Article
Engineering, Biomedical
Tomoyuki Kojima, Takashi Nakamura, Junichi Saito, Yuko Hidaka, Taisuke Akimoto, Hana Inoue, Christian Nanga Chick, Toyonobu Usuki, Makoto Kaneko, Etsuko Miyagi, Yoshihiro Ishikawa, Utako Yokoyama
Summary: Periodic hydrostatic pressurization under hypoxia enables the fabrication of scaffoldless human vascular mimetics with a spatial arrangement of cells and matrices. These mimetics show excellent mechanical properties and biocompatibility, making them suitable for clinical applications in cardiovascular diseases.
ACTA BIOMATERIALIA
(2023)
Review
Biochemistry & Molecular Biology
Taylor Chavez, Sharon Gerecht
Summary: Blood vessels play a crucial role in tissue development, functionality, and homeostasis, as well as in the healing and regeneration process. The microenvironment of tissues provides cues that affect cellular function, and engineering approaches can help us study and mimic different aspects of this microenvironment. This review focuses on the vascular niche, specifically the roles of oxygen and the extracellular matrix (ECM), and how vascular engineering approaches enhance our understanding of the microenvironment's impact on vascular regeneration. The limitations and future directions towards clinical utilization are also discussed.
TRENDS IN MOLECULAR MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Xiaochen Wang, Hangyu Li, Yu Zheng, Dongshi Guan, Aidan Wang, Qihui Fan, Yang Jiao, Fangfu Ye
Summary: The mechanical coupling between cells and extracellular matrix is crucial for the formation and dynamics of multicellular systems. By designing an in vitro experimental system, the authors found that the mechanical coupling between cells and ECM can induce cell aggregation and spreading behaviors, which are fundamentally different from cell monolayers. The reconstruction of collagen substrate into a dense fiber network structure enhances cell motility, while the weakening of fiber bundle formation within the hydrogel reduces cell movement. Moreover, cells can respond to the curvature and shape of the original cell population and form different aggregation patterns. These findings provide insight into the physical factors involved in collective cell organization and have implications for the development of biomaterials for new therapies and tissue engineering.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Multidisciplinary Sciences
Puja Jain, Rahul Rimal, Martin Moeller, Smriti Singh
Summary: The basement membrane physically separates the endothelial and epithelial monolayers of organs like lung, kidney, intestine, and eye. The topography of this matrix influences cell function and behavior. Mimicking these native features on an artificial scaffold system is important for replicating barrier function in vitro. This study investigates the influence of nano-scale topography on single cell behavior and monolayer formation. The results highlight the importance of scaffold topology in developing basement barrier function in in vitro models.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Biomedical
Jie Shi, Yanjiao Teng, Duo Li, Ju He, Adam C. Midgley, Xiaoqin Guo, Xiudan Wang, Xinran Yang, Shufang Wang, Yakai Feng, Qi Lv, Shike Hou
Summary: In this study, a tri-layered vascular graft was constructed using decellularized extracellular matrix (dECM) to mimic the defining features of native arteries. The results showed that dECM provided a bioactive environment for endothelial cell growth and migration. Additionally, it promoted smooth muscle regeneration and extracellular matrix deposition. This study provides a promising strategy for small-diameter vascular grafts.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Dylan Mostert, Bart Groenen, Leda Klouda, Robert Passier, Marie-Jose Goumans, Nicholas A. A. Kurniawan, Carlijn V. C. Bouten
Summary: In this study, the effect of ECM (dis)organization and cyclic strain on cardiomyocytes and cardiac fibroblasts (cFBs) was investigated in a two-dimensional human in vitro co-culture model of the myocardial microenvironment. The results showed that ECM protein patterning can guide cell orientation, and cFBs exhibit strain avoidance. However, cardiomyocytes only show strain avoidance when co-cultured with at least 30% cFBs via active remodeling of their sarcomeres.
