期刊
BIOMATERIALS
卷 34, 期 3, 页码 713-723出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.10.027
关键词
Partial-thickness cartilage defects; Spontaneous repair; Mesenchymal stem cells; Matrix environment; Type 1 collagen scaffold; Stromal cell-derived factors-1
资金
- National Basic Research Program of China (973 Program) [2012CB966604]
- National Natural Science Foundation of China [81125014, 81071461, J1103603]
- Zhejiang Provincial Natural Science Foundation [Z2100086]
- National High Technology Research and Development Program of China [2012AA020503]
Despite the presence of cartilage-derived mesenchymal stem cells (C-MSCs) and synovial membrane-derived mesenchymal stem cells (SM-MSCs) populations, partial-thickness cartilage defects, in contrast to the full-thickness defects, are devoid of spontaneous repair capacity. This study aims to create an in situ matrix environment conducive to C-MSCs and SM-MSCs to promote cartilage self-repair. Spontaneous repair with MSCs migration into the defect area was observed in full-thickness defects, but not in partial-thickness defects in rabbit model. Ex vivo and in vitro studies showed that subchondral bone or type 1 collagen (coil) scaffold was more permissive for MSCs adhesion than cartilage or type 2 collagen (col2) scaffold and induced robust stromal cell-derived factors-1 (SDF-1) dependent migration of MSCs. Furthermore, creating a matrix environment with coil scaffold containing SDF-1 enhanced in situ self-repair of partial-thickness defects in rabbit 6 weeks post-injury. Hence, the inferior self-repair capacity in partial-thickness defects is partially owing to the non-permissive matrix environment. Creating an in situ matrix environment conducive to C-MSCs and SM-MSCs migration and adhesion with coil scaffold containing SDF-1 can be exploited to improve self-repair capacity of cartilage. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据