Article
Chemistry, Applied
Xiaoli Qian, Hui Yu, Wenchao Zhu, Xufeng Yao, Wangwang Liu, Shikui Yang, Fangyuan Zhou, Yi Liu
Summary: The new fluorescence HOCl probe NFL-S combines high sensitivity and selectivity, successfully detecting endogenous HOCl and functioning in oxidative stress imaging.
Article
Chemistry, Analytical
Meng He, Miantai Ye, Bo Li, Tsunghsueh Wu, Chunfeng Lu, Pan Liu, Huiyue Li, Xingyu Zhou, Yanying Wang, Tao Liang, Haiyan Li, Chunya Li
Summary: In this study, a near-infrared fluorescent probe, TJM, was developed for selective and sensitive bioimaging of HClO. TJM exhibited excellent selectivity, high sensitivity, and low detection limit, making it suitable for imaging endogenous HClO in biological systems.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Applied
Guanwen Fan, Nannan Wang, Jian Zhang, Xin Ji, Shuchun Qin, Yuanfang Tao, Weili Zhao
Summary: This research developed a BODIPY-based probe for the selective and sensitive response of hypochlorous acid. The probe can detect hypochlorous acid at high concentrations quickly, and it can diagnose and visualize drug-induced liver injury.
Article
Chemistry, Multidisciplinary
Xu Jia, Chao Wei, Zimeng Li, Liyan Liu, Mei Wang, Pingzhu Zhang, Xiaoliu Li
Summary: A novel NIR hepatocyte-specific fluorescent probe named NHF was designed and synthesized with fast response and good selectivity to trace HClO. By using N-acetylgalactosamine (GalNAc) as the targeting ligand, NHF probe can be actively delivered to liver tissue, successfully visualizing and monitoring endogenous HClO in both cell models and animal tissues.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Chemistry, Analytical
Songjiao Li, Peipei Wang, Ke Yang, Ying Liu, Dan Cheng, Longwei He
Summary: In order to improve the prognosis and overall survival of hepatocellular carcinoma (HCC) patients, it is important to identify and resect it at an early stage. However, developing specific fluorescent probes to detect hypochlorous acid (HClO) in liver cells is still challenging. Therefore, the development of a new probe MBH-MT that can detect HClO in biological samples and image it in cells and tissues is of great significance for the diagnosis and treatment of HCC.
ANALYTICA CHIMICA ACTA
(2023)
Article
Spectroscopy
Huimin Shangguan, Qianwei Liu, Yuanjuan Wang, Zixuan Teng, Rumeng Tian, Tong Wu, Lei Yang, Ling Jiang, Xingjiang Liu, Liuhe Wei
Summary: In this study, a chromenoquinoline-based ratiometric fluorescent probe was developed for the detection and quantification of hypochlorous acid (HClO). The probe exhibited a large Stokes shift, high synthetic yield, simple synthesis method, short response time, low detection limit, and low toxicity. The probe was successfully validated in live cells and zebrafish.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Aishan Zheng, Han Liu, Chuanhao Peng, Xiaonan Gao, Kehua Xu, Bo Tang
Summary: CVS is a mitochondria-targeting near-Infrared fluorescent probe designed for sensing and detecting HOCl with high selectivity and low detection limit. It has been successfully applied to image HOCl in mitochondria of HepG2 cells, providing a perspective method for further study of physiological and pathological functions related to HOCl.
