期刊
BIOMATERIALS
卷 33, 期 33, 页码 8486-8494出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.07.051
关键词
Multimodality imaging; Magnetic nanoparticle; Quantum dot; Breast cancer tumor; NIR; MRI
资金
- Ministry and Education, Science, Sport, and Culture of Japan [19550157]
- National Natural Science Foundation of China [21005029]
- Japan Society for the Promotion of Science [L11525]
- Grants-in-Aid for Scientific Research [23370072, 19550157] Funding Source: KAKEN
Multilayered, core/shell nanoprobes (MQQ-probe) based on magnetic nanoparticles (MNPs) and quantum dots (QDs) have been successfully developed for multimodality tumor imaging. This MQQ-probe contains Fe3O4 MNPs, visible-fluorescent QDs (600 nm emission) and near infrared-fluorescent QDs (780 nm emission) in multiple silica layers. The fabrication of the MQQ-probe involves the synthesis of a primer Fe3O4 MNPS/SiO2 core by a reverse microemulsion method. The MQQ-probe can be used both as a fluorescent probe and a contrast reagent of magnetic resonance imaging. For breast cancer tumor imaging, anti-HER2 (human epidermal growth factor receptor 2) antibody was conjugated to the surface of the MQQ-probe. The specific binding of the antibody conjugated MQQ-probe to the surface of human breast cancer cells (KPL-4) was confirmed by fluorescence microscopy and fluorescence-activated cell sorting analysis in vitro. Due to the high tissue permeability of near-infrared (NIR) light, NIR fluorescence imaging of the tumor mice (KPL-4 cells transplanted) was conducted by using the anti-HER2 antibody conjugated MQQ-probe. In vivo multimodality images of breast tumors were successfully taken by NIR fluorescence and T-2-weighted magnetic resonance. Antibody conjugated MQQ-probes have great potential to use for multimodality imaging of cancer tumors in vitro and in vivo. (C) 2012 Elsevier Ltd. All rights reserved.
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