APL BIOENGINEERING
(2022)
Article
Cell Biology
Betul Celebi-Saltik, Mustafa Ozgur Oteyaka, Beyza Gokcinar-Yagci
Summary: This study fabricated small-diameter vascular grafts using vascular tissue engineering, showing strong tensile strength and low platelet adhesion. The potential of these grafts for cardiovascular tissue repair is promising.
CONNECTIVE TISSUE RESEARCH
(2021)
Review
Chemistry, Multidisciplinary
Prerak Gupta, Biman B. Mandal
Summary: Vascular tissue engineering has made significant progress in recent years, with new technological advancements fostering the development of next-generation tissue-engineered vascular grafts. Traditional therapeutic methods involve bypassing damaged vessels with synthetic counterparts, while contemporary perspectives focus on biodegradable conduits with inherent remodeling capabilities.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Ryan Y. Nguyen, Aidan T. Cabral, Alejandro Rossello-Martinez, Alessandro Zulli, Xiangyu Gong, Qiuting Zhang, Jing Yan, Michael Mak
Summary: This study develops a collagen hydrogel that induces the formation of heterogeneous mesoscopic architectures, known as collagen islands. These island-containing gels have highly tunable inclusions and mechanical properties, with regional enrichment in collagen concentration at the cell-scale. The collagen island architectures are used to investigate mesenchymal stem cell behavior and demonstrate alterations in cell migration and osteogenic differentiation. Moreover, induced pluripotent stem cells cultured in island-containing gels show sufficient architecture to induce mesodermal differentiation. Overall, this work highlights the importance of complex mesoscopic tissue architectures as bioactive cues in regulating cell behavior and presents a novel collagen-based hydrogel for tissue engineering applications.
ADVANCED MATERIALS
(2023)
Article
Cell Biology
Meredith Whitehead, Syabira Yusoff, Sadia Ahmad, Lukas Schmidt, Manuel Mayr, Jillian Madine, David Middleton, Catherine M. Shanahan
Summary: Vascular amyloidosis, caused by the aggregation of peptide monomers into insoluble amyloid, is a common age-related pathology. This study found that extracellular vesicles (EVs) and HSPG2 in the extracellular matrix (ECM) play key roles in medin accumulation, contributing to the development of age-associated aortic medial amyloid (AMA).
Article
Engineering, Biomedical
Claire Robertson, Aimy Sebastian, Aubree Hinckley, Naiomy D. Rios-Arce, William F. Hynes, Skye A. Edwards, Wei He, Nicholas R. Hum, Elizabeth K. Wheeler, Gabriela G. Loots, Matthew A. Coleman, Monica L. Moya
Summary: Breast cancers have limited immune activation and expression of immunotherapy markers. The extracellular matrix may contribute to this limitation. Collagen 4 suppresses T cell function, while fibronectin activates T cells.
Review
Hematology
Prisca K. Lin, George E. Davis
Summary: Large and medium-sized vessels react differently from capillaries to stimuli inducing vascular disease, undergoing extracellular matrix (ECM) remodeling. While arteries persist and undergo modifications in response to injurious stimuli, capillaries may undergo regression responses.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Review
Peripheral Vascular Disease
Silvia Corvera, Javier Solivan-Rivera, Zinger Yang Loureiro
Summary: Adipose tissue is capable of massive expansion in adults, and its vasculature plays a crucial role in fuel supply and oxygenation. Impairments in angiogenic capacity may contribute to obesity-associated diseases. Recent studies have provided new insights into the mechanisms of adipose tissue angiogenesis and its impact on obesity.
