Review
Biotechnology & Applied Microbiology
Liwei Zhu, Yuzhe Liu, Ao Wang, Zhengqing Zhu, Youbin Li, Chenyi Zhu, Zhenjia Che, Tengyue Liu, He Liu, Lanfeng Huang
Summary: Bone morphogenetic protein (BMP) plays an important role in promoting bone healing, but its mechanism needs further exploration. By modifying implants to carry BMP and achieve slow and sustained release, effective bone healing can be stimulated.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Jisun Park, Su Jeong Lee, Tae Gon Jung, Jun Hee Lee, Wan Doo Kim, Jae Young Lee, Su A. Park
Summary: Three-dimensional (3D) bioprinting allows for precise fabrication of scaffolds for tissue engineering, particularly those supporting cell attachment and osteogenic differentiation essential for bone tissue regeneration. Coating PCL scaffolds with PDA and depositing HA nanoparticles, followed by BMP-2 immobilization, promotes osteoblast proliferation and differentiation for potential bone tissue engineering applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Engineering, Biomedical
Xin Chen, Baoyu Tan, Zhiteng Bao, Shang Wang, Rongze Tang, Zhenmin Wang, Gaoyang Chen, Shuai Chen, William W. Lu, Dazhi Yang, Songlin Peng
Summary: The modification strategy using His6-T4L enhances the stability and solubility of BMP-2, allowing for controlled release and maximizing its activity to enhance bone regeneration. Additionally, the gelatin-based hydrogel incorporated with MPs-His6-T4L-BMP2 displays favorable properties for bone defect repair.
Article
Biotechnology & Applied Microbiology
Mustafa M. Kadhim, Dmitry Olegovich Bokov, Mohammad Javed Ansari, Wanich Suksatan, Mohammed Abed Jawad, Supat Chupradit, Mohammed N. Fenjan, Milad Kazemnejadi
Summary: In this study, blend nanofibrous scaffolds were electrospun using polycaprolactone/gelatin blend solutions reinforced by BMP-modified graphene oxide. The resulting nanofibers exhibited uniform and bead-less morphology with improved mechanical and physical properties due to the addition of PCL and GO. The incorporation of BMP-modified graphene in the blend led to the formation of nanofibrous substrate resembling bone tissue, which showed good biocompatibility with human osteosarcoma cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Engineering, Biomedical
M. Bouyer, C. Garot, P. Machillot, J. Vollaire, V Fitzpatrick, S. Morand, J. Boutonnat, V Josserand, G. Bettega, C. Picart
Summary: The study suggests that using 3D-printed PLA scaffolds loaded with reduced doses of BMP-2 may be a safe and simple solution for large bone defects in the clinic. The dose of BMP-2 delivered from the scaffold significantly influences the amount of regenerated bone and the repair kinetics, showing good bone repair outcomes.
MATERIALS TODAY BIO
(2021)
Article
Biochemistry & Molecular Biology
Ruonan Zhang, Jun-Ichiro Jo, Ryuhei Kanda, Aki Nishiura, Yoshiya Hashimoto, Naoyuki Matsumoto
Summary: The study modified the surface of polyetheretherketone (PEEK) with sandblasting and polydopamine (PDA) to incorporate a gelatin hydrogel, enabling sustained release of growth factors and osteogenic differentiation of stem cells. The success of gelatin hydrogel binding and degradation with PEEK (GPEEK) was confirmed, along with mineralization in simulated body fluid. The sustained release of bone morphogenetic protein (BMP)-2 in GPEEK led to osteogenic differentiation of human mesenchymal stem cells (hMSCs). PEEK with a gelatin hydrogel incorporating BMP-2 shows promise as a substrate for bone tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Benjamin Tajer, James A. Dutko, Shawn C. Little, Mary C. Mullins
Summary: The study explores the BMP2/7 heterodimer signaling mechanism in zebrafish embryos, revealing that BMP2 homodimers require nonredundant type I receptors Acvr1 and Bmpr1 for signaling, despite not being able to bind Acvr1 in vitro. The kinase activity of Acvr1 is essential for the signaling complex, suggesting that the potency of the heterodimer arises from recruiting both Acvr1 and Bmpr1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Xianggang Wang, Zhengyan Li, Zhonghan Wang, He Liu, Yutao Cui, Yuzhe Liu, Ming Ren, Hongsheng Zhan, Zuhao Li, Minfei Wu, Jincheng Wang
Summary: The study developed a thermosensitive hydrogel loaded with BMP-2 and OPG to promote bone formation and inhibit osteoclast activity, which was then used to construct 3D-printed composite scaffolds. These scaffolds facilitated osteogenic differentiation, reduced osteoclastic activation, and improved bone ingrowth and osseointegration through sustained release of BMP-2 and OPG.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Jan Henkel, Flavia Medeiros Savi, Arne Berner, Stephanie Fountain, Siamak Saifzadeh, Roland Steck, Devakar R. Epari, Maria A. Woodruff, Mark Knackstedt, Michael A. Schuetz, Dietmar W. Hutmacher
Summary: This study presents a newly established preclinical ovine animal model for treating large volume segmental tibial defects. It utilizes mPCL-TCP scaffolds combined with rhBMP-7 and PRP for treatment, with detailed analysis on mechanical properties of the scaffolds, IFM, and DCP-surface strain.
