期刊
BIOMATERIALS
卷 33, 期 20, 页码 5136-5143出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.03.037
关键词
Biofabrication; Biofilm mimics; Cell signaling; Quorum sensing; Chitosan membrane; Alginate hydrogel
资金
- Robert W. Deutsch Foundation
- Defense Threat Reduction Agency (DTRA) [BO08SPO008]
- Office of Naval Research (ONR) [N000141010446]
- NSF-EFRI [EFRI-0735987]
- Maryland NanoCenter
- Maryland NanoCenter FabLab
- Directorate For Engineering
- Emerging Frontiers & Multidisciplinary Activities [1042881] Funding Source: National Science Foundation
Signaling between cells guides biological phenotype. Communications between individual cells, clusters of cells and populations exist in complex networks that, in sum, guide behavior. There are few experimental approaches that enable high content interrogation of individual and multicellular behaviors at length and time scales commensurate with the signal molecules and cells themselves. Here we present biofabrication in microfluidics as one approach that enables in-situ organization of living cells in microenvironments with spatiotemporal control and programmability. We construct bacterial biofilm mimics that offer detailed understanding and subsequent control of population-based quorum sensing (QS) behaviors in a manner decoupled from cell number. Our approach reveals signaling patterns among bacterial cells within a single biofilm as well as behaviors that are coordinated between two communicating biofilms. We envision versatile use of this biofabrication strategy for cell-cell interaction studies and small molecule drug discovery. (C) 2012 Elsevier Ltd. All rights reserved.
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