Article
Biochemistry & Molecular Biology
Ghazi O. Bou Ghanem, Dmitry Koktysh, Robert O. Baratta, Brian J. Del Buono, Eric Schlumpf, Lauren K. Wareham, David J. Calkins
Summary: This study investigates the potential of collagen mimetic peptides (CMPs) in repairing damaged collagen in ocular tissues. The results demonstrate that CMPs can restore the stiffness and structure of collagen in optic nerve head tissues, offering a promising therapeutic avenue for ocular disorders involving collagen remodeling and degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Meng Zhang, Fenghua Zhao, Xue Zhang, Linda A. Brouwer, Janette K. Burgess, Martin C. Harmsen
Summary: This study found that MRC-5 fibroblasts promote collagen fiber remodeling and deposition of fibronectin in the hydrogel, leading to modifications in the ECM microenvironment and promoting vascularization.
MATERIALS TODAY BIO
(2023)
Article
Construction & Building Technology
Adrian Biggerstaff, Michael Lepech, Gerald Fuller, David Loftus
Summary: This paper proposes an elastic-plastic model to determine the final height of a 3D printed BSC layer, and validates the model using slump test experiments.
CONSTRUCTION AND BUILDING MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Milad Kamkar, Elnaz Erfanian, Parisa Bazazi, Ahmadreza Ghaffarkhah, Farbod Sharif, Ganhua Xie, Aadithya Kannan, Mohammad Arjmand, S. Hossein Hejazi, Thomas P. Russell, Gerald G. Fuller, Uttandaraman Sundararaj
Summary: The assembly of graphene oxide (GO) at oil/water or air/water interfaces significantly increases the elasticity of the interface, making it an exceptional candidate for stabilizing the O/W interface. This super elastic character is utilized for all-liquid 2D printing, highlighting the potential applications in electronics, fluidic devices, and controlled release systems.
ADVANCED MATERIALS INTERFACES
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Ngan F. Huang, Tatiana S. Zaitseva, Michael V. Paukshto
BIOENGINEERING-BASEL
(2023)
Article
Pharmacology & Pharmacy
John H. Klich, Catherine M. Kasse, Joseph L. Mann, Yaoqi Huang, Andrea D'Aquino, Abigail K. Grosskopf, Julie Baillet, Gerald G. Fuller, Eric A. Appel
Summary: This study demonstrates the ability of an amphiphilic copolymer excipient to enhance the stability of high concentration formulations of clinically relevant monoclonal antibodies (mAbs) without affecting their pharmacokinetics or injectability. The excipient competes with other components at the formulation interface, improving stability, and shows no significant impact on the monomeric composition or bioactivity of the antibodies. It has the potential to create stable, high concentration antibody formulations and enable improvements in treatment options.
ADVANCED THERAPEUTICS
(2023)
Article
Mechanics
Margaret Braunreuther, Maude Liegeois, John V. Fahy, Gerald G. Fuller
Summary: Programmable hydrogels, such as thiolated hydrogels, are widely used in tissue engineering and drug delivery applications due to their control over gelation, degradation, and adhesion. Understanding the mechanical properties of these materials during these processes is crucial for cell fate and delivery efficacy.
JOURNAL OF RHEOLOGY
(2023)
Article
Mechanics
Joung Sook Hong, Kyung Hyun Ahn, Gerald. G. Fuller, Min Chan Kim
Summary: This study investigates the evaporation-driven gravitational instabilities in the thin liquid film of a polymer solution during the drying process. The results show the scaling relationships between the onset of instability and the initial concentration for a slow evaporation rate and dilute concentration range, which rapidly increases with an increase in concentration. This study provides important information on the mechanism of convective instability in drying polymer solutions. English evaluation: 8 points
Article
Cardiac & Cardiovascular Systems
Ahmed Ibrahim Ahmed, Mahmoud Al Rifai, Fares Alahdab, Jean Michel Saad, Yushui Han, Moath Said Alfawara, Malek Nayfeh, Maan Malahfji, Faisal Nabi, John J. Mahmarian, John P. Cooke, William A. Zoghbi, Mouaz H. Al-Mallah
Summary: This study aimed to determine the effects of prior COVID-19 on coronary microvasculature. By considering factors such as time from infection, disease severity, SARS-CoV-2 variants, and subgroups of patients with diabetes and those with no known coronary artery disease, the study compared the myocardial flow reserve (MFR) between patients with previous COVID-19 and controls. The results showed that patients with prior COVID-19 had a significantly higher risk of impaired MFR.
