Review
Engineering, Biomedical
Chiara Rinoldi, Ewa Kijenska-Gawronska, Ali Khademhosseini, Ali Tamayol, Wojciech Swieszkowski
Summary: Injuries to tendons and ligaments are common and have a significant impact on daily life. Current treatment strategies are sometimes inadequate, leading to the development of tissue engineering and regenerative medicine approaches aiming to stimulate regeneration. The design of artificial scaffolds with tailored mechanical properties is crucial for restoring functionality in such cases.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Keisuke Fukada, Koji Tachibana, Yuta Kurashina, Yosuke Kaneko, Tatsuaki Matsumoto, Takeshi Miyamoto, Yasuo Niki, Masaya Nakamura, Hiroaki Onoe
Summary: This paper presents a scalable microfiber-shaped tissue for macroscale tendon tissue reconstruction in vitro. The microfiber-shaped tendon-like tissues maintained their morphology and showed proper expression of tenomodulin and actin filaments. A macro-scale tendon tissue assembly was successfully constructed by bundling the microfiber-shaped tendon-like tissues, allowing for uniform cell distribution.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Cell & Tissue Engineering
Vincent P. Willard, Holly A. Leddy, Daniel Palmer, Chia-Lung Wu, Wolfgang Liedtke, Farshid Guilak
Summary: The expression and activation of TRPV4 in chondrogenesis play a crucial role in regulating cartilaginous matrix production through modulation of chondrogenic gene markers and Ca2+ signaling. Understanding the involvement of TRPV4 in cartilage development may lead to new therapeutic strategies for cartilage-related diseases and accelerate stem cell differentiation for tissue engineering purposes.
Review
Cell & Tissue Engineering
Wangwang Luo, Yang Wang, Qing Han, Zhonghan Wang, Jianhang Jiao, Xuqiang Gong, Yang Liu, Aobo Zhang, Han Zhang, Hao Chen, Jincheng Wang, Minfei Wu
Summary: This review discusses recent advances in enthesis tissue engineering, particularly the use of biological scaffolds, and provides perspectives on future directions in this field.
JOURNAL OF TISSUE ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Shabnam Anjum, Ting Li, Mohammad Saeed, Qiang Ao
Summary: Tissue engineering has emerged as a primary research topic for treating diseased or damaged tendon/ligament tissue, and decellularized extracellular matrix (dECM) scaffolds show promise for restoring tissue function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biophysics
M. R. Poorna, R. Jayakumar, Jyh-Ping Chen, Ullas Mony
Summary: Induced pluripotent stem cells (iPSCs) have great potential in regenerative medicine, and improving culture methods, such as using hydrogels as substrates, can support iPSC adhesion, proliferation, and differentiation. Using hydrogels may help generate large numbers of clinical-grade cells for potential clinical applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Biotechnology & Applied Microbiology
Zeqing Zhao, Jin Liu, Michael D. Weir, Abraham Schneider, Tao Ma, Thomas W. Oates, Hockin H. K. Xu, Ke Zhang, Yuxing Bai
Summary: This review article presents the recent advances in PDLSC-based bone regeneration and periodontal tissue regeneration. By combining PDLSCs with various materials and scaffolds, as well as applying different osteoinductive agents and strategies, PDLSCs demonstrate the potential for bone and periodontal tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Amit Gruber, Oded Edri, Shany Glatstein, Idit Goldfracht, Irit Huber, Gil Arbel, Amira Gepstein, Snizhanna Chorna, Lior Gepstein
Summary: This study aimed to develop high-resolution optogenetic approaches to modulate electrical activity in 2D and 3D cardiac tissue models derived from human induced pluripotent stem cell cardiomyocytes. The results demonstrated the ability to control the conduction and contractile properties of cardiac cells using optogenetics, and to use light-controllable techniques for cardiac resynchronization therapy and arrhythmia termination. This approach has important applications in cardiac physiology, pathophysiology, and arrhythmia treatment.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Review
Engineering, Biomedical
Tingyun Lei, Tao Zhang, Wei Ju, Xiao Chen, Boon Chin Heng, Weiliang Shen, Zi Yin
Summary: This review discusses the challenges and strategies for tendon/ligament-to-bone healing, including biomimetic materials, growth factors, and stem cells. The importance of structure-function relationship, growth factor concentration, and stem cell application in the healing process is highlighted. Unmet needs and emerging strategies in tendon/ligament-to-bone regeneration are also discussed.
