Article
Anatomy & Morphology
Caiyun Ma, Yang Liu, Yingchun Ma, Lijie Jiang, Qianyi Huang, Gaofeng Liu, Yu Guo, Chunjing Wang, Changqing Liu
Summary: This study successfully isolated PMSCs from Sprague-Dawley rat fetal lung tissue for the first time and demonstrated their stemness and multi-differentiation potentials, suggesting a potential regenerative cell resource for cell regenerative therapy of lung injury.
Review
Chemistry, Multidisciplinary
Yicen Long, Yujia Sun, Linlu Jin, Yixue Qin, Ye Zeng
Summary: Micropillars, a versatile microstructure, play a crucial role in bone repair by influencing key cellular processes in mesenchymal stem cells. This comprehensive review evaluates the impact of micropillars on cellular behavior and sheds light on potential regulatory mechanisms. Furthermore, micropillars are explored as a cutting-edge biomechanical detection tool and platform for investigating cellular behaviors.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Cell & Tissue Engineering
Mohamed Elashry, Mebrie Kinde, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: In this study, it was found that exposure to hypoxia enhanced the proliferation and motility of skeletal muscle-derived stem cells, promoted the differentiation of muscle and adipocytes, and improved the efficiency of myoblast differentiation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Environmental Sciences
Yifan Pan, Hui Qin, Lu Zheng, Yong Guo, Wei Liu
Summary: HFPO-TA and HFPO-DA have been shown to adversely affect human bone marrow mesenchymal stem cells, with effects on cell cycle processes and promotion of cell proliferation. They also inhibit osteogenic differentiation and increase the expression of core pluripotency regulator NANOG.
ENVIRONMENTAL POLLUTION
(2022)
Article
Nanoscience & Nanotechnology
Ke Deng, Ping Du, Kun Liu, Xuelian Tao, Javad Harati, Jhe-Wei Jhang, Jua Kim, Peng-Yuan Wang
Summary: This study successfully fabricated highly ordered structures with hybrid chemistry and stiffness using colloidal self-assembly technology, which significantly influenced the cell behavior and gene expressions of stem cells and macrophages in vitro.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Cell & Tissue Engineering
Jing-Yao Yin, Xing-Hong Luo, Wei-Qing Feng, Sheng-Hong Miao, Ting-Ting Ning, Qian Lei, Tao Jiang, Dan-Dan Ma
Summary: Tooth-related diseases and tooth loss are common public health issues, leading to the emergence of tooth regeneration science. Dental mesenchymal stem cells (DMSCs) play a significant role in tooth regeneration due to their undifferentiated state and multipotency, offering potential for reconstructing complete tooth tissues with normal functions and vascularization. Researchers aim to utilize the differentiation potential of DMSCs towards various tissue types to improve regenerative medicine and tissue engineering applications.
WORLD JOURNAL OF STEM CELLS
(2021)
Article
Chemistry, Multidisciplinary
Shakil N. N. Afraj, Chun-Hsiao Kuan, Jian-Sing Lin, Jen-Shyang Ni, Arulmozhi Velusamy, Ming-Chou Chen, Eric Wei-Guang Diau
Summary: Four X-shaped quinoxaline-based organic dyes, PQx (1), TQx, (2), PQxD (3), and TQxD (4), are developed as p-type self-assemble monolayer (SAM) for tin perovskite solar cells (TPSC). The thermal, optical, and electrochemical properties of these SAMs are thoroughly investigated. Tin perovskite layers are successfully deposited on these SAM surfaces using a two-step approach, and the achieved device performance follows the order of TQxD (8.3%) > TQx (8.0%) > PQxD (7.1%) > PQx (6.1%). Among them, TQxD (4), with a thiophene pi-extended conjugation unit in the SAM structure, exhibits the highest hole extraction rates, greatest hole mobilities, and slowest charge recombination, leading to the best reported efficiency of 8.3% for SAM-based TPSC. Moreover, all devices except PQx show good enduring stability, retaining approximately 90% of their original values after 1600 hours of shelf storage.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Multidisciplinary Sciences
Saeed Farjami, Karen Camargo Sosa, Jonathan H. P. Dawes, Robert N. Kelsh, Andrea Rocco
Summary: The article presents biologically motivated mathematical models for cell differentiation that generate oscillations, providing alternative explanations to Waddington's epigenetic landscape. Two generic dynamical scenarios for describing the differentiation process are proposed, involving gradual variation of control parameters. Analyses on repressilator and its variants indicate that gene regulatory networks with repressilator-like characteristics may play a role in cell fate selection through oscillatory processes.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2021)
Article
Engineering, Biomedical
Zachary T. Berent, Amy J. Wagoner Johnson
Summary: Experimental and simulation results on protein-micropatterned islands show a distinct morphological switch in cell proliferation that is better described as a function of island confluence rather than time in culture. Cell morphology, density, cell-cell contacts, and alkaline phosphatase expression are better predicted by confluence, indicating its importance in studying and interpreting cell behavior on different size and shape islands.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Anatomy & Morphology
Mohamed Elashry, Kateryna Gaertner, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The study compared the characteristics of MSCs from four different ovine muscles, revealing that hind limb and diaphragm muscle cells showed higher regenerative potential and myogenic differentiation compared to extraocular and masseter muscle cells. Extraocular and masseter muscle cells were more prone to adipogenic differentiation.
