Article
Biochemistry & Molecular Biology
Zixu Liu, Yang Liu, Huan Liu, Ruiqing Lv, Boyuan Liu, Linxuan Zhao, Tian Yin, Yu Zhang, Haibing He, Jingxin Gou, Xing Tang, Li Yang, Song Gao
Summary: This article presents a method to enhance the delivery of docetaxel (DTX) using carboxymethylcellulose (CMC)-conjugated polymeric prodrug micelles. The micelles exhibited improved drug loading, stability, and sustained release behavior, leading to reduced systemic toxicity and enhanced anti-tumor efficacy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Paul Strasser, Bianca Montsch, Silvia Weiss, Haider Sami, Christoph Kugler, Sonja Hager, Hemma Schueffl, Robert Mader, Oliver Brueggemann, Christian R. R. Kowol, Manfred Ogris, Petra Heffeter, Ian Teasdale
Summary: The design and synthesis of novel bottlebrush polymers (PPz-g-PGA) with exceptional control over their structure and molecular dimensions have been reported. These polymers show outstanding aqueous solubility, ultra-high multivalency, and biodegradability, making them ideal as nanomedicines. They exhibit enhanced cellular uptake, pharmacokinetics, and tumor accumulation, offering valuable insights into the influence of architecture on the in vivo performance of nanomedicines.
Article
Pharmacology & Pharmacy
Swati Korake, C. Bothiraja, Atmaram Pawar
Summary: PEG-decorated solid lipid nanoparticles were developed to enhance the biopharmaceutical properties of docetaxel, showing improved bioavailability and anticancer activity.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Review
Engineering, Biomedical
Bianka Golba, Edmondo M. Benetti, Bruno G. De Geest
Summary: Cyclic polymers, lacking chain ends, possess unique architecture and properties that make them suitable for various applications in nano-, micro-, and macro-scale materials. In the biomedical field, biomaterials based on cyclic polymers demonstrate distinct properties in terms of drug delivery efficiency and surface activity. Future challenges in utilizing cyclic polymers in biomedical applications are also discussed in this review.
Article
Biotechnology & Applied Microbiology
Rita M. Pinto, Saleh Yazdani, Catarina Leal Seabra, Martine De Jonge, Mukaddes Izci, Rebeca Cruz, Susana Casal, Stefaan J. Soenen, Salette Reis, Claudia Nunes, Patrick Van Dijck
Summary: Staphylococcus aureus is a high priority pathogen due to its prevalence and ability to form biofilms. Current treatments do not target the matrix that contributes to antimicrobial tolerance. This study proposes the use of lipid nanoparticles encapsulating caspofungin to disrupt the matrix, and combining them with moxifloxacin-loaded nanoparticles to reduce biofilms. In vitro and in vivo studies showed promising results, with reduced biofilm formation and no bacterial dissemination into vital organs.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Pharmacology & Pharmacy
Ritu R. Kudarha, Krutika K. Sawant
Summary: The study demonstrated that the HA-TNPs have potential for targeted delivery to brain tumors with improved cellular uptake and enhanced antitumor activity compared to pure TMZ.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Paula S. Ferreira, John A. Manthey, Marina S. Nery, Thais B. Cesar
Summary: The study demonstrated the metabolism and distribution of orally administered eriocitrin in rats for the first time, identifying multiple metabolites in rat urine and tissues, with homoeriodictyol and homoeriodictyol-7-O-glucuronide as the major metabolites. The half-lives of metabolites in plasma were approximately 3 to 3.2 hours, and the total bioavailability of eriocitrin was less than 1%.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Qiangqiang Shi, Chengzhou Song, Minglong Chen, Jie Xu, Shaoqiu Zheng, Jiajia Tan, Jialin Zhang, Ning Wang, Jinming Hu, Shiyong Liu
Summary: Polymeric nanoparticles (NPs) are commonly used for drug delivery, and their biological behavior is usually tracked using fluorescence or isotope labeling methods. However, these labeling techniques may affect the properties of NPs and result in inaccurate information. It is therefore crucial to develop label-free techniques for accurately assessing the biological fate of polymeric NPs. In this study, discrete oligourethane amphiphiles (DOAs) were used to create nanorods (NRs) with distinct surface functional groups. These NRs can be quantified without labeling and their surface functional groups were found to significantly impact their biological behavior.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Vincent Mittelheisser, Pierre Coliat, Eric Moeglin, Lilian Goepp, Jacky G. Goetz, Loic J. Charbonniere, Xavier Pivot, Alexandre Detappe
Summary: Tumor-targeted antibody/fragment-conjugated nanoparticles show promise in improving local delivery of small molecules. A meta-analysis of 161 studies confirmed that targeted nanoparticles had significantly higher tumor uptake compared to naked nanoparticles. The study also found that the choice of nanoparticle type, size, and antibody moiety affected tumor uptake.
