Review
Engineering, Biomedical
Duoyi Zhao, Tongtong Zhu, Jie Li, Liguo Cui, Zhiyu Zhang, Xiuli Zhuang, Jianxun Ding
Summary: This article comprehensively reviews the research and applications of artificial bone-substitute materials based on PLGA and its composites, discussing their potential and challenges in bone regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Ying Mao, Robert Guidoin, Yan Li, Gaetan Brochu, Ze Zhang, Lu Wang
Summary: Blending L-alpha-phosphatidylcholine (PC) with PLGA to yield hybrid scaffolds alters the physical properties and wettability, affecting biodegradability and biocompatibility. The addition of PC decreases fiber diameter and increases porosity and hydrophilicity, although it also reduces mechanical strength of the scaffolds.
MATERIALS & DESIGN
(2021)
Review
Biochemistry & Molecular Biology
Nor Idayu A. Rahman, Chung Liang Lam, Nadiah Sulaiman, Nur Atiqah Haizum Abdullah, Fazlina Nordin, Shahrul Hisham Zainal Ariffin, Muhammad Dain Yazid
Summary: Muscular dystrophy is a group of hereditary muscle disorders caused by gene mutations that lead to muscle wasting and loss of regeneration. Pax7, a marker for myogenesis, is regulated by various signaling pathways and plays a crucial role in muscle research. This review explores the expression of Pax7 in different signaling pathways and offers insight into potential strategies for improving dystrophic muscle through manipulating Pax7 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Juan M. Fernandez-Costa, Xiomara Fernandez-Garibay, Ferran Velasco-Mallorqui, Javier Ramon-Azcon
Summary: Muscular dystrophies are a group of disabling disorders characterized by muscle degeneration, for which there is currently no effective cure. Recent advances in bioengineering have led to the development of bioartificial muscles and tools for drug screening in skeletal muscle tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Polymer Science
Yi-Hsun Yu, Shih-Jyun Shen, Yung-Heng Hsu, Ying-Chao Chou, Ping-Chun Yu, Shih-Jung Liu
Summary: In this study, a novel nanofibrous scaffold was developed for repairing ruptured Achilles tendons. It integrated multiple biomolecules layer by layer and achieved sustained release to enhance tendon healing. The experimental results demonstrated that the scaffold significantly improved the load-to-failure of the repaired tendons.
Article
Engineering, Biomedical
Lizhe Wang, Yaxin Li, Shuai Jiang, Zhihao Zhang, Sinan Zhao, Yuru Song, Jie Liu, Fei Tan
Summary: In order to improve the characteristics of alginate as a bone scaffold, a novel injectable tissue engineering scaffold containing poly(lactic-co-glycolic acid) (PLGA) microspheres and alginate was fabricated. PLGA microspheres containing magnesium (PMg) were developed by loading MgCO3 and MgO into PLGA microspheres. The addition of PMg effectively regulated the physicochemical properties of alginate hydrogels and improved cell attachment, proliferation, and osteogenesis.
