期刊
BIOMATERIALS
卷 35, 期 4, 页码 1138-1149出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.10.059
关键词
Cell adhesion; Strain stiffening; Cell extensions; beta 1 Integrin
资金
- CIHR [STP-53877, MOP-418228]
- Canada Research Chair (Tier 1)
Cell adhesion responses to in-depth physical properties such as substrate roughness and topography are well described but little is known about the influence of lateral physical cues such as tissue boundaries on the function of adherent cells. Accordingly, we developed a model system to examine remote cell sensing of lateral boundaries. The model employs floating thin collagen gels supported by rigid grids of varying widths. The dynamics, lengths, and numbers of cell extensions were regulated by grid opening size, which in turn determined the distance of cells from rigid physical boundaries. In smaller grids (200 mu m and 500 mu m wide), cell-induced deformation fields extended to, and were resisted by, the grid boundaries. However, in larger grids (1700 mu m wide), the deformation field did not extend to the grid boundaries, which strongly affected the mean length and number of cell extensions (similar to 60% reduction). The generation of cell extensions in collagen gels required expression of the 01 integrin, focal adhesion kinase and actomyosin activity. We conclude that the presence of physical boundaries interrupts the process of cell-mediated collagen compaction and fiber alignment in the collagen matrix and enhances the formation of cell extensions. This new cell culture platform provides a geometry that more closely approximates the native basement membrane and will help to elucidate the roles of cell extensions and lateral mechanosensing on extracellular matrix remodeling by invasion and degradation. (C) 2013 Elsevier Ltd. All rights reserved.
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