期刊
BIOMATERIALS
卷 34, 期 21, 页码 4914-4925出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.03.014
关键词
Cell tracking; Adipose-derived stem cells (ADSCs); Limb ischemia; Iron oxide nanoparticles; Magnetic resonance imaging (MRI)
资金
- National Natural Science Foundation of China [30772104]
- Natural Science Foundation of Shanghai Science and Technology Committee [11ZR1429300]
- Medical Guiding Program of Shanghai Science and Technology Committee [1141190800]
- Songjiang Medical Climbing Program, Shanghai, China [2011PD04]
- Shanghai Jiaotong University School Of Medicine Doctoral Innovation Foundation [BXJ201231]
- Ningbo Natural Science Foundation [2008A610093]
Adipose-derived stem cells (ADSCs) exhibit tremendous potential for repair of ischemic diseases. However, studies on the fate, migration, differentiation, and body distribution of the labeled ADSCs are rarely reported. In this study, magnetic iron oxide nanoparticles were designed, synthesized, and coated with meso-2,3-dimercaptosuccinic acid (DMSA) to produce DMSA nanoparticles (DMSA-NPs). The properties, size distribution, and characterization of DMSA-NPs were evaluated. Green fluorescent protein expressing ADSCs (GFP-ADSCs) were obtained and labeled with DMSA-NPs. The viability, cytotoxicity and multi-differentiation capacity of labeled GFP-ADSCs were evaluated in vitro. Labeled and non-labeled GFP-ADSCs were injected into a mouse model of hindlimb ischemia, and 3T magnetic resonance imaging (MRI) was acquired. The synthesized DMSA-NPs efficiently labeled the GFP-ADSCs in vitro and in vivo without affecting cell viability, proliferation, cell cycle, and multi-differentiation capacity. The MM showed hypointense spots in the labeled GFP-ADSCs that lasted up to 8 weeks. Prussian blue staining and immunofluorescence assay at 4 and 8 weeks indicated that the labeled GFP-ADSCs were in and around the ischemic sites and some differentiated into capillaries. This observation is identical to that seen for transplants of unlabeled cells. Labeled cells were also identified mainly in the liver and spleen, with significantly smaller amounts in the lungs, intestines, heart, and kidney. Developed DMSA-NPs were shown to exhibit a considerable potential for use as nanoprobes for MM of stem cells, which will enhance our understanding of cell-based therapeutic strategies for ischemic diseases. (C) 2013 Elsevier Ltd. All rights reserved.
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