Article
Chemistry, Multidisciplinary
Hongguo Wei, Shengjun Bu, Ze Wang, Hongyu Zhou, Xue Li, Jiaqi Wei, Xiuxia He, Jiayu Wan
Summary: This study introduced CRISPR-Cas12a and click chemistry-mediated exponential amplification reaction into an electrochemical biosensor for sensitive detection of miRNA-21. The proposed biosensor showed a low detection limit and has potential applications in point-of-care testing and field molecular diagnostics.
Article
Chemistry, Analytical
Shiyi Wang, Ziwen Zong, Jianguo Xu, Bangben Yao, Zhou Xu, Li Yao, Wei Chen
Summary: In this study, a visual strategy for rapid and ultrasensitive detection of OTA was proposed by integrating primer-mediated exponential rolling circle amplification (P-ERCA) with a designed nucleic acid lateral flow strip (LFS). This method demonstrates highly sensitive detection of OTA and could be utilized as a universal method for detection of other chemical analytes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Hermann Neitz, Claudia Hoebartner
Summary: We report the application of a fluorescent nucleoside called ToldU as a photoresponsive crosslinker in three different DNA architectures, resulting in enhanced fluorescence emission. The fluorogenic ToldU crosslinking reaction allows for the assembly of DNA polymers through a hybridization chain reaction, enabling the concentration-dependent detection of a specific DNA sequence. A new photoinduced fluorogenic interstrand crosslinking reaction is demonstrated in three different DNA architectures using the hydrophobic tolane-containing nucleoside, ToldU.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biophysics
Yan Shan Ang, Xinzhi Qiu, Hong Meng Yam, Ningjing Wu, Lin-Yue Lanry Yung
Summary: This study demonstrates the use of a split proximity circuit (SPC), a plug-and-play molecular detection platform, which achieves a selectivity comparable to qRT-PCR for quick and easy miRNA quantification.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Shah Zada, Huiting Lu, Wenhao Dai, Songsong Tang, Sikandar Khan, Fan Yang, Yuchun Qiao, Pengcheng Fu, Haifeng Dong, Xueji Zhang
Summary: The study developed an amplified imaging platform for intracellular miRNAs based on Mo2B nanosheets, which combined fluorescence quenching and hybridization chain reaction technologies for sensitive detection and imaging of miRNAs. This platform demonstrated excellent performance in distinguishing cancer cells from normal cells and monitoring miRNA expression changes in cancer cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Motoi Oishi, Shotaro Juji
Summary: Accelerated DNA hybridization chain reactions (HCRs) using DNA origami as a scaffold have attracted attention in dynamic DNA nanotechnology. In this study, semilocalized and localized HCR systems were constructed using magnetic beads (MBs) as a simple scaffold for enzyme-free miR-21 detection. The DNA density on MBs was found to be crucial for HCR kinetics and limit of detection.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Medicinal
Zhen Song, Qiang-Yan Zhang, Jia-Jing Li, Jing-Lian Su, Yuan-Hua Liu, Gong-Jun Yang, Huai-Song Wang
Summary: Let-7a, a type of low-expressed microRNAs in cancer cells, has been investigated as a promising biomarker and therapeutic target for tumor suppression. Developing simple and sensitive detection methods for let-7a is important for cancer diagnosis and treatment. In this work, the hybridization chain reaction (HCR) was used to initiate the detection of let-7a, followed by analysis using lateral flow assay (LFA) and electrochemical impedance spectroscopy.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Liuting Mo, Danlian Liang, Mingxiu Mo, Chan Yang, Weiying Lin
Summary: In this study, a 3D hybridization chain reaction (HCR) on a binary DNA tetrahedron (DTN) system was used for multiplexed detection of miR-21 and miR-203, enabling accurate, sensitive, and rapid analysis of intracellular miRNA levels.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
