Article
Agriculture, Multidisciplinary
Qian Liu, Heping Cui, Bertrand Muhoza, Emmanuel Duhoranimana, Khizar Hayat, Xiaoming Zhang, Chi-Tang Ho
Summary: Low-environment-sensitive nanoparticles were prepared by enzymatic cross-linking of dextran-grafted whey protein isolate and chondroitin sulfate. Transglutaminase (TG) and laccase cross-linking effects on nanoparticle stability were investigated. TG cross-linking and grafted dextran contributed to stability against environmental conditions, while laccase treatment did not enhance stability.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Analytical
Lu Yao, Min Liao, Jing-Kang Wang, Jing Wang, Dan Liu, Peng-Fei Tu, Ke-Wu Zeng
Summary: Cellular target identification is crucial for drug development and understanding pharmacological mechanisms. In this study, a gold nanoparticle-based photo-cross-linking strategy was employed to identify neuroprotective targets, resulting in the discovery of 13 potential proteins, including Hsp60, which was further screened for binding compounds from phytochemicals.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Tobias A. A. Bauer, Irina Alberg, Lydia A. A. Zengerling, Pol Besenius, Kaloian Koynov, Bram Slutter, Rudolf Zentel, Ivo Que, Heyang Zhang, Matthias Barz
Summary: Core cross-linked polymeric micelles (CCPMs) are designed to improve the therapeutic profile of hydrophobic drugs, reduce or completely avoid protein corona formation, and offer prolonged circulation times. CCPM stability was tuned by using bifunctional or trifunctional cross-linkers and varying the cross-linkable polymer block length. The circulation time and biodistribution of CCPMs can be enhanced by using trivalent peptide cross-linkers.
Review
Chemistry, Multidisciplinary
Wei-Hua Han, Qing-Yu Wang, Yuan-Yi Kang, Li-Rui Shi, Yu Long, Xin Zhou, Chun-Cheng Hao
Summary: This review comprehensively discusses the cross-linking electrospinning system for the first time, including cross-linking strategies, cross-linking mechanisms, and applications of cross-linked electrospun nanofibers. The significance of cross-linking electrospinning and the challenges in constructing this system are also highlighted.
Article
Chemistry, Multidisciplinary
Mengyao Zhao, Hongjun Zhuang, Benhao Li, Meiwan Chen, Xiaoyuan Chen
Summary: A supramolecular coordination-induced nanoparticle cross-linking strategy is developed for enhanced photodynamic therapy by utilizing a complementary functioning tumor microenvironment. The coordinated interaction between manganese oxide and polyhydroxy photosensitizer hypericin improves the energy transfer efficiency and enhances the intracellular accumulation of both upconversion nanoparticles and hypericin. This strategy realizes the function amplification of each component after responsive transformation and offers a new avenue for enhanced cancer therapy.
ADVANCED MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
A. Ramos-Valle, L. Marin-Caba, L. Garcia Hevia, M. A. Correa-Duarte, M. L. Fanarraga
Summary: Repairing genetic defects using exogenous DNA is a major challenge, and the design of effective vectors for encapsulating and delivering DNA to specific cells is crucial. The silica-based particles developed in this study provide stable encapsulation and protection of DNA, even under various stress conditions. The versatility of the design allows for the sequential expression of multiple genes, mimicking viral gene expression cascades, and the method is scalable, reproducible, and cost-effective, making it valuable for the personalized therapeutics industry.
MATERIALS TODAY ADVANCES
(2023)
Article
Pharmacology & Pharmacy
Michael Karimov, Dietmar Appelhans, Alexander Ewe, Achim Aigner
Summary: This study explores the combination of cross-linking small PEI molecules with biodegradable disulfide-groups and modifying them with tyrosine to enhance DNA delivery efficiency. The synergistic effects of these modifications result in improved transfection efficacy and biocompatibility, making them promising for therapeutic applications.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Chemistry, Applied
Dongdong Feng, Zhecheng Zhang, Xishan Zhu, Yu Zhang, Zhen Luo, Lixiao Luo, Wenda Zhang, Chunfei Wu, Jianmin Gao
Summary: CCUS technology is a crucial method for achieving carbon neutral energy utilization. The use of ammonia CO2-capture method, though mature, has limitations such as low CO2 absorption efficiency and ammonia escape. A novel ethanol/biochar synergistic ammonia carbon capture technology is proposed, and through wetted-wall column experiments, it is proven that biochar and ethanol have a synergistic effect on CO2 absorption by ammonia. The simulation indicates that the micropores in biochar adsorb and enrich CO2, while mesopores and macropores facilitate CO2 diffusion and transport, enhancing the mass transfer capacity of CO2. The experimental results show that the mass transfer efficiency of biochar-ethanol is increased by 15.4%, surpassing that of ethanol (7.1%), but ammonia escape is more pronounced. The enhancement effect of biochar's pore structure on CO2 diffusion into ammonia could be a promising direction for improving CO2 capture by ammonia.
