Article
Infectious Diseases
Ayse Oen, Djenana Vejzovic, James Jennings, Lena Parigger, Robert A. Cordfunke, Jan Wouter Drijfhout, Karl Lohner, Nermina Malanovic
Summary: OP-145 and SAAP-148, derived from LL-37, show effective killing against both Gram-positive and Gram-negative bacteria. However, their impact on Escherichia coli does not align with membrane permeabilization. In this study, we used a combination of techniques to investigate their mode of action and found distinct binding patterns and permeabilization effects depending on the LPS phenotypes. Our findings suggest that depolarization of the cytoplasmic membrane and disruption of lipid packing are crucial for their bactericidal activity.
Article
Chemistry, Medicinal
Takumi Kaji, Yoshiaki Yano, Katsumi Matsuzaki
Summary: Antimicrobial peptides may exert bactericidal activity by disrupting the lipid matrix of bacterial membranes, but the actual number of peptide molecules bound to bacterial cells exceeds that expected from liposomal studies, raising questions about the relevance of membrane permeabilization mechanisms proposed by such studies.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Physical
Arpita Roy, Nirod Kumar Sarangi, Surajit Ghosh, Amrutha Prabhakaran, Tia E. Keyes
Summary: Antimicrobial peptides (AMPs) have significant potential in combating antibiotic resistance by targeting microbial membrane without harming mammalian cells. This study investigated the interactions and synergistic effects of two AMPs, magainin 2 and PGLa, on bacterial and mammalian membrane models. The combined AMPs formed toroidal pores, while individual AMPs only affected the exterior leaflet of the bacterial model. The impact on a ternary, mammalian mimetic membrane was much weaker.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Medicinal
Aliasghar Sepehri, Binod Nepal, Themis Lazaridis
Summary: Research shows that the structure and stability of pores formed by islet amyloid polypeptide in cell membranes are related to its configuration and helical structure, which may result in inconsistent results observed in different experiments.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Microbiology
Laurence Fermon, Agnes Burel, Emeline Ostyn, Stephane Dreano, Arnaud Bondon, Soizic Chevance, Marie-Laure Pinel-Marie
Summary: This study investigates the toxicity and mechanism of action of two peptides encoded by the sprG1 gene in the SprG1/SprF1 toxin-antitoxin system of Staphylococcus aureus prophage. The results show that both peptides can inhibit bacterial growth, with higher toxicity observed for SprG131. The combination of a hydrophobic domain and a charged domain at the C-terminus is necessary for this toxicity. Furthermore, the study reveals the chronology of toxic events, including the formation of mesosome-like structures, membrane depolarization, and depletion of intracellular ATP leading to growth arrest.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Liangbo Hu, Ying Li, Xinhui Lin, Yucheng Huo, Hongyue Zhang, Huaimin Wang
Summary: By designing, synthesizing, and assembling an enzymatic programmable peptide system, we induced molecular assemblies formation spatiotemporally in living cancer cells, resulting in mainly necroptosis of glioblastoma cells. The stability and glycosylation of molecules play a crucial role in determining the bioactivity, as evidenced by detailed mechanistic studies showing site-specific formation of assemblies capable of activating the downstream cell death pathway.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Liangbo Hu, Ying Li, Xinhui Lin, Yucheng Huo, Hongyue Zhang, Huaimin Wang
Summary: The research article reports a structure-based programming approach of supramolecular assemblies in living cells for selective cancer cell inhibition by Wang et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
William T. Julian, Anastasia V. Vasilchenko, Daniil D. Shpindyuk, Darya V. Poshvina, Alexey S. Vasilchenko
Summary: The antibacterial mechanism of 2,4-DAPG involves an increase in membrane permeability and a reduction in bacterial viability, with different effects observed in E. coli and S. aureus. In addition, 2,4-DAPG inhibits the metabolic activity of Staphylococcus aureus and Escherichia coli in mature biofilms.