LianLian Xu, Weihua Zhao, Jiamei Pu, Suqin Wang, Shiwen Liu, Hongbo Li, Ruqin Yu
Summary: In this study, a biosensor based on Lambda exonuclease and HCR enzyme-free amplification system was designed to detect Pax-5a gene with high sensitivity. The biosensor showed advantages of simple operation, high sensitivity, and good selectivity, and has potential applications in the early diagnosis of leukemia and biosensor design.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Liang-Yong Yang, Xiong-Wei Xu, Yan Lin, Chen-Liu Ye, Wei-Qiang Liu, Zhou-Jie Liu, Guang-Xian Zhong, Yan-Fang Xu, Xin-Hua Lin, Jin-Yuan Chen
Summary: This study developed a nucleic acid template-dominated click chemistry-mediated ligation chain reaction (cc-eLCR) for efficient and specific nucleic acid amplification. The cc-eLCR method, which utilized nM-level DNA probes and an electrochemical platform, successfully detected low-level nucleic acid targets with single-base resolution. Moreover, the cc-eLCR assay was also applied to detect target molecules in cell extracts, showing its potential in medical diagnosis and biomedical research.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jieyu Hu, Xingxing Yu, Xiumei Zhuang, Yan Sun, Junjie Wang, Haiting Ren, Shenghao Zhang, Yueshou Zhang, Hongxing Qiu, Yongjun Hu
Summary: We report a novel SERS tandem strategy with high sensitivity for detecting the p53 gene. By utilizing copper oxide nanoparticles to catalyze the click reaction between azide and alkyne, and amplifying the T-DNA signal through hybridization chain reaction, we successfully obtained highly sensitive and selective SERS signals in complex biological matrices. The constructed sensor has excellent clinical application prospects.
ANALYTICA CHIMICA ACTA
(2022)
Article
Nanoscience & Nanotechnology
Selma Piranej, Alisina Bazrafshan, Khalid Salaita
Summary: The authors introduce a new paradigm for DNA computation using dynamic DNA-based motors to transduce chemical inputs into mechanical outputs in the form of macroscopic locomotion. This method shows fast computation speeds and easy electronic detection.
NATURE NANOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Huo Xu, Min Lin, Yanhui Zheng, Xiaojun Fang, Xinmei Huang, Qi Huang, Jiawei Xu, Wei Duan, Juan Wei, Lee Jia
Summary: This study designed a multifunctional linear DNA nanostructure (MLN) as a carrier of molecular beacons (MB-21) for detecting and intracellular imaging miRNA-21. The MLN-MB exhibited a detection limit of 320 pM for miRNA-21 and high specificity against other miRNAs. With its outstanding stability, dual recognition, and biocompatibility, MLN-MB holds great potential for early cancer diagnosis.
Article
Chemistry, Analytical
Juan Chen, Mingyue Xie, Mingqing Shi, Kun Yuan, Yanan Wu, Hong-Min Meng, Lingbo Qu, Zhaohui Li
Summary: In this study, an accelerated DNA nanoprobe was developed for fast, in situ monitoring of miRNA in exosomes. The nanoprobe not only detects miRNA, but also distinguishes tumor exosomes from those derived from normal cells, and can be used to study exosome biological functions.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Nana Kang, Benrui Weng, Sijia Liu, Huiran Yang, Siyuan Wang, Yaqi Liu, Jiabing Ran, Hanghang Liu, Zhangshuang Deng, Changying Yang, Huimin Wang, Fuan Wang
Summary: The study presents a multistage chemiluminescence resonance energy transfer (CRET)-based DNA circuit for accurate miRNA detection and cell imaging. The circuit utilizes catalytic hairpin assembly (CHA), hybridization chain reaction (HCR), and DNAzyme to regulate the distance between the donor and acceptor, resulting in amplified luminescence and reactive oxygen species (ROS) signals. The circuit achieves high sensitivity and specificity through multiple recognition of the target and programmable DNA nanostructures, making it a promising tool for early diagnosis and theranostics.