Article
Chemistry, Analytical
Han Zhao, Xinfa Liu, Changbei Ma
Summary: The study presents a novel fluorometric strategy based on the Cu2+-TH system for the detection of ALP activity and inhibition, demonstrating remarkable sensitivity and a linear detection range. The method was successfully applied to human serum samples, showing its reliability for clinical diagnosis of ALP-related diseases.
Article
Biochemical Research Methods
Wenbin Wang, Jing Lu, Lulu Hao, Huaixia Yang, Xuejie Song, Fuchun Si
Summary: A novel electrochemical method utilizing aminoferrocene as an electroactive probe for alkaline phosphatase (ALP) activity detection was described in this study. The approach demonstrated good linear relationship and sensitivity in detecting ALP activity, with high selectivity and applicability.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Shengnan Fan, Xingxing Jiang, Minghui Yang, Xianggui Wang
Summary: Cu@PB NCs exhibit enhanced POD-like activity compared to PB NCs and HRP, and can be utilized for colorimetric assay of ALP activity by oxidizing TMB in the presence of H2O2. Introduction of PPi results in decreased POD-like activity of Cu@PB NCs, while ALP hydrolyzes PPi and restores the activity of Cu@PB NCs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Brandon S. McCullough, Hanfei Wang, Amy M. Barrios
Summary: Little is known about the biological roles of the protein histidine phosphatase PHPT1, and the lack of chemical tools for studying its phosphorylation and dephosphorylation has hindered progress in the field. Through an inhibitor screen using a fluorescent assay, norstictic acid was identified as a potent inhibitor of PHPT1 activity.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Review
Chemistry, Inorganic & Nuclear
Samy M. Shaban, Sae Byeok Jo, Eslam Hafez, Jeong Ho Cho, Dong-Hwan Kim
Summary: This review provides a comprehensive overview of recent advances in ALP assays and immunoassay strategies. It discusses the different techniques and their comparison, offering a convenient route for developing more advanced ALP sensors.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Analytical
Zhengxian Lv, Qiuquan Wang, Minghui Yang
Summary: A sensing platform was developed for determining alkaline phosphatase activity based on the cooperation of DNAzyme-Au spherical nucleic acid nanoprobe with the graphene-oxide-loaded hybridization chain reaction system. The assay utilizes the strong affinity between pyrophosphate and Cu2+ ions, as well as the hydrolysis of pyrophosphate by ALP to release Cu2+ ions, to achieve good detection sensitivity and specificity. The feasibility of the method was validated by spiking ALP standards in bovine serum, showing potential for drug screening applications.
Article
Oncology
Michinari Okamoto, Shigeru Yamaguchi, Yukitomo Ishi, Hiroaki Motegi, Takashi Mori, Takayuki Hashimoto, Yukayo Terashita, Shinsuke Hirabayashi, Minako Sugiyama, Akihiro Iguchi, Yuko Cho, Atsushi Manabe, Kiyohiro Houkin
Summary: The CSF-PLAP value is a useful biomarker for the initial diagnosis and recurrence of IGCTs, and may be associated with the volume of germinomatous components of tumors.
Article
Chemistry, Analytical
Yolanda Alacid, Rocio Esquembre, Francisco Montilla, Maria Jose Martinez-Tome, C. Reyes Mateo
Summary: This work presents the development and characterization of fluorescent nanocomposite hydrogels using a green protocol. The hydrogels have high swelling and absorption capacity and are prepared by incorporating polyfluorenes-based nanoparticles into a three-dimensional polymeric network. The hydrogels show good storage stability and homogeneity when using the ex situ method. They have been tested as a sensing platform for the detection of alkaline phosphatase with a quick response time and low LOD, making them promising for quantifying physiological ALP levels and enzyme activity in environmental samples.
Article
Chemistry, Multidisciplinary
Yujie Sun, Lei Wen, Huili Ma, Wenlin Ma, Zhenqian Fu, Yinhui Li, Chengwu Zhang, Lin Li, Jinhua Liu
Summary: In this study, a trienzyme cascade-triggered fluorescent immunosensor platform was constructed by integrating alkaline phosphatase (ALP), tyrosinase (TYR), and horseradish peroxidase (HRP). The platform utilized a rapid in situ fluorogenic reaction to produce a yellow fluorescent compound. The developed immunosensor showed significant enhancements in fluorescence and successfully detected a cardiac biomarker in clinical samples.
CHINESE CHEMICAL LETTERS
(2023)
Article
Spectroscopy
Yu Ding, Tianxia Lin, Jiwei Shen, Yinmao Wei, Chaozhan Wang
Summary: In this study, a reaction-based ratiometric fluorescence assay was proposed for the detection of alkaline phosphatase (ALP). The method demonstrated high sensitivity, good selectivity, cost efficiency, and easy operation.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Chemistry, Analytical
Yusuke Kanno, Yuanshu Zhou, Takeshi Fukuma, Yasufumi Takahashi
Summary: This review summarizes the recent research progress of ALP-based electrochemical devices for point-of-care testing, discussing the applications of ALP substrates, immunosensors, aptasensors, and DNAzyme sensors.
Review
Chemistry, Multidisciplinary
Meng Li, Bhaskar Gurram, Shan Lei, Nicholas T. Blum, Peng Huang, Jing Lin
Summary: Phosphatase is crucial in many biological functions, with alkaline phosphatase (ALP) serving as a key prognostic indicator for various diseases, though current detection methods are limited to in vitro colorimetric techniques. Fluorescence imaging provides a reliable way to monitor real-time changes in ALP levels, with research focusing mainly on ALP imaging probes grouped by their luminescence mechanisms. Challenges and future prospects for phosphatase probes were also discussed.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Yu-Ting Huang, Ke-Xin Xu, Xing-Shi Liu, Zheng Li, Jin Hu, Ling Zhang, Yuan-Cheng Zhu, Wei-Wei Zhao, Hong-Yuan Chen, Jing-Juan Xu
Summary: This work combines chemical amplification with electronic amplification in the organic photoelectrochemical transistor (OPECT), achieving dual-amplified bioanalysis and sensitive detection of human immunoglobulin G. The study is significant for OPECT bioanalysis.
ANALYTICAL CHEMISTRY
(2023)
Review
Oncology
Chengying Jiang, Fangke Hu, Jiazhen Li, Guangshen Gao, Xiaojing Guo
Summary: The purpose of this meta-analysis was to summarize the diagnostic value of serum alkaline phosphatase (ALP) and bone-specific isoforms (BAP) in metastatic breast cancer (BC), with a focus on bone metastases. High levels of ALP and BAP in serum were found to be associated with a higher detection rate of bone metastatic BC patients. Although not highly reliable, serum ALP and BAP still provide some useful information for early detection of BC metastases, especially bone metastases.
BREAST CANCER RESEARCH AND TREATMENT
(2023)
Article
Chemistry, Multidisciplinary
Xianqing Tang, Jinghuang Chen, Mengqian Zhang, Jian Sun, Xiurong Yang
Summary: A facile hydrothermal method was developed to prepare FeWO4 nanomaterials with intrinsic peroxidase-like activity, and the catalytic activity was significantly enhanced by adjusting the size and oxygen vacancy content. A colorimetric assay based on FeWO4 was constructed to detect PPi and ALP activity. The proposed method successfully evaluated ALP activity in real serum samples and screened for potential ALP inhibitors.
SCIENCE CHINA-CHEMISTRY
(2023)
