期刊
BIOMATERIALS
卷 35, 期 28, 页码 8134-8143出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.06.009
关键词
Fibrin; Gene therapy; Gold; Hydrogel; Infrared spectrum; Scaffold
资金
- Fondo de Investigaciones Sanitarias (FIS, Spanish Ministry of Economy and Competitiveness, MINECO, Spain) [PI12/01698]
- Spanish Ministry of Science and Innovation (MICINN) [MAT2011-24988, MAT2011-24797]
- United States Department of Defense [OR090134]
- National Institutes of Health [R01 DE013386]
- HSF Pharmaceuticals S.A.
- program Sara Borrell from FIS
- NIH [T32DE007057-34]
- Program 12 from Comunidad de Madrid (Spain)
We investigated whether near-infrared (NIR) light could be employed for patterning transgene expression in plasmonic cell constructs. Hollow gold nanoparticles with a plasmon surface band absorption peaking at similar to 750 nm, a wavelength within the so called tissue optical window, were used as fillers in fibrin-based hydrogels. These composites, which efficiently transduce NIR photon energy into heat, were loaded with genetically-modified cells that harbor a heat-activated and ligand-dependent gene switch for regulating transgene expression. NIR laser irradiation in the presence of ligand triggered 3-dimensional patterns of transgene expression faithfully matching the illuminated areas of plasmonic cell constructs. This non-invasive technology was proven useful for remotely controlling in vivo the spatiotemporal bioavailability of transgenic vascular endothelial growth factor. The combination of spatial control by means of NIR irradiation along with safe and timed transgene induction presents a high application potential for engineering tissues in regenerative medicine scenarios. (C) 2014 Elsevier Ltd. All rights reserved.
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