Article
Engineering, Biomedical
N. Contessi Negrini, A. Angelova Volponi, C. A. Higgins, P. T. Sharpe, A. D. Celiz
Summary: Developmental TE is a novel paradigm aiming to obtain tissues and organs with correct biomorphology and biofunctionality by mimicking the morphogenetic processes leading to the tissue/organ generation in the embryo. Successful applications have been reported for ectodermal appendages such as teeth, hair follicles and glands, with a focus on accurate selection of cell sources, scaffolds, and cell culture configurations to replicate in vivo morphogenetic cues.
MATERIALS TODAY BIO
(2021)
Article
Biotechnology & Applied Microbiology
Haojie Fu, Lin Sen, Fangqi Zhang, Sirui Liu, Meiyue Wang, Hongyan Mi, Mengzhe Liu, Bingyan Li, Shumin Peng, Zelong Hu, Jingjing Sun, Rui Li
Summary: This study constructed xenogeneic bioengineered tooth roots and used extracellular vesicles from human adipose-derived mesenchymal stem cells (hASC-EVs) to protect the bio-roots from oxidative damage. The results showed that hASC-EVs reduced cell apoptosis and oxidative stress, improved cell proliferation and antioxidant capacity, and suppressed oxidative damage through the activation of the PI3K/Akt and NRF2 pathways. In vivo experiments demonstrated that hASC-EV administration enhanced the antioxidant effect of the bio-root and promoted the regeneration of periodontal ligament-like tissue.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Christian Morsczeck
Summary: This article summarizes the molecular mechanisms driving osteogenic differentiation of dental follicle cells (DFCs) and discusses other factors that influence this process, providing valuable insights for future research and applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
X. Lin, Q. Li, L. Hu, C. Jiang, S. Wang, X. Wu
Summary: The study reveals the crosstalk between dental pulp and dental follicle through the OGN-Hh pathway, which regulates the stemness and multidirectional differentiation of dental follicle stem cells. Ogn(-/-) mice exhibit accelerated root growth rate and thicker hard tissues in the root, suggesting the activation of the Hh pathway and increased cell proliferation and differentiation. This study provides important insights into the molecular regulation of early periodontal tissue development and lays a theoretical foundation for tooth root biology and periodontal tissue regeneration research.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Cell Biology
Kajohnkiart Janebodin, Rakchanok Chavanachat, Aislinn Hays, Morayma Reyes Gil
Summary: The study aimed to investigate the role of VEGFR-2 in odontoblast differentiation of dental pulp stem cells (DPSCs) by knocking down the expression of VEGFR-2. The results showed that VEGFR-2 is crucial for odontoblast differentiation in DPSCs, and deficiency of VEGFR-2 may impair the differentiation of odontoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiuqun Wei, Shujuan Guo, Qian Liu, Li Liu, Fangjun Huo, Yafei Wu, Weidong Tian
Summary: Dental follicle stem cells (DFSCs) promote periodontal regeneration in an inflammatory microenvironment by expressing periostin and improving the immune microenvironment through macrophage recruitment and reprogramming. Silencing periostin attenuates the effects of DFSCs in promoting periodontal regeneration and regulating macrophages. Recombinant human periostin (rhPeriostin) can directly promote macrophage reprogramming and show potential for promoting periodontal regeneration when loaded in a collagen matrix.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
S. M. Ziauddin, Misako Nakashima, Hideto Watanabe, Michiyo Tominaga, Koichiro Iohara
Summary: This study compared the stem cell properties and pulp regenerative potential of DT-DPSCs and PT-DPSCs from the same individual. The results showed that DT-DPSCs exhibited higher expression of stem cell markers and angiogenic factors compared to PT-DPSCs. DT-DPSC-derived conditioned medium also promoted increased cell proliferation, migration, and angiogenesis. Furthermore, the regenerated pulp tissues in both DT-DPSCs and PT-DPSCs groups were similar. Therefore, DT-DPSCs could be a potential alternative to PT-DPSCs for pulp regenerative therapy.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Chemistry, Multidisciplinary
Ik-Hwan Kim, Mijeong Jeon, Kyounga Cheon, Sun Ha Kim, Han-Sung Jung, Yooseok Shin, Chung Min Kang, Seong-Oh Kim, Hyung-Jun Choi, Hyo-Seol Lee, Ko Eun Lee, Je Seon Song
Summary: This study evaluated the use of decellularized human tooth as a scaffold for regeneration of periodontal ligaments (PDL) and pulp tissue with PDLSCs and DPSCs, respectively. The experiment demonstrated successful differentiation and potential for regeneration in both PDLSCs and DPSCs on the decellularized tooth scaffolds. This suggests a novel treatment method for tooth regeneration utilizing decellularized human tooth as a scaffold.