Article
Biochemistry & Molecular Biology
Johannes Bulk, Valentyn Kyrychenko, Philipp M. Rensinghoff, Zahra Ghaderi Ardekani, Stephan Heermann
Summary: Vision is dependent on the correct development of the eye, which is tightly connected to the development of the forebrain. Holoprosencephaly (HPE) is a developmental disorder where the eye field and the forebrain fail to separate, leading to ocular abnormalities such as cyclopia, anophthalmia, and coloboma. This study identifies the importance of a balance between Bone morphogenetic proteins (BMPs) and their antagonists in eye and forebrain cleavage, and demonstrates that dysregulation of BMP signaling results in severe forms of HPE with anophthalmia. Additionally, the study suggests that BMP signaling also affects the development of hypothalamic cells during neurulation and neural keel formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yoshiaki Soejima, Nahoko Iwata, Ran Nishioka, Mako Honda, Yasuhiro Nakano, Koichiro Yamamoto, Atsuhito Suyama, Fumio Otsuka
Summary: Orexins play important roles in sleep-wake regulation and food intake in the central nervous system. This study investigated the functions of orexin in adrenal steroidogenesis and its interaction with bone morphogenetic proteins (BMPs). The results suggest that both orexin and BMP-6 accelerate adrenocortical steroidogenesis in human adrenocortical cells, and these pathways mutually inhibit each other.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yoshiaki Soejima, Nahoko Iwata, Nanako Nakayama, Shinichi Hirata, Yasuhiro Nakano, Koichiro Yamamoto, Atsuhito Suyama, Kohei Oguni, Takahiro Nada, Satoshi Fujisawa, Fumio Otsuka
Summary: Orexin plays a key role in regulating sleep, wakefulness and feeding behavior. This study shows that Orexin regulates gonadotropin expression by interacting with GnRH and the pituitary BMP system, and this regulation involves clock gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Neda Zakeri, Hamid Reza Rezaie, Jafar Javadpour, Mahshid Kharaziha
Summary: A novel polycaprolactone-zeolite nanocomposite scaffold was developed for sustained release of cisplatin in bone cancer treatment. The scaffold exhibited high porosity and suitable pore diameters, and the release of cisplatin had a cytotoxic effect on MG63 cells through a pH-sensitive and sustained release mechanism.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2022)
Article
Biochemistry & Molecular Biology
Fabien Kawecki, Jessica Jann, Michel Fortin, Francois A. Auger, Nathalie Faucheux, Julie Fradette
Summary: The success of dental implant treatment after tooth extraction can be improved by preserving the alveolar ridge using cell-free biomaterials. The use of a bone-like substitute supplemented with bone morphogenetic protein (BMP) can enhance in vitro osteogenic potential and promote alveolar bone healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Polymer Science
Xiangjun Yang, Yuting Wang, Ying Zhou, Junyu Chen, Qianbing Wan
Summary: PCL is widely used in bone tissue engineering for its good biocompatibility and slow degradation rate, and can be blended with other polymers and hydrogels to improve its properties. 3D printing techniques are utilized to fabricate PCL-based composite scaffolds for various clinical applications.
