Article
Biochemistry & Molecular Biology
Mengke Han, Samuel Evans, Sanam Mustafa, Steven Wiederman, Heike Ebendorff-Heidepriem
Summary: This article investigates the effective intracellular delivery of nanomaterials using microelectrophoresis in biological research. The study found that the amount of delivered nanomaterials, and specifically quantum dots (QDs), strongly correlates with cell viability, which can be controlled by adjusting the ejection duration. Furthermore, the research reveals that microelectrophoretically delivered QDs distribute homogeneously and exhibit pure Brownian diffusion inside the cytoplasm, showcasing great potential for studying dynamic intracellular events.
BIOELECTROCHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Dominyka Dapkute, Marijus Pleckaitis, Danute Bulotiene, Dainius Daunoravicius, Ricardas Rotomskis, Vitalijus Karabanovas
Summary: A complex composed of photoluminescent quantum dots and a photosensitizer chlorin e6 was constructed to obtain multifunctional nanoparticles for cancer diagnostic and therapeutic purposes. In vitro and in vivo studies demonstrated the potential of the complex-loaded MSCs in transporting theranostic nanoparticles and inducing cancer cell death, leading to increased survival of tumor-bearing mice and slowed down tumor progression and metastases.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Peiwen Fu, Yumeng Guo, Yanan Luo, Michael Mak, Jianguo Zhang, Wenrong Xu, Hui Qian, Zhimin Tao
Summary: This study presents a nano-scale fluorescent off-on complex encapsulated by small extracellular vesicles (sEVs) for real-time visualization and evaluation of gene therapy efficiency in cancer. The results demonstrate that the complex is able to activate the turn-on fluorescent signal and exhibit the longest retention time in vivo, promoting cancer apoptosis both in vitro and in vivo.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Zahra Zahed, Raha Hadi, Gholamhassan Imanzadeh, Zainab Ahmadian, Sasan Shafiei, Amin Zaki Zadeh, Hanie Karimi, Amirhossein Akbarzadeh, Mahmoud Abbaszadeh, Laleh Saleh Ghadimi, Hossein Samadi Kafil, Fahimeh Kazeminava
Summary: This article reviews the latest research developments in gene delivery, with a focus on the potential of quantum dots as a tool for gene delivery. By evaluating the physicochemical characteristics of quantum dots, researchers believe that they can be used in biosensing imaging, drug delivery, and gene delivery applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Chemistry, Multidisciplinary
Emel Kirbas Cilingir, Meghana Sankaran, Jordan M. Garber, Frederic Anthony Vallejo, Mattia Bartoli, Alberto Tagliaferro, Steven Vanni, Regina M. Graham, Roger M. Leblanc
Summary: Carbon dots (CDs) have gained attention for their biocompatibility and tunable photoluminescence, but further analysis is needed before their use in clinical trials. A new type of carbon nitride dot (CND) has been found to selectively uptake cancer cells due to structural similarities with glutamine. The study investigates the effects of surface structural differences on the cellular uptake of CNDs and finds that the uptake mechanism is linked with ASCT2 and LAT1 transporters. CNDs show promise for selective cancer cell targeting by utilizing highly expressed transporters on cancer cells.