BIOACTIVE MATERIALS
(2021)
Article
Engineering, Biomedical
Ratna Varma, Alba E. Marin-Araujo, Sara Rostami, Thomas K. Waddell, Golnaz Karoubi, Siba Haykal
Summary: Utilizing human induced pluripotent stem cells (hiPSCs) to engineer airway epithelial patches for transplantation has shown success in airway reconstruction, providing a significant advancement in this field.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Cell & Tissue Engineering
Yuki Fujisawa, Lu Ming, Daisuke Yamada, Tomoka Takao, Takeshi Takarada
Summary: The study established an hPSC-derived MKX-tdTomato reporter cell line and developed an efficient induction method. MKX-tdTomato(+) cells expressed tendon-specific markers and can be used for studying the development or regeneration of tendon tissue.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Engineering, Biomedical
Yexin Li, Ting Deng, Dilihumaer Aili, Yang Chen, Weihong Zhu, Qian Liu
Summary: This review summarizes the application of cell sheet technology in the treatment of tendon and ligament injuries, focusing on the effects of stem cell-derived sheets in mitigating inflammation and promoting healing. It also discusses the prerequisites for future clinical translation.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Thomas Distler, Ines Lauria, Rainer Detsch, Clemens M. Sauter, Farina Bendt, Julia Kapr, Stephan Ruetten, Aldo R. Boccaccini, Ellen Fritsche
Summary: Biodegradable hydrogels composed of oxidized alginate, gelatin, and laminin promote neuronal differentiation and growth of human induced pluripotent stem cell (hiPSC) derived neurospheres in three-dimensional (3D) culture. The addition of laminin to the hydrogels enhances neuron migration from neurospheres to the bulk matrix, leading to a significant increase in the number of migrated neurons. This study highlights the importance of laminin in tailoring oxidized alginate-gelatin hydrogels for 3D neuronal cell culture applications.
Review
Biochemistry & Molecular Biology
Shudong Zhao, Jishizhan Chen, Lei Wu, Xin Tao, Naheem Yaqub, Jinke Chang
Summary: Skeletal muscle, responsible for crucial functions in the body, can suffer from severe injuries leading to volumetric muscle loss (VML). Tissue-engineered muscles using induced pluripotent stem cells (iPSCs) offer a potential solution to restore muscle function. iPSCs, with their pluripotency and self-renewal capacity, can be used to construct tissue-engineered artificial skeletal muscles for various applications. These iPSC-based models also have the potential to revolutionize drug discovery and reduce reliance on animal models. This review provides an overview of iPSCs in tissue-engineered skeletal muscles, discussing advancements, challenges, and considerations for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Saeid Bahrami, Atefeh Solouk, Delphine Duprez, Hamid Mirzadeh
Summary: Scaffold microstructure significantly influences the response of mesenchymal stem cells, with aligned nanofiber scaffolds enhancing tendon and ligament regeneration at the cellular level. While all scaffold types support cell behavior similarly, aligned nanofibers notably upregulate tendon-related markers expression.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Patrick T. Lee, Wan-Ju Li
JOURNAL OF CELLULAR BIOCHEMISTRY
(2017)
Article
Engineering, Biomedical
Yong-Chao Jiang, Hong-Li Jiao, Ming-Song Lee, To Wang, Lih-Sheng Turng, Qian Li, Wan-Ju Li
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2018)
Article
Biochemistry & Molecular Biology
Ming-Song Lee, Jesse Wang, Huihua Yuan, Hongli Jiao, Tsung-Lin Tsai, Matthew W. Squire, Wan-Ju Li
Article
Cell & Tissue Engineering
Jesse F. Wang, Ming-Song Lee, Tsung-Lin Tsai, Ellen M. Leiferman, Darrin J. Trask, Matthew W. Squire, Wan-Ju Li
STEM CELLS TRANSLATIONAL MEDICINE
(2019)
Article
Multidisciplinary Sciences
