Article
Biochemical Research Methods
Kazi Morshed Alom, Young Jun Seo
Summary: This study developed a method combining a rolling circle transcription (RCT) system and a QnMorpholine (QNM) fluorescent probe for selective and sensitive detection of alkaline phosphatase (ALP). By producing a large amount of RCT 22AG G-quadruplex in the absence of ALP, a high fluorescence signal was generated, while no G-quadruplex was produced in the presence of ALP. This significant difference in signal intensity allowed for the identification of ALP presence or absence in a test sample, even at extremely low concentrations. The simplicity and efficiency of this approach suggest its potential as a reliable diagnostic tool for ALP detection.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Yunfeng Bai, Huilin Zhang, Lu Zhao, Yuzhen Wang, Xiaoliang Chen, Hong Zhai, Maozhong Tian, Ruirui Zhao, Tao Wang, Hui Xu, Feng Feng
Summary: This study developed a rationally designed aptasensing platform based on HCR and G-quadruplex DNAzyme for the fluorescence detection of CEA. The platform showed high selectivity for CEA and was successfully applied in diluted human serum samples, demonstrating promising potential in biochemical analysis and clinic application.
Article
Biochemistry & Molecular Biology
Dimitrios Diamantis, Antonios D. Tsiailanis, Christina Papaemmanouil, Maria-Christina Nika, Zoi Kanaki, Simona Golic Grdadolnik, Andrej Babic, Eleftherios Paraskevas Tzakos, Isabelle Fournier, Michel Salzet, Prem Prakash Kushwaha, Nikolaos S. Thomaidis, Theodoros Rampias, Eswar Shankar, Serdar Karakurt, Sanjay Gupta, Andreas G. Tzakos
Summary: A novel prodrug approach was developed to inhibit cancer progression by targeting alkaline phosphatase (ALP) in the tumor microenvironment (TME). The prodrug, phospho-apigenin, increased the stability of apigenin and showed enhanced antiproliferative effect in malignant cells with elevated ALP levels compared to apigenin alone. In vivo experiments also demonstrated its significant tumor growth suppression.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Spectroscopy
Yan Wang, Zhenwei Tang, Xueling Shang, Ying Yan, Xinying Xiang, Changbei Ma
Summary: In this study, a novel method for alkaline phosphatase (ALP) analysis based on Pb2+ dependent DNAzyme was described. By modifying the DNAzyme sequence and introducing exonuclease I, ALP analysis was successfully achieved with reduced background noise. The method exhibited a low detection limit and a wide linear range, and showed excellent performance in practical applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Yufen Lai, Mengyan Li, Xiaofei Liao, Li Zou
Summary: In this study, a DNAzyme-regulated CRISPR/Cas12a cascade signal amplification strategy was developed for sensitive and selective detection of ALP activity and inhibition. The proposed method overcomes the shortcomings of conventional methods and shows great potential in clinical diagnosis and biomedical research.
ANALYTICA CHIMICA ACTA
(2022)
Article
Spectroscopy
Yao Chen, Jin Yan, Xiaozhi Wang, Siwei Zhang, Jun Li, Ying Tang, Tong Wang
Summary: A fluorescence sensing method was developed for the quantification of alkaline phosphatase (ALP) by integrating strand displacement amplification with DNAzyme-catalytic recycling cleavage of molecular beacons. The proposed method achieved sensitive and specific ALP detection in human serum samples, showing potential for application in biomedical research and clinical diagnosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Jingrui Cao, Qiong Wu, Xin Chang, Hongyu Chu, Hong Zhang, Xuedong Fang, Fangfang Chen
Summary: In this study, a small organic molecule-based ratiometric fluorescent probe was designed to effectively and sensitively detect the activity changes of alkaline phosphatase. The probe showed high selectivity and sensitivity under experimental conditions, and the measurement results in human serum samples matched well with those obtained using commercial kits. Additionally, the probe was successfully applied to image alkaline phosphatase in living hepatocytes with good selectivity and sensitivity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Yolanda Alacid, Andres F. Quintero Jaime, Maria Jose Martinez-Tome, C. Reyes Mateo, Francisco Montilla
Summary: This study describes the development of a portable electrochemical biosensor using alkaline phosphatase (ALP) as a recognition element and immobilizing it in acrylamide-based hydrogels. The biosensor was prepared through a green protocol and deposited on disposable screen-printed electrodes. The electrochemical transduction was carried out by using an electroinactive substrate and measuring the activity of the protein within the hydrogel matrix. The biosensor showed increased sensitivity due to the adhesive properties of the hydrogel and was optimized for the determination of phosphate ion in aqueous media. The study demonstrates the potential use of this biosensor for real-time measurement of water pollutants that inhibit ALP.