Article
Nanoscience & Nanotechnology
Suwan Liu, Miaomiao Zhang, Huijun Yu, Xin Sun, Qianzhe Li, Mengyu Yang, Xiaonan Qiu, Hang Su, Aihua Gong, Fengyi Du
Summary: The study developed an immunoinducible material using carbon dots and hydrogel, which induced immunogenic tumor cell death and enhanced the maturation and antigen presentation ability of immune cells through photothermal therapy.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Dito Anurogo, Nova Yuli Prasetyo Budi, Mai-Huong Thi Ngo, Yen-Hua Huang, Jeanne Adiwinata Pawitan
Summary: Hereditary anemia presents with various manifestations, and current management strategies are unsatisfactory. Gene-corrected hematopoietic stem cell transplantation may offer promising outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Ganji Seeta Rama Raju, Begum Dariya, Sathish Kumar Mungamuri, Gayathri Chalikonda, Sung-Min Kang, Ishaq N. Khan, Pinninti Santosh Sushma, Ganji Purnachandra Nagaraju, Eluri Pavitra, Young-Kyu Han
Summary: Cancer is a deadly disease with limitations in traditional therapies. Nanotechnology, as an emerging science, provides more effective cancer treatments by overcoming biomedical and biophysical barriers.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Biochemical Research Methods
Giulia Pais, Giulio Spinozzi, Daniela Cesana, Fabrizio Benedicenti, Alessandra Albertini, Maria Ester Bernardo, Bernhard Gentner, Eugenio Montini, Andrea Calabria
Summary: Longitudinal clonal tracking studies in gene therapy applications require comprehensive and high-throughput analysis tools. ISAnalytics is a new R package that allows users to assess safety and efficacy of treatments and analyze hematopoietic stem cell biology using vector integration sites as markers. It provides efficient data structures, reproducibility, and analytical control, making it suitable for various clonal tracking applications.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Biochemical Research Methods
Giulia Pais, Giulio Spinozzi, Daniela Cesana, Fabrizio Benedicenti, Alessandra Albertini, Maria Ester Bernardo, Bernhard Gentner, Eugenio Montini, Andrea Calabria
Summary: Comprehensive and high-throughput clonal tracking studies using vector integration sites as markers of cellular identity are supported by ISAnalytics, a new R package. ISAnalytics addresses the computational challenges of monitoring patients over a long-term follow-up and provides a wide range of functionalities for assessing safety and efficacy and supporting stem cell biology. The package integrates seamlessly with laboratory workflows and standard procedures, offering efficient data structures and interactive web reports for reproducibility and analytical control.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Chemistry, Multidisciplinary
Suman Nayak, Prolay Das
Summary: The research demonstrates the first covalent conjugation of plasmid with CD, forming toroidal DNA condensates. The binding of CD-pUC19 conjugate with spermine is endothermic and driven by entropy, with the condensed plasmid displaying severe conformational stress and better transfection ability. While the CDs in the condensates shield themselves from excitation, they still emit reactive oxygen species under visible light exposure, compromising decondensation and cell viability, warranting caution in their universal use as nonviral transfecting agents.
Article
Chemistry, Physical
Alexandra Karagianni, Nikos G. Tsierkezos, Maurizio Prato, Mauricio Terrones, Konstantinos V. Kordatos
Summary: Photodynamic Therapy (PDT) is a non-invasive cancer treatment modality using photosensitizer drug, light, and oxygen. Conventional photosensitizers have limitations, which can be overcome by using nanomaterials such as Carbon Quantum Dots (CQDs) and Graphene Quantum Dots (GrQDs) due to their excellent photoluminescence properties, biocompatibility, and hydrophilicity. This review article summarizes the main features of PDT and carbon-based quantum dots and discusses their applications in cancer diagnosis and treatment.
Article
Chemistry, Multidisciplinary
Minchul Ahn, Jaekwang Song, Byung Hee Hong
Summary: NGQDs, as a novel gene delivery platform, have shown efficient delivery and transfection of genes by forming complexes with mRNAs and pDNAs. With comparable transfection efficiency to LNPs, NGQDs exhibit outstanding stability and low toxicity, making them a promising universal gene delivery platform surpassing LNPs and viral vectors.
Article
Chemistry, Multidisciplinary
Shu-Lan Li, Peng Jiang, Siyu Hua, Feng-Lei Jiang, Yi Liu
Summary: This study reported a simple one-step synthesis method for ultrasmall Zn-doped Cu2S QDs with high CDT activity and fluorescence imaging capability. The QDs were able to specifically inhibit cancer cell growth and induce apoptosis via a Fenton-like reaction, showing potential as multi-functional theranostic agents.
Article
Chemistry, Multidisciplinary
Wanchuan Ding, Xuan Yang, Huoyue Lin, Zixing Xu, Jun Wang, Jie Dai, Can Xu, Feng Chen, Xiaowei Wen, Weiran Chai, Gang Ruan
Summary: Hard-to-transfect cells, such as BMSCs, present challenges in intracellular delivery due to vesicle trapping. We found that coating nanoparticles with PDS1 can effectively avoid vesicle trapping in BMSCs and significantly enhance transfection efficiency and osteoblastic differentiation. Mechanistic studies suggest that higher cholesterol content in BMSCs' plasma membranes may contribute to the difficulty of vesicle escape.