FUEL PROCESSING TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Johannes F. Hevler, Marie Lukassen, Alfredo Cabrera-Orefice, Susanne Arnold, Matti F. Pronker, Vojtech Franc, Albert J. R. Heck
Summary: Cross-linking mass spectrometry has become an important method for studying protein structures and interactions, with the combination of blue native PAGE and in-gel cross-linking mass spectrometry (IGX-MS) offering a more efficient workflow that avoids certain time-consuming steps. IGX-MS not only reduces the unwanted over-length cross-links compared to traditional in-solution cross-linking, but also allows for the parallel analysis of co-occurring protein complexes using minimal sample amounts. Additionally, IGX-MS has been shown to provide new insights into the initial stages of the terminal complement pathway through experiments on complement components C5, C6, and C5b6 complex.
Article
Multidisciplinary Sciences
Haijun Liu, Mengru M. Zhang, Daniel A. Weisz, Ming Cheng, Himadri B. Pakrasi, Robert E. Blankenship
Summary: In cyanobacteria and red algae, the structural basis of efficient excitation energy transfer from the phycobilisome (PBS) antenna complex to the reaction centers is still unclear. A tricylindrical cyanobacterial PBS core structure was proposed using chemical cross-linking mass spectrometry and homology modeling, revealing the essential roles of anchoring domains in facilitating energy transfer to photosystem II (PSII) and PSI. The uneven bottom surface of the PBS core allows for increased accessibility for regulatory elements, such as the orange carotenoid protein, necessary for modulating photochemical activity.
Article
Chemistry, Multidisciplinary
Ian Hamilton, Magdalena Gebala, Daniel Herschlag, Rick Russell
Summary: We developed a disulfide cross-linking approach, xHEED, to measure the distance-dependent encounter frequency of DNA helices in solution. Our measurements define the relevant distances for electrostatic interactions of nucleic-acid helices and provide insights into the impact of different ionic conditions on these forces.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Jonas Kost, Alexander Bleiziffer, Dennis Rusitov, Juergen Ruehe
Summary: This study focuses on the thermal activation of surface-attached polymer networks under mild conditions, using a novel cross-linker based on a diazo phenyl ester group. The system, which enables the rapid complete cross-linking of films with simultaneous surface attachment to organic materials via CHic, only requires activation below 100 degrees C and has a long shelf life at room temperature. It has potential applications in coatings and complex geometries.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Yulong Huang, Yuxuan Chen, Yong Hu, Travis Mitchell, Lu An, Zheng Li, Jason Benedict, Huashan Li, Shenqiang Ren
Summary: A promising design strategy for next-generation molecular magnetoelectrics has been proposed, which integrates molecular magnetism and electric conductivity through an in situ cross-linking strategy. This approach has successfully synthesized flexible molecular-based magnetoelectronics by cross-linking magnetic coordination networks that incorporate conducting chain building blocks, achieving significant improvements.