JOURNAL OF ANATOMY
(2021)
Article
Biotechnology & Applied Microbiology
Guanzhou Zhou, Ailing Tian, Xin Yi, Lufeng Fan, Wenchong Shao, Han Wu, Nianfeng Sun
Summary: The study demonstrated that Ad-MSCs from primary cultures exhibit stem cell characteristics, and self-assembled nanopeptide hydrogel is a good tissue engineering material. In the 3D-printed tissue model, Ad-MSCs grew well and retained strong differentiation ability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Liyang Ma, Qin Tang, Xin Gao, Joun Lee, Run Lei, Masako Suzuki, Deyou Zheng, Keisuke Ito, Paul S. Frenette, Meelad M. Dawlaty
Summary: Tet enzymes play a crucial role in hematopoiesis during embryogenesis by promoting the specification of HSPCs from endothelial cells. Their loss leads to reduced HSPC numbers and embryonic lethality.
Article
Biotechnology & Applied Microbiology
Hui Li, Zihao He, Wenjing Li, Jiao Jiao Li, Jianhao Lin, Dan Xing
Summary: Bone regeneration strategies based on MSC therapy have drawn widespread attention, but the effects of MSCs are variable. In this study, MSCs were incorporated into gelatin microcryogels to form microtissues and subjected to osteogenic induction. Undifferentiated and osteogenically induced microtissues mixed in a ratio of 2:1 showed the best outcomes of bone regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Yadi Wang, Wentao Dai, Zhixiao Liu, Jixiang Liu, Jie Cheng, Yuanyuan Li, Xueling Li, Jun Hu, Junhong Lu
Summary: A method based on single-cell FTIR microspectroscopy was developed to quantitatively evaluate cellular heterogeneity by calculating the cell-to-cell similarity distance, revealing that infrared phenotypes may reflect dynamic heterogeneity changes in cell populations. This provides an alternative label-free optical approach for quantifying cellular heterogeneity and can be combined with other single-cell analysis tools to understand the genotype-to-phenotype relationship in cellular populations.
ANALYTICAL CHEMISTRY
(2021)
Article
Energy & Fuels
Jie Hu, Chengliang He, Xiangjun Zheng, Yaokai Li, Xi Yang, Wenyuan Wang, Jie Zhang, Qi Chen, Fei Huang, Weifei Fu, Hongzheng Chen
Summary: Self-assembly monolayers (SAMs) of BnPA/F(5)BnPA mixture on ITO exhibit excellent interface properties, leading to high-performance PSCs based on PM6: BO-4Cl with a PCE of 18.0%. The improved energy level alignment, carrier-extraction ability, and reduced recombination contribute to the high performance of the device. The SAMs-modified ITO also shows superior stability compared to other modifications and comparable stability to PEDOT:PSS-based devices. The potential of SAMs for highly stable and high-performance PSCs is highlighted.
Review
Nanoscience & Nanotechnology
Stephanie Nemec, Kristopher A. Kilian
Summary: The dynamic epigenetic landscape directs gene expression patterns, drives cellular form, function, and tissue assembly, and influences cell plasticity in mammalian cells. Materials cues impact chromatin state through mechanotransduction, affecting cellular behavior and tissue activities.