Article
Chemistry, Multidisciplinary
Eun-Jin Go, Dasom Yang, WonHyoung Ryu, Hong Jae Chon, Chan Kim, Kyung-Soon Park, Dong-Hyun Kim, Dong Keun Han, Wooram Park
Summary: Electrical ablation (EA) is a non-thermal technique that induces immunogenic cell death and can synergize with cancer immunotherapy. This study identified optimal voltage and pulse conditions for inducing immunogenic cell death, which can be combined with cancer immunotherapy for potential clinical applications.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Cell & Tissue Engineering
Joon-Kyu Kim, Eun-Jin Go, Kyoung-Won Ko, Hyeon-Ji Oh, Jieun Han, Dong Keun Han, Wooram Park
Summary: The study demonstrated that encapsulating RA-MH in PLGA microspheres significantly enhanced the acid-neutralizing effect and biocompatibility, while also showing superior anti-inflammatory properties compared to PLGA microspheres. This technology has great potential for applications in drug delivery and tissue engineering to treat various incurable diseases in the future.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Engineering, Biomedical
Shin-Young Kim, Ginam Han, Da-Bin Hwang, Dong-Hoon Won, Yoo-Sub Shin, Changuk Kim, Jeon Min Kang, Jung-Hoon Park, Hyun-Do Jung, Wooram Park, Jun-Won Yun
Summary: This study evaluates the in vivo safety and usability of a 3D-printed implantable drug delivery device for ALF treatment. The results show that the device is safe for use and can deliver various active pharmaceutical ingredients to the liver tissue for the treatment of acute liver failure.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Pharmacology & Pharmacy
Hayoon Jeong, Wooram Park, Dong-Hyun Kim, Kun Na
Summary: Advances in chemical and material sciences have led to the development of various light-activated photosensitizers for effective photodynamic therapy and photothermal therapy. Intelligent dynamic nanoassemblies activated in a tumor environment have shown potential for targeting diseased tissues effectively and increasing therapeutic effectiveness while minimizing side effects.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Review
Cell & Tissue Engineering
Seungyong Shin, Jiyoung Lee, Jieun Han, Fangyuan Li, Daishun Ling, Wooram Park
Summary: Cancer treatment faces challenges due to the resistance mechanism of cancer cells related to the tumor microenvironment. Utilizing functional nanoparticles to modulate the tumor microenvironment can effectively treat refractory cancer, potentially maximizing cancer treatment efficiency when combined with modern cancer therapeutics.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Yubeen Park, Dong-Sung Won, Ga-Hyun Bae, Dae Sung Ryu, Jeon Min Kang, Ji Won Kim, Song Hee Kim, Chu Hui Zeng, Wooram Park, Sang Soo Lee, Jung-Hoon Park
Summary: The efficacy of silver nanoparticles-coated self-expandable metal stents (SEMSs) in inhibiting tissue hyperplasia and bacterial growth caused by radiofrequency ablation with stent placement was investigated in a rabbit model. The results demonstrated that the AgNPs-coated SEMS significantly suppressed bacterial activity and tissue hyperplasia, suggesting that this approach could be a promising treatment option for thermal damage and stent-related complications.
Article
Chemistry, Multidisciplinary
Jun-Hyeok Han, Ha Eun Shin, Jiyoung Lee, Jeon Min Kang, Jung-Hoon Park, Chun Gwon Park, Dong Keun Han, Ik-Hwan Kim, Wooram Park
Summary: In this study, a novel nanoparticle in combination with irreversible electroporation (IRE) was developed for effective cancer immunotherapy. The nanoparticle, synthesized and coated, can be effectively internalized by antigen-presenting cells and trigger an immune response. Notably, the nanoparticle demonstrated longer retention time in the tumor compared to the free drug. Tailored IRE expanded the ablation zone and enhanced therapeutic efficacy. Ultimately, the combined treatment successfully inhibited tumor growth and prolonged survival.