Article
Medicine, General & Internal
Hussein Abdullah Rajab
Summary: This study found that patients with 25-hydroxyvitamin D (25OHD) levels below 15 ng/mL were more likely to experience symptoms, and there was a correlation between vitamin D level and changes in parathyroid hormone (PTH) and alkaline phosphatase (ALP).
Article
Chemistry, Multidisciplinary
Lei Chen, Wenjing Qi, Chengpei Du, Yi Wang, Chun Liu, Xiaomei Huang, Xiaojuan Chang
Summary: A high-selectivity Cu2+ fluorescent probe was synthesized via a one-step Mannich reaction, which can effectively detect Cu2+ concentration and rapidly detect PPi and ALP.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Biophysics
Guojie Qin, Lixiang Zuo, Yanli Wei, Li Wang, Graham Bodwell
Summary: The paper introduces a highly sensitive sensing system for alkaline phosphatase using room temperature phosphorescence, with intense emission in the absence of alkaline phosphatase that gradually decreases upon addition of the enzyme. Factors affecting the performance of the sensing system were optimized, resulting in a linear range for alkaline phosphatase from 0.2-10 U/L with a LOD at 0.045 U/L and a recovery rate in human serum from 93.75%-103.03%. Additionally, an RTP-based INHIBIT logic gate utilizing doped ZnS quantum dots was presented.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Endocrinology & Metabolism
Kaori Soma, Masanori Izumi, Yuko Yamamoto, Shojiro Miyazaki, Kengo Watanabe
Summary: The study demonstrates that DS-1211 is an effective selective TNAP inhibitor with potential pharmaceutical characteristics to prevent ectopic soft tissue calcification. In vitro and in vivo experiments show that DS-1211 acts by inhibiting ALP activity and increasing PPi levels, exhibiting good pharmacokinetic properties and cross-species selective inhibition effects.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Chemistry, Physical
Yunqin Zhang, Yang Cao, Yan Liu, Yingjie Yang, Mingmao Chen, Hang Gao, Lushan Lin, Xueyuan Chen
Summary: Enzyme-responsive nanomaterials have great potential in the prognosis or diagnosis of disease biomarkers. However, there are various obstacles that hinder their practical application, including autofluorescence interference, poor monodispersity, uncontrollable size and morphology, low optical stability, and biotoxicity. In this study, a novel strategy using lanthanide nucleotide nanoparticles was proposed for reliable and precise detection of the enzyme disease biomarker alkaline phosphatase (ALP). The nanoparticles showed improved monodispersity and uniformity, and demonstrated superior selectivity and sensitivity for ALP detection in complicated serum samples.
Article
Chemistry, Multidisciplinary
Ping-Hsuan Hsieh, Cheng-Yan Yeh, Chang-Ming Wang, Wei-Ssu Liao, Chong-You Chen
Summary: This study introduces a colorimetric assay for alkaline phosphatase (ALP) detection using metal-free nanozymatic carbon dots. The assay offers a highly sensitive and selective method for measuring ALP activity with a low detection limit.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Chemistry, Analytical
Haiyan Li, Shangqing Zhang, Mingli Chen, Jianhua Wang
Summary: Bimetallic dual-ligands terbium nanocomplexes with unique internal structure were fabricated through in situ coordination assembly. By regulating the proportion of internal ligand SSA, the nanocomplexes showed excellent sensing specificity for detecting phosphate and pyrophosphate, with low detection limits.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)