Article
Chemistry, Analytical
Zhuye Shang, Qingtao Meng, Run Zhang, Zhiqiang Zhang
Summary: This article reports the development of a bifunctional near-infrared fluorescent probe (QZB) that can selectively detect bisulfite and hypochlorous acid. The probe shows dual-function response, high sensitivity, and has been successfully applied in detecting these substances in real-world samples. The researchers have also demonstrated the probe's application in in vivo imaging.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Songjiao Li, Ke Yang, Peipei Wang, Ying Liu, Dan Cheng, Longwei He
Summary: Heat stroke is a devastating pathology with high morbidity and mortality rates. Understanding the cytotoxic mechanisms of heat can help prevent or treat stroke-related diseases. The role of lysosomes in heat stroke is poorly understood due to a lack of tools, but recent studies have shown a correlation between lysosome HClO and heat stroke development. To better interpret and treat heat stroke, a reliable real-time tool for tracking HClO levels is necessary, and MB-HClO, a near-infrared probe, shows promise in rapidly detecting lysosome HClO.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Peraya Hiranmartsuwan, Sirilak Wangngae, Jukkrit Nootem, Anyanee Kamkaew, Rathawat Daengngern, Worawat Wattanathana, Kantapat Chansaenpak
Summary: In this study, two pyridylhydrazone-tethered BODIPY compounds were synthesized and characterized. These compounds have the ability to selectively detect hypochlorous acid (HOCl) through a fluorescence turn-on mechanism. In cell imaging experiments, they showed excellent biocompatibility and visualization of endogenous HOCl in living cells.
Article
Chemistry, Applied
Guangshuai Zhou, Shaobo Hou, Ning Zhao, Nathaniel Finney, Yali Wang
Summary: A highly sensitive ratiometric fluorescent probe, NAP-RS, was designed for measuring fluctuations of lysosomal HOCl. The probe exhibited a clear fluorescence response to HOCl at biologically-relevant concentrations, with rapid response time and ultrasensitivity. Additionally, it could be used for visualizing exogenous and endogenous lysosomal HOCl.
Review
Chemistry, Inorganic & Nuclear
Lixuan Dai, Qian Zhang, Qingqing Ma, Weiying Lin
Summary: This review focuses on the application and development of dicyanoisophorone-based fluorescent probes. It covers the historical development, synthetic methods, optical properties, and sensing mechanisms of DCI fluorophores. It also discusses the design strategies and applications for detecting different analytes and provides future perspectives and challenges.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Wenjie Gao, Yanyan Ma, Yongyuan Liu, Shihan Ma, Weiying Lin
Summary: In this study, a near-infrared fluorescent probe CyClOP was developed for rapid detection of endogenous HClO, showing advantages of fast response, good selectivity, and deep penetration. The probe successfully captured the fluctuation of endogenous HClO in living cells during infection and injury conditions, providing a reliable tool for monitoring endogenous HClO and potential for future research in HClO-related biology and pathology.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Inorganic & Nuclear
Weijie Chen, Xiaoxie Ma, Huijuan Chen, Sheng Hua Liu, Jun Yin
Summary: Fluorescent probes play critical roles in monitoring the dynamic changes of pH and alkali metal ions, providing a portable means for real-time determination and clinical diagnosis. Over the recent years, numerous fluorescent probes have been developed and applied extensively in living systems.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Organic
Jiang He, Xiaoyong Chang, Chao Zou, Yanqin Yu, Shuang Han, Cuifang Wu, Sha Ou, Wei Lu, Kai Li
Summary: A series of compounds were prepared to achieve wide-tuning of absorption/emission wavelengths, with one selected compound showing satisfactory cytocompatibility and cytoplasm-targeting cell imaging ability.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xingxing Zhang, Feiyu Yang, Tianbing Ren, Yingxin Zheng, Xiao-Bing Zhang, Lin Yuan
Summary: In this study, two long-wavelength emission fluorophores with an optically tunable amino group were successfully developed for two-photon imaging. The fluorophores exhibited bright red emission, high two-photon action cross-section, and good water solubility, enabling higher signal-to-background ratios and deep tissue imaging. The probe was applied to the imaging of nitroreductase in liver fibrosis, showing potential for diagnosis of hypoxia during liver fibrosis.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Xiao-Peng Fan, Jing Huang, Tian-Bing Ren, Lin Yuan, Xiao-Bing Zhang