ADVANCED MATERIALS
(2022)
Article
Engineering, Biomedical
Fei Wang, Ling Sum Liu, Pan Li, Cia Hin Lau, Hoi Man Leung, Y. Rebecca Chin, Chung Tin, Pik Kwan Lo
Summary: Compared with siRNAs or other ASOs, TNA has the advantages of chemical simplicity, DNA/RNA binding capability, folding ability of tertiary structure, and excellent physiological stability, which make it a promising tool in biomedical applications. This study investigates the pharmacokinetics and biosafety of TNAs in living organisms, finding that they exhibit excellent stability, low cytotoxicity, and substantial uptake in living cells without transfection. TNAs also show the ability to efficiently penetrate multicellular spheroids and can be safely administered in mice without causing pathological changes in the kidneys.
MATERIALS TODAY BIO
(2022)
Article
Pharmacology & Pharmacy
Imran Rather, Nusrat Shafiq, Jaya Shukla, Gurvinder Kaur, Somit Pandey, Ritika Kondel Bhandari, Avaneesh Kumar Pandey, B. R. Mittal, G. K. Khuller, Navneet Sharma, Samir Malhotra
Summary: This study explores the pharmacokinetics and biodistribution of poly(lactic-co-glycolic) acid (PLGA) nanoparticles in healthy human volunteers. The results show significant differences in pharmacokinetic parameters between the nanoparticle and conventional groups, with the nanoparticles mainly distributed in the distal portions of the intestine, making them potentially advantageous for the treatment of intestinal or lymph node tuberculosis.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu-Chuen Huang, Pei-Ying Hsieh, Li-Ying Wang, Tung-Hu Tsai, Yu-Jen Chen, Chen-Hsi Hsieh
Summary: The aim of this study was to evaluate the radiotherapy (RT)-pharmacokinetics (PK) effect of cabozantinib in concurrent or sequential regimens with external beam radiotherapy (EBRT) or stereotactic body radiation therapy (SBRT). The RT-drug interactions of cabozantinib under RT were confirmed in a free-moving rat model. The biodistribution of cabozantinib in the heart is modulated by off-target irradiation and SBRT doses simultaneously.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Polymer Science
Zheng Li, Ying-Wei Yang
Summary: Conjugated macrocycle polymers (CMPs) are a type of organic porous materials with supramolecular macrocycles embedded in the conjugated skeleton, offering flexibility for molecular design and customized functionalization. They have shown promise in various applications and have sparked growing interest in the research community.
Article
Pharmacology & Pharmacy
Anna Maria Tolomeo, Gaia Zuccolotto, Ricardo Malvicini, Giada De Lazzari, Alessandro Penna, Chiara Franco, Federico Caicci, Fabio Magarotto, Santina Quarta, Michela Pozzobon, Antonio Rosato, Maurizio Muraca, Federica Collino
Summary: This study aimed to evaluate the biodistribution of MSC-EVs after different routes of administration in mice. Results showed that local administration could increase the concentration of EVs in selective organs, limiting their systemic distribution and potentially reducing extra-organ effects. Biodistribution studies can help in selecting the most appropriate way of administering MSC-EVs for treating different diseases.