BIOMEDICAL MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Janine Tomasch, Babette Maleiner, Philipp Heher, Manuel Rufin, Orestis G. Andriotis, Philipp J. Thurner, Heinz Redl, Christiane Fuchs, Andreas H. Teuschl-Woller
Summary: This study aimed to investigate the influence of apparent elastic properties of fibrin scaffolds on myoblasts and evaluate if those effects differ between murine and human cells. The results showed that the elastic modulus of fibrin hydrogels had different effects on myoblast proliferation and differentiation, with a more pronounced impact on human cells in a 3D environment compared to 2D. Furthermore, the differentiation potential of human myoblasts was significantly impaired when incorporated into 3D gels. These findings provide insights into the cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches, highlighting the need for adjusting 3D culture parameters when working with human cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Oncology
Xuekai Xiong, Hongbo Gao, Yayu Lin, Vijay Yechoor, Ke Ma
Summary: The deficiency of Rev-erb alpha in mdx mice promotes regenerative myogenic response, ameliorates muscle damage, and enhances the regenerative capacity of dystrophic muscle.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Taegyun Yeo, Young-Gwang Ko, Eun Jin Kim, Oh Kyoung Kwon, Ho Yun Chung, Oh Hyeong Kwon
Summary: 3D-printed PGA/HAp scaffolds demonstrate significant potential for promoting bone tissue regeneration with higher compressive strength and biodegradability. Clinical experiments show a higher rate of bone regeneration and bone density.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Geriatrics & Gerontology
Mina Shahriyari, Md Rezaul Islam, Sadman M. Sakib, Malte Rinn, Anastasia Rika, Dennis Krueger, Lalit Kaurani, Verena Gisa, Mandy Winterhoff, Harithaa Anandakumar, Orr Shomroni, Matthias Schmidt, Gabriela Salinas, Andreas Unger, Wolfgang A. Linke, Jana Zschuentzsch, Jens Schmidt, Rhonda Bassel-Duby, Eric N. Olson, Andre Fischer, Wolfram-Hubertus Zimmermann, Malte Tiburcy
Summary: This study successfully established methods for deriving skeletal muscle cells from human pluripotent stem cells and engineered a functional human skeletal muscle organoid and engineered skeletal muscle with regeneration-competent satellite-like cells. Contractile performance of the engineered muscle was further enhanced by thyroid hormone treatment.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Review
Cell Biology
Lu Yan, Alejandra Rodriguez-delaRosa, Olivier Pourquie
Summary: The differentiation of human pluripotent stem cells into muscle cells has significant implications for studying human muscle development and treating muscular diseases. Through various differentiation strategies and bioengineering approaches, human PSCs can be utilized to generate muscle tissue and explore potential clinical applications.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Polymer Science
Adriana Cristina Motta, Moema Hausen, Talita Stessuk, Elaine Conceicao de Oliveira, Anna Maria G. Melero, Eliana Aparecida de Rezende Duek
Summary: The study synthesized PDLGA (67:23) membranes and verified its degradation properties through cell culture, showing early degradation characteristics. Adipose-derived mesenchymal stem cells seeded in PDLGA membranes remained undifferentiated, providing data support for further research.
Article
Multidisciplinary Sciences
Ziad Al Tanoury, John F. Zimmerman, Jyoti Rao, Daniel Sieiro, Harold M. McNamara, Thomas Cherrier, Alejandra Rodriguez-delaRosa, Aurore Hick-Colin, Fanny Bousson, Charlotte Fugier-Schmucker, Fabio Marchiano, Bianca Habermann, Jerome Chal, Alexander P. Nesmith, Svetlana Gapon, Erica Wagner, Vandana A. Gupta, Rhonda Bassel-Duby, Eric N. Olson, Adam E. Cohen, Kevin Kit Parker, Oliver Pourquie
Summary: This study introduces an in vitro human model to investigate the pathology of Duchenne muscular dystrophy (DMD) and test new therapeutic approaches. The researchers describe an optimized protocol for differentiating human induced pluripotent stem cells (iPSC) to a late myogenic stage, which allows them to replicate classic DMD phenotypes in isogenic DMD-mutant iPSC lines. Treatment with prednisolone significantly improves muscle function in DMD iPSC lines, challenging the prevailing view that its benefits are solely due to anti-inflammatory properties.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Biomedical
Dhanalakshmi Jeyachandran, Monzur Murshed, Lisbet Haglund, Marta Cerruti
Summary: Bone tissue engineering often fails due to lack of oxygen, but using a Bioglass-Poly(lactic-co-glycolic acid@fibrin) construct may overcome this issue by promoting the formation of a cartilage template that can survive in hypoxic environments. The construct induces hypertrophy and matrix mineralization, supporting the different phases of endochondral ossification. This approach may decrease cell culture time and complexity associated with external induction factors, facilitating clinical translation.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Anatomy & Morphology
Romina L. Filippelli, Natasha C. Chang
Summary: DMD is a devastating muscle degenerative disease with no effective cure. Defective expression of the DMD gene and impaired muscle stem cell function contribute to the progression of the disease. Restoring muscle stem cell function may serve as a viable regenerative medicine strategy to mitigate DMD.
