Article
Multidisciplinary Sciences
Zhilun Zhao, Chen Chen, Shixuan Wei, Hanqing Xiong, Fanghao Hu, Yupeng Miao, Tianwei Jin, Wei Min
Summary: Raman-active nanoparticles (Rdots) with ultra-brightness and compact sizes are developed for high-resolution imaging of complex biological networks and immunostaining of specific protein targets.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Maha K. Rahim, Jinghui Zhao, Hinesh V. Patel, Hauna A. Lagouros, Rajesh Kota, Irma Fernandez, Enrico Gratton, Jered B. Haun
Summary: Fluorescence lifetime-based multiplexing allows simultaneous detection of multiple probes, and the phasor approach provides a simple solution for lifetime analysis. This study demonstrates the ability of phasor analysis to resolve and quantify exogenous fluorescent probes based on lifetime information alone. It also shows that lifetime-based quantitation accuracy can be improved through intensity matching. The results highlight the potential of fluorescence lifetime imaging and phasor analysis as a powerful tool for simultaneous detection of multiple biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Xiaobo Zhou, Lingfeng Zhao, Ke Zhang, Chaojie Yang, Shijie Li, Xiaoxia Kang, Guo Li, Qi Wang, Haiwei Ji, Mingmin Wu, Jinxia Liu, Yuling Qin, Li Wu
Summary: This study presented a strategy for designing and fabricating narrow emissive AIEdots with tunable wavelengths by constructing a light-harvesting system with high energy transfer efficiency, resulting in high brightness and narrow emission.
Article
Chemistry, Multidisciplinary
Alexander M. Saeboe, Alexey Y. Nikiforov, Reyhaneh Toufanian, Joshua C. Kays, Margaret Chern, J. Paolo Casas, Keyi Han, Andrei Piryatinski, Dennis Jones, Allison M. Dennis
Summary: This report showcases the reddest emitting indium phosphide quantum dots to date, demonstrating tunable near-infrared photoluminescence and PL multiplexing in the first optical tissue window while avoiding toxic constituents. By utilizing an inverted QD heterostructure, the synthesis overcomes the growth bottleneck and extends the emission peak of the nanoparticles into the deeper optical tissue window.
Article
Chemistry, Analytical
Sahil S. Kanani, Hsin-Yun Tsai, W. Russ Algar
Summary: This study reports a double-chopper design for time-gated photoluminescence (PL) imaging using a portable, 3D-printed, smartphone-based device. The device is capable of quantitative imaging of PL, including spectral and temporal multiplexing, TG-FRET assay, and cell imaging. This work is the first demonstration of these important analytical capabilities on a smartphone, providing an important foundation for developing smartphone-based POCD methods using time-gated PL imaging.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Sahil S. Kanani, Hsin-Yun Tsai, W. Russ Algar
Summary: This study presents a double-chopper design for time-gated photoluminescence imaging using a portable, 3D-printed, smartphone-based device. The device enables quantitative imaging and rejection of background fluorescence, making it useful for studying luminescent lanthanide complexes and performing spectral and temporal multiplexing. These findings lay an important foundation for developing point-of-care diagnostics methods based on time-gated photoluminescence imaging.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Philipp Kunze, Lucas Kreiss, Vendula Novosadova, Adriana V. Roehe, Sara Steinmann, Jan Prochazka, Carol I. Geppert, Arndt Hartmann, Sebastian Schuermann, Oliver Friedrich, Regine Schneider-Stock
Summary: In this study, the ECM remodeling potential of colorectal tumor cells was investigated using multiphoton microscopy and the chorioallantoic membrane assay. The results showed that DAPK1 ko tumor cells have a high ECM remodeling potential at the tissue level.
Article
Biochemistry & Molecular Biology
Janina Burk, Anna Sassmann, Cornelia Kasper, Ariane Nimptsch, Susanna Schubert
Summary: Matrix remodeling by MSC in ECM disease could be influenced by the ECM environment. MSC adapt to the ECM conditions, including pathological adaptations, which may affect therapeutic efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Huaqing Jing, Yingzi Ren, Yue Zhou, Min Xu, Sona Krizkova, Zbynek Heger, Qiang Lu, Siyu Wang, Xiaoyang Liang, Vojtech Adam, Nan Li
Summary: This study developed a new method for treating liver fibrosis by using nanoenzymes loaded with nilotinib to inhibit the activation of hepatic stellate cells and improve the microenvironment of liver fibrosis. It was found that these nanoenzymes effectively eliminated intrahepatic reactive oxygen species, alleviated the hypoxic microenvironment, and promoted collagen depletion. In mouse experiments, the nanoenzymes showed significant antifibrogenic effects without obvious long-term toxicity.