Article
Chemistry, Multidisciplinary
Jiayi Zhu, Zhipeng Zhao, Huarui Chen, Xinglin Chen, Jinbin Liu
Summary: This study demonstrates that renal-clearable nanomedicines show a more sensitive response to injection dose and have improved tumor-targeting efficiency compared to nonrenal-clearable nanoparticles.
Review
Engineering, Biomedical
Anne Nguyen, Roland Bottger, Shyh-Dar Li
Summary: Prodrugs are designed to improve the pharmaceutical properties of potent compounds and represent a central approach in drug development. The success of prodrug strategy relies on reversible linkages for controlled release of the parent drug. While prodrug approaches enhance pharmacokinetic properties over their parent drugs, challenges still exist in absorption, distribution, metabolism, elimination, and toxicity.
Article
Medicine, Research & Experimental
Nojoud Al Fayez, Roland Bottger, Elham Rouhollahi, Pieter R. Cullis, Dominik Witzigmann, Shyh-Dar Li
Summary: The study demonstrated that phospholipid-free small unilamellar vesicles composed of Tween80 and cholesterol could effectively encapsulate and deliver primaquine (PQ) to hepatocytes, reducing exposure to red blood cells and hemolytic toxicity. This approach showed potential in improving the efficacy of PQ for treating liver-stage malaria.
MOLECULAR PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Yael Nicole Slavin, Kristina Ivanova, Wei-lun Tang, Tzanko Tzanov, Shyh-dar Li, Horacio Bach
Summary: The study developed a new platform based on niosomes loaded with antimicrobial agents to target intracellular mycobacteria. Nanoniosomes showed efficacy in treating Mycobacterium abscessus in infected macrophages without upregulation of pro-inflammatory cytokines, suggesting their potential for further testing in precise intracellular drug delivery.
Article
Pharmacology & Pharmacy
Griffin Pauli, Po-Han Chao, Zhu Qin, Roland Bottger, Suen Ern Lee, Shyh-Dar Li
Summary: A liposome-based delivery system containing a toll-like receptor agonist, resiquimod, has been developed to enhance immune response against colorectal cancer with peritoneal metastases. This system localized the agonist in the peritoneal cavity, leading to decreased systemic absorption and increased peritoneal retention, resulting in an enhanced antitumor effect.
Article
Engineering, Biomedical
Anne Nguyen, Po-Han Chao, Chun Yat Ong, Elham Rouhollahi, Nojoud AL Fayez, Louis Lin, Jennifer I. Brown, Roland Bottger, Brent Page, Harvey Wong, Shyh-Dar Li
Summary: This study utilized click and steric hindrance chemistry to modulate drug release rate, conjugating mPEG with PTX to form PTX-NPs with different chemical modalities. Among them, PTX-R1-NP showed the best pharmacokinetic profiles and antitumor effects.
Article
Pharmacology & Pharmacy
Nojoud Al Fayez, Roland Bottger, Sreemoyee Ghosh, Yushi Nakajima, Po-Han Chao, Elham Rouhollahi, Anne Nguyen, Pieter R. Cullis, Dominik Witzigmann, Shyh-Dar Li
Summary: This study reports a new method based on a liposomal multilamellar vesicle platform to prepare child-friendly oral formulations. The technique successfully converted weak-base drugs into liquid preparations and prevented drug taste interference by encapsulating the drugs completely. The formulation could be stored at room temperature and released the drug effectively in vitro and in vivo.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Editorial Material
Medicine, Research & Experimental
Shyh-Dar Li, Ellen Wasan, Marcel Bally, Sarah Hedtrich
MOLECULAR PHARMACEUTICS
(2022)
Review
Instruments & Instrumentation
K. Laxmi Swetha, Kavya Sree Maravajjala, Shyh-Dar Li, Manu Smriti Singh, Aniruddha Roy
Summary: Most current antitumor therapies target cancer cells only, but they are often found to be ineffective in the majority of tumors. Advanced research has shown that cancer is a multicellular disorder and the tumor microenvironment (TME) plays a crucial role in tumor progression. Recent studies suggest that multidimensional therapy targeting multiple components of the TME with drug combinations can be highly effective, and nanomedicine could be utilized for targeted delivery of such therapies.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Nojoud A. L. Fayez, Roland Bottger, Jennifer Brown, Elham Rouhollahi, Shyh-Dar Li
