Review
Biotechnology & Applied Microbiology
Jiaxue Liu, Boyan Jia, Zhibo Li, Wenliang Li
Summary: Applying reactive polymer materials sensitive to biological stimuli has attracted extensive research interest. The application of reactive oxygen species (ROS)-responsive polymers as drug-delivery systems in organisms has received much attention due to the special physiological effects of ROS on tumors or inflammation. The research progress of ROS-responsive polymers and their application in recent years were summarized and analyzed in this paper. From the perspective of nanoparticle drug delivery systems, multi-responsive delivery systems, and ROS-responsive hydrogels, the future development trends in this field were discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jia Liu, Lin Shi, Yang Wang, Mingyi Li, Cheng Zhou, Lifang Zhang, Chundong Yao, Ye Yuan, Daan Fu, Yan Deng, Miaodeng Liu, Guobin Wang, Lin Wang, Zheng Wang
Summary: A nanosized ruthenium-based metal-organic framework (Ru-MOF) with high porosity and versatile reactive oxygen and nitrogen species (RONS) scavenging activities has been developed for treating inflammatory diseases.
Article
Biochemistry & Molecular Biology
Li-Chiu Yang, Yu-Chao Chang, Kun-Lin Yeh, Fu-Mei Huang, Ni-Yu Su, Yu-Hsiang Kuan
Summary: This study investigated the protective mechanism of rutin in alleviating TEGDMA-induced toxicity in macrophages. Results showed that rutin substantially reduced cytotoxicity, apoptosis, and genotoxicity, and decreased oxidative stress and ROS generation caused by TEGDMA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Chloe Trayford, Darragh Crosbie, Timo Rademakers, Clemens van Blitterswijk, Rudy Nuijts, Stefano Ferrari, Pamela Habibovic, Vanessa LaPointe, Mor Dickman, Sabine van Rijt
Summary: Stem cell (SC)-based therapies have the potential to revolutionize therapeutics by enhancing the body's natural repair processes. Researchers have developed a new nanoparticle, AuMS, which can be used to trace and monitor the biodistribution and function of SCs in vivo, with the ability for multimodal imaging and simultaneous detection of reactive oxygen species (ROS).
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Physical
Chengwu Qiu, Yaroslav Odarchenko, Qingwei Meng, Shaojun Xu, Ines Lezcano-Gonzalez, Paul Olalde-Velasco, Francesco Maccherozzi, Laura Zanetti-Domingues, Marisa Martin-Fernandez, Andrew M. Beale
Summary: The presence of surface oxygen vacancies (O-vac) on TiO2 can effectively reduce Co3O4 nanoparticles to CoO/Co-0. O-vac are particularly effective at reducing the edges of the nanoparticles, with smaller particles being more easily reduced than larger ones. During heating in H-2/syngas, further reduction and consumption of O-vac prevent the total reoxidation of Co nanoparticles, especially the smallest particles, thus maintaining the presence of metallic Co and potentially enhancing catalyst performance.
Review
Environmental Sciences
Rekha Pachaiappan, Saravanan Rajendran, Pau Loke Show, Kovendhan Manavalan, Mu Naushad
Summary: Many microbial species causing infectious disease have become a social burden and threat to communities worldwide. The emergence of nanoparticles has shown potential in targeting and destroying microbes. Metal and metal oxide nanocomposites have gained interest for their antibacterial properties by damaging bacterial cell walls and leading to bacterial death.
Article
Engineering, Biomedical
Zhuangzhuang Zhang, Yang Pan, Ju-E Cun, Junhua Li, Zhaoyuan Guo, Qingqing Pan, Wenxia Gao, Yuji Pu, Kui Luo, Bin He
Summary: We developed a doxorubicin-incorporated iron coordination polymer nanoparticle (PCFD) for efficient chemo-chemodynamic cancer therapy by using a cinnamaldehyde-based reactive oxygen species (ROS)-replenishing organic ligand. This ligand can release cinnamaldehyde in response to ROS, supplementing intracellular H2O2 and depleting glutathione (GSH) through a thiol-Michael addition reaction. Together with doxorubicin-induced ROS upregulation and Fe3+-enabled GSH depletion, it facilitates efficient doxorubicin release and enhanced Fenton reaction, leading to redox dyshomeostasis and cancer cell death through concurrent apoptosis-ferroptosis. In vitro and in vivo studies show that the ROS-replenishing PCFD exhibits a better anticancer effect compared to the ROS-consuming counterpart. This study provides a facile and straightforward strategy for designing ROS amplifying nanoplatforms for cancer treatment.