NATURE REVIEWS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Sara Romanazzo, Thomas Gregory Molley, Stephanie Nemec, Kang Lin, Rakib Sheikh, John Justin Gooding, Boyang Wan, Qing Li, Kristopher Alan Kilian, Iman Roohani
Summary: This study demonstrates the creation of bone mimicking constructs using 3D printing of a novel apatite-transforming ink in a supportive microgel matrix with living cells, inspired by the bone biomineralization process. The technique allows for the fabrication of complex bone-mimicked constructs at room temperature without the need for invasive chemicals, radiation, or postprocessing steps. The mineralized constructs can be deposited within a high density of stem cells, directing the cellular organization and promoting osteogenesis in vitro, offering a new strategy for bone tissue regeneration.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Engineering, Biomedical
Yuwan Huang, Pavithra B. Jayathilaka, Md Shariful Islam, Carina B. Tanaka, Meredith N. Silberstein, Kristopher A. Kilian, Jamie J. Kruzic
Summary: This study evaluated the mechanical and biological properties of hybrid double network (DN) hydrogels and found that the monomer molecular weight and structure of polyethylene glycol (PEG) affected the mechanical properties. The alginate network played an important role in the DN hydrogel, and showed potential in guiding musculoskeletal differentiation. These findings are significant for the design of hydrogels with controllable properties.
ACTA BIOMATERIALIA
(2022)
Article
Materials Science, Multidisciplinary
Ayda Khosravanihaghighi, Pramod Koshy, Muhammad Yasir, Sara Romanazzo, Vedran Lovric, Kristopher Alan Kilian, Mark Duncan Willcox, William Robert Walsh, Charles Christopher Sorrell
Summary: This study provides a detailed interpretation of the role of Ga and Mn dopants, solid solubility mechanisms, charge compensation mechanisms, and antibacterial performance in CeO2 films. The results challenge the common assumption of the dominance of oxygen vacancies in ceria performance, showing that bioactivity is directly related to Ce3+ concentration. The antibacterial activity is influenced by the type, amount, and valence of the dopant, while cell proliferation is enhanced by doping at the lowest concentration.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Engineering, Biomedical
Thomas G. Molley, Tzong-tyng Hung, Kristopher A. Kilian
Summary: This study demonstrates how integrating live adipose derived stem cells with gradient microgel suspensions can steer divergent differentiation outcomes. The matrix formulation and size of microgels can be tuned to promote adipogenic or osteogenic differentiation. Freeform printing of high-density cell suspensions across these gradients allows for hierarchical tissue biofabrication.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Engineering, Biomedical
Gagan K. Jalandhra, Thomas G. Molley, Tzong-tyng Hung, Iman Roohani, Kristopher A. Kilian
Summary: This study developed a unique printing platform that integrates soft and hard materials concurrently through freeform printing of mineralized constructs within tunable micro-gel suspensions containing living cells. By tuning the microgel stiffness and filler content, chondrogenesis and osteogenesis can be differentially directed within the same construct, enabling the fabrication of osteochondral interfaces in a single step. This versatile one-pot biofabrication approach has the potential to aid in bone disease modeling and tissue engineering.
ACTA BIOMATERIALIA
(2023)
Article
Cell & Tissue Engineering
Sara Romanazzo, Chantal Kopecky, Shouyuan Jiang, Riddhesh Doshi, Vipul Mukund, Pallavi Srivastava, Jelena Rnjak-Kovacina, Kilian Kelly, Kristopher A. Kilian
Summary: This study demonstrates how matrix-conjugated hydrogel cell culture materials can normalize a population of induced pluripotent stem cell derived mesenchymal stem cells (iPSC-MSCs) to display a defined secretory profile that promotes enhanced neovascularization. Through a protein-conjugated biomaterials screen, two conditions were identified that promote reproducible secretion of pro-angiogenic and immunomodulatory cytokines. Furthermore, the study shows that secretory activity can be maintained after cryopreservation using defined culture substrates.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Engineering, Biomedical
Yiling Liu, Stephanie Nemec, Chantal Kopecky, Martina H. Stenzel, Kristopher A. Kilian
Summary: Nanoparticle drug formulations have advantages in cancer therapy, but the assessment of new drugs is limited by preclinical models. This study demonstrates a new approach to test nanomedicine using a microtumor array formed through hydrogel micropatterning. It allows simultaneous evaluation of drug uptake and efficacy in different cancer cell fractions with high reproducibility. The findings suggest that nanoparticle drug formulations are well suited for targeting the stem cell-like population in cancer therapy due to their relationship with drug resistance, recurrence, and metastasis.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Kochurani K. Johnson, Chantal Kopecky, Pramod Koshy, Yiling Liu, Michelle Devadason, Jeff Holst, Kristopher A. Kilian, Charles C. Sorrell
Summary: The time interval between tumor diagnosis and treatment initiation is crucial for survival rates. Theranostics, combining diagnosis and treatment, is expected to improve survival rates. This study reports the application of EGFR-functionalized ceria nanoparticles for EGFR-targeted diagnosis and treatment of melanoma. Evaluation of therapeutic efficiency was done using 2D and 3D models of melanoma, demonstrating the suitability of this nanoformulation for the treatment of metastatic melanoma.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Biology
Vina D. L. Putra, Kristopher A. Kilian, Melissa L. Knothe Tate
Summary: This review discusses the biomechanical, biophysical, and biochemical factors that regulate cytoskeletal remodeling during tissue neogenesis for targeted tissue regeneration. It highlights the effects of cytoskeletal remodeling on stem cell mechanoadaptation and lineage commitment. The review also summarizes findings on cytoskeletal remodeling in tissue neogenesis and its relevance in guiding tissue regeneration, as well as methods for visualizing and measuring cytoskeletal remodeling and its effects on cell mechanics.