Review
Chemistry, Multidisciplinary
Barbara Schamberger, Ricardo Ziege, Karine Anselme, Martine Ben Amar, Michal Bykowski, Andre P. G. Castro, Amaia Cipitria, Rhoslyn A. Coles, Rumiana Dimova, Michaela Eder, Sebastian Ehrig, Luis M. Escudero, Myfanwy E. Evans, Paulo R. Fernandes, Peter Fratzl, Liesbet Geris, Notburga Gierlinger, Edouard Hannezo, Ales Iglic, Jacob J. K. Kirkensgaard, Philip Kollmannsberger, Lucja Kowalewska, Nicholas A. Kurniawan, Ioannis Papantoniou, Laurent Pieuchot, Tiago H. V. Pires, Lars D. Renner, Andrew O. Sageman-Furnas, Gerd E. Schroder-Turk, Anupam Sengupta, Vikas R. Sharma, Antonio Tagua, Caterina Tomba, Xavier Trepat, Sarah L. Waters, Edwina F. Yeo, Andreas Roschger, Cecile M. Bidan, John W. C. Dunlop
Summary: Surface curvature plays an important role in biological systems, from cell membranes to tissues and organs. Experimental and theoretical investigations have supported the relevance of surface curvature in biology. This review provides an introduction to the key concepts of surface curvature in biological systems and discusses the challenges in measuring it. The review also highlights the response of cells, tissues, and organisms to curvature, as well as the interplay between the distribution of morphogens or micro-organisms and the emergence of curvature.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Martin Textor, Arnd Hoburg, Rex Lehnigk, Carsten Perka, Georg N. N. Duda, Simon Reinke, Antje Blankenstein, Sarah Hochmann, Andreas Stockinger, Herbert Resch, Martin Wolf, Dirk Strunk, Sven Geissler
Summary: This study found that loose bodies (LBs) from patients with osteochondritis dissecans (OCD) contain sufficient viable and functional chondrocytes that can be used for autologous chondrocyte implantation (ACI) without affecting their phenotype during prolonged in vitro expansion. The chondrocyte populations from LBs (LB-CH) showed no significant differences in cell yield per isolation and expression of chondrocyte progenitor cell markers compared to control chondrocytes from non-weight-bearing joint regions. LB-CH exhibited comparable viability, proliferation rates, and chondrogenic differentiation potential to control chondrocytes, and had a higher ability to form a hyaline cartilage matrix compared to bone marrow mesenchymal stromal cells (BMSCs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
Alexander Paping, Clara Basler, Kerstin Melchior, Loreen Ehrlich, Mario Thiele, Georg N. Duda, Wolfgang Henrich, Thorsten Braun
Summary: The study aimed to evaluate the reliability of intraoperative ultrasound in identifying histologically confirmed uterine scar tissue. The results showed that intraoperative ultrasound was more accurate in identifying scar tissue compared to preoperative ultrasound. This is important for studying uterine wound healing and preventing uterine wall rupture.
JOURNAL OF PERINATAL MEDICINE
(2023)
Review
Cardiac & Cardiovascular Systems
Maurizio Pesce, Georg N. Duda, Giancarlo Forte, Henrique Girao, Angel Raya, Pere Roca-Cusachs, Joost P. G. Sluijter, Carsten Tschope, Sophie Van Linthout
Summary: A growing body of evidence suggests that the mechanical functions of cardiac fibroblasts, as a necessary component, play an active role in myocardial growth and homeostasis. This review describes cell mechanosensation as a regulator of cardiac maturation and disease, and provides evidence that changes in the mechanical properties of the myocardium can occur due to disease-induced remodeling of the cardiac extracellular matrix.
NATURE REVIEWS CARDIOLOGY
(2023)
Article
Engineering, Biomedical
Agathe Bessot, Jennifer Gunter, David Waugh, Judith A. Clements, Dietmar W. Hutmacher, Jacqui McGovern, Nathalie Bock
Summary: Increasing evidence highlights the important role of bone marrow adipocytes in prostate cancer bone metastases. However, lack of relevant models has hindered the understanding of human BM-adipocyte effects in this microenvironment. This study combines tunable GelMA-based hydrogels with biomimetic culture of human cells to engineer human bone tumor microenvironments containing BM-adipocytes. In vitro culture of human osteoprogenitors, adipocytes, and PCa cells in GelMA hydrogels leads to the formation of mineralized, adipose, and PCa tumor 3D microtissues, respectively. The stiffness of hydrogels regulates osteoblast mineralization and tumor spheroid formation, while coculture with tumor cells induces morphological changes and delipidation in BM-adipocytes. The in vivo model successfully forms a humanized fatty bone microenvironment and demonstrates that adipocytes support early bone tumor growth. This advanced platform combines natural aspects of the microenvironment with tunable properties for bone tumor research.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Sport Sciences
Nicholas M. Brisson, Leonie A. N. Krahl, Martin Kraemer, Juergen R. Reichenbach, George N. Duda
Summary: This study assessed the effects of the COVID-19 pandemic restrictions on physical activity levels, body mass, quadriceps strength, and gait biomechanics over 18 months. The results showed that participants had reduced physical activity levels, increased body weight, a tendency toward reduced quadriceps strength, and altered gait biomechanics during the lockdown period.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2023)
Article
Cell Biology
Sarah Hochmann, Kristy Ou, Rodolphe Poupardin, Michaela Mittermeir, Martin Textor, Salaheddine Ali, Martin Wolf, Agnes Ellinghaus, Dorit Jacobi, Juri A. J. Elmiger, Samantha Donsante, Mara Riminucci, Richard Schaefer, Uwe Kornak, Oliver Klein, Katharina Schallmoser, Katharina Schmidt-Bleek, Georg N. Duda, Julia K. Polansky, Sven Geissler, Dirk Strunk
Summary: Multipotent stromal cells are attractive sources for cell therapy and tissue engineering, but their application is limited due to functional diversity. In this study, we compared the regenerative potential of different stromal cell types in vitro and in vivo and found that only bone marrow stromal cells (BMSCs) could fully regenerate critical-size femoral defects. This ability was attributed to cell type-specific epigenetic landscapes controlling transcriptional stromal differentiation networks. BMSCs initiated defect healing and eventually contributed to tissue remodeling.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
David S. Sparks, Flavia M. Savi, Constantin E. Dlaska, Siamak Saifzadeh, Gary Brierly, Edward Ren, Amaia Cipitria, Johannes C. Reichert, Marie-Luise Wille, Michael A. Schuetz, Nicola Ward, Michael Wagels, Dietmar W. Hutmacher
Summary: A preclinical evaluation was conducted on eight sheep with a tibial critical-size segmental bone defect using a regenerative medicine methodology. The evaluation showed successful bone regeneration using the RMAV approach with a mPCL-TCP scaffold, which was superior to a scaffold control group. This study demonstrates the potential of bench-to-bedside research in reconstructive surgery and regenerative medicine.