Article
Engineering, Biomedical
Eunyoung Jeon, Jeon Min Kang, Ga-Hyun Bae, Chu Hui Zeng, Seungyong Shin, Byeongdu Lee, Wooram Park, Jung-Hoon Park, Joonseok Lee
Summary: A flexible 3D nanonetworked silica film (NSF) was developed as a drug delivery platform for self-expandable metallic stents (SEMSs), capable of withstanding mechanical stress during dynamic expansion and improving therapeutic effects.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Hyunjun Choi, Bongseo Choi, Jun-Hyeok Han, Ha Eun Shin, Wooram Park, Dong-Hyun Kim
Summary: A ROS-responsive cleavable hierarchical metallic supra-nanostructure (HMSN) is reported, which shows significant structural deformation in the presence of ROS and demonstrates potential in cancer treatment and other biomedical applications.
Article
Biochemistry & Molecular Biology
Hye Won Lee, Hee Seung Seo, Seon-Yong Yeom, Se-Na Kim, Cho Rim Kim, Dae-Hwan Park, Wooram Park, Young Bin Choy, Chun Gwon Park, Seong Il Seo
Summary: The use of CZ-loaded PLGA nanoparticles as an adjuvant therapy for post-nephrectomy metastasis shows promising results in inhibiting tumor growth and metastasis in renal cell carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Physical
Joonghak Lee, Hee Seung Seo, Wooram Park, Chun Gwon Park, Yukwon Jeon, Dae-Hwan Park
Summary: This review summarizes the recent advances in LDH nanohybrids for cancer therapeutic strategies, including gene therapy, chemotherapy, immunotherapy, and combination therapy. LDH hybridized with therapeutic agents plays a significant role in cancer imaging and therapy.
Review
Cell & Tissue Engineering
Seungyong Shin, Pyunghwajun Lee, Jieun Han, Se-Na Kim, Jaesung Lim, Dae-Hwan Park, Taejong Paik, Junhong Min, Chun Gwon Park, Wooram Park
Summary: Adoptive cell therapy using CAR-engineered T cells has shown promise in hematological cancer treatment. However, its limited effectiveness for solid tumors, complex processes, and high manufacturing costs are major limitations. Nanotechnology offers an alternative approach by utilizing nanoparticle-based CAR therapy, which can target specific cells and overcome some limitations. This review discusses the use of nanoparticle-based CAR immune cell therapy and future perspectives on immune cell reprogramming.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Eun-Jin Go, Hannah Yang, Wooram Park, Seung Joon Lee, Jun-Hyeok Han, So Jung Kong, Won Suk Lee, Dong Keun Han, Hong Jae Chon, Chan Kim
Summary: Positively charged fusogenic liposomes loaded with a STING agonist are designed for systemic delivery and preferentially target the tumor microenvironment. This delivery system activates STING and induces robust anti-tumor T cell immunity within the tumor microenvironment.
Article
Materials Science, Biomaterials
Wooram Park, Seok-Jo Kim, Paul Cheresh, Jeanho Yun, Byeongdu Lee, David W. Kamp, Dong-Hyun Kim
Summary: Mitochondria serve as crucial regulators of cancer cell death pathway, and the use of magnetic nano-transducers in inducing mitochondrial dysfunction remotely to kill cancer cells shows promising results in liver cancer animal models. This study contributes to the advancement of nanoparticle-mediated cancer therapy and biological tools for research purposes.
BIOMATERIALS SCIENCE
(2021)
Review
Materials Science, Biomaterials
Tarek M. Bedair, Yun Heo, Jungju Ryu, Hanan M. Bedair, Wooram Park, Dong Keun Han
Summary: Magnesium ceramics exhibit beneficial characteristics for human biology, including anti-inflammatory, antibacterial, antiviral, and anti-cancer effects. They have been extensively utilized in biomedical fields, enhancing re-endothelialization in vascular stents and promoting tissue regeneration for bone, cartilage, and kidney. Researchers can identify optimal synthetic conditions and select appropriate modification protocols for magnesium ceramics to realize their desirable physicochemical properties and biological benefits.
BIOMATERIALS SCIENCE
(2021)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