EUROPEAN HEART JOURNAL-CARDIOVASCULAR IMAGING
(2023)
Article
Materials Science, Biomaterials
Philipp Baumli, Chunzi Liu, Aleksandar Bekcic, Gerald G. Fuller
Summary: The adhesive behavior of epithelial cell layers mediated by mucins plays a vital role in various biological processes. This study investigates the role of mucins in mediating axial epithelial adhesion and their impact on mechanical properties using ocular epithelial cell layers. The findings highlight the importance of mucins as soft cushions and nonlinear springs between cell layers, and suggest that changes in mucin levels can affect the adhesion between cells.
Article
Chemistry, Physical
Jan Zawala, Jonas Miguet, Preetika Rastogi, Omer Atasi, Mariusz Borkowski, Benoit Scheid, Gerald G. Fuller
Summary: This study provides the first quantitative proof of the influence of dynamic adsorption layers on the drainage dynamics of single foam films formed under dynamic conditions. By measuring the drainage dynamics of single foam films formed by air bubbles colliding against the interface between n-octanol solutions and air, it was found that the morphology of the single film during drainage is closely related to the state of the dynamic adsorption layer during the rising and bouncing steps of the bubble. It is demonstrated that the lifetime of surface bubbles is intimately related to the history of their formation.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Eleftheria Michalaki, Vinay N. Surya, Mariana Rodriguez-Hakim, Gerald G. Fuller, Alexander R. Dunn
Summary: This study demonstrates that the flow environment within lymphatic valves can activate key regulatory pathways involved in valve maintenance, as evidenced by the upregulation of gene transcription and calcium signaling in response to spatial and temporal variations in wall shear stresses.
Editorial Material
Mechanics
V. Chandran Suja, G. G. Fuller
Article
Engineering, Biomedical
David F. Chang, Karem A. Court, Rhonda Holgate, Elizabeth A. Davis, Katie A. Bush, Andrew P. Quick, Aldona J. Spiegel, Maham Rahimi, John P. Cooke, Biana Godin
Summary: Using autologous hSCS to treat deep skin wounds requires less donor skin and can prevent the decline of therapeutic effect caused by skin aging. Utilizing hTERT mRNA LNP can enhance the engraftment and proliferation of hSCS, reducing cellular senescence and DNA damage.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Physical
Kiara W. Cui, David J. Myung, Gerald G. Fuller
Summary: This study develops a tunable in vitro model of the mucin layer of the human tear film and demonstrates that higher mucin coverage can delay film breakup time by providing a thicker hydrated layer for stability. The model is compatible with interferometry for monitoring film thickness.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Multidisciplinary Sciences
Roderic I. Pettigrew, John P. Cooke
Summary: There has been a significant transformation in the scientific world, with accelerated discovery and innovation driven by interdisciplinary collaboration. The concept of Pasteur's quadrant, which combines fundamental understanding and application, has been expanded to include other quadrants contributing to scientific progress. The reimagined Pasteur's quadrant schema highlights the continuous fluid interchange among quadrants, leading to transformative advancements.
Article
Biochemical Research Methods
Suzanne G. K. Calhoun, Kara K. Brower, Vineeth Chandran Suja, Gaeun Kim, Ningning Wang, Alexandra L. McCully, Halim Kusumaatmaja, Gerald G. Fuller, Polly M. Fordyce
Summary: This article investigates the stability and size prediction of double emulsion droplets, providing a size parameterization model based on flow rate and buffer properties. The model is validated and shown to accurately predict droplet sizes. This research has the potential to simplify the development of novel screening-based biological assays using double emulsion droplets.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.