberg, Jenna C. Lester, Roberto A. Novoa, Albert S. Chiou, Roxana Daneshjou
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Irene Lopez-Sicilia, Ana M. Ortuno, Pablo Reine, Dario Otero, Maria T. Martin-Romero, Luis Camacho, Luis Alvarez de Cienfuegos, Angel Orte, Juan J. Giner-Casares, Delia Miguel, Juan M. Cuerva
Summary: We describe the preparation and characterization of 2D films of (S,S,P)-1 and (R,R,M)-1ortho-oligophenylene ethylene (o-OPE) enantiomers, which exhibit high values of circularly polarized luminescence (CPL). These films are formed through precise 2D self-assembly at the air/water interface and transferred onto a solid glass support. The chiroptical properties of the transferred films increase with the number of layers, and CPL measurements show values similar to those in solution and independent of monomer concentration. A chiroptical barcoding system based on these films has been developed, demonstrating positive and negative regions.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Multidisciplinary Sciences
Alyssa Miller, Jiapeng Wei, Sarah Meehan, Christopher M. Dobson, Mark E. Welland, David Klenerman, Michele Vendruscolo, Francesco Simone Ruggeri, Tuomas P. J. Knowles
Summary: Neurodegenerative diseases such as Alzheimer's disease are caused by protein misfolding and aggregation into amyloid fibrils. This study uses atomic force microscopy and statistical theory to characterize amyloid ring structures derived from the brains of AD patients and explains the diversity in the structures formed from protein aggregation. The results show that ex vivo protofibril chains possess greater flexibility than mature amyloid fibrils, allowing them to form end-to-end connections and shedding light on their role in disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Dermatology
Kailas Vodrahalli, Justin Ko, Albert S. S. Chiou, Roberto Novoa, Abubakar Abid, Michelle Phung, Kiana Yekrang, Paige Petrone, James Zou, Roxana Daneshjou
Summary: Telemedicine use increased significantly during the COVID-19 pandemic, with skin conditions being a common use case. However, a large number of images submitted may be of insufficient quality for clinical determination. An AI decision support tool, utilizing a machine learning algorithm, was developed to provide real-time feedback and improve the quality of telemedicine images.
Article
Chemistry, Physical
M. Carmen Gonzalez-Garcia, Emilio Garcia-Fernandez, Jose L. Hueso, Pedro M. R. Paulo, Angel Orte
Summary: This work presents a method for controllably creating silver micropatterns by laser-induced photosculpting. The process involves plasmonic interactions between pulsed laser radiation and silver nanorods, leading to the formation of well-defined 3D structures known as Airy castles. The structures contain emissive Ag nanoclusters, allowing for the examination of the aggregation process using luminescence microscopy. The study comprehensively examines the factors that define the photosculpting process and investigates the potential applications of Airy castles.
Article
Dermatology
Michelle Phung, Vijaytha Muralidharan, Veronica Rotemberg, Roberto Andres Novoa, Albert Sean Chiou, Christoph Y. Sadee, Bailie Rapaport, Kiana Yekrang, Jared Bitz, Olivier Gevaert, Justin Meng Ko, Roxana Daneshjou
Summary: Recent developments in artificial intelligence research have led to the increased use of algorithms for detecting malignancies in clinical and dermoscopic images of skin diseases. Gathering training and testing data is crucial for these methods. This paper explores the best practices and challenges in collecting skin images and data for translational artificial intelligence research, including ethics, image acquisition, labeling, curation, and storage. The aim is to enhance malignancy detection using artificial intelligence by facilitating intentional data collection and collaboration between dermatologists and data scientists.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Correction
Biochemical Research Methods
John S. H. Danial, Jeff Y. L. Lam, Yunzhao Wu, Matthew Woolley, Eleni Dimou, Matthew R. Cheetham, Derya Emin, David Klenerman
Article
Clinical Neurology
Shyam K. Akula, Allen Y. Chen, Jennifer E. Neil, Diane D. Shao, Alisa Mo, Norma K. Hylton, Stephanie DiTroia, Vijay S. Ganesh, Richard S. Smith, Katherine O'Kane, Rebecca C. Yeh, Jack H. Marciano, Samantha Kirkham, Connor J. Kenny, Janet H. T. Song, Muna Al Saffar, Francisca Millan, David J. Harris, Andrea V. Murphy, Kara C. Klemp, Stephen R. Braddock, Harrison Brand, Isaac Wong, Michael E. Talkowski, Anne O'Donnell-Luria, Abbe Lai, Robert Sean Hill, Ganeshwaran H. Mochida, Ryan N. Doan, A. James Barkovich, Edward Yang, Dina Amrom, Eva Andermann, Annapurna Poduri, Christopher A. Walsh, Polymicrogyria Genet Res Network
Summary: Polymicrogyria is a common cortical malformation associated with various neurological impairments. This genetic association study identified several known and novel genes associated with polymicrogyria, suggesting a higher rate of identifiable genetic causes in affected individuals.
