4.8 Article

Controlling chitosan-based encapsulation for protein and vaccine delivery

期刊

BIOMATERIALS
卷 35, 期 14, 页码 4382-4389

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.01.078

关键词

Chitosan particles; Protein encapsulation; Vaccine delivery; Precipitation-coacervation; Microencapsulation; Nanoencapsulation

资金

  1. National Cancer Institute [K22 CA131567]
  2. National Institute of General Medical Sciences [P30 GM103450]
  3. National Center for Research Resources of the National Institutes of Health [P20 RR15569]
  4. Department of Energy [DE-FG02-01ER15161]
  5. National Science Foundation [IOS0843397 9TO]
  6. Arkansas Biosciences Institute

向作者/读者索取更多资源

Chitosan-based nano/microencapsulation is under increasing investigation for the delivery of drugs, biologics and vaccines. Despite widespread interest, the literature lacks a defined methodology to control chitosan particle size and drug/protein release kinetics. In this study, the effects of precipitation-coacervation formulation parameters on chitosan particle size, protein encapsulation efficiency and protein release were investigated. Chitosan particle sizes, which ranged from 300 nm to 3 mu m, were influenced by chitosan concentration, chitosan molecular weight and addition rate of precipitant salt. The composition of precipitant salt played a significant role in particle formation with upper Hofmeister series salts containing strongly hydrated anions yielding particles with a low polydispersity index (PDI) while weaker anions resulted in aggregated particles with high PDIs. Sonication power had minimal effect on mean particle size, however, it significantly reduced polydispersity. Protein loading efficiencies in chitosan nano/microparticles, which ranged from 14.3% to 99.2%, were inversely related to the hydration strength of precipitant salts, protein molecular weight and directly related to the concentration and molecular weight of chitosan. Protein release rates increased with particle size and were generally inversely related to protein molecular weight. This study demonstrates that chitosan nano/microparticles with high protein loading efficiencies can be engineered with well-defined sizes and controllable release kinetics through manipulation of specific formulation parameters. (C) 2014 Elsevier Ltd. All rights reserved.

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