期刊
BIOMATERIALS
卷 35, 期 25, 页码 6822-6828出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.04.074
关键词
Renal regeneration; Scaffold; Partial nephrectomy; Stem/progenitor cells
资金
- National Natural Science Foundation of China [81071576]
- Natural Science Foundation of Zhejiang Province [LY12H15002, LY14H050005]
Renal regeneration approaches offer great potential for the treatment of chronic kidney disease, but their availability remains limited by the clinical challenges they pose. In the present study, we used continuous detergent perfusion to generate decellularized (DC) rat kidney scaffolds. The scaffolds retained intact vascular trees and overall architecture, along with significant concentrations of various cytokines, but lost all cellular components. To evaluate its potential in renal function recovery, DC scaffold tissue was grafted onto partially nephrectomized rat kidneys. An increase of renal size was found, and regenerated renal parenchyma cells were observed in the repair area containing the grafted scaffold. In addition, the number of nestin-positive renal progenitor cells was markedly higher in scaffold-grafted kidneys compared to controls. Moreover, radionuclide scan analysis showed significant recovery of renal functions at 6 weeks post-implantation. Our results provide further evidence to show that DC kidney scaffolds could be used to promote renal recovery in the treatment of chronic kidney disease. (C) 2014 Elsevier Ltd. All rights reserved.
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