Article
Biochemical Research Methods
Hongyi Zhu, Vaclav Zima, Emily R. Ding, Frantisek Turecek
Summary: Scaffolds were synthesized and used for the study of intramolecular peptide-carbene cross-linking in gas-phase cations. Carbene intermediates were generated by UV-laser photo-dissociation and the cross-linked products were detected and quantified. The presence of certain residues affected the yield of cross-linked products. Experiments and analysis revealed that cross-links involved specific amino acid residues and functional groups.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Multidisciplinary Sciences
Jasjot Singh, Hadeer Elhabashy, Pathma Muthukottiappan, Markus Stepath, Martin Eisenacher, Oliver Kohlbacher, Volkmar Gieselmann, Dominic Winter
Summary: Lysosomes are crucial organelles for degradation and metabolism regulation. Protein complexes play a decisive role in lysosomal function. This study investigates lysosomes and early endosomes using cross-linking mass spectrometry, revealing protein interactions, structures, and cargo of flotillin-mediated endocytosis.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Yan Huang, Chengtie Wu, Xiaoling Zhang, Jiang Chang, Kerong Dai
ACTA BIOMATERIALIA
(2018)
Article
Biochemistry & Molecular Biology
De Li, Hui Wang, Zhuokai Li, Chenglong Wang, Fei Xiao, Yuan Gao, Xiang Zhang, Peng Wang, Jianping Peng, Guiquan Cai, Bin Zuo, Yun Shen, Jin Qi, Niandong Qian, Lianfu Deng, Weidong Song, Xiaoling Zhang, Lei Shen, Xiaodong Chen
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Endocrinology & Metabolism
Shihui Chen, Lizhen Zheng, Jiayong Zhang, Heng Wu, Nan Wang, Wenxue Tong, Jiankun Xu, Le Huang, Yifeng Zhang, Zhijun Yang, Ge Lin, Xinluan Wang, Ling Qin
Article
Cell Biology
Chuandong Wang, Fei Xiao, Yaokai Gan, Weien Yuan, Zhanjing Zhai, Tuo Jin, Xiaodong Chen, Xiaoling Zhang
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Cell Biology
Fei Xiao, Chuandong Wang, Chenglong Wang, Yuan Gao, Xiaoling Zhang, Xiaodong Chen
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Yuan Gao, Fei Xiao, Chenglong Wang, Chuandong Wang, Penglei Cui, Xiaoling Zhang, Xiaodong Chen
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Article
Plant Sciences
Yulei Cui, Xiaoying Zhao, Lijuan Mei, Jinjin Pei, Shuo Wang, Yun Shao, Yanduo Tao, Xiaoling Zhang, Lei Jiang
JOURNAL OF ETHNOPHARMACOLOGY
(2018)
Article
Oncology
Yongming Xu, Shujuan Xu, Zhi Gao, Liang Xiao, Fei Xiao, Hongguang Xu, Xiaoling Zhang
MOLECULAR MEDICINE REPORTS
(2018)
Article
Multidisciplinary Sciences
Yujie Deng, Jinqiu Lu, Wenling Li, Ailing Wu, Xu Zhang, Wenxue Tong, Kiwai Kevin Ho, Ling Qin, Hai Song, Kinglun Kingston Mak
NATURE COMMUNICATIONS
(2018)
Article
Rheumatology
Wenxue Tong, Yelin Zeng, Dick Ho Kiu Chow, Wai Yeung, Jiankun Xu, Yujie Deng, Shihui Chen, Hui Zhao, Xiaoling Zhang, Kevin Kiwai Ho, Ling Qin, Kingston King-Lun Mak
ANNALS OF THE RHEUMATIC DISEASES
(2019)
Article
Pharmacology & Pharmacy
Jin Long Liu, Jing Li, Jia Jia Xu, Fei Xiao, Peng Lei Cui, Zhi Guang Qiao, Xiao Dong Chen, Wei Dong Tao, Xiao Ling Zhang
MOLECULAR PHARMACOLOGY
(2019)
Article
Cell Biology
Penglei Cui, Xiaoying Zhao, Jinlong Liu, Xiaodong Chen, Yuan Gao, Kun Tao, Chuandong Wang, Xiaoling Zhang
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Danyang Zhao, Wenbo Jiang, Yu Wang, Chuandong Wang, Xiaoling Zhang, Qingfeng Li, Dong Han
BIOMED RESEARCH INTERNATIONAL
(2020)
Article
Cell Biology
Yuhang Liu, Xudong Zhang, Xiaodong Chen, Bingjun Zhang, Liming Dai, Chenglong Wang, Yang Li, Xiaoling Zhang
Summary: MicroRNAs are crucial in the chondrogenesis of bone marrow mesenchymal stem cells (MSCs) and regulate joint regeneration in osteoarthritis. This study aimed to determine the role of miR146a in the chondrogenic differentiation of MSCs and its underlying mechanisms. The results showed that miR146a inhibits chondrogenesis in MSCs by directly targeting Lsm11.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