APPLIED SCIENCES-BASEL
(2021)
Article
Cell & Tissue Engineering
Genzheng Yi, Siyuan Zhang, Yue Ma, Xueting Yang, Fangjun Huo, Yan Chen, Bo Yang, Weidong Tian
Summary: This study found that matrix vesicles (MVs) can effectively improve the proliferation, migration, and osteogenic differentiation of dental follicle cells (DFCs), with collagenase-released MVs (CRMVs) showing better bone regeneration capabilities. In a rat alveolar bone defect model, CRMVs loaded on a collagen scaffold had more significant therapeutic outcomes compared to media MVs (MMVs) and DFCs-based treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Engineering, Biomedical
Qian Li, Zhiai Hu, Yongxi Liang, Cancan Xu, Yi Hong, Xiaohua Liu
Summary: The study developed a multifunctional peptide-conjugated, pH-sensitive non-viral gene vector for fast revascularization and pulp regeneration in a full-length human tooth root. This vector significantly increased gene transfection efficiency, reduced cytotoxicity, upregulated VEGF expression in dental pulp stem cells, and accelerated new blood vessel formation and pulp tissue regeneration. The approach proves to be safe and effective for regenerative endodontics.
ACTA BIOMATERIALIA
(2021)
Review
Cell Biology
Cangwei Liu, Hao Guo, Ce Shi, Hongchen Sun
Summary: Short root anomaly (SRA) can cause tooth loss and affect patients' health. Dental implants have limitations compared to natural tooth roots, and tissue engineering-mediated root regeneration offers a more natural function. This article reviews the roles of Bone Morphogenetic Proteins (BMPs) in tooth root development and regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ana Catarina Costa, Patricia Mafalda Alves, Fernando Jorge Monteiro, Christiane Salgado
Summary: Oral-maxillofacial tumor removal can result in bone defects and functional problems. This study developed a bioengineered construct loaded with dental stem cells and a biomimetic hydrogel for tissue imaging in live animals. The bioconstruct promoted oral osteomucosa tissue regeneration and provided insights for patient-specific therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Irina Lupse, Emoke Pall, Lucian Barbu Tudoran, Adriana Elena Bulboaca, Andreea Ciurea, Iulia Cristina Micu, Alexandra Roman, Ada Gabriela Delean, Alexandrina Muntean, Andrada Soanca
Summary: The three antimicrobial products tested showed dose-dependent cytotoxicity on gMSCs and affected cell proliferation and viability. Significant differences were observed in CCK8 values, rejecting the null hypothesis. However, there were no significant differences in MTT values between the three products.
Article
Cell Biology
Rung-Shu Chen, Sheng-Hao Hsu, Hao-Hueng Chang, Min-Huey Chen
Summary: The study aimed to explore the feasibility of using adult dental pulp stem cells on polylactic acid scaffolds for tooth regeneration through experiments on dogs. The mineralization degree in the experimental group with cells seeded in the scaffolds was significantly higher than the control group, indicating the importance of dental pulp stem cells for tooth regeneration. However, there was incomplete absorption of the HA/PLA scaffolds in both groups, suggesting the need for a more rapidly absorbable material in scaffold design.
Review
Dentistry, Oral Surgery & Medicine
Yuejiao Xin, Na Zhao, Yixiang Wang
Summary: RUNX2 plays a pivotal and complex role in regulating tooth eruption. Aberrant expression of RUNX2 can lead to disrupted or failed tooth eruption. It promotes osteogenesis in the radicular portion of the dental follicle and regulates osteoclastogenesis and the formation of the eruption pathway.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.