Matthew J. Stebbins, Benjamin D. Gastfriend, Scott G. Canfield, Ming-Song Lee, Drew Richards, Madeline G. Faubion, Wan-Ju Li, Richard Daneman, Sean P. Palecek, Eric Shusta
Review
Orthopedics
Wan-Ju Li, Hongli Jiao, Brian E. Walczak
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2019)
Article
Orthopedics
Jun Lu, Connie S. Chamberlain, Ming-liang Ji, Erin E. Saether, Ellen M. Leiferman, Wan-Ju Li, Ray Vanderby
AMERICAN JOURNAL OF SPORTS MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Robert Weishar, Ming-Song Lee, Gianluca Fontana, Peiman Hematti, Wan-Ju Li
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Huihua Yuan, Xiaolei Li, Ming-Song Lee, Zhuojun Zhang, Biyun Li, Hongyun Xuan, Wan-Ju Li, Yanzhong Zhang
Summary: The study developed highly-aligned poly(L-lactic acid) fibers decorated with type 1 collagen and chondroitin sulfate, which showed enhanced tenogenic differentiation of human mesenchymal stem cells in vitro. The biomimetic fibers promoted cell spreading and proliferation, increased expression of tendon-associated genes and proteins, and activated the TGF-beta signaling pathway. Animal study validated the promoting role of these fibers in tendon regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biophysics
Matthew M. Grondin, Fang Liu, Michael F. Vignos, Alexey Samsonov, Wan-Ju Li, Richard Kijowski, Corinne R. Henak
Summary: This study demonstrated that fast relaxing macromolecular bound water component fraction (FF) measured using mcDESPOT at 3.0 T is significantly correlated with energy dissipation, linear modulus, and proteoglycan content in cartilage. Other mcDESPOT parameters showed limited correlation with mechanical and chemical properties of cartilage.
JOURNAL OF BIOMECHANICS
(2021)
Article
Multidisciplinary Sciences
Ming-Song Lee, Matthew J. Stebbins, Hongli Jiao, Hui-Ching Huang, Ellen M. Leiferman, Brian E. Walzack, Sean P. Palecek, Eric Shusta, Wan-Ju Li
Summary: Chondrocytes derived from pluripotent stem cells through mesodermal and ectomesodermal differentiation show differences in activities and functions, with NCC-Chs being closer to native chondrocytes and exhibiting increased matrix production and stiffness compared to MC-Chs.
Editorial Material
Biotechnology & Applied Microbiology
Yixuan Amy Pei, Yufeng Dong, Tong-Chuan He, Wan-Ju Li, Wei Seong Toh, Ming Pei
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Orthopedics
Brian E. Walczak, Hongli Jiao, Ming-Song Lee, Wan-Ju Li
Summary: Our study demonstrates that epigenetic reprogramming enhances the phenotypic characteristics and functions of MSCs harvested from OA patients. Additionally, reprogrammed MSCs show an improved potential for articular cartilage repair. The reprogramming strategy provides a potential solution to the challenge of variation in MSC quality.
Article
Multidisciplinary Sciences
Ngai Ting Chan, Ming-Song Lee, Yidan Wang, Jacques Galipeau, Wan-Ju Li, Wei Xu
Summary: This study reveals that CTR9 regulates the osteochondral lineage differentiation of hMSCs by counteracting EZH2 activity and regulating BMP-2 expression. CTR9 knockdown in hMSCs leads to defects in osteogenic differentiation, which can be partially rescued by EZH2 inhibitors. CTR9 also regulates the secretion and function of BMP-2.
Article
Cell & Tissue Engineering
Hongli Jiao, Brian E. Walczak, Ming-Song Lee, Madeleine E. Lemieux, Wan-Ju Li
Summary: Cellular reprogramming can reverse aging of cells by altering the activities of regulatory networks associated with inflammation and proliferation. The expression of GATA6 in reprogrammed cells is attenuated, leading to an increase in sonic hedgehog signaling and FOXP1 expression, which ameliorates cellular hallmarks of aging. GATA6 is identified as a critical regulator of aging-related activities in MSCs, controlling the downstream pathways to modulate cellular senescence.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.