Summary: This study developed a novel near-infrared hemicyanines fluorophore (FJH) with good hydrophilicity and blood circulation, enabling lung imaging. The probe FJH-C showed selectivity and sensitivity towards the ALI indicator (esterase) in vitro and in living cells. This research provides a new method for real-time lung functional imaging and highlights the fluorophore structure with high lung retention.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yong Zhang, Wei Li, Xueqian Chen, Shaoqing Xiong, Yongning Bian, Lin Yuan, Xueyun Gao, Dongdong Su
Summary: Early diagnosis of hepatic inflammation is crucial for timely treatment and prevention of further liver damage. A NIRF/PA dual-modal probe (hCy-Tf-CA) has been developed for accurate early diagnosis of hepatic inflammation by integrating high sensitivity and spatial location feedback. The probe effectively detects superoxide anion fluctuation in vitro and achieves accurate in situ imaging of acute inflammatory liver injury and autoimmune hepatitis in vivo.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qian Liu, Jie Yuan, Renfeng Jiang, Longwei He, Xuefeng Yang, Lin Yuan, Dan Cheng
Summary: Gamma-Glutamyltransferase (GGT) is an important clinical biomarker associated with many diseases. A probe called NIR-GGT has been developed for imaging GGT activity. This probe can sensitively detect GGT and generate a strong fluorescent and photoacoustic signal. It can track changes in GGT level during early drug-induced acute liver injury (DIALI) and tumor resection with high temporal-spatial resolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lanlan Chen, Lin Yuan, Shan Zuo, Gangwei Jiang, Yingxin Zheng, Xingxing Zhang, Zuojia Qin, Tianbing Ren, Xiao-Bing Zhang
Summary: Bacterial infections can result from mishandled wounds or consumption of moldy food. Prompt diagnosis of bacterial infections is crucial to minimize anatomical damage, but current non-invasive early detection methods are challenging due to the lack of suitable tools. In this study, two hNQO1 fluorescent probes (RX2 and RX3) were developed through deep learning to visualize bacterial infections. RX2 and RX3 were found to enable early detection of bacterial infections using fluorescence imaging (FLI) and photoacoustic imaging (PAI) respectively, as demonstrated in a mouse model of myositis and inflammatory bowel disease caused by Escherichia coli infection. These probes show great potential for early diagnosis of bacterial infections, with RX2 suitable for FLI of E. coli-infected wounds and RX3 suitable for PAI of E. coli-infected inflammatory bowel disease.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Biomedical
Shuai Xu, Wenjing Pan, Lanlan Chen, Sulai Liu, Tian-Bing Ren, Hong-Wen Liu, Yongchao Liu, Shuang-Yan Huan, Lin Yuan, Xiao-Bing Zhang
Summary: Developing a chemiluminescence probe with slow kinetic profile is challenging but crucial for improving analytical sensitivity. A novel strategy was reported in this study to achieve long-lasting in vivo imaging by introducing hydrogen-bond-driven self-assembled dye HPQ to Schaap's dioxetane. The self-assembled chemiluminophore HPQCL-Cl showed prolonged half-life and constant emission, enabling long-lasting imaging and image-guided clinical surgery.
Review
Chemistry, Multidisciplinary
Qian Wu, Qian Lei, Hai-Chen Zhong, Tian-Bing Ren, Yao Sun, Xiao-Bing Zhang, Lin Yuan
Summary: Recently, the use of supramolecular chemistry to construct fluorescent systems based on macrocyclic hosts has gained significant attention. These systems have proven to be effective in overcoming the limitations of imaging and diagnostic reagents, and have been successful in enhancing their performances. This paper provides a summary of the recent advances in supramolecular fluorescent systems, their application in bioimaging and therapy, as well as the challenges and prospects in developing novel systems.
CHEMICAL COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Shuai Xu, Wenjing Pan, Zhi-Ling Song, Lin Yuan
Summary: Cell membrane is a phospholipid bilayer that maintains cell integrity and is involved in various physiological and pathological processes. There has been significant progress in developing small-molecular fluorescent probes for visualizing cell membrane-related events, especially in the near-infrared region. This review summarizes the design principles, targeting mechanisms, and biological applications of these probes and discusses the challenges and future directions in designing near-infrared cell membrane-targeting probes.