ACTA PHARMACEUTICA SINICA B
(2023)
Review
Pharmacology & Pharmacy
Pavel Sokolov, Galina Nifontova, Pavel Samokhvalov, Alexander Karaulov, Alyona Sukhanova, Igor Nabiev
Summary: Multiplexed fluorescent immunohistochemical analysis of breast cancer markers and high-resolution 3D immunofluorescence imaging can provide important information for disease prognosis, therapy selection, and molecular mechanisms of carcinogenesis. The combination of fluorescent nanocrystals and single-domain antibodies as imaging nanoprobes offers significant advantages for multiplexed detection and 3D imaging of BC markers.
Article
Optics
Yinjuan Ren, Chunyang Miao, Likuan Feng, Ziming Wang, Shengli Liu, Peiran Chen, Yuting Wu, Yue Wang
Summary: A novel solution-phase technique was developed to achieve scalable halide perovskite-based MLPs, which showed great potential in laser applications. The MLPs demonstrated strong emission, low pump threshold, high Q-factor, and single-mode lasing, making them promising for optoelectronic applications.
Article
Chemistry, Multidisciplinary
I. Jennifer Gomez, Karina Ovejero-Paredes, Jose Manuel Mendez-Arriaga, Nadezda Pizurova, Marco Filice, Lenka Zajickova, Sanjiv Prashar, Santiago Gomez-Ruiz
Summary: Due to their water solubility, stability, non-toxicity, and photoluminescence, nitrogen-doped graphene quantum dots (NGQDs) have potential for delivering organotin(IV) compounds in cancer therapy. Two nanosystems were developed by grafting organotin fragments and 4-formylbenzoic acid onto the surface of NGQDs. In vitro tests demonstrated high cytotoxicity against breast cancer cells (MDA-MB-231) and minimal effects on non-cancer cells (HEK293T), with successful uptake and release of the metallodrug in cancer cells.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Multidisciplinary Sciences
Artem N. Abramov, Igor Y. Chestnov, Ekaterina S. Alimova, Tatiana Ivanova, Ivan S. Mukhin, Dmitry N. Krizhanovskii, Ivan A. Shelykh, Ivan V. Iorsh, Vasily Kravtsov
Summary: By using local deformation of monolayer WSe2, we have successfully created high-purity single-photon emitters. Through nanoindentation and atomic force microscopy, we have identified the single-photon emitting sites and reconstructed the details of the surrounding local strain potential. The experimental results suggest that the single-photon emission is likely due to strain-induced spectral shift of dark excitonic states and their hybridization with localized states of individual defects.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Kheibar Dashtian, Fatemeh Binabaji, Rouholah Zare-Dorabei
Summary: Wearable sweat sensors offer exciting opportunities for personal health monitoring and noninvasive biomarker measurements. This study presents a portable microfluidic device capable of simultaneous sweat sampling and fluorometric sensing of potential biomarkers for kidney disease monitoring. The device integrates seamlessly with smartphones for easy data retrieval and analysis. The sensors show exceptional performance, with low detection limits and remarkable selectivity. The evaluation extends to monitoring kidney diseases in real-world scenarios, showing the potential of low-cost, multidiagnostic microfluidic sensor arrays for enhanced disease detection and healthcare outcomes.
ANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
P. Schering, E. Evers, V Nedelea, D. S. Smirnov, E. A. Zhukov, D. R. Yakovlev, M. Bayer, G. S. Uhrig, A. Greilich
Summary: The study demonstrates the realization of the resonant spin amplification (RSA) effect in a specific condition, which requires intense pump pulses and can be enhanced by the spin-inertia effect. This method potentially provides the most direct and reliable tool to measure the longitudinal g factor of the charge carriers.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