ACTA BIOMATERIALIA
(2022)
Review
Chemistry, Inorganic & Nuclear
Nahyun Kwon, Dayeh Kim, K. M. K. Swamy, Juyoung Yoon
Summary: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play crucial roles in human life, but overproduction can lead to cell damage and diseases. Researchers have developed fluorescent and luminescent probes based on metal-coordinated systems to detect and image these active species, providing valuable tools for studying their roles in signaling and pathology.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Hyunjun Choi, Bongseo Choi, Jun-Hyeok Han, Ha Eun Shin, Wooram Park, Dong-Hyun Kim
Summary: A ROS-responsive cleavable hierarchical metallic supra-nanostructure (HMSN) is reported, which shows significant structural deformation in the presence of ROS and demonstrates potential in cancer treatment and other biomedical applications.
Article
Medicine, Research & Experimental
Qingxia Shen, Wanyi Huang, Yueyang Qiu, Shuze Wang, Bin Zhang, Ningning Sun, Qing Zhou
Summary: Bergapten (BGT) has been shown to alleviate osteoarthritis symptoms through its anti-inflammatory activity. This study demonstrates that BGT effectively reduces inflammation and improves chondrocyte response in a TMJOA rat model. Furthermore, BGT alters the intestinal flora and reduces ROS levels, providing a novel beneficial effect for the prevention and treatment of TMJOA.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Nanoscience & Nanotechnology
Santu Ghosh, Anupam Maity, Nikhil R. Jana
Summary: Piezoelectric nanomaterials can be used for water purification by utilizing ultrasound or water/air flow for degradation of toxic organics and bacterial disinfection through the generation of reactive oxygen species at the piezoelectric nanoparticle surface. However, the coupling of highly piezoelectric materials with appropriate water purification platforms is still not well explored. This study introduces a highly piezoelectric material-based composite coupled with a commercially available ultrasound platform for effective water purification, offering a promising alternative approach.
ACS APPLIED NANO MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Yiming Wang, Wenqi Luo, Feng Lin, Wanguo Liu, Rui Gu
Summary: EGCG-Se NP shows superior neuroprotective effect in spinal cord injury (SCI) through scavenging reactive oxygen species (ROS) and attenuating inflammation, making it a promising and effective treatment option.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Lorena M. Gomes, Daniele A. Moyses, Henrique F. S. Nascimento, Tatiane C. Mota, Lais T. Bonfim, Plinio C. S. Cardoso, Rommel M. R. Burbano, Marcelo O. Bahia
Summary: The study assessed the cytotoxic effects of dipyrone on Vero cells, showing significant genotoxicity and cytotoxicity, with a negative impact on cell viability.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2021)
Article
Plant Sciences
Indrani Manna, Saikat Sahoo, Maumita Bandyopadhyay
Summary: Researchers have found that nickel oxide nanoparticles affect the exposed roots of Allium cepa by disrupting cell membranes and being internalized within cell organelles, leading to disturbances in cellular homeostasis and survival. This external intervention causes changes in the cellular environment, reflected in crucial biochemical profiles.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Minyu Jing, Hanchao Zhang, Mingyue Wei, Yongwei Tang, Yan Xia, Yahua Chen, Zhenguo Shen, Chen Chen
Summary: DNA methylation is an important response strategy in plants under heavy metal stress. This study focused on the role of DNA methylation in plant adaptation to manganese and cadmium stress using pokeweed as a model. The results showed that different metals caused different response patterns in pokeweed, including subcellular distributions, ROS scavenging systems, transcriptional patterns, and DNA methylation. The number of differentially methylated loci increased with higher metal concentrations. Inhibition of NADPH oxidase reduced ROS levels and the expression of DNA methylase and demethylase. Furthermore, the study found that ROS played a role in mediating the changes in DNA methylation induced by manganese and cadmium stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Ning Gao, Stephen Mann
Summary: Complex coacervate microdroplets derived from associative phase separation have shown great potential in constructing membraneless protocells. However, the lack of an enclosing membrane limits their functionality. In this Account, we present chemical strategies for the membranization of coacervate microdroplets and discuss their potential applications as functional materials. We hope that this Account will inspire further research and development in this field.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Qi-Zhi Zhong, Joseph J. J. Richardson, Yuan Tian, Haijiang Tian, Jiwei Cui, Stephen Mann, Frank Caruso