Article
Chemistry, Multidisciplinary
Alvaro Ruiz-Arias, Francisco Fueyo-Gonzalez, Carolina Izquierdo-Garcia, Amparo Navarro, Marta Gutierrez-Rodriguez, Rosario Herranz, Chiara Burgio, Antonio Reinoso, Juan M. Cuerva, Angel Orte, Juan A. Gonzalez-Vera
Summary: Lanthanides have unique photoluminescence properties and are ideal for biological imaging applications. However, photobleaching is a major drawback of photoluminescence imaging microscopy. In this study, a small aqueous-soluble lanthanide antenna was developed to mitigate photobleaching in PLIM microscopy, allowing for more stable imaging.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Clinical Neurology
Shyam K. Akula, Allen Y. Chen, Jennifer E. Neil, Diane D. Shao, Alisa Mo, Norma K. Hylton, Stephanie DiTroia, Vijay S. Ganesh, Richard S. Smith, Katherine O'Kane, Rebecca C. Yeh, Jack H. Marciano, Samantha Kirkham, Connor J. Kenny, Janet H. T. Song, Muna Al Saffar, Francisca Millan, David J. Harris, Andrea V. Murphy, Kara C. Klemp, Stephen R. Braddock, Harrison Brand, Isaac Wong, Michael E. Talkowski, Anne O'Donnell-Luria, Abbe Lai, Robert Sean Hill, Ganeshwaran H. Mochida, Ryan N. Doan, A. James Barkovich, Edward Yang, Dina Amrom, Eva Andermann, Annapurna Poduri, Christopher A. Walsh
Summary: This study identified genetic variants associated with polymicrogyria through genetic sequencing, expanding the understanding of the genetic causes of this condition.
Article
Chemistry, Multidisciplinary
Alvaro Ruiz-Arias, Rocio Jurado, Francisco Fueyo-Gonzalez, Rosario Herranz, Natividad Galvez, Juan A. Gonzalez-Vera, Angel Orte
Summary: In this article, a methodology for imaging different stages of amyloid aggregation using a pair of dyes capable of binding aggregates and undergoing energy transfer (FRET) is presented. The optimization process for choosing the best dye pair is described, involving a screening of different naphthalimides, quinolimides, and amyloid-binding dyes. This research is important for a better understanding of protein aggregation processes.
RESULTS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Antonio Fernandez, Nicola Kielland, Ashraff Makda, Neil O. Carragher, M. Carmen Gonzalez-Garcia, Laura Espinar-Barranco, Juan A. Gonzalez-Vera, Angel Orte, Rodolfo Lavilla, Marc Vendrell
Summary: We present a platform that combines multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. Through optimization, we identified compound NIRBD-62c as a multimodal probe suitable for STED super-resolution and fluorescence lifetime imaging. Additionally, we used NIRBD-62c to image intracellular trafficking and examine the effects of pharmacological inhibitors on vesicular traffic between intracellular compartments and the plasma membrane.
RSC CHEMICAL BIOLOGY
(2022)