Article
Endocrinology & Metabolism
Jan Barcik, Manuela Ernst, Tim Buchholz, Caroline Constant, Karen Mys, Devakara R. Epari, Stephan Zeiter, Markus Windolf
Summary: This study compares the effects of immediate and delayed application of mechanical stimulation on fracture healing in a large animal model. The results show that immediate stimulation promotes fracture callus development, while delayed stimulation slows down the healing progress.
Article
Materials Science, Biomaterials
Claudia A. Garrido, Daniela S. Garske, Mario Thiele, Shahrouz Amini, Samik Real, Georg N. Duda, Katharina Schmidt-Bleek, Amaia Cipitria
Summary: This article introduces a patterned biomaterial based on alginate hydrogels that can mimic the biophysical and biochemical properties found in nature. By controlling the patterns of stiffness and degradation in the material, the cell-matrix interactions can be studied in great detail. The results show that the cell response to the material patterns can be quantified using image-based strategies, providing a deeper understanding of cell-matrix interactions in multicomponent materials.
BIOMATERIALS ADVANCES
(2023)
Editorial Material
Cell Biology
Georg N. Duda, David W. Grainger, Robert E. Guldberg, Gady Goldsobel, Glenn D. Prestwich, Brenna Rauw, Hans-Dieter Volk
Summary: Translational impact assessment is crucial in determining the potential of biomedical research discoveries to be transformed into practical, beneficial products for human health. However, measuring translational success is challenging as it goes beyond commercial achievements and may vary from case to case. Prioritizing societal impact is essential in a globalized society.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Mariya M. Kucherenko, Pengchao Sang, Juquan Yao, Tara Gransar, Saphala Dhital, Jana Grune, Szandor Simmons, Laura Michalick, Dag Wulsten, Mario Thiele, Orr Shomroni, Felix Hennig, Ruhi Yeter, Natalia Solowjowa, Gabriela Salinas, Georg N. Duda, Volkmar Falk, Naren R. Vyavahare, Wolfgang M. Kuebler, Christoph Knosalla
Summary: Pulmonary hypertension worsens prognosis in left heart disease. Increased stiffness of the pulmonary artery may contribute to this by causing right ventricular dysfunction and lung vascular remodeling. Targeting extracellular matrix remodeling may be a therapeutic approach for pulmonary hypertension in left heart disease.
NATURE COMMUNICATIONS
(2023)
Review
Engineering, Biomedical
Markus Laubach, Frank Hildebrand, Sinduja Suresh, Michael Wagels, Philipp Kobbe, Fabian Gilbert, Ulrich Kneser, Boris M. Holzapfel, Dietmar W. Hutmacher
Summary: The treatment of bone defects is a challenging clinical problem with high reintervention rates and significant healthcare costs. Three-dimensional printing has revolutionized the treatment of bone defects by enabling personalized therapies with highly porous 3D-printed implants. However, there are still challenges to be addressed, including the development of guidelines, clinical trial design, and financing support.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Mairim Russo Serafini, Alexandra Mowat, Susanah Mustafa, Siamak Saifzadeh, Tara Shabab, Onur Bas, Nicholas O'Rourke, Dietmar W. Hutmacher, Flavia Medeiros Savi
Summary: This study evaluated the use of mPCL scaffolds loaded with PRP for hernia repair in sheep models, and found that the mPCL scaffolds enhanced soft tissue regeneration. After 6 months, the mPCL scaffolds showed similar mechanical properties, collagen, and vascular ingrowth compared to PP meshes. This pilot study supports the use of mPCL as a safe and effective biodegradable scaffold for hernia and vaginal prolapse repair.
BIOENGINEERING-BASEL
(2023)