CELLS TISSUES ORGANS
(2023)
Article
Chemistry, Multidisciplinary
Seokyoung Bang, Dongha Tahk, Young Hwan Choi, Somin Lee, Jungeun Lim, Seung-Ryeol Lee, Byung-Soo Kim, Hong Nam Kim, Nathaniel S. Hwang, Noo Li Jeon
Summary: A vascularized engineered tissue construct inspired by MPS has been designed for regenerative medicine, allowing long-term cell culture without damage to the capillary network through time-controlled delamination of through-hole membranes. When implanted in a mouse ischemic model, it shows better protection against necrosis and promotes functional recovery compared to other implanted forms.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Suyeong Seo, Seung-Yeol Nah, Kangwon Lee, Nakwon Choi, Hong Nam Kim
Summary: The study introduced an engineered 3D human glioblastoma in vitro platform integrated with a tricultured BBB, allowing for mimicking the physiological environment of brain tumors. The platform showed potential for studying the physiology of the BBB and monitoring drug responses based on the interaction between brain tumor cells and the BBB.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Suyeong Seo, Ji Eun Lee, Kangwon Lee, Hong Nam Kim
Summary: Nanoparticles are considered hazardous to human organs, and there is a growing interest in understanding their biological mechanisms and evaluating cellular responses. Conventional in vitro evaluation of nanomaterial toxicity using 2D-based static models often leads to over- or underestimation of cytotoxicity, as it overlooks physiological microenvironmental factors.
ENVIRONMENTAL SCIENCE-NANO
(2022)
Article
Engineering, Biomedical
Dasom Kim, Kyeong Seob Hwang, Eun U. Seo, Suyeong Seo, Byung Chul Lee, Nakwon Choi, Jonghoon Choi, Hong Nam Kim
Summary: The tumor microenvironment (TME) plays a complex role in tumor growth and drug delivery. A method for inducing controlled tumor angiogenesis by engineering the microenvironment is presented. This vascularized tumor platform can enhance the delivery of anticancer drugs and immune cells, and aid in the development of effective anticancer drugs and cancer immunotherapy.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Wonbeak Yoo, Wonhwa Lee, Hong Nam Kim, Jiyoung Jeong, Hee Ho Park, June Hong Ahn, Dana Jung, Juheon Lee, Ji-su Kim, Seung Whan Lee, Wan-Seob Cho, Seokho Kim
Summary: This study found that highly purified detonation-synthesized nanodiamonds can serve as a therapeutic agent for cytokine release syndrome (CRS) by adsorbing inflammatory cytokines. In an animal model, intravenous injection of DND samples significantly improved survival rates and reduced circulating inflammatory cytokines.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Hyunjung Kim, Hong Nam Kim, Seong Min Kang
Summary: This paper presents a novel fabrication method for a cylindrical double-ring mushroom structure (DMS) and demonstrates its superior structural characteristics and omniphobic properties. The excellent performance of this structure is proven through wettability tests and comparative experiments of transmittance. This low-cost fabrication method, which does not require additional chemical treatment, is expected to have an impact in various application fields.
SURFACES AND INTERFACES
(2022)
Article
Biochemical Research Methods
Seokyoung Bang, Songhyun Lee, Kyeong Seob Hwang, Jongbaeg Kim, Nakwon Choi, Hong Nam Kim
Summary: This study developed a method for axotomy on a three-dimensional neuron-culture platform, which can accurately recapitulate central nervous system injuries. The damaged area could be filled and neurites could regenerate using this platform. Co-culture of astrocytes was also possible without affecting the alignment of axons. This open-access platform is expected to contribute to the development of treatment techniques for central nervous system injuries.