Summary: Metal-quinone networks (MQNs) have been developed as structurally tunable, universally adhesive, hydrophilic, and pH-degradable materials by coordinating metal ions with quinones through metal-acetylacetone coordination bonds. MQNs exhibit bidirectional pH-responsive disassembly and can be assembled into various forms such as particles, tubes, capsules, and films. Leveraging the tunable release ability of MQNs and the medicinal properties of quinones, MQN prodrugs with high drug loading are engineered for anti-cancer therapy and inhibition of the main protease in the SARS-CoV-2 virus.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Ophthalmology
Iman Jalilian, Santoshi Muppala, Maryam Ali, Johnathon D. Anderson, Brett Phinney, Michelle Salemi, Phillip A. Wilmarth, Christopher J. Murphy, Sara M. Thomasy, VijayKrishna Raghunathan
Summary: This study investigates the effects of TGF-beta and ascorbic acid (AA) on the composition and stiffness of the extracellular matrix (ECM) secreted by corneal endothelial cells (CECs). It finds that TGF-beta decreases matrix stiffness while AA modulates the oxidative stress pathway in the matrix. These findings provide insights into the molecular mechanisms of Fuchs endothelial corneal dystrophy (FECD).
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Ophthalmology
A. L. Minella, M. I. Casanova, T. J. Chokshi, J. Kang, K. Cosert, M. M. Gragg, M. A. Bowman, M. E. Mccorkell, N. L. Daley, B. C. Leonard, C. J. Murphy, V. K. Raghunathan, S. M. Thomasy
Summary: This study aimed to assess the safety, effects, and efficacy of cysteamine hydrochloride (CH) as a TGM2 inhibitor on corneal wound healing and scarring. The results showed that CH had no adverse effects on corneal wound healing in a rabbit model and did not decrease corneal fibrosis.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Hannah Taylor, Ning Gao, Stephen Mann
Summary: We fabricate Janus-like calcium alginate hydrogel microspheres with spatially segregated populations using microfluidics. These microspheres serve as integrated platforms for domain-mediated chemical communication and programmable protocell-matrix dynamics. Our results offer new possibilities for the design and construction of spatially organized cytomimetic consortia capable of endogenous chemical processing and protocell-environment interactivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Editorial Material
Cell Biology
Natalia Jimenez-Moreno, Jon D. Lane
Summary: Parkinson's disease is caused by the loss of dopaminergic neurons in the substantia nigra pars compacta. In our recent study, we found that the transcription factors LMX1A and LMX1B play crucial roles in autophagy gene expression for stress protection in the developed brain. We propose a novel role for ATG8 proteins as transcriptional co-factors for autophagy genes in protecting dopaminergic neurons in Parkinson's disease.
Article
Cell Biology
Natalia Jimenez-Moreno, Madhu Kollareddy, Petros Stathakos, Joanna J. Moss, Zurine Anton, Deborah K. Shoemark, Richard B. Sessions, Ralph Witzgall, Maeve Caldwell, Jon D. Lane
Summary: LMX1A and LMX1B are essential for dopaminergic neuronal differentiation and survival, and they also act as transcription factors for cellular stress protection through autophagy. Their suppression impairs autophagy response and mitochondrial function, while their inducible overexpression protects against rotenone toxicity in vitro.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yanwen Zhang, Zefeng Wang, Mei Li, Can Xu, Ning Gao, Zhuping Yin, Kemin Wang, Stephen Mann, Jianbo Liu
Summary: The design and construction of synthetic protocells capable of stimuli response and homeostatic regulation is an important challenge for synthetic protobiology. In this study, we demonstrate a reconfigurable model protocell based on osmotic reconfiguration of lipid-coated coacervate droplets into multicompartmentalized coacervate vesicles that can exhibit hypotonic stress-induced volume response and enhance enzyme reactions. The increased nitric oxide (NO) within the swollen vesicles can induce blood vessel vasodilation, showing potential applications in biomedicine, cellular diagnostics, and bioengineering.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Multidisciplinary
Chengying Yin, Xingyu Jiang, Stephen Mann, Liangfei Tian, Bruce W. Drinkwater
Summary: The high throughput deposition of microscale objects with precise spatial arrangement is crucial in microfabrication technology. The utilization of dynamic acoustic fields offers a contactless approach to real-time reconfigurable miniaturized systems, with potential applications in various fields. This article discusses the physical interactions of microscale objects in an acoustic pressure field, the fabrication of acoustic trapping devices, methods to tune the spatial arrangement, and potential applications in different disciplines.
