期刊
BIOMATERIALS
卷 33, 期 22, 页码 5514-5523出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.04.001
关键词
T3; PI3K/AKT signal pathway; Induced pluripotent stem cell; iPSC; Reprogramming; Metabolic remodeling
资金
- Shenzhen International Collaboration Grant [GJ200807210024A]
- California Institute of Regenerative Medicine (CIRM) [RT2-01942]
- NIH [1R43 CA103553-01]
- Department of Defense [W81XWH-04-1-0597]
- Research Service of the Department of Veterans Affairs
Generation of induced pluripotent stem cells (iPSCs) from somatic cells by defined factors is a mechanism-unknown, yet extremely time-consuming process. Inefficient reprogramming leads to prolonged periods of in vitro iPSC selection, resulting in subtle genetic and epigenetic abnormalities. To facilitate pluripotent reprogramming, we have identified the thyroid hormone triiodothyronine (T3) as an endogenous factor that can enhance reprogramming of human dermal fibroblasts (HDF) and umbilical cord mesenchymal stem cells (UCMSC). This potentiation of iPSC induction is associated with metabolic remodeling activity, including upregulation of key glycolytic genes, an increase in cell proliferation, and the induction of mesenchymal-epithelial transition (MET). We further identify the activation of the PI3K/AKT signal pathway by T3 as an underlying mechanism for the enhanced conversion to cell pluripotency in this model. These studies demonstrate that T3 enhances metabolic remodeling of donor cells in potentiating cell reprogramming. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据