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Hyelim Kim, Boram Son, Eun U. Seo, Miji Kwon, June Hong Ahn, Heungsoo Shin, Gyu Yong Song, Eun Ji Park, Dong Hee Na, Seung-Woo Cho, Hong Nam Kim, Hee Ho Park, Wonhwa Lee
Summary: This article presents a strategy to continuously express therapeutic peptides on the surface of immune cells, using engineered T cells. These chimeric T cells can prevent vascular damage, suppress hypercytokinemia and lung tissue damages, demonstrating potential for treating severe infectious diseases.
Article
Engineering, Biomedical
Yoojin Seo, Seokyoung Bang, Jeongtae Son, Dongsup Kim, Yong Jeong, Pilnam Kim, Jihun Yang, Joon-Ho Eom, Nakwon Choi, Hong Nam Kim
Summary: In the past few decades, the prediction of adverse reactions to drugs mainly relied on 2D in vitro models and animal models. However, with advancements in computational power, in silico models have emerged as a time-efficient and cost-effective alternative. The concept of the physiome has bridged experimental datasets with computational models, leading to the development of 3D in vitro brain models to overcome limitations in experimental data collection from traditional models.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Suyeong Seo, Minjeong Jang, Hwieun Kim, Jong Hwan Sung, Nakwon Choi, Kangwon Lee, Hong Nam Kim
Summary: Air pollution-induced neurodegeneration, including cognitive deficits, neuroinflammation, and disrupted blood-brain barrier, is mediated by intercellular interactions. A brain-on-a-chip platform is used to evaluate diesel exhaust particle(DPE)-induced neurodegeneration and reveal the neurodegeneration cascade involving brain endothelial cells(bECs), microglia, and neurons. Inhibition of intercellular interactions, such as neutralizing GM-CSF, suppressing microglial activation, and scavenging ROS, can prevent neurodegeneration in the model.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Neurosciences
Sukmin Han, Seokyoung Bang, Hong Nam Kim, Nakwon Choi, Sung Hyun Kim
Summary: The central nervous system is composed of different neural circuits, each with its own functions and properties. Studying these circuits is crucial for understanding brain function and neuronal diseases. Microfluidic systems are commonly used for studying neural circuits but require improvement for better modulation and monitoring of circuit properties. This study presents an improved microfluidic device that allows electrical modulation and proper reassembly of neural circuits. The device was shown to be capable of electrically modulating and monitoring the physiological function of neural circuits with genetic indicators for synaptic functionality. Our findings demonstrate that this improved microfluidic platform is an invaluable tool for studying the physiological properties of specific neural circuits.
Review
Biochemical Research Methods
Minjeong Jang, Hong Nam Kim
Summary: Metabolic diseases are a significant global health issue, and understanding inter-organ communication is crucial for their treatment. Organ-on-a-chip systems provide a platform to study metabolic dynamics and inter-organ crosstalk.
Article
Chemistry, Multidisciplinary
Joon Ho Kang, Minjeong Jang, Su Jin Seo, Andrew Choi, Daeeun Shin, Suyoung Seo, Soo Hyun Lee, Hong Nam Kim
Summary: In infectious diseases like sepsis and COVID-19, improving vascular barrier function is crucial. This study shows that osmolarity modulation can effectively enhance vascular barrier function, even in the presence of inflammation. Through the use of 3D human vascular microphysiological systems, it is demonstrated that hyperosmotic exposure for 24-48 hours can significantly improve vascular barrier function, while hypo-osmotic exposure disrupts it. The findings suggest that osmolarity modulation may be a unique therapeutic strategy to protect vascular barrier function and prevent the progression of infectious diseases.
Review
Engineering, Biomedical
Kyeong Seob Hwang, Eun U. Seo, Nakwon Choi, Jongbaeg Kim, Hong Nam Kim
Summary: Viral infections cause damage to various organ systems. Current research mainly focuses on 2D cell culture models and animal models, which have limitations in recapitulating human-specific viral infection pathology. Three-dimensional engineered tissue models have shown potential in replicating infection-associated pathologies.
BIOACTIVE MATERIALS
(2023)
Meeting Abstract
Cell & Tissue Engineering
Hong Nam Kim
TISSUE ENGINEERING PART A
(2022)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.