Review
Chemistry, Multidisciplinary
Lanlan Chen, Yifan Lyu, Xuan Zhang, Liting Zheng, Qingqing Li, Ding Ding, Fengming Chen, Yihao Liu, Wei Li, Yutong Zhang, Qiuling Huang, Zhiqiang Wang, Tiantian Xie, Qiang Zhang, Yingyu Sima, Ke Li, Shuai Xu, Tianbing Ren, Mengyi Xiong, Ying Wu, Jibin Song, Lin Yuan, Huanghao Yang, Xiao-Bing Zhang, Weihong Tan
Summary: Molecular imaging is a non-invasive method that allows the imaging and analysis of functional biomolecules in cells or in vivo at a molecular level. It plays a crucial role in understanding biological processes, diagnosing diseases early and accurately, and evaluating treatment effectiveness. This review summarizes the latest development of molecular imaging probes across various biomedical imaging modalities and discusses their design strategies, imaging modalities, properties, and applications in disease diagnosis, drug tracking, and imaging-guided surgery. The review also discusses the future perspectives and challenges in this emerging field, with the aim of inspiring the development of more effective molecular imaging probes for clinical practices.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Long He, Lin-Hui He, Huan-Hua Lai, Shuai Xu, Jiao-Liang Wang, Jun-Chao Xu, Ling Shi, Lin Yuan
Summary: We propose a practical strategy to design heavy-atom-free phototherapy agents (CSQs) with both PDT and PTT under NIR wavelength excitation by replacing the indole salt of xanthene Changsha (CS) with quinoline salt.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Haoming Chen, Ke Li, Lin Yuan, Xiao-Bing Zhang
Summary: In this study, an activatable near-infrared fluorescence (NIRF)/photoacoustic (PA) dual-mode imaging probe QHD-CE is presented for the detection of carboxylesterase (CE) in vitro and in vivo. QHD-CE exhibits excellent sensitivity and selectivity for CE with a high reaction efficiency (about 90 minutes). By using QHD-CE, the dynamic changes of CE in drug-induced liver injury and diabetic mouse models were monitored.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Xiaopeng Fan, Hongzhen Wang, Wen Yang, Tianbing Ren, Lin Yuan
Summary: A ratiometric photoacoustic probe responsive to HNO was developed to effectively assess HNO prodrug release and liver injury in vivo.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Zhe Li, Li Xu, Jin-Yu Li, Lingling Lei, Ping-Zhao Liang, Qian Wu, Feiyu Yang, Tian-Bing Ren, Xia Yin, Lin Yuan, Xiao-Bing Zhang
Summary: This study developed a water-soluble zwitterion with afterglow luminescence and renal metabolic capacity using a cascade reaction mediated by superoxide anion. Compared to other contrast agents, this zwitterion exhibited excellent afterglow properties, rapid renal metabolism, and good biocompatibility. It showed potential for the rapid detection of kidney failure with higher sensitivity and signal-to-background ratio in afterglow imaging.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Yuan Ye, Mengye He, Liping Wang, Xing-Can Shen, Hua Chen
Summary: This study developed a series of novel cyanine fluorophores for simultaneous visualization of cysteine level and mitochondrial viscosity during kidney ferroptosis. The results showed that ferroptosis process was accompanied by a decrease in cysteine channel and an increase in mitochondrial viscosity channel. Furthermore, the study also revealed dynamic changes of cysteine level and mitochondrial viscosity in a mouse kidney ferroptosis model in vivo.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yurong Feng, Shuai Xu, Haowei Guo, Tian-Bing Ren, Shuang-Yan Huan, Lin Yuan, Xiao-Bing Zhang
Summary: This study achieved sensitive and early diagnosis of acute kidney injury (AKI) through the development of a chemiluminescent probe. The probe showed high sensitivity in detecting urinary Vanin-1, an early biomarker for AKI. The study demonstrated the potential of the probe for early diagnosis of AKI and evaluating the efficiency of anti-AKI drugs.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.