COMMUNICATIONS BIOLOGY
(2023)
Article
Materials Science, Biomaterials
Panthipa Suwannakot, Stephanie Nemec, Newton Gil Peres, Eric Y. Du, Kristopher A. Kilian, Katharina Gaus, Maria Kavallaris, J. Justin Gooding
Summary: Synthetic hydrogels are widely used to mimic the extracellular matrix (ECM) and the physical and biochemical cues observed in natural ECM proteins. Researchers have developed an electrostatically crosslinked PEG-based hydrogel system to create high-throughput 3D in vitro models of the cancer environment. This hydrogel system can be degraded by breaking the interaction between oppositely charged polymer chains.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Matthew J. Moore, Yuen Ting Lam, Miguel Santos, Richard P. Tan, Nianji Yang, Juichien Hung, Zihao Li, Kristopher A. Kilian, Jelena Rnjak-Kovacina, Johannes B. Pitts, Henning Menzel, Steven G. Wise
Summary: Biomimetic scaffolds that mimic the architecture and biological activity of the extracellular matrix hold great potential for soft tissue engineering. In this study, we chemically modified chitosan, a natural polysaccharide, with synthetic polymer polycaprolactone (PCL) to create chitosan-graft-polycaprolactone (CS-g-PCL). These CS-g-PCL scaffolds showed improved mechanical strength and biological properties compared to pure PCL scaffolds, including enhanced blood compatibility, cell attachment and proliferation, and decreased inflammatory response.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Ashley K. Nguyen, Thomas G. Molley, Egi Kardia, Sylvia Ganda, Sudip Chakraborty, Sharon L. Wong, Juanfang Ruan, Bethany E. Yee, Jitendra Mata, Abhishek Vijayan, Naresh Kumar, Richard D. Tilley, Shafagh A. Waters, Kristopher A. Kilian
Summary: This article reports the discovery of a short peptide based on the tryptophan zipper motif, which shows multiscale hierarchical ordering and displays antimicrobial and self-healing properties. It can be used for cell delivery and bioprinting, and has important implications for supramolecular assembly of soft materials in biotechnology and medicine.
NATURE COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Stephanie Nemec, Sylvia Ganda, Karrar Al Taief, Chantal Kopecky, Rhiannon Kuchel, Helene Lebhar, Christopher P. Marquis, Pall Thordarson, Kristopher A. Kilian
Summary: This study demonstrates the production of a recombinant bacterial collagen-like protein (CLP) for thiol-ene pairing to norbornene functionalized hyaluronic acid (NorHA). The resulting hydrogel material shows adjustable modulus and strain-stiffening behavior similar to natural tumor matrices. It also exhibits good biocompatibility and can control cell adhesion, proliferation, and invasive properties of breast adenocarcinoma cells.
ACS APPLIED BIO MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Pavithra Bhakthi Jayathilaka, Thomas Gregory Molley, Yuwan Huang, Md Shariful Islam, Michael Robert Buche, Meredith Natania Silberstein, Jamie Joseph Kruzic, Kristopher Alan Kilian
Summary: Incorporating mechanosensitive linkages into polymers produces materials with dynamic force responsivity. The oxanorbornadiene cross-linked double network hydrogels release molecules through a force-mediated retro Diels-Alder reaction. Activation at physiologically relevant forces enables dynamic mechanochemical behavior in soft biological materials.
CHEMICAL COMMUNICATIONS
(2021)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