Editorial Material
Clinical Neurology
Lucy A. Crompton, Sarah F. McComish, Tom G. J. Steward, Daniel J. Whitcomb, Jon D. Lane, Maeve A. Caldwell
Summary: This scientific commentary discusses the article titled "Human stem cell-derived astrocytes exhibit region-specific heterogeneity in their secretory profiles" by Clarke et al. in Brain.
BRAIN COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Patrick J. Grimes, Mary Jenkinson-Finch, Henry E. Symons, Wuge H. Briscoe, Sebastien Rochat, Stephen Mann, Pierangelo Gobbo
Summary: The synthesis of a novel photocleavable, poly(ethylene) glycol-based crosslinker enables the realization of light-induced disassembly, pattern generation and membrane permeability modulation in protocells, opening up new avenues for the engineering of organized protocell networks and micro-compartmentalized systems for information storage and release.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Ophthalmology
VijayKrishna Raghunathan, Andrews Nartey, Kamesh Dhamodaran, Hasna Baidouri, Julia A. Staverosky, Kate E. Keller, Keith Zientek, Ashok Reddy, Ted Acott, Janice A. Vranka
Summary: Through studying the interaction between matrix and cells, we found that cells in different pressure and stiffening conditions have different responses. Although the mechanism of matrix stiffness cannot be directly determined, it is still an effective tool for studying cell-matrix interactions.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Zhuping Yin, Ning Gao, Can Xu, Mei Li, Stephen Mann
Summary: This article describes a self-driven, endosymbiotic-like pathway where proteinosomes are captured within membranized alginate/silk fibroin coacervate vesicles by guest-mediated reconfiguration. The interchange of coacervate vesicle and droplet morphologies through proteinosome-mediated activity produces discrete nested communities with integrated catalytic activity and selective disintegration.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Bret A. Moore, Iman Jalilian, Soohyun Kim, Makiko Mizutani, Madison Mukai, Connor Chang, Alec M. Entringer, Kamesh Dhamodaran, Vijay Krishna Raghunathan, Leandro B. C. Teixeira, Christopher J. Murphy, Sara M. Thomasy
Summary: The purpose of this study is to evaluate the elastic modulus, keratocyte-fibroblast-myocyte transformation, and haze formation of the corneal stroma following combined PTK and epithelium-off CXL using an in vivo rabbit model. The results showed that stromal haze formation and stiffness significantly increased following CXL, regardless of whether it is performed at or before the time of PTK. This study is important for understanding scar formation following CXL and for the development of improved therapeutic options.
Article
Ophthalmology
Brian C. Leonard, Sangwan Park, Soohyun Kim, Laura J. Young, Iman Jalilian, Krista Cosert, Xunzhi Zhang, Jessica M. Skeie, Hanna Shevalye, Nayeli Echeverria, Vanessa Rozo, Xin Gong, Chao Xing, Christopher J. Murphy, Mark A. Greiner, V. Vinod Mootha, Vijay Krishna Raghunathan, Sara M. Thomasy
Summary: This study examines the role of Wwtr1 in murine ocular structure and function, and its relationship with FECD. The results suggest that Wwtr1 deficient mice exhibit phenotypic abnormalities similar to FECD-affected patients, indicating that they could serve as a murine model for late-onset FECD.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.