Article
Multidisciplinary Sciences
Maya Sen, Dylan Mooijman, Alex Chialastri, Jean-Charles Boisset, Mina Popovic, Bjorn Heindryckx, Susana M. Chuva de Sousa Lopes, Siddharth S. Dey, Alexander van Oudenaarden
Summary: Erasure of DNA methylation from the parental genomes is crucial for resetting the methylome of differentiated gametes to pluripotent cells in the blastocyst. Researchers present a high-throughput single-cell method enabling strand-specific quantification of DNA methylation and identify distinct modes of DNA demethylation dynamics during early mammalian development.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Carolina dos Anjos, Leon G. Leanse, Martha S. Ribeiro, Fabio P. Sellera, Milena Dropa, Victor E. Arana-Chavez, Nilton Lincopan, Mauricio S. Baptista, Fabio C. Pogliani, Tianhong Dai, Caetano P. Sabino
Summary: Antimicrobial drug development is under scrutiny due to the worldwide antibiotic crisis. Antimicrobial blue light (aBL) has been recognized as a potential therapy for infections, but the specific targets for bacterial killing by aBL are still poorly understood and may vary depending on the bacterial species.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Janine H. Peterson, Matthew Thomas Doyle, Harris D. Bernstein
Summary: Several antibacterial compounds have been found to inhibit BAM function through different mechanisms, affecting the biogenesis of outer membrane proteins in Gram-negative bacteria. These findings not only provide important insights for developing novel broad-spectrum antibiotics, but also valuable tools for future studies on BAM function.
Article
Multidisciplinary Sciences
N. Frazao, A. Konrad, M. Amicone, E. Seixas, D. Guleresi, M. Laessig, I Gordo
Summary: By monitoring evolution for more than six thousand generations in the mouse gut, the authors show that a colonizing bacterial strain evolves through two modes: one involving metabolic mutations and the other involving the domestication of bacteriophages. The study demonstrates the importance of the microbiota diversity and the presence of related strains in the colonization success of Escherichia coli. The research also highlights the rapid formation of ecotypes and phage domestication in the mammalian gut.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Tim Axelrod, Evgeni Eltzov, Merav Lerman, Dorin Harpaz, Robert S. Marks
Summary: This study demonstrates that cigarette filters can reduce the harmful effects of cigarette smoke, but they cannot completely eliminate the impact on the human body. Smoking also affects quorum sensing and increases the risk of pulmonary infections.
Review
Food Science & Technology
Sylvie Nonin-Lecomte, Laurence Fermon, Brice Felden, Marie-Laure Pinel-Marie
Summary: Bacterial type I toxin-antitoxin systems consist of stable toxic proteins and unstable RNA antitoxins, regulating growth arrest or cell death. These systems, with small hydrophobic peptides, impact intracellular ATP levels and play roles in plasmid maintenance, environmental stress adaptation, or persister cell formation.
Article
Multidisciplinary Sciences
Geoffrey N. Gobert, Donald P. McManus, Geoff McMullan, Christopher J. Creevey, Jack Carson, Malcolm K. Jones, Sujeevi S. K. Nawaratna, Kosala G. Weerakoon, Hong You
Summary: This study used FISH and microbial profiling techniques to demonstrate for the first time the presence of bacteria on the epithelial surfaces of adult schistosomes, which differ from the host blood and bile. These schistosome surface-associated bacteria are considered an important component of the host-parasite interaction.
Article
Microbiology
Fuminori Yoneyama, Yuichi Imura, Kanako Ohno, Takeshi Zendo, Jiro Nakayama, Katsumi Matsuzaki, Kenji Sonomoto
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2009)
Article
Microbiology
Fuminori Yoneyama, Kanako Ohno, Yuichi Imura, Mengqi Li, Takeshi Zendo, Jiro Nakayama, Katsumi Matsuzaki, Kenji Sonomoto
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2011)
Article
Biotechnology & Applied Microbiology
Fuminori Yoneyama, Yuichi Imura, Shiro Ichimasa, Koji Fujita, Takeshi Zendo, Jiro Nakayama, Katsumi Matsuzaki, Kenji Sonomoto
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2009)
Article
Multidisciplinary Sciences
Yuichi Imura, Toshiaki Tagawa, Yuya Miyamoto, Satoshi Nonoyama, Hiroshi Sumichika, Yasuhiro Fujino, Masaya Yamanouchi, Hideo Miki
Summary: The study found that adding an alkaline wash step in protein A purification can improve the physicochemical properties of monoclonal antibodies.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Minoru Nishida, Yuichi Imura, Megumi Yamamoto, Satoe Kobayashi, Yoshiaki Yano, Katsumi Matsuzaki
Article
Biochemistry & Molecular Biology
Yuichi Imura, Minoru Nishida, Katsumi Matsuzaki
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2007)
Article
Biochemistry & Molecular Biology
Yuichi Imura, Minoru Nishida, Yoshiyuki Ogawa, Yoshinobu Takakura, Katsumi Matsuzaki
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2007)
Article
Biochemistry & Molecular Biology
S Kobayashi, A Chikushi, S Tougu, Y Imura, M Nishida, Y Yano, K Matsuzaki