Summary: This study elucidated the hepatocyte targeting mechanism of phospholipid-free small unilamellar vesicles (PFSUVs) and identified the key role of apolipoprotein AII (ApoA2). The study demonstrated that PFSUVs adsorbed plasma ApoA2 to their surface, which was recognized by the scavenger receptor B-1 (SR-B1) expressed on hepatocytes and internalized through clathrin-mediated endocytosis.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Nojoud Al Fayez, Elham Rouhollahi, Chun Yat Ong, Jiamin Wu, Anne Nguyen, Roland Bottger, Pieter R. Cullis, Dominik Witzigmann, Shyh-Dar Li
Summary: This study demonstrated the use of lipid-based nanoparticles (LNPs) to deliver Imiquimod (IMQ) and increase IFN-alpha production in the liver. The hepatocyte-targeted LNPs showed high selectivity and significantly reduced serum levels of HBsAg, indicating their potential for treatment of chronic hepatitis B.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Zhaomeng Wang, Yanhua Liu, Shuo Li, Fanlin Meng, Jiang Yu, Shuang Zhou, Songtao Dong, Jianying Ye, Lingxiao Li, Chuang Zhang, Shengnan Qiu, Guimei Lin, Hongzhuo Liu, Shyh-Dar Li, Zhonggui He, Yongjun Wang
Summary: Inspired by natural phenolic-protein interactions, the study developed an albumin-bound technology based on phenolic acid derivatives. These derivatives can bind to albumin and form nanocomplexes. The technology has been successfully applied to various anticancer drugs and showed promising results in breast cancer models.
Article
Engineering, Biomedical
Gubran Khalil Mohammed, Roland Bottger, Andor Krizsan, Daniela Volke, Marina Moetzing, Shyh-Dar Li, Daniel Knappe, Ralf Hoffmann
Summary: The prodrug strategy of linking polyethylene glycol (PEGs) with proline-rich AMP Onc72 is tested for the first time in vivo. The PEGylation protects Onc72 from degradation and provides a prolonged release, leading to stable Onc72 concentrations and high antibacterial activities. The prodrugs show promising safety margins and extended elimination half-life in mice.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jiamin Wu, Natalie Jones, Nojoud A. L. Fayez, Po-Han Chao, Angeline Wu, Daniele Ribeiro de Araujo, Elham Rouhollahi, Analisa Jia, Shyh-Dar Li
Summary: We propose a non-parenteral delivery method for proteins using protamine, an FDA-approved peptide. Protamine promotes actin tubulation and rearrangement, enhancing intracellular protein delivery compared to poly(arginine)(8) (R8). Protamine directs proteins to the nuclei with minimal lysosomal uptake, in contrast to R8-mediated delivery. Intranasal delivery of insulin mixed with protamine effectively reduces blood glucose levels in diabetic mice for up to 6 hours, similar to subcutaneously injected insulin.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Materials Science, Biomaterials
Anne Nguyen, Elham Rouhollahi, Roland Bottger, Chun Yat Ong, Po-Han Chao, Jiamin Wu, Yao Chen, Shyh-Dar Li
Summary: Conjugating poorly water-soluble small hydrophobic compounds with hydrophilic polymers can improve their aqueous solubility and circulation time. This study investigates the influence of polymer molecular weight and linker on the toxicity and drug release of conjugates. Using a click chemistry platform, mPEG of different molecular weights were conjugated to an anti-tumor drug, resulting in nanoparticles with optimal anti-tumor efficacy for mPEG(5000) and an ester linker.
BIOMATERIALS SCIENCE
(2022)
Article
Materials Science, Biomaterials
Anne Nguyen, Hidenori Ando, Roland Bottger, K. K. DurgaRao Viswanadham, Elham Rouhollahi, Tatsuhiro Ishida, Shyh-Dar Li
BIOMATERIALS SCIENCE
(2020)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.